argP+, argPd-S94L, argPd-P108S, argPd-P274Sfragment downstream of the phage T7-promoter, such that the encoded proteins beara C-terminal His6-tag provided by the vector DNA sequence. Theresultant plasmid was transformed into strain BL21(DE3) which has the T7 RNA Polymerase under the isopropyl thio-β-D-galactoside (IPTG) inducible lacUV5promoter.The resultant strains were grownin LB (500-1000 ml) to an A600of around 0.6and were then induced with 1 mM IPTG and harvested after 4-hrs of induction.Bacterial cells were recovered by centrifugation, resuspended in 20 ml of lysis buffer(20 mM Tris-Cl, pH-8; 300 mM NaCl; 10 mM DTT and 10 mM imidazole) containing20 μg/ml lysozyme, and lysed by sonication with 30-sec pulses for 10-min. Theprotocol for His6-ArgP(ArgPds)protein purification involved (i) passing the lysate through a 5ml Ni-NTA (Qiagen) chromatographic columnequilibrated with lysis buffer, (ii) washing thecolumn with 100 ml of washing buffer (20 mM Tris-Cl, pH-8; 300 mM NaCl; 10 mMDTT; 30 mM imidazole), and (iii) elution of His6-ArgP(ArgPds)from the column with elutionbuffer (20 mM Tris-Cl, pH-8;300 mM NaCl; 10 mM DTT and 250 mM imidazole) andcollection of 1.5 ml eluate fractions (10 fractions). The fractions were tested forprotein by Bradford method and the protein-carrying fractions (generally tubes 2 to 5)were pooled and dialysed in a 1:200 volume ratio against 20 mM Tris-Cl, pH-8 with 10mM DTT, 300 mMNaCl for 5 hrs followedby a change to buffer of composition 20 mM Tris-Cl, pH-8 with 10 mM DTT, 300 mM NaCl and 40% glycerol for 24 hrs. The proteins were concentrated by centrifugation toaround 1 mg/ml by using Amicon filter (pore size 10-KDa) and stored at −20ºC or −70ºC
- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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digitalhumanities.org:3030 digitalhumanities.org:3030
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The TEI's adoption as a model in digital library projects raised some interesting issues about the whole philosophy of the TEI, which had been designed mostly by scholars who wanted to be as flexible as possible. Any TEI tag can be redefined and tags can be added where appropriate
Question - What were some of the issues that arose with the TEI's adoption as a model in digital library projects?
The ability to add tags where needed throughout the texts seems to be a positive aspect due to the added ease of searching key words and it being included in said search.
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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After 2-3 h exposure,phosphorimagerscreenwas scannedon Fuji FLA-9000 to acquire hybridization images. Next,signal intensity for each spot on the membrane for both input and outputsampleswas quantifiedusing Fuji Multi Gauge V3.0 software andpercentage intensity foreach spot relative tothe whole signal intensity ofthe membranewas determined.To identify mutants with altered survival profiles,ratio of output (Op) to input (Ip) signal for each spot (oligonucleotide tag)present on the membranewas calculated.Mutantsdisplaying at least 6-fold higher and 10-fold lower survival were selectedas “up’ (Op/Ip= 6.0) and ‘down’ (Op/Ip = 0.1) mutants, respectively
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24 h post infection, THP-1 macrophages were washed thrice with PBS, lysed in water and recovered yeast cells were used to infect THP-1 cells at a MOIof 1:10. Three rounds of macrophage infection foreach mutant pool were carried out to enrich for the desired mutants in the final population. The lysate of 3rdround infection was inoculated in YPD medium for overnight (output). Cells were harvested, genomic DNA isolated from each input and output cell pellet andunique signature tags were PCR-amplified with P32-labeledα-dCTP using primers complementary to theinvariant region flanking each unique tag sequence. LabeledPCR products were denatured at 95°C for 10 min, chilled on ice and were hybridized tonylon membranescarrying immobilized plasmid DNA containing 96 unique tagsfor 14-16 h at 42°C.Membranes were washed twicewith 0.1X SSC bufferand exposed to phosphorimager screen for 2-4 h. Radioactive counts for each spot were quantified using Image Quant and Fuji Multi Gauge V3.0 software. Relative percentage intensity for individual spot was calculated with respect to allspots present oneach hybridizedmembrane
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YPD-grown cultures (0.05 OD600) of each mutant pool (96 mutants, each carrying a unique signature tag) were either inoculated in YPD medium for overnight (input) or used to infect differentiated THP-1 cells (1X106). After 2 h incubation, non-cell-associated yeastcellswere removed by washing THP-1 cellsthricewith PBS. At
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link-springer-com.ezp3.lib.umn.edu link-springer-com.ezp3.lib.umn.edu
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Afteractivating Hypothes.is in the browser, a learner can annotate any piece of text on a webpage with her own ideas, to whichother learners could respond (Principle 1). Tags can be attached to a Hypothes.is annotation, enabling the aggregation of webannotations that are scattered across webpages through a tag (Principle 2). Because Hypothes.is adheres to the Open AnnotationModel, annotations are uniquely identifiable and retrievable, allowing portability of ideas not widely supported in threadeddiscussion forums (Principle 2). The search functionality of Hypothes.is offers means to aggregate annotations according to avariety of criteria such as by tags, users, user groups, and annotated web URLs. Users are thus able to enter the discourse notonly from a specific webpage (e.g., the textbook), but also from the search page that provides another view of the discourse(Principle 3)
Alignment statement for Hypo
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The following antibodies were used in the present study:Primary antibodies against GAPDH (anti-rabbit), FLAG (anti-mouse), Immunoglobulin (IgG, anti-rabbit or anti-mouse),profilin-1 (anti-rabbit), tubulin (anti-mouse) and ubiquitin (anti-rabbit) were obtained from Sigma Aldrich Chemicals(St Louis, MO, USA). Antibodies againstAKT (anti-rabbit), cleaved caspases-3, 8 and 9 (anti-rabbit),HA-tag(anti-rabbit), Myc-tag (anti-rabbit), p21 (anti-rabbit), phospho-p53 (anti-mouse), PTEN (anti-mouse), phospho-AKT (Ser473; anti-rabbit), phospho-GSK-3β (Ser9; anti-rabbit), phospho-IKKα/β (Ser177/181; anti-rabbit), phospho-IκBα (Ser32; anti-rabbit), and phospho-p65 (Ser276; anti-rabbit) were obtained from Cell Signaling Technologies(Danvers, MA, USA), whereas antibodies for cox-2 (anti rabbit), c-Rel (anti-rabbit), ICAM-1 (anti-rabbit), IKKα/β (anti rabbit), IκBα (anti-rabbit), Mdm2 (anti-rabbit), PARP-1/2 (anti-rabbit), Rel-B (anti-rabbit), p50 (anti-rabbit), p53 (anti-mouse), p65 (anti-rabbit) were obtained from Santa Cruz Biotechnology(Santa Cruz, CA, USA).HRP (Horse radish peroxidase)-conjugated secondary antibodies (anti mouse and anti-rabbit) were obtained from Bangalore Genie(Peenya, India). For immuno-fluorescencestudies, secondary antibodiesconjugated toAlexa Fluor (488 and 594, anti-mouse and anti-rabbit) were obtained from Molecular Probes, Invitrogen(Eugene, OR, USA)
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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immunoprecipitation by using substrate specific antibody or pull-down by affinity trapping the substrate tag. The IP/pull-down complexes wereanalyzed by detecting the ubiquitination of substrate protein by using either substrate specificantibody or ubiquitin antibody through western blotting
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using gateway cloning method (Invitrogen). P73domain deletions were cloned in SFB destination vector. WWP2, WWP1, HACE1, E6AP, and PPM1G were cloned into SFB (S-protein/Flag/streptavidin binding protein (SBP) triple tag), GFP,and Myc mammalian destination vectors using the Gateway cloning technology (Invitrogen). WWP2 domain deletions were cloned into Myc-destination vector. WWP1 domain deletions were cloned into SFB-destination vector. PPM1G domain deletions were cloned into SFB mammalian destination vector using Gateway cloning. Bacterially expressing GST-p73, GST-∆Np73, GST-PPM1G, MBP-WWP1, MBP-WWP2, GST-WWP2, GST-WWP1 and GST-HACE1 were generated by using gateway technology. Ubiquitin WT and all the mutants were cloned into hemagglutinin (HA) mammalian destination vector. Flag-tagged Dvl2was purchased from Addgene. Dvl2 domain deletions were cloned into SFB-destination vectors. All the plasmid constructs generated in the present study are mentioned in table 2.Table 2: Plasmids used in the study
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web.hypothes.is web.hypothes.is
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ability to take notes anchored to specific passages of text, and have them tagged and searchable across papers, are such major benefits
For this to happen, start with:
- Open an article in the web browser (Google Chrome or Firefox) that can support pdf.js or epub.js or html if that is available
- Open hypothesis app, and start annotating at passages (as in this annotation)
- Add a consistent tag (see the other how_to tagged articles)
- Then search all related hypothesis annotations and notes on pages using those tags and organise knowledge that way.
Tags
Annotators
URL
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web.hypothes.is web.hypothes.is
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agree on a standard set of tags to classify sets of resources
This is step one. Set up a standard set of tags. These tags will then classify resources. Let's say I want to classify all studies on polygenic risk scores on the web. These documents come in the form of HTML, or PDF. If on the top of that, you'd also like to tag something like tutorial, or software to do the job, then write those tags as well when you come across a resource and tag it. So, a list of tags to learn about polygenic risk scores could be something like:
- polygenic risk score
- polygenic_risk_score
- tutorial
- utility
- why_how So, all resources that pertain to polygenic risk scores can now be divided into a set of main document types: some that discuss tutorials and how tos, others discuss the utilisation values and debates. Later, these could be reassembled.
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www.bbc.com www.bbc.com
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Get the news that’s local to you
As public tag
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annotatingausten.sfsuenglishdh.net annotatingausten.sfsuenglishdh.net
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dressing-room
A room primarily used during one's morning routine for dressing and washing. A woman's dressing room was made to be private and comfortable, and the intimacy of these small places allowed women to entertain small parties of other female guests. The wealthier the woman, the more luxurious her dressing room.

https://janeaustensworld.wordpress.com/tag/18th-century-dressing-rooms/
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workbook.craftingdigitalhistory.ca workbook.craftingdigitalhistory.ca
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emember then: make your annotations in our HIST3814o group!
Is this how to post to the group? add a tag?
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- Apr 2019
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gutenberg.net.au gutenberg.net.auSanditon2
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seminary
Austen is referring to a boarding school attended by young women from wealthy families who, for some reason, were not educated at home by a governess. https://judeknightauthor.com/tag/girls-education-in-regency-england/
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any complaint which asses' milk could possibly relieve
Donkey milk was considered a viable medical treatment from antiquity (Cleopatra bathed in it) until the turn of the 19th century, when it largely went out of fashion. It was considered a generic cure for a variety of conditions, including gout, scurvy, coughs, colds and asthma. For many, donkey milk caused stomach problems and "lactose intolerance."
Tags
Annotators
URL
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images.shanti.virginia.edu images.shanti.virginia.edu
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Bhutan Bhutan Cultural Library
KMap tag: type designation - icon, popover icon available
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audio-video.shanti.virginia.edu audio-video.shanti.virginia.edu
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Amdo Group 654 For more information about this term, see Full Entry below. SubjectsLanguage TreeSino-TibetanTibeto-BurmanBodicBodishTibetanFull EntryRelated Subjects (7)Related Texts (12)Related Audio-video (1864)
KMaps tag: bold, with popover.
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www.imsglobal.org www.imsglobal.org
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Annotation Profile Follow learners as they bookmark content, highlight selected text, and tag digital resources. Analyze annotations to better assess learner engagement, comprehension and satisfaction with the materials assigned.
There is already a Caliper profile for "annotation." Do we have any suggestions about the model?
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decollected.net decollected.net
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“touch”
From HUGHES AND GEDULD 202: the game of "tag" in Britain
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Local file Local file
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1) We identify and investigate two major kinds of lin-guistic activities on Quora: user level [e.g., basicactivities like posting a question/answer/comment aswell as linguistic styles that involves word/char usage,and part-of-speech (POS) tag usage] and questionlevel [e.g., content, topic associations, and edits for aquestion). Remarkably, many of these activities arefound to have a natural correspondence to the qualitiesthat human judges would consider while deciding if aquestion would remain unanswered (see Table I for a setof motivating examples).2) We perform an extensive measurement study to showthat answerability can be indeed characterized based onthe above-mentioned linguistic activities.3) A central finding is that the language use patterns ofthe users is one of the most effective mechanisms tocharacterize answerability.
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vdare.com vdare.com
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appears
The link is to my blog, but is defective. (a link that works is this, but this is to all occurrences of a tag.) Elsewhere, I found the page that the author was attempting to cite. Here I am not. The text is confused. I have never seen Oliver write "under his brother's name," but he has mostly claimed his brother is different, and then he claimed that he was lying about that and had been lying for years, then he took it back. Liars lie, no way around it. If a liar says "I'm a liar," a sane answer is "Not always." After all, stopped clock.
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claim he made this up.
This is a link to my blog, the URL is broken, though. The core points to this, a tag. Searching that display for "schizo," I find eventually material copied from this page: Authentic Darryl Smith on himself That page is Darryl making the claim of "made up." Recently, socks on Reddit -- probably also Darryl -- have repeated the claim that Oliver denied "schizophrenia," but I have never seen Oliver openly deny it. And it makes sense.
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success.docker.com success.docker.com
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If this is a production situation, and security and stability are important, then just "convenience" is likely not the best deciding factor (any more than leaving your house unlocked all the time might be "convenient").
如果这是生产情况,安全性和稳定性很重要,那么“便利”可能不是最好的决定因素(不仅仅是让你的房子一直解锁可能是“方便的”)
- 您可以考虑将每个push to registry的版本 - 以某种形式(毕竟,您发布了新版本的代码,并使其他人可以访问)。
- :latest与Git存储库中的master分支相当。是否每个
push to master都考虑准备投入生产? - Releases将(通常)通过验证过程(CI/QA /acceptance/etc)。是否应首先验证master中的更改,并且仅在验证(标记并)部署到生产之后?
- 发行版(Releases)带有版本;这可以是显式版本(标记),也可以是隐式(不可变标记:图像的摘要)
显式版本 -- image tag<br> 隐式版本 -- 不可变标记 :image digest
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However, there is not a 1:1 relation of digests to tags, so when pulling an image by digest, only the digest is known. If you happen to have an image pulled (manually) with a tag that matches that digest, the tag is shown, but not otherwise
但是,摘要与标签之间没有1:1的关系,因此在通过摘要pull image时,只知道摘要。如果您碰巧使用与该摘要匹配的标记(手动)拉出图像,则会显示标记,否则不会显示
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scalar.usc.edu scalar.usc.edu
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Impact of the Legion 9 plain 2019-04-16T02:18:41+00:00 Joseph Brown 5eac7ef3705d9f80e567b77e809a27064bd00249 1 2019-04-11T22:00:35+00:00 Joseph Brown 5eac7ef3705d9f80e567b77e809a27064bd00249 Organization of the Legion
As far as I can tell, you are using the tag function for the purpose of forward and backward movement. I'd reconsider using it for this purpose.
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“the expression or application of human creative skill and imagination […] producing works to be appreciated primarily for their beauty or emotional power.”
Who is the speaker on this quote? Braidotti? I would tag the source or do what you did above (Author last name, page #)
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scalar.usc.edu scalar.usc.edu
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Alexander McQueen: Relationality between human and non-human Charles Sirisawat-Larouche 6 plain 2019-04-10T17:03:44+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-01T13:48:01+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 The Deconstructivist Approach Charles Sirisawat-Larouche 22 plain 2019-04-10T16:51:05+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-10T11:43:52+00:00 Alexander McQueen: Relationality between human and non-human 6 plain 2019-04-10T17:03:44+00:00 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-10T13:46:55+00:00 The display of its fashion is done toward alien like figure or cyborg incarnation which allow a certain extent of dis-identification. 3 plain 2019-04-10T13:48:27+00:00 1 2019-04-10T12:26:35+00:00 Fashion and Anti-fashion Dichotomy 2 plain 2019-04-10T16:34:01+00:00 1 2019-04-10T12:22:36+00:00 Phallogocentrism 3 plain 2019-04-10T16:38:25+00:00 1 2019-04-10T12:26:06+00:00 Self-Styling and Self-Fashion 3 plain 2019-04-10T16:39:08+00:00 1 media/Fashion-Looks-Forward_Utopian-Bodies_Exhibition_Stockholm-_dezeen_936_12.jpg 2019-04-01T15:44:50+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Post-Gender Charles Sirisawat-Larouche 15 visual_path 2019-04-10T17:26:43+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-10T00:30:04+00:00 Rei Kawakubo and the excessive use of material as a strategy to reassert the conceptual embodied entity toward subversive site. 5 Rei Kawakubo and the excessive use of material as a strategy to reassert the conceptual embodied entity toward subversive site. Click and Drag to Annotate plain 2019-04-10T16:17:23+00:00 1 media/IMG_5473 2.jpg media/Animated GIF-original.mp4 2019-04-01T13:55:26+00:00 Thierry Mugler: The contradictory abjection 27 visual_path 2019-04-10T17:32:51+00:00 1 media/2000px-Polka_dots.svg.png media/Animated GIF-original.mp4 2019-04-04T13:16:11+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Posthuman Museum Practices: From Runway to exhibition Charles Sirisawat-Larouche 34 Preceding the essence of fashion tags 2019-04-10T17:47:08+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-08T15:25:59+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Self-organizing: Curator, Curatorial. Curating... Charles Sirisawat-Larouche 10 visual_path 2019-04-10T14:09:14+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 media/IMG_5473 2.jpg media/Animated GIF-original.mp4 2019-04-01T13:55:26+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Thierry Mugler: The contradictory abjection
Nice idea to use tags. Relevant to the style of assignment.
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ltcdev.uow.edu.au ltcdev.uow.edu.au
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Can we at this point have the option of also adding a "TAG" function It's much faster to be able to tag as when new questions are being inputted
Also if the question TAG is shown here, new quizzes can be created on the spot with a specific question TAG (if that makes sense)
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landarts.org landarts.org
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Utility friendships
tag urself this is me and masooch
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onlinelibrary.wiley.com onlinelibrary.wiley.com
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nlx_152244
Curator: @gabimpine
Curator note: Tag is for the company
Resource used:
(Abcam, RRID:SCR_012931)
SciCrunch record: RRID:nlx_152244
Alternate resolvers: SciCrunch xml N2T identifiers.org
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nlx_152487
Curator: @gabimpine
Curator note: Tag is for the company
Resource used:
(Wako, RRID:SCR_013651)
SciCrunch record: RRID:nlx_152487
Alternate resolvers: SciCrunch xml N2T identifiers.org
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facdevblog.niu.edu facdevblog.niu.edu
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Mobile learning naturally brings smartphones to mind, like the iPhone or an Android-powered pho
What qualifies as a mobile device and what activities/techniques are possible?
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www.foxnews.com www.foxnews.com
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Safety advocates urged college students to match the vehicle color and model, the license tag number, and the photo of their ride-share drivers before getting in a vehicle and make the driver say their names to them before they introduce themselves.
Students should follow this to be safe.
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Safety advocates urged college students to match the vehicle color and model, the license tag number, and the photo of their ride-share drivers before getting in a vehicle and make the driver say their names to them before they introduce themselves.
Always make sure to do this
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onlinelearningconsortium.org onlinelearningconsortium.org
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Online Learning Consortium (OLC)
Hello OLC!
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www.insidehighered.com www.insidehighered.com
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But the offer came with a hefty price tag, the negotiators said, which the system was not willing to pay.
Doesn't it seem like everything comes with a hefty price these days...
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digitalempathyvet.com digitalempathyvet.com
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Dr. Sanha Kim should be moved over to Columbia. We need to add a space holder for Cynthia Loveland under Columbia doctors. For all doctors except Tanya Tag, list them as 'Emergency Veterinarian' instead of emergency and critical care. Under 'doctors' Dr. Tag looks great, could you add Towson & Columbia next to Medical Director? For Dr. Tag under administration, remove her middle initial and change DVM to match DACVECC as displayed for her under doctors. Leave medical director Towson & Columbia. Please display leadership in this order: Angelina Morgan, Tanya Tag, Tamara Santesse, Elizabeth Moyers, Beth Faux, Ashley Carlson, Victoria Syczurowski, Jeff Hawkins, Christine Wolff, Paul Heggie, Joey Sumby (I spelled his name wrong last time)
Beth Faux should display as "Beth Faux, RVT, VTS (ECC). Her title should be listed as Technician Manager Columbia. Tamara Santesse's title should be Office Manager, Towson. Elizabeth Moyers' title should be Office Manager, Columbia Victoria and Ashley's title are: Technician Co-Manager Towson Jeff Hawkins' title should be Financial Manager Towson & Columbia Paul Heggie's title should be Facilities Manager Towson & Columbia Joey Sumby's title should be Facilities Supervisor Columbia
Love the super heroes-are there gender specific?
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- Mar 2019
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alexan25.myhostpoint.ch alexan25.myhostpoint.ch
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Twitter Facebook Instagram Impressum Haftungsausschluss
Alignierung kann angepasst werden
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- gutes Teaserbild. Allerdings nimmt zuviel Platz. Für den Hauptinhalt (Wanderungen) muss man sofot scrollen. Eine Alternativ wäre mit einem "Karrussell" von den letzten 3 Wanderungsbilder oder so.
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www.learning-theories.com www.learning-theories.com
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Behaviorism
Learning-Theories.com published a very handy few pages that describe various learning theories. This is a quick, straightforward, simple way to access information on the different theories. This article, Behaviorism, explains that the theory assumes learners learn by responding to external stimuli in their environment. Learning under behaviorism is characterized by a change in the learner's behavior. I use this in my horse training as I use both positive reinforcement (clicker training) and negative reinforcement (pressure-release) to structure my horse's behaviors. Behaviorism can be translated to human work, too. I've used TAG teaching (clicker training for humans) to teach people to get on and off horses with ease and also to trim horses' hooves. I also use it to clicker train my cats! 6/10
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Republicans immediately painted the plan as “disastrous,” pointing to studies that suggest the price tag could be as high as $32 trillion.
Source?<br> Which Republicans?
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digitalempathyvet.com digitalempathyvet.comPricing1
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health exam and vaccinations - $167.53 includes Doctor exam, rabies, Dhlpp, bordetella, send out to lab fecal, and heart worm occult and parish tag. puppy care 1st shots= $137.40 2nd- 9 weeks is $143.29 3rd - 12 weeks $147.01 4th - 15 weeks $187.08 Micro chips- $63.13 feline neuter- $100.89 feline front declaw- $232.87
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web.hypothes.is web.hypothes.is
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disallows it in our Public channel and in groups
You can, however, add a tag to a highlight in order to share it to the Public channel or a group.
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elearninginfographics.com elearninginfographics.com
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personalize learning infographic
This is not quite what it sounds like. It is a Pinterest style page with links to assorted articles that relate to personalized learning, most of which are presented in an infographic. It is sufficiently useful if one has the patience to click through to the infographics. Usability is satisfactory although the top half of the page is taken up with graphics that are not directly related to the content. rating 3/5
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www.allisonrossett.com www.allisonrossett.com
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This plain page incorporates an overview of job aids by Allison Rossett, who is the foremost authority on the topic. Not all information is given away for free as she wants to sell her books, which are also promoted on the page. This page can be a good way of tracking her current work. Rating 3/5
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internal-developer.veevavault.com internal-developer.veevavault.com
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LATESTVERSION
code tag here
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typito.com typito.com
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Create Stunning Videos Online
Templates by TYPITO
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theladyalliance.com theladyalliance.com
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CourseFolk
I've taken the focus off CourseFolk being educators who craft the lessons and made that something The Lady Alliance does and CourseFolk just does the techy website/members stuff. I think influencers will balk at a random company doing the lesson writing part for them. I'm still happy to do the interviews and workshop the lessons, but maybe I can do that with a Lady Alliance tag somehow. We can chat more about how this will work
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- Feb 2019
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arxiv.org arxiv.org
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the agent model swaps the in-put and output sequences, and it also takes the tag of filledinformation slots as an input which is extracted from dia-logue in previous turns by pattern matching with the orderinformation in ground truth
agent model 构建前先预训练。网络结构和user model一样,但是输入和输出反转,同时也把之前对话中已经填充的槽位信息作为输入。但是这俩部分信息并不是简单的直接拼接在一起,而是来学习适合的attention 权重来更好的利用注意力机制。此外任何其他额外的语义意图标签都不必用。
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URL
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www.wired.com www.wired.com
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And who will write the software that makes this contraption useful and productive? We will. In fact, we’re already doing it, each of us, every day. When we post and then tag pictures on the community photo album Flickr, we are teaching the Machine to give names to images. The thickening links between caption and picture form a neural net that can learn. Think of the 100 billion times per day humans click on a Web page as a way of teaching the Machine what we think is important. Each time we forge a link between words, we teach it an idea. Wikipedia encourages its citizen authors to link each fact in an article to a reference citation. Over time, a Wikipedia article becomes totally underlined in blue as ideas are cross-referenced. That massive cross-referencing is how brains think and remember.
This was the main goal of the 'docuverse' where information will be cross referenced and everything will be related in someway to everything else.
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tag those and only those who will really derive benefit
Implies you know who those people are and you may not know but for some of us that is the whole point of an ESN
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Developing employees
An HBR tag for the article
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www.anxietycanada.com www.anxietycanada.com
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We're working on an improved
Do we just post to public or do we add a LDRS 626 tag?
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law.yale.edu law.yale.edu
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A study published in October of last year sought to determine how to make best use of digital out-of-home (DOOH) advertisements in the London Underground.7 An example of a DOOH ad would be a digital billboard programed to change the advertisement on display after a specific period of time. To achieve their goal, the researchers used the same Twitter Streaming API described in the previous study; however, this time they utilized Twitter’s geotagging function (a capability that allows Twitter users to “tag” their location when they post a tweet). Each London Underground station was carefully outlined on a map of London. Then, the researchers randomly sampled geotagged tweets falling within those zones (meaning the tweeter was at a station). The specific Underground station, the time of the tweet, and the content of the tweet were all extracted. The researchers continued this practice for one year, seemingly unbeknownst to the Twitter-using patrons of the London Underground, collecting over 10.5 million tweets. This data was then compiled and processed to determine what sort of things people were tweeting about in each London Underground station at certain times of the day on weekdays and on weekends. For example, nearly 35% of tweets from the Holloway Road station were about sports, and almost 40% of tweets posted between 6 PM and midnight on weekends at the North Greenwich station were about music.8 The authors of the study recommended using this data to create targeted DOOH advertising. For instance, a music-related ad on a rotating digital billboard at night on the weekends in North Greenwich station would probably be more successful than an ad for a sports team.
This entire passage gives a perfect example of how the data can be broken down and used to make money by advertisers. I intend to allude to the fact that it doesn't stop here.
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www.w3schools.com www.w3schools.com
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<p>This is a paragraph.</p> <p>This is another paragraph.</p>
Paragraph Tag
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URL
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www.w3schools.com www.w3schools.com
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<a href="default.asp"> <img src="smiley.gif" alt="HTML tutorial" style="width:42px;height:42px;border:0;"></a>
Image as a link - Ignore the style="…." part of the tag. Inline styles are bad!
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<img src="https://www.w3schools.com/images/w3schools_green.jpg" alt="W3Schools.com">
Image Tag
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URL
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www.w3schools.com www.w3schools.com
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<a href="https://www.w3schools.com/html/">Visit our HTML tutorial</a>
Link Tag
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worrydream.com worrydream.com
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few
Is there a common tag that we are using for annotations here?
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www.insidehighered.com www.insidehighered.com
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it allows you to tag records
Since DH is such a collaborative subject, you have to make sure references are accurate so that everyone gets credit for their contributions
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- Jan 2019
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. The nofollow tag came about as a method to help stop automated blog comment, guest book, and link injection spam
Appreciated
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static1.squarespace.com static1.squarespace.com
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summary,
super helpful summary. Commenting just to tag it.
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URL
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static1.squarespace.com static1.squarespace.com
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posthumanitie
commenting just to tag this
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dhdebates.gc.cuny.edu dhdebates.gc.cuny.edu
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SGML, HTML, XML
SGML = Standard Generalized Markup Language, "a standard for how to specify a document markup language or tag set... SGML is not in itself a document language, but a description of how to specify one." So a set of guidelines, like TEI?
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www.nytimes.com www.nytimes.com
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$10 billion is a hefty price tag.
however Dr. Hossenfelder does not agree the cost outweighs the benefits
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With a $5 billion price tag and a $1 billion annual operation cost, the L.H.C. is the most expensive instrument ever built
That is intense. 5 billion $ to build, yet it cost 1 billion to upkeep.
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ucdenver.instructure.com ucdenver.instructure.com
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. Social Media is where we will connect with the broader digital storytelling community, which are very active on Twitter and Instagram. You may participate anonymously if you prefer. Tag all class related communications with #inte5340 (Links to an external site.)Links to an external site. and #digstory (Links to an external site.)Links to an external site. .
Would using our personal social media be acceptable for public communication or would you suggest in creating a new account?
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Articles and videos will be assigned each week.
Just wondering what the weekly deadline for discussion will be. Also, do I need to tag these comments...I just saw the option below?
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Tag all related posts
So, we will not be sharing the blog directly on canvas, only tagging our posts correct?
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Astros outfielder Josh Reddick is a big wrestling fan, so after he tied the knot this weekend, him and his bride came out to their friends and family using the same entrace as current WWE tag team champions Bobby Roode and Chad Gable.
"him and his bride came out to their friends"
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www.ontotext.com www.ontotext.com
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Tag Archives: linked open data
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hypothes.is hypothes.is
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Machines with interchangeable parts can now be constructed with great economy of effort. In spite of much complexity, they perform reliably. Witness the humble typewriter, or the movie camera, or the automobile. We have reached a point in technology now where all of these inventions can perform their jobs without human involvement.
His description of the "humble typewriter" seems like such an understatement in comparison to the computers and recording devices we now have
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openedx.atlassian.net openedx.atlassian.net
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the-tag/you-want-to-install
open-release/hawthorn.2
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www.theatlantic.com www.theatlantic.com
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burying their old professional competition in the demand of a common cause, have shared greatly and learned much. It has been exhilarating to work in effective partnership. Now, for many, this appears to be approaching an end.
What is interesting is how individuals still share their knowledge to create new things, artists and scientists collaborating together, but how there is still that tight restriction that controls what can be done. New inventions, artworks, music, and tools are still copyrighted and individuals must apply to work with them. You also have inventors that place an outrageous price tag on some of their products, limiting the number of people who can buy them and find out what things they can do that are beyond even the mind of the tool's creator.
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crmintler.com crmintler.com
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Hypothesis Annotation instructions:
1) Make 2-3 annotations. 2) If another student has already annotated a passage you wished to annotate, you have two options: reply to that annotation or choose another passage to annotate. 3) Reply to at least 2 annotations made by your peers 4) If someone replies to your annotation, reply to keep the conversation going. 5) TAG all annotations CITA2019
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www.si.com www.si.com
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New York City Fire Department
Sample, notice how I made it public and used a tag. You can also add links to other material.
Images are good too, but only if you think they add something to our understanding of the text. Here's the first female firefighter in the NYFD.
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www.le24heures.fr www.le24heures.fr
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Proche de la basilique Saint Sernin, haut lieu touristique de la ville rose, le quartier Arnaud Bernard ne profite pas de ce rayonnement. Beaucoup de rues sont sales et le quartier n’attire pas les visiteurs. Des tags et des graffs décorent les murs de certains immeubles. Le quartier fait partie des tout premiers de la ville à accueillir de nombreux artistes graffeurs, mais sans organisation. En 1997 l’association « carrefour culturel » décide de lancer un projet avec les habitants du quartier: réaliser une fresque géante dans la rue Gramat pour couvrir les tags. Après trois ans de discussions avec la municipalité, les propriétaires et les artistes, la fresque peut enfin voir le jour. Les riverains participent eux-mêmes au nettoyage de la rue pour accueillir les dessins. Elle est au cœur d’un projet citoyen. David Brunel explique : » le but c’est de créer à l’époque des actions culturelles où les citoyens sont à l’initiative, mais aussi acteur du projet du début à la fin » Il poursuit : » le quartier est vu comme une base d’expérience, avec une réflexion sur le civique ». Parmi les artistes, on retrouve un SDF du quartier, un peintre mais aussi des gens venu d’Espagne. Ils sont tous là pour donner une vision de la ville et de son histoire. Plus qu’une fresque ce sont des échanges, un projet construit par les habitants, pour les habitants. Annick Lodereau, l’une des meneuses, résume le projet à l’époque : » C’est une fresque anti-tag, une création qui démontre que l’on peut faire des choses ensemble et leur donner un sens ». Malgré l’ampleur du projet, au fil des ans la rue est de nouveau utilisée par les tagueurs qui repassent sur les fresques de l’époque dont il ne reste aujourd’hui plus rien.
Fais des retours ligne pour marque les paragraphes, sinon c'est trop difficile à lire.
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unify.id unify.id
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URL
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wendynorris.com wendynorris.com
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In this section of the paper we broach two aspects of this articulation issue, onefocusing on the management of workflow, the other on the construction and manage-ment of what we term a ‘common information space’. The former concept has beenthe subject of discussion for some time, in the guise of such terms as office automa-tion and more recently, workflow automation. The latter concept has, in our view,been somewhat neglected, despite its critical importance for the accomplishmentof many distributed work activities
A quick scan of ACM library papers that tag "articulation work" seems to indicate the "common information space" problem still has not attracted a lot of study. This could be a good entry point for my work with CSCW because time cuts across both workflow and information space.
Nicely bundles boundary infrastructure, sense-making and distributed work
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- Dec 2018
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vitalirosati.net vitalirosati.net
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inscription
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URL
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spilledreality.tumblr.com spilledreality.tumblr.comOtessa1
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But the real sex politics are more ingrained and foundational, relating to how ‘Tag perceives herself in the world and how that self-image as object lends itself to a specific and perhaps primarily female mode of suffering.
A. Saramandi: "She’s victim of a culture that bombards her with the message that she is in control, as long as she buys the necessary products, looks a certain way. In one of the most poignant moments of the novel, she doesn’t realise that this agency is a false god: when the narrator is molested by one of her father’s colleagues, she believes she was “letting [him] kiss me” and is confused as to why, since after all, as the epitome of desire, she must be responsible."
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surfacescholar.com surfacescholar.com
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The real question, I realized, was: how can I remember anything if I can only keep it in one place?
The biggest issue I have with iThoughts right now is the lack of ability to dynamically filter maps based on tags or other properties of nodes. I'd love to be able to view only the nodes I need for a specific query and have everything else dynamically vanish— particularly on large maps, where the existing method of filtering renders the nodes illegible...
That said, I've come to depend on text searches to return a filtered list of nodes. iThoughts allows for searching within a map and across all maps in the filesystem. It's not a fuzzy search, so search strings have to be constructed in a fixed way which necessitates a robust system of memorable tags, categories or conventions (for example, use of the tag "@due(2019-10-02)" allows me to filter for nodes with due dates, and nodes due within a specific year, month or on a specific date, by parsing incremental fragments of the tag. Simple, but effective.
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adullact.net adullact.net
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Tag Cloud
Le nuage de mots surcharge la page
Un nuage de mots n'apporte pas beaucoup d'informations aux utilisateur·rice·s qui arrivent sur cette page.
En revanche, cela surcharge la page à la place d'autres informations qui pourraient être plus utiles.
Suggestion : placer ce nuage de mots sur une page spécifique que les utilisateurs que ce nuage de mots intéresse pourront consulter.
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web5.lib.pacificu.edu web5.lib.pacificu.edu
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seine Grundschulen
nachdem sie am Tag von der Grundschule fertig sind
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science.sciencemag.org science.sciencemag.org
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We therefore developed protocols for fluorescent in situ hybridization (FISH) targeting specific ascomycete and cystobasidiomycete rRNA sequences.
A molecular probe is created against a specific DNA sequence, in this case rRNA from either ascomycetes or basidiomycetes. The probe is designed with a recognizable tag on the end. Then a second probe is used to match the first probe, and finally a third probe to match the second one with a fluorescent tag on it. By using multiple probes we can amplify even the smallest signals to our first probe. These probes are then mixed with a biological sample that is fixed in place. If fluorescence is observed the probe has found a target.
In some experiments, as in this example, researchers use multiple probes in a single experiment. This provides information about how close two organisms of interest are in a given sample.
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www.huffpost.com www.huffpost.com
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Pong in 1972 and Space Invaders in 1978, which kick started a cultural revolution. Notably in 1981, with Nintendo’s masterpiece, Donkey Kong
These were the first games to really kickstart video games' jump into popular culture. More than that, they left their mark on society as the first games to be accessible in your homes, which is an important note. That was the start of gaming being right in people's faces, as they have become everywhere now.
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gutenberg.net.au gutenberg.net.auSanditon4
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equipage
An equipage is an archaic term for a horse drawn carriage and its attendants. https://www.merriam-webster.com/dictionary/equipage
Here is an article about regency transportation (including video links): https://janeaustensworld.wordpress.com/tag/regency-transportation/
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seen romantically situated among wood on a high eminence at some little distance
This description of a cottage reminds me of the contrast in Austen's Sense and Sensibility between how the upper classes and the landed gentry view cottages. The upper classes view cottages in a romantic way as cute, comfy homes, however the landed gentry know that cottages result out of a neccesity brough on by an oppresive and restrictive economic system.
https://janeaustensworld.wordpress.com/tag/19th-century-cottages/
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leeches
"Bloodletting procedures, including leeching, became the most common medical procedure throughout the early modern period. By the early 19th century, many patients regularly submitted to various bloodletting practices as a means of preventing or treating infection and disease"
https://www.britannica.com/science/leeching
Leeching more specifically in Jane Austen's world: "One can imagine that during her final illness, Jane Austen was no stranger to leeches."
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machine
"Wagons, called Bathing Machines, were invented especially for the purpose, and would be drawn out into the water by sturdy women, who might then assist you down into the water where you could paddle about or swim in relative privacy, shielded from view of the shore."
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jrnl.global jrnl.global
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KnowFlow in a visual knowledge management enviroment for collaborative groups. Collaborative SAAS, purely web-based multi-user application.
Although it is been created as a part of Neuroweb platform, it seems, that roots behind Douglas Engelbart conceptual framework and Neuroweb conceptual framework are the same. As one can see when reading D.Engelbart's group documents, cognitive science concepts were used in a context very similar to what is considered to be a conceptual framework of Cultural-historical psychology approach, based on undestanding of concept developement and role of symbolic representations in development of psyche and culture. One can see citations of authors connected to cultural-historical psychology throughout the works of Engelbart's group, i.e. of Alan's Keys and other members. KnowFlow system as it is and in scope of it's developement is a system that can easily be understood via DKR conceptual approach. It does not mean that it was build on D.Engelbarts framework alone, but still all the basic concepts of DRK are there. We build KnowFlow to be a living knowledge network, providing means to import, create, modify and view and export knowledge in a process of any complex product or project developement by a group of knowledge workers. That is basically a tool to boost group's IQ, its collective exocortex. KnowFlow can import different kinds of documents, including texts, maindmaps, presentations, media materials. We can do it via direct import, import from connected sources via public API, or via WebClipper that works as a Chrome Browser extention similar to one of Evernote, that can grab a page, fragment of text or a screenshot to save it as a knowledge element, rich format note. If the initial imported document is too large or consists of many subdocuments, special import algorythm can parce it up to a set of knowledge elements, saving each of them separately. Semantic engine is used to help user to parce the documents, finding the key statements in text, separating them and even instantly creating a concept-map our of the text.
User can work individually or with a group of other users on this knowledge material in different ways: He can work with element as a text, using simple text editor, adding additional materials to a body of the knowledge element, making a group of elements form one element or combining several elements into a group that can be presented as a group or as a complex element at the same time. One can also use element (card) templates to make his work easier. User can also work with his collection of knowledge as with a database, searching through elements, filtering them, group or combine them in a "collection view". One can use domain system to tag or classify elements of knowledge he is working with. Domain is a set of classes that can also me named ontology. So, user can place elements in context of different ontologies. User can work with elements or groups of elements (that can be seen as fractal elements) in a concept-map view. It means that one can connect elements with cinnection, set relations between them and work with them as one works with knowledge maps. One card (knowledge element) can be used in different maps, still being the same element, changing in all the maps it is being used it. Or elements can be copied or modified, That way the system will know who and how used elements to build a new knowledge upon them. Version control is also there. One can use his collection view not for his whole collection but for one knowledge space (map). This view disregards connections, still providing means to work with groups of cards and stacks or sets, similar to Kanban decs used in Trello (and yes we have an integration with trello. too) Knowledge spaces (maps) can be connected to each other through "portals" - these are cards (elements) or groups of cards that are present in several maps (knowlege spaces) at the same time. This way knowledge workers can create a multi-space flow of knowledge between different maps, belonging to one or many groups, working with different aspects of knowledge and different stages of its life-cycle. A special instrument - "Сass" can be used to import knowledge elements or portals from other maps during group work. Users can instantly view the map (knowledge space) in presentation view, when each element is one slide. navigating through complex knowledge structure, choosing different trajectories depending on what they want to focus on. Additional collaboration tools include, but not limited to voting, commentaries end collective editing of knowledge in any view, as well as using shared knowledge space templates for different kinds of collaboration (brainstorms, SWOT analisys etc), collaborators can share elements of knowledge, collections, maps or it branches between individual users, groups or make them publicly available.<br> All that work is augmented with voice recognition that lets users speak their knowledge in - and that is particularly useful in capruring debates or meetings. All functions of the KnowFlow system are augmented with machine learning semantic recommendation engine. That recommends fragments of knowledge already in the system, recommends templates and connections in knowledge map mode. We also have a Virtual Relity interface that can give one an opportunity to view multiple knowledge spaces at the same time, walk between knowledge networks and see them being created in real time.
All the results can be exported in any moment to a number of formats (including PDF, Mindmanager, Table, Text, etc) or streamed through API to other elements for additional ways of use. One of uses of KnowFlow is creation a templates or publically available knowledge spaces, knowledge elements, maps or chains that contain best practices and knowledge that can be used in other contexts, when needed.
Next version will also include timeline views usefull for sorting all the knowledge elements on a timeline. filtered by meta-data, VR interface will get edit capabilities and voice commands will be available for a group work in shared spaces. Additional libraries of semanic analisis will be added, text edit mode will be upgraged, and number of other software integrations will provide easy use of KnowFlow as a collaborative knowledge processing tool in any workflows.
That is, basically, DKR, as it seems to me.
“A dynamic knowledge repository is a living, breathing, rapidly evolving repository of all the stuff accumulating moment to moment throughout the life of a project or pursuit. This would include successive drafts and commentary leading up to more polished versions of a given document, brainstorming and conceptual design notes, design rationale, work lists, contact info, all the email and meeting notes, research intelligence collected and commented on, emerging issues, timelines, etc.” That are the forms of data that can be inmorted, used, transformed and contextually accessed, History tool provides ability to move back in time to a point in knowledge space developement, to access relevant data and fork new line of work from there, still being able to use all the knowledge being created later or in lateral lines of work, even by other groups, proven that access mode for them makes it available.
One can say that knowflow is created as a version of CoDIAK process, as it supports and speeds up ways to develop, integrate, and apply all this iterating knowledge from the swirl of disparate concurrent contributions. So, if KnowFlow is an “emerging collective record of all this activity captured on the fly -- the emerging collective vision, know-how, the group brain, memory, where the dots are connected and the right hand knows what the left hand is doing” it means that KnowFlow is really a version of DKR .
KnowFlow emerged as a result of best practices of work with project groups in business schools, methods and approaches to collective work (i.e. Rapid Foresight methodology, co-developed by KnowFlow team members, etc) and is already used to facilitiate the collective work “in a way that optimizes the capture, organization, and enhanced utility of the DKR”. Best practices would include special roles such as collaboration facilitators, as well as high performance knowledge specialists, who are continuously moderating the connectivity, capture, tagging, tracking, and portals into the emerging repository -- tools, practices, and people connecting in a way that renders the DKR optimally navigable, searchable, and useful.
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- Nov 2018
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imackinson.com imackinson.com
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It’
The apostrophe isn't rendering correctly because you're missing the meta charset tag in your HTML head.
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evanmillican.com evanmillican.com
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â€
The apostrophe didn't render correctly because you forgot the meta charset tag in the HTML.
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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only a small fraction of the neurons that receive odor information respond to each odor
Lin et al. discovered the neurological process that controls why so few olfactory neurons make up each odor "tag."
The reason is because when the cells that receive odor information, called Kenyon cells (KCs), become active, the anterior paired lateral neuron responds by slowing the activity of the KCs. The more active the KCs become, the harder the anterior paired lateral neuron works to block their activity. The few KCs that are left active after this process form the odor tag.
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rachaellhead.com rachaellhead.com
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it’s
This isn't rendering as an apostrophe because you forgot the meta charset tag in your HTML
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robinderosa.net robinderosa.net
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We have lost any sense of what we mean when we call a university “public.
It really loses the tag of public when it's hundred of dollars to take a single class almost anywhere.
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www.aclweb.org www.aclweb.org
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probabilistic model to tag English text
*
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www.makeuseof.com www.makeuseof.com
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Partial charges and discharges that combine to 100% are counted as a single full cycle
Really? I don't think anyone has tested partial charges and discharges. I would like to see a reference for this point!
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research says
That is pretty weak evidence for such a strong statement
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edegbor.digstonehill.org edegbor.digstonehill.org
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Blog Posts
This is kind of a catch all tag, like uncategorized. Try to keep your tags specific.
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www.technologyreview.com www.technologyreview.com
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Gates calls the new approach a “breakthrough,” but some environmental groups say gene drives are too dangerous to ever use.
trying to add a tag
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mpi4py.readthedocs.io mpi4py.readthedocs.io
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comm.Send([data, MPI.INT], dest=1, tag=77)
Capital functions are lcosest to their C equivalent
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Local file Local file
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lgjuraj.digstonehill.org lgjuraj.digstonehill.org
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Categories Uncategorized
You should have no uncategorized posts at this point. Start using tags and categories and back tag your earlier posts.
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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A-11122
Curator: @evieth
Curator note: Thermo Fisher Scientific Cat# A-11122, GFP Tag Polyclonal Antibody
Resource used:
(Thermo Fisher Scientific Cat# A-11122, RRID:AB_221569)
SciCrunch record: RRID:AB_221569
Alternate resolvers: SciCrunch xml N2T identifiers.org
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www.sciencedirect.com www.sciencedirect.com
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2278
Curator: @evieth
Curator note: Cell Signaling Technology Cat# 2278, Rabbit Anti-Myc-Tag Monoclonal Antibody, Unconjugated, Clone 71D10
Resource used:
(Cell Signaling Technology Cat# 2278, RRID:AB_490778)
SciCrunch record: RRID:AB_490778
Alternate resolvers: SciCrunch xml N2T identifiers.org
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academic.oup.com academic.oup.com
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IMSR_JAX:012 569
Curator: @bandrow @evieth
Curator note: IMSR Cat# JAX:012569
Curator note: The missing tag here is not correct. This RRID is there.
Resource used:
RRID:IMSR_JAX:012569
SciCrunch record: RRID:IMSR_JAX:012569
Alternate resolvers: SciCrunch xml N2T identifiers.org
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elifesciences.org elifesciences.org
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ab137839
Curator: @evieth
Curator note: Anti-6X His tag antibody, Abcam
Resource used:
(Abcam Cat# ab137839, RRID:AB_2732046)
SciCrunch record: RRID:AB_2732046
Alternate resolvers: SciCrunch xml N2T identifiers.org
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Annotators
URL
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bmcbiol.biomedcentral.com bmcbiol.biomedcentral.com
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mouse monoclonal anti-His tag (ABclonal Technology Cat#AE003
Curator: @evieth
Resource used:
(ABclonal Cat# AE003, RRID:AB_2728734)
SciCrunch record: RRID:AB_2728734
Alternate resolvers: SciCrunch xml N2T identifiers.org
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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G10362
Curator: @evieth
Curator note: GFP Tag Antibody, ABfinity(TM) Rabbit Monoclonal
Resource used:
(Thermo Fisher Scientific Cat# G10362, RRID:AB_2536526)
SciCrunch record: RRID:AB_2536526
Alternate resolvers: SciCrunch xml N2T identifiers.org
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www.sciencedirect.com www.sciencedirect.com
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RRID:AB_2099233
Curator: @evieth
Resource used:
(Cell Signaling Technology Cat# 7074, RRID:AB_2099233)
SciCrunch record: RRID:AB_2099233
Alternate resolvers: SciCrunch xml N2T identifiers.org
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- Oct 2018
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wikimediafoundation.org wikimediafoundation.org
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Oates soon realized navigating Wikimedia Commons and finding interesting materials is challenging. She noted how the category system used to organize and tag media files on Commons is confusing and hides—not shows—the richness of content there.
Problems with the category system making it difficult to search relevant information
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www.inter-logic.gr www.inter-logic.gr
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historiography
public test by marg with tag historians
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tud.sg.dev.code.one tud.sg.dev.code.one
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port From Doc
Tú coi a Phong tag nè
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www.lenr-forum.com www.lenr-forum.com
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agree, but "you can't prove a negative".
It's easy to mess up the quotation formatting. Also easy to fix with a close-quote tag. That errors like this commonly persist shows that people don't go back and read their own writing.
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editions.lib.umn.edu editions.lib.umn.edu
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Margaretta M. Lovell #pt-cv-view-29709a7js0.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-29709a7js0.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-29709a7js0.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; } #pt-cv-view-29709a7js0 .pt-cv-title a, #pt-cv-view-29709a7js0 .panel-title { font-weight: 600 !important; } #pt-cv-view-29709a7js0 .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-29709a7js0 .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-29709a7js0:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-29709a7js0 .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-29709a7js0 .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-29709a7js0 .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-29709a7js0 .add_to_cart_button, #pt-cv-view-29709a7js0 .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-29709a7js0 .pt-cv-readmore { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .pt-cv-readmore:hover { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-29709a7js0 + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-29709a7js0'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-29709a7js0'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-29709a7js0 li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-29709a7js0 .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .cvp-responsive-image[style*="background-image"] { width: 200px; height: 200px; } #pt-cv-view-29709a7js0 .pt-cv-ocol:nth-child(2n+2) .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Bully PulpitWhat Is the Role of Patriotism in the Study of American Art?M. Elizabeth Boone, Lauren LessingM. Elizabeth (Betsy) Boone and Lauren Lessing, Executive Editors Do we have a responsibility to the nation in our teaching and writing on American art? How would this responsibility be enacted and can it be done without seeming to be jingoistic? Does a new understanding of our nation impact our teaching and study of American art? How do you feel about the idea of a national narrative? Have you been challenged to rethink the relationship between American art, the nation, and its citizenry at any time in the last year?ResponsesDavid M. Lubin, Charlotte C. Weber Professor of Art, Wake Forest UniversityAngela Miller, Professor, Department of Art History and Archaeology, Washington University, Saint LouisPatricia Junker, Ann M. Barwick Curator of American Art, Seattle Art MuseumLauren Lessing, Mirken Director of Academic and Public ProgramsAlan Wallach, Professor Emeritus, Department of Art and Art History, The College of William and MarySally Webster, Professor Emerita, City University of New YorkResearch Notes “Kicked About”: Native Culture at Thomas Jefferson’s MonticelloKristine K. RonanKristine K. Ronan, Independent Scholar Two of the most prominent Native-made objects in Jefferson’s original hall were a pair of male and female figures that Jefferson had received several years prior to Lewis and Clark’s shipments. Curiously, the figures had disappeared from the historical record with Jefferson’s death in 1826. It came as quite a surprise, then, that during my #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-color: #343434; border-left-color: #343434 } #pt-cv-view-6f0799d3kr.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; border-right-color: #343434; border-bottom-color: #343434; } #pt-cv-view-6f0799d3kr .pt-cv-title a, #pt-cv-view-6f0799d3kr .panel-title { font-weight: 600 !important; } #pt-cv-view-6f0799d3kr .pt-cv-title a:hover, #pt-cv-view-6f0799d3kr .panel-title:hover { color: #b53328 !important; } #pt-cv-view-6f0799d3kr .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-6f0799d3kr .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-6f0799d3kr:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-6f0799d3kr .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-6f0799d3kr .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-6f0799d3kr .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-6f0799d3kr .add_to_cart_button, #pt-cv-view-6f0799d3kr .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-6f0799d3kr .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-6f0799d3kr .pt-cv-readmore { color: #ffffff !important; background-color: #2a6ca0 !important; } #pt-cv-view-6f0799d3kr .pt-cv-readmore:hover { color: #ffffff !important; background-color: #000000 !important; } #pt-cv-view-6f0799d3kr + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-6f0799d3kr + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-6f0799d3kr'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-6f0799d3kr'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-6f0799d3kr li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-6f0799d3kr .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-6f0799d3kr .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Book ReviewsConsuming Stories: Kara Walker and the Imagining of American Race Enduring Truths: Sojourner’s Shadows and Substance The American School: Artists and Status in the Late Colonial and Early National Era Building the British Atlantic World: Spaces, Places, and Material Culture, 1600–1850 The Early American Daguerreotype: Cross-Currents in Art and Technology #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-color: #343434; border-left-color: #343434 } #pt-cv-view-4b4916esnz.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; border-right-color: #343434; border-bottom-color: #343434; } #pt-cv-view-4b4916esnz .pt-cv-title a, #pt-cv-view-4b4916esnz .panel-title { font-weight: 600 !important; } #pt-cv-view-4b4916esnz .pt-cv-title a:hover, #pt-cv-view-4b4916esnz .panel-title:hover { color: #b53328 !important; } #pt-cv-view-4b4916esnz .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-4b4916esnz .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-4b4916esnz:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-4b4916esnz .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-4b4916esnz .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-4b4916esnz .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-4b4916esnz .add_to_cart_button, #pt-cv-view-4b4916esnz .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-4b4916esnz .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-4b4916esnz .pt-cv-readmore { color: #ffffff !important; background-color: #2a6ca0 !important; } #pt-cv-view-4b4916esnz .pt-cv-readmore:hover { color: #ffffff !important; background-color: #000000 !important; } #pt-cv-view-4b4916esnz + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-4b4916esnz + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-4b4916esnz'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-4b4916esnz'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-4b4916esnz li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-4b4916esnz .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-4b4916esnz .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Exhibition ReviewsSoul of a Nation: Art in the Age of Black Power The Wall of Respect: Vestiges, Shards and the Legacy of Black Power / Eugene Eda’s Doors for Malcolm X College We Wanted a Revolution: Black Radical Women, 1965–85 Marsden Hartley’s Maine #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-color: #343434; border-left-color: #343434 } #pt-cv-view-66aa710wr8.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; border-right-color: #343434; border-bottom-color: #343434; } #pt-cv-view-66aa710wr8 .pt-cv-title a, #pt-cv-view-66aa710wr8 .panel-title { font-weight: 600 !important; } #pt-cv-view-66aa710wr8 .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-66aa710wr8 .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-66aa710wr8:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-66aa710wr8 .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-66aa710wr8 .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-66aa710wr8 .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-66aa710wr8 .add_to_cart_button, #pt-cv-view-66aa710wr8 .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-66aa710wr8 .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-66aa710wr8 .pt-cv-readmore { color: #ffffff !important; background-color: #2a6ca0 !important; } #pt-cv-view-66aa710wr8 .pt-cv-readmore:hover { color: #ffffff !important; background-color: #000000 !important; } #pt-cv-view-66aa710wr8 + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-66aa710wr8 + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-66aa710wr8'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-66aa710wr8'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-66aa710wr8'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-66aa710wr8'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-66aa710wr8'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-66aa710wr8 li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-66aa710wr8 .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-66aa710wr8 .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Home | Contact Publishing Services | My Account Privacy | Acceptable Use of IT Resources The copyright of these individual works published by the University of Minnesota Libraries Publishing remains with the original creator or editorial team. For uses beyond those covered by law or the Creative Commons license, permission to reuse should be sought directly from the copyright owner listed in the About pages.
This tag should take the reader to The Gustatory Turn as well as the two essays that are being referenced.
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allred720fa18.commons.gc.cuny.edu allred720fa18.commons.gc.cuny.edu
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the consequent prolonged beating about, the past sufferings from obstinate calms, and still continued suffering from thirst; in all these points, as well as others, Don Benito’s story had corroborated not only the wailing ejaculations of the indiscriminate multitude, white and black, but likewise–what seemed impossible to be counterfeit–by the very expression and play of every human feature, which Captain Delano saw.
These "obstinate calms" probably refer to the Intertropical Convergence Zone, or ITCZ. Also know as "the doldrums," in this zone around the equator wind currents of the northern and southern hemisphereshttps://en.wikipedia.org/wiki/Doldrums converge. The doldrums are known for both storms and minimal wind. "Colloquially, the "doldrums" are a state of inactivity, mild depression, listlessness, or stagnation" - which also seems to be the prevailing mood of Benito Cereno, as Capt. Delano perceives him. See also: Page note 1, Tag: "doldrums"

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scholarlykitchen.sspnet.org scholarlykitchen.sspnet.org
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The role of touch in the multi-sensory experience of reading turns out to be as important as we intuit it to be when we hold a volume or turn a page — or better yet, when we mark it up.
I've found that the way I read and my reading retention have changed since I started to regularly use digital annotation. The act of selecting what sentence to highlight, how to tag passages and articles, and what to make public has changed how I feel about reading online. I still prefer paper for pleasure reading, but for news, research, and collaborative reading, digital now works just fine for me.
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editions.lib.umn.edu editions.lib.umn.edu
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Roberta K. Tarbell
The tags for Tarbell and for Ciregna are blank. The tag for Wingate takes us to her essay on Sculpture and Lived Space.
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editions.lib.umn.edu editions.lib.umn.edu
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Dianne Harris
I may not be understanding how tags work, but here again, when I click on Dianne Harris's name, it takes me to a blank tag.
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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We then found the top 2% of predicted nearest neighbors in m-dimensional hash space
For the fly, the data is organized into a higher dimensional space. For LSH, it is organized in a lower dimensional space. But if the algorithms work effectively, the data should still be arranged so that similar features are near one another.
Instead of feature vectors, the algorithms arrange items in hashes or "tags" (just like the fly brain uses a tag to represent an odor, the algorithms use a tag to represent a specific image or word). Finding the hashes that are closest to each other in this new m-dimensional space should reveal the images/words that are most similar to each other (again, if the algorithm works correctly).
These nearby hashes are called "predicted nearest neighbors" because they predict which items in the data set are the most similar.
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sparse
Thinly populated. Here, it means that each tag consists of only a small percentage of the neurons present in the olfactory circuit. That is, only a small percentage of neurons fire action potentials in response to the odor.
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The fly olfactory circuit generates a “tag” for each odor, which is a set of neurons that fire when that odor is presented
In 2015, Stevens identified a three-step code that the fly olfactory circuit uses to identify and respond to specific odors.
- Receptor neurons send odor information to specialized structures called glomeruli, which contain projection neurons.
- Projection neurons send the information to Kenyon cells in the mushroom body.
- These Kenyon cells form the odor label, or tag, and then pass the information along to another stage of neurons that then direct the fly's behavior.
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soundstudiesblog.com soundstudiesblog.com
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I’m sure that after a century of being “the noisiest city on Earth,” folks have gotten creative about it.
Response
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Moreover, if we accounted for the history of zoning in the neighborhoods that have the most or the least complaints it would add another layer of analysis to the data. Are some of these neighborhoods used as entertainment zones, for example? Is it easier to open up bars there than elsewhere in the city?
Condition of Rebuttal/Evidence, depending on POV
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Although it may not be possible to gather who the 311 callers are, including factors such as race and class may lead to very different noise maps. For example, what would a noise map of Manhattan look like if researchers brought income into the equation?
Condition of Rebuttal
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This is where the data falls short. Can it be assumed that those who are calling about the noise are mostly people who live in the neighborhood?
Condition of Rebuttal
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At a glance, loud parties, loud people, and loud car stereos seem to be the major complaints in those areas, according to Sluis’s visualizations
Evidence
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This is key information because it reminds viewers that this neighborhood is a lot more ethnically diverse than other neighborhoods with a smaller number of complaints. It brings to mind: what role does race play in these complaints, in terms of those who complain and those who are the focus of the complaints?
Condition of Rebuttal/New Claim?
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The city may be noisy, but “noisy” is relative. Sluis’s map shows some predictably noisy areas for those of us familiar with Manhattan’s soundscape (Union Square, Times Square) but it also draws attention to other areas not as predictable in the mainstream imagination (East Harlem South, Hamilton Heights).
Condition of Rebuttal
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what stands out is that the major circles of noise complaints are also places where there are different racial and ethnic groups mingling (for example, Times Square) or places that are populated by mostly minorities (Hamilton Heights). Whereas Sluis flattens out the noise complaints, demographic stats point to the racial/ethnic contours of each neighborhood.
Evidence
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Drawn from 2010 census data, the CUNY map clearly delineates neighborhoods and color-codes the groups in each neighborhood per block: blue for whites, green for Latino, orange for black, purple for Asian, and grey for “Other.” Although the Center for Urban Research, CUNY Graduate Center’s maps cannot be superimposed on Sluis’s maps, they help give a general idea as to where neighborhoods are located in addition to racial demographics.
Evidence
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We must remember that annoyance oftentimes stems not just from physical reactions to noise but rather one’s perceptions about noise
Condition of Rebuttal
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40, 412 complaints, to be exact
Evidence
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but neither takes into account the fact that some of the areas with a higher concentration of noise complaints are not just densely populated but densely populated with racial and ethnic minorities
Backing or Qualifier?
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New York City isn’t the only loud city out there
Qualifier
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Although New York City isn’t the only loud city out there, there are many reasons it’s called “The City That Never Sleeps”—and sound has a lot to do with it, depending on which neighborhood you call home.
Claim
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www.nxp.com www.nxp.com
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Grammar [OK] Trademarks [OK] Components [OK] Helper Classes [OK] Analytics [OK] 404s [OK] Code Updates I [OK]
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maildesigner-gangway.s3.amazonaws.com maildesigner-gangway.s3.amazonaws.com
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jave ist endlich da! Und Mail Designer 365 ist natürlich seit dem ersten Tag voll kompatibel zum neuen Betriebssystem.Mit neuen Features, die perfekt auf macOS Mojave abgestimmt sind, gestaltest du jetzt noch effektivere Newsletter, die dein
Bisschen lang oder?
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Share this
Related articles ? Add "coltrane" tag
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www.npr.org www.npr.org
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Hurricane
another test
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en.wikipedia.org en.wikipedia.org
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MLS constitutes one of the major professional sports leagues of the United States and Canada
test
Tags
Annotators
URL
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wikimediafoundation.org wikimediafoundation.org
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of Wikibase for historical research early on.
Wikibase for historical documents
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Rhizome, an arts organization
Wikibase for GLAM
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www.jofreeman.com www.jofreeman.com
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political subcultures
Daniel Elizar asserted that the American political subcultures are traditionalistic, individualistic, and moralistic. Traditionalists support a government that maintains the status quo, individualists see government as a method to further their personal causes, and moralists believe government exists to further the greather good. Source: https://theamericanpartnership.com/tag/elazars-political-culture/
In this context we see that women in the 19th century split to a moralistic subculture.
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ieeexplore.ieee.org ieeexplore.ieee.org
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Memes were provided a categorical tag based on visual content. This tag provided a textual representation of the image even if the image had no embedded textual phrases
Holland's tags~
Tags
Annotators
URL
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github.com github.com
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tools.ietf.org tools.ietf.org
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eskript.ethz.ch eskript.ethz.ch
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Querschnitt
eine Querschnittsstudie ist zB eine Befragung oder ein Experiment das an einem Tag stattfindet.
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claremontdh.net claremontdh.net
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test page note
Tags
Annotators
URL
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www.powazek.com www.powazek.com
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Except that the aggregate selfish behavior of millions of people tagging billions of photos means that the public tag pages make entertaining surfing for everyone.
Reading this reminds me of some of Brad Enslen and Kicks Condor's conversations about discovery on the net.
How can one leverage selfish behaviour to the benefit of all?
Tags
Annotators
URL
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- Sep 2018
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www.thequint.com www.thequint.com
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Import duty on air conditioners, refrigerators and washing machines under 10-kilogram capacity has been doubled to 20 percent each from 10 percent earlier. The basic customs duty on radial tyres is now 15 percent compared with 10 percent earlier,
dch hgchgcvh hkhk
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www.deseretnews.com www.deseretnews.com
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In all, Cassini collected more than 453,000 images and traveled 4.9 billion miles. It was an international endeavor, with 27 nations taking part. The final price tag was $3.9 billion.
The amount of data we have from Cassini is crazy to think about. Quite a few nations were involved in Her mission, and the price tag is relatively low in terms of exploration craft in the past and present.
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www.adamhyde.net www.adamhyde.net
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preparing them for the idea of designing a new system that is native to the web
native to the web

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www.makeuseof.com www.makeuseof.com
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Facebook does not allow third-party apps to display your newsfeed. This applies to Hootsuite. For this reason, you’ll always have to use Facebook natively. The same pretty much goes for Instagram.
Facebook does not allow third-party apps to display your newsfeed. This applies to Hootsuite. For this reason, you’ll always have to use Facebook natively. The same pretty much goes for Instagram.
Tags
Annotators
URL
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www.chromium.org www.chromium.org
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Cross-Origin Read Blocking (CORB) is a new web platform security feature that helps mitigate the threat of side-channel attacks (including Spectre). It is designed to prevent the browser from delivering certain cross-origin network responses to a web page, when they might contain sensitive information and are not needed for existing web features. For example, it will block a cross-origin text/html response requested from a <script> or <img> tag, replacing it with an empty response instead. This is an important part of the protections included with Site Isolation.
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courses.cs.vt.edu courses.cs.vt.edu
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static void f(void) {
defines local symbol f. local to file. without static, g is strong. 3rd line declares but doesnt defifne. right way to do things in c : 1. when you write func implemn/defn, you have 2 choices, you want func glbal enuf. if not global, local then declare fucn declare and declare it as static.
- funcs should be static in filewhenever possible.
you can get away without using header files but you should not do that. every func declaration should be included in header file for a global. implicit func declar. if global func, pople sized, it should have declration in header file but trouble is that lang doesnt require it. cpp requries it therefore its not an extension of c but a diff language. make fucnkign header files. if header files, how does compiler look at functions calls. it looks for dunc declares. you cant overload func names, there is one or none. paramenter list in defn and declare. checks #para and types are compatible. what if compiler doesnt find declaration for func, it makes one up. and assumes return type int always. jeez. cimpiler leaves note for linker, relocation tag, this has to be mapped toa call. that can break down at link time if implicit declareation that compiler made up doesnt match the linker's?. never ignore implicit func declaration warning. PAY ATTENTION TO THESE. main is entry point. lol recursive main?! system calls main. have main call main.
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www.publiconsulting.com www.publiconsulting.com
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famosísima
Ejemplo de anotación: recomiendo visitar esta página web https://disfrutamiruta.com/tag/smorfia-napolitana/
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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we used 20k random projections for the fly to equate the number of mathematical operations used by the fly and LSH
As stated earlier in the article, the authors could only fairly compare the LSH and fly algorithms if each used the same number of mathematical operations.
They determined that if they used 20k random projections in the fly algorithm (where k is the length of the output tag, or hash) then the total number of operations for the fly and LSH algorithms would be equal.
For more detail, see the second paragraph under "Materials and Methods" in the Supplementary Materials document.
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for image search, the tag of an elephant image will be more similar to the tag of another elephant image than to the tag of a skyscraper image.
Common Core State Standards English Language Arts-Literacy, RST 11-12.6: Students should be able to explain why the authors provided this example in the paper, specifically addressing how it adds to the reader's understanding of the research.
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The tag for an odor is computed by a three-step procedure
AP Science Practices, Practice 1: The student can use representations and models to communicate scientific phenomena and solve scientific problems.
Looking at the model in Figure 1A, students should be able to provide a verbal explanation of what the diagram is showing and what each shape and symbol represents.
https://apcentral.collegeboard.org/courses/resources/science-practices
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sparsifying the tag using WTA resulted in better performance than using random tag selection
The authors looked at how the LSH algorithm performed when using two different methods to create the tag:
- The tag is created from a random selection of Kenyon cells
- The tag is created from the Kenyon cells with the highest firing rates (this is the "winner takes all" or WTA approach)
The result was that the LSH performed better with the WTA approach. Note that the authors measured performance using the mean average precision (see Fig. 2B)
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1. C. F. Stevens, Proc. Natl. Acad. Sci. U.S.A. 112, 9460–9465 (2015).
Stevens outlines the three-layer architecture that makes up the fly's olfactory circuit.
He also presents the idea of a unique odor label, or "tag" that is comprised of a small set of neurons and helps the fly identify distinct odors.
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134.76.18.16:8084 134.76.18.16:8084
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First Testtext to pdf embedded via object tag.
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w3c.github.io w3c.github.io
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The toc nav element
This makes it sound like there is an HTML element with a tag name of
<toc nav>rather than<nav epub:type="toc">(which seems to be what's intended).The
landmarksexample farther down is clearer--though the wording there of "the landmark nav element" is equally confusing.There remains only a
navelement, but of varying types.
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- Aug 2018
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www.ed.gov www.ed.gov
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Today, college remains the greatest driver of socioeconomic mobility in America, but if we don't do more to keep it within reach for middle-class families and those striving to get into the middle class, it could have the opposite effect—serving as a barrier, instead of as a ticket to the American Dream.
The American dream has a price tag to rich for all citizens and ergo represents inequality amongst Americans
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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This tag is critical for learning behavioral responses to different odors
Owald and Waddell discovered that the fly olfactory circuit is able to recall previously-learned odors through the help of specialized dopamine neurons. After a fly smells an odor, these dopamine neurons trigger changes in parts of the olfactory circuit that cause the specific neurons associated with the odor (aka the "tag") to fire.
Reactivating the tag causes the fly to remember the odor, as well as the values/meanings/context associated with it. The fly can then exhibit the appropriate behavior based on its prior learning (e.g. avoidance if the odor is associated with danger or approach if the odor is associated with a reward).
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lms.uconn.edu lms.uconn.edu
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Hypothes.is lets you tag
How do you tag the lessons ?
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smadzenuskalosana.wordpress.com smadzenuskalosana.wordpress.com
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tāds kā “grēkāzis” (“ļaunie un maznesaprotošie abortu aizliedzēji”)
Šis cilvēks nesaprot, kā darbojas aktīvisms (un pasaule :D), right? Ak nē, Papardes zieds uzskata, ka aborti nav jāaizliedz un ka abortu aizliegt gribētāji nav jauki cilvēki. Nokrāsojiet mani šokētu! :D
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neviens valstī par abortu aizliegšanu ar likumu nemaz nerunā kā reālu iespēju
Pag, kurā dienā un konkrētā minūtē valstī bija šis maģiskais brīdis? Zinu! Tas notika tad, kad visas planētas sastājās rindā. :D ("Nerunā" neuztvēru 100% burtiski, sorry.)
Un arī nav mūsu valsts vienīgā un pa visu planētu, kā tajā senajā TV reklāmā. :D Polija, Krievija, konservatīvie politiskie spēki, kas atrodami it visur utt? Mm? Tāda sajūta, ka autors dzīvus cilvēkus sen nav saticis un īsti nesaprot, kā tie uztver pasauli.
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Tas arī izsaka šo informatīvo materiālu galveno domu un mērķi. Un, ja pēc tā, kāds uzdrošinātos teikt, ka ir par abortu aizliegšanu, viņš izpelnītos pārmetošus skatienus (kā, tu atbalsti tādas šausmas!).
No way! :D Cepums autoram, ka ir spējis pamanīt šo "slepeno" domu - ka nejaukās sekas no abortu aizliegšanas eksperte uzskata par sliktām (kur pilnībā viņai piekrītu) un negrib pieredzēt tādu nejaucību atkārtošanos.
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Pirmkārt, atlasīti tie eksperti, kas pauž idejai atbalstošu viedokli; svarīgi tas, ka pieredzējuši.
No šit... A ko citu tad Papardes ziedam bija jādara, ņemot vērā šīs organizācijas mērķus? Tajos brīžos, kad tai rodas vēlme apspriest abortus, meklēt nepieredzējušus "ekspertus", kas uzskata, ka aborti ir grēks? :D
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www.digitalethics.org www.digitalethics.org
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This approach, I believe, works well for digital ethics, where we try to articulate rules that govern how we interact with each other through digital technologies. For example, when social media emerged, there was no fixed rule about when it is appropriate to tag someone in a picture and when it isn’t. So we figured out a netiquette and ethical norms as we were going along, based on experience, existing norms, insights from experts etc. There still might be areas of disagreement, but I would argue that overall we have come to an understanding of what is acceptable and what isn’t on this issue, and these norms are passed on to new users of social media.
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www.spiegel.de www.spiegel.de
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if (typeof ADI != 'undefined') ADI.writeAdScript('integrationteaser_1'); Der US-Präsident ist auf Twitter schon wieder zum Gegenangriff übergegangen. Über die "Hexenjagd" gegen ihn selbst und seine früheren Vertrauten empörte sich Donald Trump an diesem Mittwoch einmal mehr. Der Tag zuvor hat ihm zugesetzt, der Schuldspruch gegen seinen Ex-Berate
blabla
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github.com github.com
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There are times when the Hypothes.is browser extension (or the Hypothes.is Via proxy can't "reach" the PDF in a page because it's served in an <iframe> or <embed> tag. This code can be hosted alongside your PDF's to provide the necessary viewer environment and directly embed Hypothes.is, so that it can be used within the <iframe> to annotate the PDF. Simply serve the viewer.html?file= URL's mentioned below via the <iframe src=""> in your CMS, site, or code.
When to use this code
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satsuma.dreamwidth.org satsuma.dreamwidth.org
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dwmeta, future reference, social media
is there a feature hidden somewhere that lets you see all the posts on dreamwidth that use a particular tag? if so, i haven't found it, which definitely makes content discovery difficult
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blogs can be subscribed to through RSS, which will help make the transition off the site easier
I was referencing dreamwidth's syndication feature, which allows you to add anything with an RSS or Atom feed to your reading page. This turned out not to really work - reblogs mean that 95 percent of tumblrs are just way too high volume to work, though certain techniques can mitigate this (a feed for a specific tag on a tumblr blog can be uncovered by adding /rss to the end of the tag URL though automatic feed discovery redirects back to the home feed for some reason)
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developers.google.com developers.google.com
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Split the code into routes and pages
Instead of having a single large bundle file for your whole website, you can have multiple bundles for each page. This improves the load time of your website as you can tag bundle files to the various webpage of your site instead of one initial big download of script file.
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developers.google.com developers.google.com
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providing a fallback to other browsers
For non module supporting browsers we can add another script tag with the nomodule attribute
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mjs
modules have .mjs extension Not really important We can use .js as well The type attribute in our script tag is enough RECOMMENDED : We should use .mjs extension because during development it makes it easier to diffrentiate
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Browsers that understand type="module" ignore scripts with a nomodule attribute.
Only the latest browsers are module-supporting. For these browsers you can use a script tag with a type attribute set to module.
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jats.nlm.nih.gov jats.nlm.nih.gov
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such as DOIs, which might be assigned by, for example, “crossref” or “figshare”
I have to say publicly that this sentence makes absolutely no sense as crossref and figshare are not comparable assigning authorities. CrossRef is a registration agency of the International DOI Foundation; FigShare is not. Based upon the example given, the assigning-authority for a DOI would only ever be the set of agencies that assign DOIs. FigShare is not one of those agencies. See it is not on the list: https://www.doi.org/registration_agencies.html
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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open reading frames (ORFs)
A stretch of DNA sequence that has the ability to be translated into protein (exons only). An ORF usually begins with a start codon (ATG) and ends with a stop codon (TAA, TAG or TGA).
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Yet, under the circumstances, David and his men were not condemned for eating them
Männer mussten rituell rein sein, um das reine Brot zu essen (daher die Frage nach sexuellen Beziehungen => rituell unrein für 1 Tag (3. Mose 15:16))
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- Jul 2018
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speakingjs.com speakingjs.com
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This chapter is about “Basic JavaScript,”
The highlighted annotations with the airbnb tag were made to illustrate where Basic Javascript differs from the Airbnb Javascript Style Guide and typescript tag for differences from Typescript Deep Dive TIPs.
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www.nytimes.com www.nytimes.com
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“I let him know we can’t have this,” Mr. Trump said in an interview with “CBS Evening News.” “We’re not going to have it, and that’s the way it’s going to be.”
asdasd
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astroterf.wordpress.com astroterf.wordpress.com
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If we are now pretending that gay men can and should be induced or somehow ‘educated’ to pursue sexual relationships with people who have vaginas, how is that different from the appalling conversion therapy that used to be forced upon them? If we are now pretending that lesbians can and should be induced or somehow ‘educated’ to pursue sexual relationships with people who have a penis, how is that different from the ‘corrective rape’ forced upon them? It isn’t. Pretending that lesbians and gay men can ‘choose’ to have partners of the opposite sex is coercive heterosexuality and it’s regressive, reactionary nonsense.
https://via.hypothes.is/https://gendertrender.wordpress.com/tag/corrective-rape/
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www.kickscondor.com www.kickscondor.com
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Likes, upvotes, replies, friending. What if it’s all just linking? In fact, what if linking is actually more meaningful!
This is sort of the fun, I think, in maintaining things like listen and read posts on my site. While they're a useful archive for me, in some part I hope they might speed some discovery for folks who find them or search them by category/tag as well.
I could post somewhere, "Hey I listen to this podcast," or retweet a headline, but invariably in the morass of content out there, there isn't actually an indication that I invested my finite amount of time actually listening to or reading that thing. Perhaps I was just doing some social signaling to make myself seem more interesting or worldly? To me this is a lot of the value of these types of posts.
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localhost:8000 localhost:8000
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In conceptual terms, the new LINSIGHT method is closely related to our previous fitCons method
- annotation test 1;
- annotation 2;
Tags
Annotators
URL
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www.postgresql.org www.postgresql.org
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A document is the unit of searching in a full text search system; for example, a magazine article or email message.
Test.
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course-computational-literary-analysis.netlify.com course-computational-literary-analysis.netlify.com
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She says what I have done so far isn’t in the least what I was wanted to do. I am asked to tell the story of the Diamond and, instead of that, I have been telling the story of my own self
Because the narrative jumps around, it would be interesting to map out each event chronologically and to tag it as to how it relates to the moonstone. Perhaps some kind of network / chronology?
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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On 2017 Jun 14, Youhe Gao commented:
I found two "overexpression"s in the paper. "Although the extent of bait overexpression is difficult to judge and varies across IP's, previous experimentation has shown that over-expression has little effect on identification of true interacting partners (Sowa et al., 2009)" "VAPBWT overexpression strongly increased the association of EGFP-LSG1 and OSBP with the ER (Figure 7E,G)" Personally, I am not sure if those are enough. In a system, increasing [A] or [B] will lead to more [AB]. As we know more about protein interaction now, this kind of systematic false positive should not be ignored any more. In cells, overexpression with tag may even change the location of the protein. That is why I think the next generation of massive protein interaction studies should start from in vivo crosslinking. I do not want to overemphasize the problem. Most of the protein interactions identified are probably true in cells. The amount of work done is very impressive and respected. I hope users who is using a particular interaction data as the only clue for their future experiment design, maybe they should start with an in vivo crosslinking as a conformation of that interaction. It may make them more confident to proceed.
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europepmc.org europepmc.org
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On 2017 Jan 22, Eric Fauman commented:
I know nothing about cow genetics, but I have done some work on the genetics of metabolites in humans, so I was interested to see how the authors derived biological insights from this genetic study. In particular, I was intrigued by the suggestion in the abstract that they found evidence that genes involved in the synthesis of “milk components” are important for lactation persistence.
Unfortunately, the more I studied the paper the more problems I found that call this claim into question.
First off, the Q-Q plot is currently unavailable, but the text mentions there’s only a “slight deviation in the upper right tail”, which could mean there are no true significant signals.
To account for multiple testing, the authors decided to use a genome-wide association p-value cutoff of 0.95/44100 = 2.15e-5 instead of a more defensible 0.05/44100 = 1.1e-6.
Since their initial p-value cutoff yielded a relatively small number of significant SNPs, the authors used a much more lenient p-value cutoff of 5e-4 which presumably is well within the linear portion of the Q-Q plot.
The biggest problem with the enrichment analysis, however, is that they’ve neglected to account for genes drawn from a common locus. Often, paralogs of similar function are proximal in the genome. But typically we assume that a single SNP is affecting the function of only a single gene at a locus. So, for example, a SNP near the APOA4/APOA1/APOC3/APOA5 locus can tag all 4 genes, but it’s unfair to consider that 4 independent indications that “phospholipid efflux”, “reverse cholesterol transport”, “triglyceride homeostasis” and other pathways are “enriched” in this GWAS.
This issue, of overcounting pathways due to gene duplication, affects all their top findings, presumably rendering them non-significant. Besides lipid pathways, this issue also pertains to the “lactation” GO term, which was selected based on the genes GC, HK2, CSN2 and CSN3. GC, CSN2 and CSN3 are all co-located on Chromosome 6.
A perplexing claim in the paper is for the enrichment of the term “lipid metabolic process” (GO:0006629). According to the Ensembl Biomart, 912 Bos taurus genes fall into this category, or about 4% of the bovine protein coding genes (24616 according to Ensembl). So out of their set of 536 genes (flanking SNPs with P < 5e-4) we’d expect about 20 “lipid metabolic process” genes. And yet, this paper reports only 7. This might be significant, but for depletion, not enrichment.
Sample size is of course a huge issue in GWAS. While 3,800 cows is a large number, it appears this trait may require a substantially larger number of animals before it can yield biologically meaningful results.
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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On 2016 Sep 16, Hilda Bastian commented:
There are many important issues raised in this paper on which I strongly agree with John Ioannidis. There is a lot of research waste in meta-analyses and systematic reviews, and a flood of very low quality, and he points out the contributing factors clearly. However, there are some issues to be aware of in considering the analyses in this paper on the growth of these papers, and their growth in comparison with randomized and other clinical trials.
Although the author refers to PubMed's "tag" for systematic reviews, there is no tagging process for systematic reviews, as there is for meta-analyses and trials. Although "systematic review" is available as a choice under "article types", that option is a filtered search using Clinical Queries (PubMed Help), not a tagging of publication type. Comparing filtered results to tagged results is not comparing like with like in 2 critical ways.
Firstly, the proportion of non-systematic reviews in the filter is far higher than the proportion of non-meta-analyses and non-trials in the tagged results. And secondly, full tagging of publication types for MEDLINE/PubMed takes considerable time. When considering a recent year, the gulf between filtered and tagged results widens. For example, as of December 2015 when Ioannidis' searches were done, the tag identified 9,135 meta-analyses. Today (15 September 2016), the same search identifies 11,263. For the type randomized controlled trial, the number tagged increased from 23,133 in December to 29,118 today.
In the absence of tagging for systematic reviews, the more appropriate comparisons are using filters for both systematic reviews and trials as the base for trends, especially for a year as recent as 2014. Using the Clinical Queries filter for both systematic reviews and therapy trials (broad), for example, shows 34,126 for systematic reviews and 250,195 trials. Page and colleagues estimate there were perhaps 8,000 actual systematic reviews according to a fairly stringent definition (Page MJ, 2016) and the Centre for Reviews and Dissemination added just short of 9,000 systematic reviews to its database in 2014 (PubMed Health). So far, the Cochrane Collaboration has around 38,000 trials in its trials register for 2014 (searching on the word trial in CENTRAL externally).
The number of systematic reviews/meta-analyses has increased greatly, but not as dramatically as this paper's comparisons suggest, and the data do not tend to support the conclusion in the abstract here that "Currently, probably more systematic reviews of trials than new randomized trials are published annually".
Ioannidis suggests some bases for some reasonable duplication of systematic reviews - these are descriptive studies, with many subjective choices along the way. However, there is another critical reason that is not raised: the need for updates. This can be by the same group publishing a new version of a systematic review or by others. In areas with substantial questions and considerable ongoing research, multiple reviews are needed.
I strongly agree with the concerns raised about conflicted systematic reviews. In addition to the issues of manufacturer conflicts, it is important not to underestimate the extent of other kinds of bias (see for example my comment here). Realistically, though, conflicted reviews will continue, building in a need for additional reviewers to tackle the same ground.
Systematic reviews have found important homes in clinical practice guidelines, health technology assessment, and reimbursement decision-making for both public and private health insurance. But underuse of high quality systematic reviews remains a more significant problem than is addressed here. Even when a systematic review does not identify a strong basis in favor of one option or another, that can still be valuable for decision making - especially in the face of conflicted claims of superiority (and wishful thinking). However, systematic reviews are still not being used enough - especially in shaping subsequent research (see for example Habre C, 2014).
I agree with Ioannidis that collaborations working prospectively to keep a body of evidence up-to-date is an important direction to go - and it is encouraging that the living cumulative network meta-analysis has arrived (Créquit P, 2016). That direction was also highlighted in Page and Moher's accompanying editorial (Page MJ, 2016). However, I'm not so sure how much of a solution this is going to be. The experience of the Cochrane Collaboration suggests this is even harder than it seems. And consider how excited people were back in 1995 at the groundbreaking publication of the protocol for prospective, collaborative meta-analysis of statin trials (Anonymous, 1995) - and the continuing controversy that swirls, tornado-like, around it today (Godlee, 2016).
We need higher standards, and skills in critiquing the claims of systematic reviews and meta-analyses need to spread. Meta-analysis factories are a serious problem. But I still think the most critical issues we face are making systematic reviews quicker and more efficient to do, and to use good ones more effectively and thoroughly than we do now (Chalmers I, 2009, Tsafnat G, 2014).
Disclosure: I work on projects related to systematic reviews at the NCBI (National Center for Biotechnology Information, U.S. National Library of Medicine), including some aspects that relate to the inclusion of systematic reviews in PubMed. I co-authored a paper related to issues raised here several years ago (Bastian H, 2010), and was one of the founding members of the Cochrane Collaboration.
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europepmc.org europepmc.org
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On 2016 Jun 02, Michael Tatham commented:
Is SUMO5 a pseudogene?
There are known to be many SUMO pseudogenes in humans (http://www.ncbi.nlm.nih.gov/pubmed/12383504). A fair position when confronted with a claim that a new SUMO paralog has been discovered is to assume it is a non-expressed pseudogene until otherwise convincing evidence is provided. This paper lacks one piece of critical evidence supporting the idea that SUMO5 really exists as a protein, and that is the presence of endogenous protein.
When BLAST searched, the nucleotide sequence of SUMO5 (originally termed SUMO13 according to the authors’ GenBank entry: FJ042790.1), returns a top hit of “Homo sapiens SUMO1 pseudogene 1 (SUMO1P1), non-coding RNA Sequence ID: ref|NR_002189.3|”. The only difference is a single nucleotide T23 (in SUMO13/SUMO5), which is C in SUMO1P1. This may be a primer synthesis error or a DNA sequencing error.
To put beyond reasonable doubt that SUMO5 is not a pseudogene at least two pieces of new experimental evidence showing SUMO5 is expressed in cells is required. I can think of three good ways to do this:
(1) Mass-spectrometric evidence of a peptide unique to SUMO5.
(2) Cross-reaction of a SUMO5-specific antibody with an endogenous protein.
(3) Editing of the genome to insert an epitope tag into the endogenous SUMO5 gene, with the intention of detecting the protein using an antibody specific to the tag.
All three of these pieces of evidence will be strengthened by parallel studies comparing cells with and without SUMO5-specific knock-down.
RTPCR experiments intending to detect mRNA are particularly uninformative given that DNA contamination often leads to false-positives. This is especially true for SUMO5 given the fact the gene is intronless, a notable characteristic of pseudogenes.
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europepmc.org europepmc.org
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On 2016 Jul 27, Duke RNA Biology Journal Club commented:
This is a summary of a journal club discussion:
This is one of four articles using similar imaging techniques to study translation in living cells published at the same time. These publications add to the growing number of techniques used to image translation such as mature fluorescent proteins Yu J, 2006, TRICK Halstead JM, 2015, and RNA-binding protein/mRNA co-fluorescence Wu B, 2015. The technique presented in this article is similar to the last except that it uses Suntag to image the nascent chain and PP7 aptamers on the mRNA. The colocalization of the two represents active translation in polysomes.
The technique is novel because co-localization is detected as the protein is translated. This brings fluorescent V4-peptide antibodies into concentrated foci at a single point, and can thus be used to follow multiple rounds of translation. Because of this, only detection of the translated protein is needed and indeed, past the first figure, the mRNA fluorescence is not shown. Fast changes in translation can be detected as shown using the ATF4 ORF construct translational response to stress shown in Figure 4 with the possibility of extending the time of tracking to hours by anchoring the mRNA Yan X, 2016 or using fast 3D imaging techniques. One unusual observation the authors made was the vast heterogeneity of transcript translation within a single cell; at any given time only a subset of the transcripts undergo translation and translation rates may vary depending on as yet unknown factors. A related observation is the diffusion of polysomes within the cell: polysomes translating cytosolic transcripts have slower diffusion rates in the perinuclear region of the cell compared to the cytoplasm. This could be due to the restrictive architecture of a membranous area but the exact mechanism remains unknown. A second surprising observation indicates mRNAs that have begun translation and are associated with polysomes can be transported in dendrites, contrary to earlier reports Besse F, 2008. However, the authors cannot detect if translation is temporarily stalled during transport.
While this technique makes substantial findings in the area of single transcript translation behavior, there are limitations. All in all, these images are dots that respond to translation inhibitors, meaning the resolution is not good enough to detect codon resolution and should be coupled with other techniques to verify observations and determine their mechanism. Additionally, since detection of the nascent chain wouldn’t be detected until the majority of the V4 peptides were translated, initiation would be overlooked; however, TRICK is an existing technique for studying the first round of translation.Our main criticism with this technique is the extensive construct engineering that must be performed which raises concerns over disturbing the mRNA and protein functions from both the PP7 aptamers, the Suntag peptides and an ornithine decarboxylase tag to facilitate rapid degradation of the protein. These engineering steps add over 2 kb to the original gene. Additionally, an antibody against the Suntag and a fluorescent PP7 coat protein must be expressed in the cytosol. While the constructs studied did not cause harm to the cell, each construct of interest must be tested individually. Along this line, while there is the possibility to multiplex by changing the aptamer loop or peptide-antibody combination, it would be difficult to multiplex above two individual transcripts. Thus large-scale studies involving individual translation dynamics of mRNA subsets would remain time consuming and technically challenging.
A quick comparison with the three other papers show agreements among all of them Iwasaki S, 2016 however, there is a great opportunity to learn by reading the papers to compare experimental approaches of three groups. We look forward to see what novel findings this technique uncovers as it becomes adopted in different laboratories.
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europepmc.org europepmc.org
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On 2016 Jun 21, Evelina Tutucci commented:
We have also recently discussed Nelles et al. Nelles DA, 2016. Since we are interested in developing new techniques for studying gene expression and mRNA localization at the single molecule level, a potential tag-less system to detect mRNAs in fixed and live cells would be a further advance. As pointed out by the Duke RNA Biology journal club we think that Nelles et al. represents an attempt to apply the Cas9 System to detect endogenous mRNA molecules. Unfortunately, no evidence is presented to demonstrate that this system is ready to be used to study gene expression at the single molecule level, as the MS2-MCP system allows. The RNA letter by Garcia and Parker Garcia JF, 2015 showed that in S. cerevisiae the binding of the MS2 coat protein to the MS2-loops diminished tagged mRNA degradation by the cytoplasmic exonuclease Xrn1. However, these observations were not extended to higher eukaryotes. Previous work from our lab described the generation of the beta-actin-MS2 mouse, whereby all the endogenous beta-actin mRNAs were tagged with 24 MS2 loops in the 3’UTR (Lionnet T, 2011, Park HY, 2014). This mouse is viable and no phenotypic defects are observed. In addition, control experiments were performed to show that the co-expression of the MS2 coat protein in the beta-actin-MS2 mouse allowed correct mRNA degradation and expression (Supplementary figure 1b, Lionnet T. et al 2011). Furthermore, multi-color FISH (Supplementary figure 6, Lionnet T. et al 2011) showed substantial co-localization between the ORF FISH probes and MS2 FISH probes, demonstrating the validity of this model. We think that the observations by Garcia and Parker are restricted to yeast because of the short half-life of their mRNAs, wherein the degradation of the MS2 becomes rate-limiting. Based on our extensive use of the MS2-MCP system, we think that higher eukaryotes may have more time to degrade the high affinity complexes formed between MS2-MCP, providing validation for this system to study multiple aspects of gene expression. In conclusion, we think that the MS2-MCP system remains to date the best method to follow mRNAs at the single molecule level in living cells. For the use of the MS2-MCP system in S. cerevisiae we have taken the necessary steps to improve it for the study of rapidly degrading mRNAs and are preparing this work for publication.<br> Evelina Tutucci and Maria Vera, Singerlab
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