- Apr 2020
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wissen.hypotheses.org wissen.hypotheses.org
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Bref, certains journalistes pratiquent à une large échelle le « bâtonnage de dépêche » , de sorte que celui-ci occupe une part importante de leur temps : ils sélectionnent, éditent et publient des dépêches d’agence de presse, à un rythme soutenu ; ils publient sur le site web des articles écrits pour le journal papier. Dans tous les cas, ils ne vont pas (ou très peu) sur le terrain, et ils ne produisent des textes originaux qu’à la marge, quand ils ont le temps.
Joue sur la pénibilité du travail accompli et la notion d'usinage dans les tâches pour accentuer le coté harassant ("sélectionnent, éditent, publient.... rythme soutenu...quand ils ont le temps" )
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Il faut évidemment prendre quelques précautions. Ainsi, en l’état actuel des recherches, il est impossible d’affirmer que ces manuels et mémoires sont représentatifs du journalisme francophone dans son ensemble, ni même qu’ils reflètent une pratique réellement intense ou au contraire plus marginale
hypothèse plus que louable sur le fait que le journalisme n'est surement pas représentatif d'un plagiat constant. Mais qu'il est sûrement "l'arbre qui cache la forêt" et qu'il faut le voir dans son ensemble et sa globalité .
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on voit des copieurs-colleurs rendre honnêtement hommage aux créateurs originaux (si cette notion existe encore) des contenus qu’ils répliquent. D’autres, au contraire, se les approprient sans vergogne, et sans même un lien hypertexte pour signaler la source.
Met en évidence le côté répréhensible et sans honte du journalisme plagiat.
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Et pourtant, les ciseaux et la colle jouent un rôle non négligeable dans les processus de production de l’information journalistique. Ou en tout cas, leurs jumeaux métaphoriques qui peuplent toutes nos interfaces informatiques : le copier/coller, CTRL-C/CTRL-V.
"de l'information journalistique" généralisation sur un certain journalisme, sans toutefois représenter une majorité .
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Annotators
URL
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- Mar 2020
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theconversation.com theconversation.com
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Trouvez du soutien dans cette démarche qui va à contre-courant de la tendance générale de surconsommation et de surconnection. Non, vous n’êtes pas seul. Oui, il existe d’autres manières de vivre. Vous pouvez prendre part à des activités de groupe vous permettant de vous recentrer sur vos sens et votre ressenti. Par exemple, apprendre à jouer d’un instrument de musique, à sculpter le bois, à jardiner – même si vous habitez en ville, etc.
L’enchaînement des idées a un effet persuasif au service de l'auteure. Fluide, "non, vous n'êtes pas seul. Oui, il existe d'autres manière de vivre." encourage le lecteur tout en défendant le point de vue de l'auteure.
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Est-ce que vous perdez la notion du temps quand vous surfez le Web ? Vous ne pouvez pas vous empêcher de regarder votre smartphone lorsqu’il vibre ? Vous paniquez si vous oubliez votre téléphone à la maison ? Vous êtes peut-être accro au digital.
L'auteure accroche le lecteur par des questions fermées où le lecteur peut s'identifier. Il en découle une phrase "vous êtes peut-être accro au digital" incitant à continuer la lecture.
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Ce scénario vous paraît familier ? Selon une étude menée par Microsoft, la capacité de concentration de l’homme est passée de 12 à 8 secondes en dix ans. La cause ? L’omniprésence des écrans. Une étude de l’université de Californie à Irvine montre que travailler en étant constamment interrompu augmente le niveau de stress, car on a tendance à travailler plus vite pour rattraper le temps perdu. Aujourd’hui, une personne sur quatre vérifie son smartphone toutes les 30 minutes et 25 % des Millennials le consultent plus de cent fois par jour.
L'auteure cite des études scientifiques avec les liens de celle-ci pour argumenter sa première partie. Elle enchaîne et présente les différents études sans réellement les mettre en lien entre elles. On peut considérer qu'il s'agit d'un raisonnement épistémique de type inductif où les faits vérifiés (de l'étude) permettent d'élaborer une conclusion générale émise juste après. On peut néanmoins mettre en avant le raccourci que fait l'auteure en citant ces différentes études une par une pour finir par poser sa conclusion, regroupant les conclusions des différentes études sans lien apparent entre elles.
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Je dois écrire un article scientifique suite à une conférence sur le réchauffement climatique. Il est temps que je m’y mette. Je m’installe à mon ordinateur. Document Word créé. Études scientifiques sélectionnées. Pile de livres à ma droite. Tisane d’ortie à ma gauche. J’ai une heure devant moi pour travailler sur cet article. Le multitasking, vous connaissez ? Dessin de l’auteur Fatalement, je suis connectée à Internet pour vérifier mes sources et peaufiner mon argumentaire. Fatalement, je reçois un ou cinq e-mails auxquels je ne réponds pas mais qui me déconcentrent. Mon téléphone vibre, mon ordinateur affiche des notifications, ma tablette m’envoie des annonces. Afin de gérer ce pic inattendu de cortisol, je tente de me calmer en regardant des photos sur Instagram, une vidéo sur YouTube et quelques posts sur un blog. Au final, j’ai perdu 20 minutes.
Dans un premier temps, l'auteure utilise un raisonnement rhétorique de type pathos pour susciter des émotions chez le lecteur afin d'argumenter sa première partie. Original, cette partie permet de faire adhérer le lecteur à son premier argument: "passer du temps en ligne affecte notre productivité".
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- Jan 2020
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www.sciencedirect.com www.sciencedirect.com
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Arachnodactyly
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001166
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Joint laxity
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001388
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Slender limbs
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001533
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Scoliosis
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0002650
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Tall stature
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000098
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aortic root dilatation
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0002616
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mitral regurgitation
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001653
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mitral valve prolapse
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001634
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large ears
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000400
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Midface hypoplasia
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0011800
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Open mouth appearance
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000194
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Short philtrum
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000322
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Long face
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000276
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Hypotonia
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001290
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aggressive behavior
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000718
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intellectual disability
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001249
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Developmental delay
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001263
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allele id lookup result: https://reg.clinicalgenome.org/redmine/projects/registry/genboree_registry/by_caid?caid=CA414193300
Tags
- HP:0000276
- individual:4
- ClinGen
- HP:0001166
- HP:0000098
- HP:0002650
- allele:CA414193300
- hpoLookup
- HP:0001249
- HP:0000322
- HP:0001634
- gene:NKAP
- alleleLookup
- alleleLookup:individual
- HP:0000194
- hpoLookup:individual
- monarchLookup
- HP:0001388
- HP:0002616
- HP:0001653
- HP:0001290
- HP:0001263
- HP:0011800
- HP:0001533
- HP:0000400
- HP:0000718
Annotators
URL
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-GENO-190322-4
DOI: 10.7554/eLife.47061
Resource: (ZFIN Cat# ZDB-GENO-190322-4,RRID:ZFIN_ZDB-GENO-190322-4)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-GENO-190322-4
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www.sciencedirect.com www.sciencedirect.com
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RRID:IMSR_OBS:4
DOI: 10.1016/j.celrep.2019.07.078
Resource: (IMSR Cat# OBS_4,RRID:IMSR_OBS:4)
Curator: @ethanbadger
SciCrunch record: RRID:IMSR_OBS:4
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-150424-4
DOI: 10.7554/eLife.42455
Resource: (ZFIN Cat# ZDB-ALT-150424-4,RRID:ZFIN_ZDB-ALT-150424-4)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-150424-4
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-060821-4
DOI: 10.7554/eLife.42881
Resource: (ZFIN Cat# ZDB-ALT-060821-4,RRID:ZFIN_ZDB-ALT-060821-4)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-060821-4
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reg.clinicalgenome.org reg.clinicalgenome.org
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allele id lookup result: http://reg.clinicalgenome.org/redmine/projects/registry/genboree_registry/by_caid?caid=CA399193709
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www.sciencedirect.com www.sciencedirect.com
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4
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0002415
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seizures
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001250
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intellectual disability
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001249
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developmental delay
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001263
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www.sciencedirect.com www.sciencedirect.com
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variant id lookup result: https://www.ncbi.nlm.nih.gov/clinvar/variation/692269/
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variant id lookup result: https://www.ncbi.nlm.nih.gov/clinvar/variation/692269/
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- Dec 2019
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www.sciencedirect.com www.sciencedirect.com
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failure to thrive
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001508
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- Nov 2019
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www.pcmag.com www.pcmag.com
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a promising technology for decades that's never truly caught on. That's constantly changing with the current wave of VR products,
PC magazine is a online compuer magazine, based on popular topics ranging form hackers to smartphones.
Rating: 9/10
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- Sep 2019
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portablesink.com portablesink.com
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Are you looking for portable sink manufacturer to get portable 4 compartment sink? At MONSAM Portable Sinks, they offer four-compartment portable self-contained sinks which are perfect for use as a commercial portable sink.
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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LC-MS Analysis
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- Aug 2019
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Lumbar gibbus
Monarch lookup result: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294674/#annotations:NNq7xL0dEemwJUvA1NrCBA
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disproportionate stature
Monarch lookup result: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294674/#annotations:NNq7xL0dEemwJUvA1NrCBA
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platyspondyly
Monarch lookup result: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294674/#annotations:NNq7xL0dEemwJUvA1NrCBA
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- Jul 2019
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www.milestonedocuments.com www.milestonedocuments.com
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Eventually, the term Communist became identified with the brand of Socialism that came to be called “Marxism.”
Is this the same Communism as the Soviet Union Communism?
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Annotators
URL
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www.milestonedocuments.com www.milestonedocuments.com
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to my Celestial Court and to be in control of your country’s trade with China, this request is contrary to all usage of my dynasty and cannot possibly be entertained. It is true that Europeans, in the service of the dynasty, have been permitted to live at Peking, but they are compelled to adopt Chinese dress, they are strictly confined to their own precincts and are never permitted to return home.
The emperor basically saying that diplomat sent to his country cannot return home
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You, O King, live beyond the confines of many seas, nevertheless, impelled by your humble desire to partake of the benefits of our civilisation, you have dispatched a mission respectfully bearing your memorial. Your Envoy has crossed the seas and paid his respects at my Court on the anniversary of my birthday. To show your devotion, you have also sent offerings of your country’s produce.
throughout the entire passage the emperor had a condescending tone
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Annotators
URL
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www.milestonedocuments.com www.milestonedocuments.com
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All those people in China who sell opium or smoke opium should receive the death penalty.
The selling of opium is punishable by death
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Annotators
URL
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Preparation of distilled extracts
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Conclusion
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Preparation of distilled extracts
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Tests for Saponins
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Preparation of distilled extracts
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Qualitative and quantitative estimation of DNA
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Sporulation medium used for Trichodermasp
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Collection of the diseased materia
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Relative prevalence of extracellular virulence factors among Vibriofrom Cochin estuary, shrimp farm and seafood
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Detection of type III secretion system genes
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Production of gelatinase
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Plasmid profiles among Vibriofrom Cochin estuary, shrimp farms and seafood
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Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood
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Plasmid profiling of the drug resistant strains
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Detection ofblaNDM-1gene
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Antibiotic resistance among Vibriofrom seafood
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Seasonal variation in the diversity and distribution of Vibrioin Cochin estuary
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Gel documentation and image analysis
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Indole production
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Isolation of Vibriospecies from water and sediment of Cochin estuary
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Indole production
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Isolation of Vibriospecies from water and sediment of Cochin estuary
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sg.inflibnet.ac.in sg.inflibnet.ac.inChapter 415
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Active site identification, metal detection and interaction of Dof domain structure
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Superposition of the Dof domain with predicted 3D structure
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Validation of the predicted 3D structure
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Tertiary structural prediction
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Secondary structural prediction
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Secondary and tertiary structure prediction of SbDof proteins o
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Gene structure prediction
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Phylogenetic and motif analysis of sequenced Dof domains
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In silico characterization of sequenced Dof domains of cereal
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Sequencing of Dof domain and gene
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Cloning of Dof genes of sorghum using pBSK vector
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Cloning of Dof domain and Dof genes using pGEM-T Easy
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Gel elution of PCR products
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PCR based cloning, sequencing and in silico characterization of Dof domain and Dofgenes of cereals and millet
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Comparative analysis of cereals and millets based on banding patterns generated by Dof domain and Dof genes-specific primers
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)
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Analysis of PCR amplicons using agarose gel electrophoresis
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Qualitative analysis of DNA by agarose gel electrophoresi
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Plasmids and bacterial strain
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Estimation of antibody response in rLdADHT+BCG vaccinated hamsters
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Statistical analysis
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Post-challenge survival
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Determination of antileishmanial antibody responses in hamster
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RT - PCR
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cDNA synthesis and amplification
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Formaldehyde gel electrophoresis
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RNA isolation
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Quantification of mRNA cytokines and inducible NO synthase (iNOS) in hamsters by Real time-PCR
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NO production
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LTT assay
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DTH
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Immunological assays
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Patients and isolation of peripheral blood mononuclear cells (PB
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Purification of recombinant ADHT (rLdADHT)
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Subcloning of ADHT in pET-28a expression vector
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Sequencing of ADHT gene
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Cloning of gene in pTZ57R/T (T/A cloning) vecto
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Results of Python Molecular Viewer
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Molecular Docking
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Catalase assay
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Detection of intracellular ROS
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Nitric oxide radical quenching activity
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DLS particle size and zeta potential analysis
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Synthesis of AgNPs
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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The Posterior Probability of Mixing Dis-tribution
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Spreading inhibitory behavior
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Inhibition of haemocytes spreading behavior
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Oral toxicity bioassay
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Influence of prey on VS yield
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Quantification of VS for protein
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Salivary gland-Morphology and Anatomy
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Gross morphology and histology of salivary gland
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Fish sampled from selected water bodies for further analysis
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Increments in biomass:
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- Jun 2019
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krishikosh.egranth.ac.in krishikosh.egranth.ac.in
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Organic carbon
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Soil pH and electrical conductivity (EC)
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Mechanical composition
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Methods of soil analyses
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krishikosh.egranth.ac.in krishikosh.egranth.ac.in
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Trait plasticity and response to competition
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Root length (cm) and Root weight (mg)
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Number of productive tillers per meter
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Concentration of ideal carbon source
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Carbon sources
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NaCl concentration
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Temperature
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pH
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Static and shaken condition
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Inoculum concentration
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Optimization
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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IFN γ production by CD8 T cells upon stimulation with PMA and viral peptides
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Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns
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Phenotypic and Functional Characterization of CD8 T cells in cord blood
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CD107a expression (marker of cytotoxicity)
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Serological and Virological Assessment
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Lysis buffer for Ni-NTA and glutathione purification
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Luciferase reporter assay
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General reagents and plastic wares
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GFP-β-catenin- C (634 -781 a.a)
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Analysis of ROS
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Plasmid
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Tissue Culture Medium
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.inThesis8
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Standard
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Calculation
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Procedure
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Reagent
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Proline
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Fresh and Dry weight
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Root and Shoot length
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Morphological Studies
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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ELISA binding Buffer
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Preparation of the vaccines
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Large scale preparation of plasmid DNA
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Ultra-competent cells
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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RS-1 Assay
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RS-1 Assay
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Chorioallantoic membrane (CAM) assay
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Procedur
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Reagents
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Hydroxyl radical scavenging assay
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Electrochemical Measurement
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Single Cell Tes
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Electrochemical Measurement
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Physical Characterizatio
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Catalyst Preparation
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Material
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STRUCTURAL STABILITY AND ELECTROCHEMICAL PERFORMANCE OF W-DOPED PLATINUM ALLOY NANO PARTICLES FOR SEAWATERELECTROLYSIS ON ALCOHOL OXIDATION INNON-MEMBRANE FUEL CELL SYSTEMS
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Electrochemical Measuremen
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Electrochemical Measuremen
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Total alkalinity
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Characterization of embelin isolated from E.ribes
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High Performance Liquid Chromatography (HPLC) analysis
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Foaming Index
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Estimation of free aminoacids
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Per cent Overlap:
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Somatotype Categories:
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Mean Somatotypes:
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Urban-Rural Somatotype Comparisons
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somatotype Categories:
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Somatotype Categories:
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Mesomorph-endomorph:
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engl201.opened.ca engl201.opened.ca
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¶PQRWVD\LQJRQHJUDSKLVEHWWHUWKDQWKHRWKHU,QIDFWWKH\FDQFRPSOHPHQWHDFKRWKHU+RZ\RXVSOLW\RXUGDWDGHSHQGVRQKRZPXFKGHWDLO\RXQHHGRUGRQ¶WQHHG
Figure 1-6 and 1-7 look very different, and it could be hard to see that they are documenting the same set of data. This manipulation of data and how it is viewd can be used for very specific intents. If I wanted a client to see that my business has a consistent viewer rate, I would show them them figure 1-7's monthly chart, because of how smooth it looks. To me at first glance, it seems much more "consistent" than figure 1-6, with all of its spikes.
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This is also an important part of marketing. While there is a specific "thing" or "data" that is being produced, the facts are not necessarily all that you're looking for. You want to look at how this connects to the readers or buyers, what it makes them feel and think. Ultimately, the goal is to make these people buy into the thing/idea etc. being sold, .
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7KHIRUPHUUHDGVDVDQXPEHUZLWKRXWPXFKFRQWH[WZKHUHDVWKHODWWHULVPRUHUHODWDEOH
I think the whole idea of visualizations and statistics is to create something relateable. Without providing context there would be no way for the general public to interact with the data without intense reading and critical analysis. And, let's be honest, who really wants to do that much thinking about every little thing? Creating visualizations help us all to understand complex ideas easier.
It also helps us to visualize these complex data sets to see patterns and themes that could have otherwise been overlooked. Not only do these visualizations help the general public to understand, it also helps professionals in their field analyze data within different contexts to see all of the potential.
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)LQDOO\DOZD\VFRQVLGHU\RXUDXGLHQFHDQGWKHSXUSRVHRI\RXUJUDSKLFV
I believe this is very important. I think a lot of people tend to forget who they are presenting to, and lots of the valuable information goes to waste. In m Health Care Systems class we were always presented with lots of statistical data but nothing was every explain nor was the source the data was collected from presented so it seemed very misleading. Lots of numbers were thrown around without a true explanation given. After reading through this article, it seems more information was needed for both the students and the professor to understand the information provided.
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This reminds me of Organic Chemistry labs. When data points don't make sense, something must have gone wrong in the experiment. It was vital to ensure that the source was found to determine if it would effect the results or if the experiment needed to be redone.
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%XEEOHVUHSUHVHQWFRXQWULHVDQGPRYHEDVHGRQWKHFRUUHVSRQGLQJFRXQWU\¶VSRYHUW\GXULQJDJLYHQ\HDU
I wonder why bubbles were used instead of a geographical map displaying the percent? I know it mentions how it puts people to sleep, but wouldn't this be more confusing?
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7KHLQWHUDFWLYHSLHFHVFUDSHVVHQWHQFHVDQGSKUDVHVIURPSHUVRQDOSXEOLFEORJVDQGWKHQYLVXDOL]HVWKHPDVDER[RIIORDWLQJEXEEOHV
This seems extremely difficult to follow. I'm wondering how people who are colour blind might interpret this chart due to the similar colours used to display the stats.
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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The complementary segments of a globin needed for the semisynthesis of mutant chains were prepared by V8 protease digestion (Sivaram eta/, 2001). The a globin was dissolved in 0.01 M ammonium acetate buffer (pH 4) at a concentration of 1.0 mg/ml and digested at 37°C with V8 protease (1: 200, w/w) for 3 hours. The completion of digestion was ascertained by RPHPLC, after which the reaction was quenched by addition of neat TFA to a final concentration of 0.1 %. The complementary segments, al-30 and a31-141, from the digestion mixture were isolated in pure form by size-exclusion chromatography on a Sephadex G50 column (98cm x 2.8cm). The column was equilibrated and run in 0.1% TFA. The lyophilized sample of the digest was dissolved in the above solvent and loaded on to the column. The column was run at a flow rate of 30 mllhour and the elution profile monitored at 280 nm. The individual chromatographic profile of a globin digest showed only two peaks, a31-141 and a1-30 respectively, as expected from a single cleavage at the 30-31 peptide bond. The peak fractions were pooled separately and lyophilized
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Generation of complementary fragments, al-30 and a31-141, from heme-free a globin
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- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Xylanolytic activity was determined according to Archana and Satyanarayana (1997). The reaction mixture containing 0.5 mL of 1% birchwood xylan in glycine NaOH buffer (0.1 M, pH 9.0) and 0.5 mL of cell free sonicated supernatant was incubated at 80 °C in a water bath for 10 min. After incubation, 1 mL DNSA reagent (Miller, 1959) was added to the reaction mixture and the tubes were incubated in a boiling water bath for 10 min, followed by the addition of 400 μL of 33% w/v sodium potassium tartrate. The absorbance values were recorded at 540 nm in a spectrophotometer (Shimadzu, Japan). The liberated reducing sugars were determined by comparing the absorbance values of these with a standard curve drawn with different concentrations of xylose. One unit (IU) of xylanase is defined as the amount of enzyme required for liberating one μmol of reducing sugar as xylose mL-1 min-1under the assay conditions. Composition of Dinitrosalicylic acid (DNSA) reagent NaOH - 10.0 g Phenol - 2.0 g DNSA - 2.0 g Distilled Water - 1000 mL DNSA reagent was stored in an amber bottle at 4 °C till further use. Sodium sulphite (0.05 % v/v) was added just before the use of the reagent.
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