4,254 Matching Annotations
  1. Nov 2020
    1. Image-based memes involve, primarily, an image created by somebody. Sometimes the meme creator is also the image creator, but often, when involving movie stills or images of celebrities, the image’s copyright is owned by someone else. American copyright law gives creators the exclusive rights of reproduction, modification, distribution, performance, and display. The viral spread of a meme infringes on theses protections as the original image is modified and then displayed, distributed and reproduced when posted and reposted.

      Memes are basically just ways of making fun of certain pictures, a lot of the time, they happen to be real people caught at a weird or funny moment and the catchy tag you put on the picture is what makes it funny.

    1. None

      I am surprised to see no honorable mention here, because a "book log" sounds a lot like a reference manager. The best free/open-source one I know of is Zotero: https://www.zotero.org

      From your list of desired features above, it can do:

      • tagging of items (automatically when collecting items with the browser button, manually, or a mix of both automatic tags and your own tags)
      • notes as attachments to an item
      • bookmarks as an URL attached to an item (and actually, most item types collected with the browser button have the URL saved by default)
      • making items and their annotations public on your profile on zotero.org
      • shareable format: you can export in many formats, from simple printout kind of formats (HTML) to formats fully re-importable into another instance of Zotero
      • query: not sure it has all the capabilities of a relational database, but you can search based on any piece of metadata found in your items, you can build arbitrarily complex search queries, you can save searches (they will materialize in the interface as "dynamic folders" containing the search results automatically as new items added to your library match the query)

      For dealing with prioritization, you would have to come up with your own system. The workflow described here uses the tag system for this (with custom tags to mark status "to read", "read", etc.): https://incenp.org/notes/2019/managing-academic-literature.html

    2. Allow me to tag books instead of placing them into static lists (think clusters or tag clouds).

      Have your tried Roam Research?

    1. Mouse monoclonal anti-V5

      DOI: 10.1016/j.cub.2020.10.061

      Resource: (Thermo Fisher Scientific Cat# R960-25, RRID:AB_2556564)

      Curator: @Naa003

      SciCrunch record: RRID:AB_2556564

      Curator comments: V5 Tag Monoclonal Antibody Thermo Fisher Scientific Cat# R960-25


      What is this?

    1. We’re now 100% powered by renewable and sustainable energy which is great in further minimizing our impact on the planet. Plausible Analytics script weights less than 1 KB which is more than 45 times smaller than the recommended Google Analytics Global Site Tag implementation.

      After speaking to the folks at Plausible they pointed me to this page on the digital ocean community forums:

      https://www.digitalocean.com/community/questions/what-kind-of-electricity-do-you-run-on

      And this one here:

      https://www.interxion.com/why-interxion/sustainability

      The TLDR version is that the servers they are using are run by Digital Ocean, who lease from Interxion, who source the power for the datacentre from renewables.

      Interxion themselves are owned by Digital Realty, who do release figures, but not at a granularity to confirm.

      Once there is info from Interxion, it's possible to confirm this.

    1. 500 iPad-Koffer mit insgesamt 8000 Geräten

      Ich bin ein großer iPad-Fan und nutze meines jeden Tag für Handschriftliches.

      Dieser Aktion ist bestimmt eine gründliche Evaluierung der Optionen vorausgegangen und es ist toll, dass unsere Schulen jetzt besser ausgestattet werden, gar keine Frage.

      Trotzdem nagt die Erkenntnis an mir, dass ein iPad doch in erster Linie ein Konsum- und Kommunikationsgerät und weniger ein Kreativwerkzeug ist. Ich frage mich deshalb, ob die iPads nicht zumindest um günstige Laptops mit Tastatur ergänzt werden sollten.

      Ich schreibe diesen Kommentar übrigens gerade auf einem RaspberryPi für 100€. Davon bekommt man so etwa vier Stück für den Preis eines iPads. Und unglaublich viel mehr Möglichkeiten.

    1. Anthony Tattersal

      I suggest to add a link to his biography or any other link that tells the reader who he is

    1. Benardou, Agiatis, Panos Constantopoulos, Costis Dallas, et Dimitris Gavrilis. 2010. « Understanding the information requirements of arts and humanities scholarship ». International Journal of Digital Curation 5 (1):18‑33. « British Museum Collection ». 2015. https://old.datahub.io/dataset/british-museum-collection. Brown, Susan. 2011. « Don’t Mind the Gap: Evolving Digital Modes of Scholarly Production across the Digital-Humanities Divide ». In Retooling the humanities: The culture of research in Canadian universities, édité par Daniel Coleman et Smaro Kamboureli, 203‑31. Edmonton: University of Alberta Press. http://hdl.handle.net/10402/era.25382. Brown, Susan, et John Simpson. 2013. « The curious identity of Michael Field and its implications for humanities research with the semantic web ». In 2013 IEEE International Conference on Big Data, 77‑85. IEEE. http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6691674&tag=1. Bulger, M, E Meyer, G De la Flor, M Terras, S Wyatt, M Jirotka, K Eccles, et others. 2011. « Reinventing research? Information practices in the humanities ». Information Practices in the Humanities (March 2011). A Research Information Network Report. Crane, Gregory. 2006. « What do you do with a million books? » D-Lib magazine 12 (3). Corporation for National Research Initiatives. « DBpedia ». 2015. https://wiki.dbpedia.org/. « Digital Environmental Humanities ». 2015. https://dig-eh.org/. « Dublin Core Metada Initiative ». 2015. https://www.dublincore.org/. Egerton, Frank N. 2013. « History of ecological sciences, part 47: Ernst Haeckel’s ecology ». The Bulletin of the Ecological Society of America 94 (3). JSTOR:222‑44. « eMOP: Early Modern OCR Project ». 2015. https://emop.tamu.edu/. Europeana. 2014. « Linked Open Data ». Europeana Pro. https://pro.europeana.eu/page/linked-open-data. Fons, Ted. 2014. « Transforming bibliographic records into linked open data (LOD) ». Panel presentation at the Coalition for Networked Information Fall 2014. https://www.cni.org/topics/information-access-retrieval/exposing-library-collections-on-the-web-challenges-and-lessons-learned. Godby, Jean, Karen Smith-Yoshimura, Bruce Washburn, Kalan Knudson Davis, Karen Detling, Christine Fernsebner Eslao, Steven Folsom, et al. 2019. « Creating Library Linked Data with Wikibase: Lessons Learned from Project Passage ». OCLC Research Report. https://www.oclc.org/content/dam/research/publications/2019/oclcresearch-creating-library-linked-data-with-wikibase-project-passage.pdf. Hegde, Medha. 2012. « Ecotones: the transitional zones ». Biotech Articles, nᵒ 12. http://www.biotecharticles.com/Biology-Article/Ecotones-The-Transitional-Zones-2191.html. Hendler, Jim, et others. 2011. « Why the Semantic Web will never work ». In 7th Extended Semantic Web Conference (ESWC 2011), Crete, Greece. http://videolectures.net/eswc2011_hendler_work/. Internet Philosophy Ontology (InPhO) Project. s. d. « The InPhO Project ». Consulté le 19 juin 2020. https://www.inphoproject.org/. Jaeger, Paul T, Jimmy Lin, Justin M Grimes, et Shannon N Simmons. 2009. « Where is the cloud? Geography, economics, environment, and jurisdiction in cloud computing ». First Monday 14 (5). Klein, Max. 2012. « VIAFbot Debriefing ». OCLC Research. https://hangingtogether.org/?p=2306. Krafft, Dean, et Tom Cramer. 2014. « Video: Linked Data For Libraries (LD4L) Project Update ». Coalition for Networked Information. https://www.cni.org/news/video-linked-data-for-libraries-ld4l-project-update. Lam, Dominic. 2014. « Big Data Challenges in Social Sciences & Humanities Research ». Datanami. https://www.datanami.com/2014/09/08/big-data-challenges-social-sciences-humanities-research/. « Linked Data for Libraries (LD4L) ». 2014. https://wiki.lyrasis.org/pages/viewpage.action?pageId=41354028. LODE: Linked Open Data Enhancer. s. d. « Gihub Linkedhumanities/lode ». Consulté le 19 juin 2020. https://github.com/linkedhumanities/lode. McCarty, William. 2005. Humanities Computing. Palgrave Macmillan UK. Nardi, Bonnie, et Vicki O’Day. 1999. « Information Ecologies: Using Technology with Heart-Chapter Four ». First Monday 4 (5). Valauskas, Edward J. http://firstmonday.org/ojs/index.php/fm/article/view/672/582. OCLC Research. 2014. « Scholars’ Contributions to VIAF ». https://www.oclc.org/research/areas/data-science/viaf-scholars.html. « Open Annotation Data Model ». 2013. http://www.openannotation.org/spec/core/. Pan-Canadian Documentary Heritage Network. s. d. « Linked Open Data (LOD) Visualization “Proof-of-Concept.” ». Canadiana. Consulté le 13 septembre 2015. http://www.canadiana.ca/sites/pub.canadiana.ca/files/PCDHN\%20Proof-of-concept\_Final-Report-ENG\_0.pdf. Price, Gary. 2012. « Video: “Out of the Trenches: A Linked Open Data Project” From the Pan-Canadian Documentary Heritage Network ». LJ infoDOCKET. https://www.infodocket.com/2012/10/25/video-out-of-the-trenches-a-linked-open-data-project-from-pan-canadian-documentary-heritage-network/. Risser, Paul G. 1990. « The ecological importance of land-water ecotones ». In The ecology and management of aquatic-terrestrial ecotones, édité par H Décamps et Naiman R J, 7‑21. Paris: UNESCO. « Schema.org ». 2015. https://schema.org/. Searle, John R. 1995. The construction of social reality. New York: Simon; Schuster. Simpson, John Edward, Susan Brown, et Lisa Goddard. 2013. « A Humanist Perspective on Building Ontologies in Theory and Practice. » In Digital Humanities Conference Abstracts 2013, édité par University of Nebraska, 403‑5. Lincoln. http://dh2013.unl.edu/abstracts/ab-413.html. Smith-Yoshimura, Karen, David Michelson, et Beth Mardutho. 2013. « Irreconcilable differences? Name authority control & humanities scholarship ». OCLC Research. http://hangingtogether.org/?p=2621. « The Muninn Project ». 2015. http://blog.muninn-project.org/. The Stanford Natural Language Processing Group. s. d. « Software > Stanford Named Entity Recognizer (NER) ». Consulté le 19 juin 2020. https://nlp.stanford.edu/software/CRF-NER.html. Uddin, Mueen, et Azizah Abdul Rahman. 2011. « Techniques to implement in green data centres to achieve energy efficiency and reduce global warming effects ». International Journal of Global Warming 3 (4). Inderscience Publishers:372‑89. « VIAF ». 2015. https://viaf.org/. « VIVO Open Research Networking Community Group ». 2015. https://www.w3.org/community/vivo/. Warren, Robert. 2012. « Creating specialized ontologies using Wikipedia: The Muninn experience ». Proceedings of Wikipedia Academy: Research and Free Knowledge (WPAC2012). Berlin. https://wikipedia-academy.wikimedia.de/w/images.wikipedia-academy-2012/0/0f/21_Paper_Robert_Warren.pdf. Widmer, Rolf, Heidi Oswald-Krapf, Deepali Sinha-Khetriwal, Max Schnellmann, et Heinz Böni. 2005. « Global perspectives on e-waste ». Environmental impact assessment review 25 (5). Elsevier:436‑58. « WorldCat Entities ». 2015. OCLC Developer Network. https://www.oclc.org/developer/develop/linked-data/worldcat-entities.en.html. Wuppleman, William. 2012. « Out of the trenches: A linked open data project ». Canadiana. https://www.canadiana.ca.

      Pour la bibliographie issue d'internet, il faut uniformiser dans un sens où dans l'autre : certains sites portent la mention "Consulté le", d'autres non.

    1. A Wikipedia kifogása az iTA szócikkel kapcsolatban

      Mint Wikipedia szerkesztésért "felelős" jelzem: ne törődj ezzel! Nem számít túlzottan, ha majd az "supervisor"-ok mégis jeleznek (amit nekem fognak), teszek valamit. A tag törölhető - szerintem.

    1. (15x) ENJOYMENT: Forgettable Outstanding(10x) DEPTH (IN RELATION TO COMPLEXITY): Lacking Meaty (5x) LUCK FACTOR: All Luck All Skill (3x) REPLAYABILITY: Nil Limitless(10x) MECHANICS: Boring Interesting (4x) PLAYER INTERACTION: Low High (4x) PLAYER COUNT PERFORMANCE: Not Balanced Balanced (2x) GAME LENGTH: Too Short/Long Just Right (2x) CLARITY OF RULES: Mud Crystal (5x) COMPONENT QUALITY: Cheap World ClassINITIAL RATING (sum(Criteria Rating x Criteria Weight)/Total Weight) = 7.7

      rating scale evaluation

    1. mouse monoclonal anti-myc

      DOI: 10.1016/j.ajhg.2020.10.012

      Resource: (Millipore Cat# 05-724, RRID:AB_309938)

      Curator: @Naa003

      SciCrunch record: RRID:AB_309938

      Curator comments: Anti-Myc Tag, clone 4A6 antibody Millipore Cat# 05-724


      What is this?

    1. The FBI said it has stopped using the "Black Identity Extremist" tag and acknowledged that white supremacist violence is the biggest terrorist threat this country faces.

      Look at her face, its kinda the face like oh you guys are finally noticing this. I think it's really good that they are noticing these things and working to stop it. It's really good that people are still talking about this because if they dont I feel that some may start to forget.

    1. Luckily, Tinder offers a variety of additional signal amplifiers that help you to stand out. The sole purpose of features like Tinder Boost and Super Likes is to outcompete status rivals by giving you preferential signaling treatment. And guess what – they come with a price tag.

      Julian claims Tinder is monetizing on signal amplifiers like Boost and Super Like.

    2. Another point of evidence is the lack of luxury software products. People spend absurd amounts of money on jewellery, handbags and cars, but I can’t think of a piece of software with an even remotely similar price tag. Sure, people have tried to sell $999 apps but those never took off.

      Julian Lehr posits that because software purchases are less visible, their signalling power is reduced. This is why, for instance, you don't see any luxury software products: Because you cannot signal you're in on it.

    1. It isn't really compatible with HTML5's input "required" attribute. If an input has the required tag, and you press the submit buton, and the field is empty the browser will fire the "Please fill out this field" message, BUT, you also just disabled that submit button. So in effect, the form can no longer be submitted.
    1. This whole system is much, much better than having to manually update some CRM like in Airtable. Since you're naturally tagging people as you interact with them, you can create an easy record of your relationship with them and compile any useful notes on them as you go.

      If you use Roam as a CRM, in your daily note you can simply tag a person you just had a meeting with and log some notes. Those notes will then show up under that person in the linked references under a block for the current date.

      So in one sentence, with using only your keyboard, you've created a meeting note linked to a person and linked to a specific date.

      With any other solution you'd have to navigate to a person, create an entry, set a date and write the note.

      This "decide where to put it" step is completely replaced with "what entities does this pertain to".

    2. This removes all the decision making about where to put things that you frequently run into with Evernote, Notion, etc. When everything can be everywhere, you don’t have to worry about the filing structure. You just keep adding links. 

      Nat's conclusion is correct, but his reason for arriving at that conclusion is wrong.

      You're not faced with the question of where to put things with Roam because you can do the following:

      (1) You can tag a new entry on the fly, in-line, CLI style. (2) If the tag exists, it will autocomplete, if it doesn't you can create it with no extra effort (3) Any tags you add are links to their respective pages, which allows you to (a) navigate their as soon as you've typed the tag/page name and (b) it creates a backlink on those pages so your new entry is automatically linked to from there.

    3. By structuring information in this way, Roam makes it super easy to move laterally across your information, while retaining vertical references. The book Emergency by Neil Strauss can live in my Book Notes page, my Prepping page, and my Neil Strauss page, without having to be moved. 

      I think Nat touches on an important use case here, but I wouldn't call it "moving laterally while retaining vertical references."

      He's referring to a link to the book Emergency, not some content of the book itself. So each page can link to the book, that's not novel.

      What is novel is that when entering in the book into your Roam database you can tag it with Prepping and Neil Strauss and it will show up under those pages automatically.

    4. This also highlights a big difference between Roam and other note taking tools: tags are both everything and nothing. Every page is a tag, and every tag is a page.

      Nat says that tags are everything and nothing, but I don't agree with that.

      Pages consist of blocks.

      A reference to a page is treated in the exact same way as a tag.

      A block is not treated in the same way. A block is not a tag.

    1. By keeping the price of ebooks high, publishers keep paperbacks as a valid option for readers. That way, the world of physical books isn't under threat of becoming extinct due to ebooks.

      Do you like this reason?

    2. If you're an avid reader, you may know the pain of losing or damaging your books. Ebooks, however, don't share this problem.

      How long do ebooks last?

      Has one of your ebooks ever become damaged?

      Have you ever lost an ebook?

    3. On top of this, ebooks are very convenient for the readers buying them. Buying a physical book involves going to a bookstore and hoping they have it in stock, or ordering it online and waiting for it to arrive. For ebooks, you go to a website, click the "Buy" button, and download the book to your PC or reader.

      Are people willing to pay for convenience?

    4. This constraint is the reason ebooks sometimes cost more than paperbacks. For example, a publisher can list the price of their physical book at $27.95 and the ebook at $20, which is a reasonable 30 percent markdown.

      Explain why ebooks sometimes cost more than physical books.

    5. Unlike with physical books, Amazon has no control over the price of ebooks. If someone has performed the steps required to publish an ebook via Kindle Direct Publishing, they set the price as they please, with no exceptions.

      Do you think this is true for authors who don't have a following?

    6. However, ebooks utilize the agency model when sold. Instead of letting the retailer choose the price, the publisher states what they're selling for. The publisher gets 70 percent of each transaction, and the retailer gets the remaining 30 percent.

      How is the pricing system for ebooks different from the one for physical books?

    7. Everything makes sense when you imagine all of the people who helped make the book who need paying. For one, the author has to get their agreed royalty cut from every sale. From there, the editors, proofreaders, cover artists, and marketers all need to be paid. These obligations don't leave the publisher with a lot of money for themselves.

      Who else needs to be paid besides just the author?

    8. a physical book takes around $1-2 to produce. If this is true, however, then why are they priced a lot more than that?

      Does this surprise you?

    1. Great list! Some things we didn't think about until we needed them are:

      • stroller organizer
      • teethers
      • crinkly books
      • pacifier holders but ones that could probably hold other things too like teethers or wubba nubs
      • baby sunglasses
      • Milkies trays - you'll want 1oz milk cubes for putting in the boon silicone thing when she's teething and in the beginning you'll bag and freeze less oz so as not to waste but then she'll grow and need just one oz more so you can defrost 1oz at a time.
      • If you are going to try to breastfeed have emergency formula on hand in case it doesn't go well or an emergency where Rob will have to feed. These single packets are great because you can put some in the diaper bag for just in case & I found out that once you open a tin of formula you must use w/in 30 days so if it's just for emergencies the tin is a waste.
      • A very Extra purchase but we LOVE it: a baby cam for the car instead of the stupid mirrors that really don't work--it also has night vision so you can see them at night whereas you can't see them with a mirror. We have the Yada and love it but it looks like there are now some cheaper ones that are highly reviewed.
      • diaper caddy so you can change diapers in any room
      • these washable portable changing pads-one in the caddy, one in the car, one in your diaper bag
      • reusable swim diaper
      • a brush, we obviously knew our kid would have hair, maybe a toss up with your kid
      • this is the baby sunscreen we got thinkbaby) & Babo
      • a giant play mat to roll out and away
      • spray oxy clean or the powder to soak all the dirty stuff
      • if you are up for (evidence-based, because I'm a researcher nerd) pregnancy and parenting book recs I LOVE Emily Oster
      • a foot stool for your glider while nursing
      • socks, are the worst so we love the booties with snaps: zutano
      • Baby tylenol
      • Baby saline drops
      • baby vitamin D drops
      • a giant water bottle (insulated if you prefer cold water) with a STRAW -- you don't have two hands ever again to unscrew a top and you'll be thirsty all the time while breastfeeding
      • a nightstand next to your glider stocked with more water and granola bars, protein bars, (or in my case, poptarts). You'll be so hungry at 2am
      • BLACKOUT curtains! These travel ones are great because you can put them up wherever for naps.
      • a giant basket to hold toys/books/blankets
    1. Oh, and from a language/design perspective, you can actually turn regular words in a sentence into channels, just as many people do with @replies. For example: I’m coming to #barcamp later today.

      Because the use of hashtags is inline and you can turn regular words into hashtags (and therefor channels), there is no friction to do so.

    2. It also enforces actual use in the wild of tags, since no evidence of a tag will exist without it first being used in conversation. This means that representing channels in tagclouds across the site that grow and fade over time, and are contextual to all of Twitter or to a single user, is the ideal interface for displaying this information.

      Hashtags have the added benefit that they won't show up for others if they're not used.

      If you look at which hashtags are being used (trending), you get a taxonomy of micro-contexts, ranked by popularity, with which you can navigate Twitter. All from the bottom up.

    3. I also like that the folksonomic approach (as in, there are no “pre-established groups”) allows for a great deal of expression, of negotiation (I imagine that #barcamp will be a common tag between events, but that’s fine, since if there is a collision, say between two separate BarCamps on the same day, they’ll just have to socially engineer a solution and probably pick a new tag, like #barcampblock) and of decay (that is, over time, as tags are used less frequently, other people can reuse them — no domain squatting!).

      The folksonomic approach (user-generated tagging) is beneficial because it allows complexity to emerge bottom-up.

    4. Every time someone uses a channel tag to mark a status, not only do we know something specific about that status, but others can eavesdrop on the context of it and then join in the channel and contribute as well. Rather than trying to ping-pong discussion between one or more individuals with daisy-chained @replies, using a simple #reply means that people not in the @reply queue will be able to follow along, as people do with Flickr or Delicious tags. Furthermore, topics that enter into existing channels will become visible to those who have previously joined in the discussion. And, perhaps best of all, anyone can choose to leave or remove topics that don’t interest them.

      Twitter's hashtags form a dual purpose. They label a status with a certain tag, telling us something about the intended context of that Tweet.

      The ease of which makes it frictionless for anyone to jump into the conversation.

      But they also equip an interested eavesdropper with the ability to follow along with a conversation. This idea (at the time this was being discussed at Twitter) was already happening with Flickr and Delicious tags.

    5. This is how it works in IRC, and how it needed to work in Twitter.

      The idea of:

      When you use a hastag and the channel with that name doesn't exist, it gets created, is an idea that came from IRC.

    6. Now, in thinking about implementing channels, it was imperative that I not introduce any significant changes into the way that I currently use Twitter any more than I have for other features that have been added to Twitter (for example, @replies or direct messages). Channels would need to be a command-line-friendly addition, and one that would require absolutely zero web-based management to make the most of it (to draw a distinction, Pownce fails this test with its Friend Sets, since it requires use of their website to take advantage of this feature).

      The requirements [[Joe Messina]] laid out for a concept of "channels" on Twitter was that:

      1. It shouldn't add any friction to his current use
      2. It shouldn't require any web-based management to make the most of

      Twitter of 2020 satisfies these requirements. You just type #something, and you can click on that hash or search for it to see results.

    7. Jaiku comes closest with their channels implementation, making it extremely easy to create new channels (simply post a message that begins with a hash (#) and your intended channel name — and if the channel doesn’t exist, it’ll be created for you):

      [[Joe Messina]] details an example from [[Jaiku]] where you can create a channel by simply posting a message that starts with a hash (#). If the channel doesn't exist, it will be created for you.

    8. I’m more interested in simply having a better eavesdropping experience on Twitter.

      [[Joe Messina]]'s reason for suggesting the hashtag was his interest in having "better eavesdropping experience on Twitter"

    1. SGML

      Az SGML (Standard Generalized Markup Language, szabványos általános jelölőnyelv) egy ISO szabványos jelölőnyelv dokumentumformátumok leírására. Az SGML elődjét, a GML-t (Generalized Markup Language) az 1960-as években fejlesztette ki az IBM-nél Charles Goldfarb, Edward Mosher és Raymond Lorie (családnevük kezdőbetűi alapján találta ki Goldfarb a GML nevet). Ennek leszármazottja az SGML, ami 1986-ban lett ISO ( International Organization for Standardization) szabvány.[1]

      Az SGML egy absztrakt szintaxist biztosít, amit sokféle alkalmazásban használhatunk. A szabványos szintaktika lehetővé teszi, hogy az ilyen formátumú dokumentumokat egy általános célú értelmezővel (parser) könnyen beolvashassuk, írhassuk vagy formailag ellenőrizhessük. SGML-ben a jelölések (tag) jelentése nincs meghatározva, ez mindig az SGML-t használó alkalmazás feladata (például a HTML-ben, ami az egyik legismertebb SGML alkalmazás, a jelöléseknek már konkrét jelentésük van, és a jelölések értékkészlete véges).

    1. anti-HA

      DOI: 10.1016/j.celrep.2020.108332

      Resource: (Cell Signaling Technology Cat# 3724, RRID:AB_1549585)

      Curator: @Naa003

      SciCrunch record: RRID:AB_1549585

      Curator comments: Rabbit Anti-HA-Tag Monoclonal Antibody, Unconjugated, Clone C29F4 Cell Signaling Technology Cat# 3724


      What is this?

    1. Schulprogramm (wird überarbeitet)

      Muss vor dem Tag der offenen Tür auf jeden Fall da hin ;-). (Note to myself)

    1. It looks like you just deleted our lovely crafted issue template. It was there for good reasons. Please help us solving your issue by answering the questions asked in this template. I'm closing this. Please either update the issue with the template and reopen, or open a new issue.

      Ignoring official advice

    1. group: Ariel Methodology Group Narrow your search: user: search by username tag: search for annotations with a tag url: search by URLfor domain level search add trailing /* eg. example.com/* group: show annotations associated with a group Danfff1

      test note

    1. And that’s because to treat graphic design like it’s a service, where it makes sense to optimize time and labor for maximum efficiency, undermines the aura of indispensability, superiority, and yes, authenticity that institutions such as design schools and “professional associations” rely on in order to justify the massive dollar signs they place on themselves via tuition, and member fees.

      I'm assuming Libby means that Rob Giampietro is on the other side of the argument where viewing design as something to be optimized for efficiency undermines that high dollar price tag that institutions have marketed as only possible by studying with them. But she is on the side that, that thinking is irrelevant to how design ought to progress.

    1. http://jonudell.info/h/tag-rename-02.mp4

      Most people would embed a YouTube video. Nice to see no dependency on 3rd-party service here.

    1. The submission system required students to manually enter all ‘‘tags’’ (rele-vant topic keywords) for their letters, entering up to five tags per letter; thesystem did not provide a menu list of common issues for students to choosefrom.

      I like that the tags were student generated. Personally, when given a list to choose a tag from sometimes I don't feel the tag is listed that is most appropriate. I enjoy that students were able to create their own tags to summarize the main issue of their letter.

      For example, Samuel H. chose a "classroom" tag that peaked my interest. I was curious to explore what issues in the classroom he believed should be addressed. This is one of the aspects that stood out to me.

  2. Oct 2020
    1. Looking at all those bearing, heading, orientation, navigation, position, direction, etc. I think we have a bigger problem here. Someone has decided how to use tag (e.g. orientation is about page orientation), but there are 100 other cases. Imho, to disallow misusing there should be no "heading", but rather "html-heading", "gps-heading", "whatelse-heading", which make mistakes impossible. So yes, "heading" should go.
    2. Retagging the HTML/CSS questions to use html-heading seems the right thing to do. For the other uses, I don't have enough grounding in the geographic area to know whether the direction and bearing are replacements for heading. But the tag information for heading should be created and should firmly point at the other tags — at least until it is expunged.
    1. Wurde beispielsweise ein Algorithmus zur Erkennung von Hautkrankheiten an Bildern isländischer Patienten trainiert, wäre das eine wichtige Information, da der Algorithmus womöglich bei australischen Ureinwohnern eine völlige andere Trefferquote an den Tag legt.

      Welche Rolle spielt das Training von Algorithmen? Wie funktioniert es?

    1. Reviewer #1:

      H3K14ub is a histone modification that facilitates deposition of H3K9me on heterochromatin in fission yeast, but the mechanism by which this modification stimulates Clr4 was unknown. Using mutants and HDX, the authors identified the interaction surface of Clr4 for H3K14ub, which they used to design mutants that responded poorly to H3K14ub stimulation. In vivo, these mutations resulted in loss of heterochromatin marks and defects in heterochromatin-based silencing, suggesting that H3K14ub stimulation is essential to K9me-mediated silencing. Finally, the authors show that human SUV39H2 but not G9a or Arabidopsis SUVH4 can be stimulated by H3K14ub in a similar manner.

      The authors provided biochemical and structural insights into the mechanism that increases the H3K9-specific methyltransferase activity of Clr4 by H3K14ub. Although H3K14ub-mediated promotion of H3K9 methylation is shown in Oya et al. EMBO Rep 2019, this study further characterizes the potential mechanism. However, there are some issues with the results that need to be resolved.

      1) Similarity and difference with the previous study. As the authors acknowledge, this manuscript builds on a previous study by Oya et al. 2019, however I think the similarities and the differences need to be made even more explicit and better addressed.

      a) The authors should clearly state that Figure 1B and 1C are basically a confirmation of Oya et al. 2019.

      b) I am more puzzled by the difference in the mapping of the region required for H3K14ub stimulation. The authors suggest that a difference in the preparation of the recombinant proteins might be responsible. This can and should be tested as it would seemingly be a simple experiment (compare with and without GST tag).

      c) Possibly to reconcile their findings with the previous report the authors state in the description of Fig. 1 that "the N-terminus plays a regulatory role in the sensing of H3K14ub by the catalytic domain" but I don't see this reflected in the data show in Fig. 1C, given that the degree of stimulation is very similar for KMT and FL.

      2) Stimulation-defective mutants. The authors should carefully discuss the stimulation-defective mutants, which should be premised on the retention of their methyltransferase activity on unmodified H3. The authors claim that 30% loss of activity of the Clr4 KMT mutants on unmodified H3 is observed in Figure S3C (Pg 11 line 15), but this cannot be determined from the graph provided, which is normalized to unmodified H3. The authors should (1) make another graph to show the 30% loss and (2) compare Clr4 KMT mutants with catalytic-dead Clr4 KMT or dissolution buffer (no protein). It is still possible that GS253 and F3A mutations simply reduce MTase activity, thus displaying lower activity than WT in the presence of H3K14ub, which would also suggest a different interpretation for the results in vivo.

      3) Heterochromatin localization of Clr4 mutants. The FLAG ChIP results in Fig. 4E is not very informative, as with the loss of heterochromatin a loss of Clr4 is predicted. If the authors want to test whether the localization activity of Clr4 mutants is intact, (1) FLAG ChIP in the clr4+, Flag-Clr4GS253/F3A background (i.e., two clr4 alleles exist) or (2) in vitro H3K9me2/3 binding assay should be performed. Since Clr4 N-terminus might regulate MTase activity as discussed in Pg 18 line 19, it is also possible that amino acid substitutions in the KMT region affect the function of N-terminus, including CD. The co-IP in Fig. 4C is not sufficient to clarify this point as Clr4 directly binds heterochromatin via its CD, in addition to the CLRC-mediated mechanism, and it is unclear if this is affected in the mutants.

      4) Allosteric vs. binding regulation. On Pg. 11, the authors suggest that an allosteric mechanism is at play, but this is not supported by the data. In fact the observation that providing ubiquitin in trans does not stimulate and rather inhibits the activity on H3K14ub would suggest that the ubiquitin just increases binding affinity. To clarify this the authors should measure binding affinity of WT and mutants to the H3 peptide with and without ubiquitin.

    1. Reviewer #2:

      This manuscript further characterizes the role of HILPDA/HIG2 in TAG/LD biology. The major finding is that HILPDA interacts with and promotes DGAT activity and TAG synthesis, which is novel given that HILPDA has largely been thought to regulate TAG turnover as a lipolytic inhibitor.

      Characterization of the interaction between HILPDA and DGAT1 (and to a lesser extent DGAT2) is the major strength of this paper and an important advancement in the field. The early parts of the paper are not particularly novel (Fig. 1) or well-designed (Fig 2. - poor NAFLD/NASH model showing almost no effects) and the study is a bit on the thin side for data.

      1) The data shown in Figure 1 is not particularly striking given that HILPDA is a known target gene of PPAR-alpha, which is activated by FAs. Showing that HILPDA expression tracks with PLIN2 is also pretty obvious as PLIN2 tracks with LD accumulation. I really don't see the need/relevance of this figure.

      2) The MCD diet is widely regarded as a poor model for NAFLD/NASH since it doesn't replicate human NASH in so many regards. As a result, the use of this model makes these studies less relevant. Also, it is referenced that HILPDA was found to be up in a MCD study, but why not look at the plethora of human and mouse studies of NAFLD that have done RNAseq or arrays to provide a more physiological assessment of its expression in NAFLD/NASH?

      3) The conclusion that effects are independent of ATGL are not overly convincing. Since ATGListatin is not specific for ATGL (Quiroga et al. 2018), a more thorough and quantitative analysis of TAG turnover with ATGL knockdown/out is warranted if these claims are to be made.

      4) Since DGAT1 mRNA is unchanged but protein goes up, it would be assumed that HILPDA is affecting DGAT1 stability/turnover. This should be considered.

    2. Reviewer #1:

      This study dissects the role of LD associated protein HILPDA in triglyceride and LD homeostasis in hepatic tissue. Using a mouse tissue-specific HILPDA KO, live cell imaging, and lipid analysis, it proposes that HILPDA promotes TAG storage in LDs independently of ATGL regulation. Instead, HILPDA is proposed to interact with DGAT1 and promote TAG synthesis/storage.

      This is an interesting and potentially exciting study that provides a new insight for HILPDA in liver fat storage. The proposed model differs from previous literature that proposes HILPDA regulates lipolysis via ATGL. Unfortunately, while the data presented support a potential role for HILPDA in DGAT regulation, a clear mechanism is not identified. The first half of the paper that phenotypes loss and over-expression of HILPDA is thorough and conclusive. The latter half of the paper, investigating the interplay between HILPDA and DGAT1, appears more preliminary.

      The critical issue in this study is that the nature of the HILPDA-DGAT1 interaction is not well defined. HILPDA over-expression is shown to increase DGAT1 protein levels, but the specific mechanism underlying this is not further dissected. Furthermore, it is still unclear whether this interaction is direct, or merely stochastic due to the fact that both DGAT1 and HILPDA reside on the same LDs in the experiments presented. More biochemical investigation as to whether these proteins physically interact in their native states, and if so whether that interaction affects DGAT1 enzymatic activity directly or allosterically, is required. Without this the study is mainly descriptive.

      Major concerns:

      1) Fig 4: overnight and acute fatty acid addition experiment: The authors propose that HILPDA enriches at sites where new fatty acids are being processed. Can you demonstrate that both these fluorescent FA species are even being incorporated into TAG during the time periods associated with the microscopy? An alternative explanation is simply that HILPDA localizes to regions of the cell where FA esterification or incorporation into other lipid species is occurring. TAG is potentially only one of many fates for these FAs. Can DGAT1/2 be colocalized with HILPDA in these experiments? Alternatively, what happens in these experiments if DGAT inhibitors are co-added with the FAs?

      2) Fig 5H: The DGAT activity assays indicate that HILPDA over-expression increases the incorporation of fluorescent FA and DAG into TAG, but it is unclear as written whether these assays are normalizing for DGAT1 protein amount. Does HILPDA over-expression enhance DGAT enzymatic activity in this panel, or merely promote TAG synthesis here by the increased total DGAT protein level noted later in the study? This is a clear distinction in mechanism, and needs to be dissected further.

      3) Fig 6/7: DGAT1-HILPDA interaction. The data presented in Fig 7 indicate that DGAT1 and HILPDA co-localize in cells and potentially are in very close proximity with one another. However, the data as presented are not enough to indicate whether these proteins directly interact. Do these proteins immunoprecipitate with one another? Some biochemical evidence for their interaction is necessary

      4) Fig 7: relatedly, the mechanism by which DGAT1 is increased in protein level from HILPDA is also unclear. Is the protein more long-lived, or stabilized in the ER when HILPDA is over-expressed? Again, protein biochemical analysis would be helpful.

    3. Preprint Review

      This preprint was reviewed using eLife’s Preprint Review service, which provides public peer reviews of manuscripts posted on bioRxiv for the benefit of the authors, readers, potential readers, and others interested in our assessment of the work. This review applies only to version 2 of the manuscript.

      Summary:

      This study further characterizes the role of lipid droplet (LD) associated protein HILPDA in LD biology. The authors propose that HILPDA promotes triglyceride (TAG) storage in LDs by a mechanism independent of ATGL, through activation of DGAT. This is a potentially interesting finding, however, as detailed by the reviewers below, the data presented do not identify a mechanism for how HILPDA affects DGAT.

    1. We could broadcast a warning if we find the variable to be set in the environment, but that is more likely than not to annoy people who intentionally set it.

      New tag?: warnings that may annoy people who intentionally do something. (Need a way to selectively silence certain warnings?)

    1. Malicious code pushed to your .gitlab-ci.yml file could compromise your variables and send them to a third party server regardless of the masked setting. If the pipeline runs on a protected branch or protected tag, it could also compromise protected variables.
    1. With over 1,500 dating apps on the market, many have come to the conclusion that the romance of courtship has been replaced with fantasy and heavily-edited Instagram photos.  Along with driving this increase in dating apps, the millennial generation is also delaying marriage and moving away from conventional religious practices. Because of this, many popular magazines and TV shows suggest that hook-up culture dominates contemporary pursuits of love. Right-swiping, label free, highly educated, and technologically savvy, today’s young people appear to pursue sex frequently and do so on their own terms. There also appears to be much more equal footing between genders than ever before.

      This is true, young generation does not want to bound themselves in a permanent relationship tag because now they have lots of options due to dating apps.

    1. Although Madisyn applied only one tag of ‘‘Race’’ and did nottag her letter with ‘‘Police,’’ ‘‘Violence,’’ or anything else, her letter speaksto students’ deep and related concerns around discrimination, violence,and specifically the role of police.

      This is observed in two of my letters too. Although the students tag their letters to one topic, they talk about other related issues. For example, in Vivian's letter "Problems in education", she talks about equity in education, standardized testing, and teacher pay. Her letter speaks to deeper and broader issues in education. - Anitha

    1. BIO

      gold data: IOB(inside-outside-beginning) format or BIO

      quote from this link https://towardsdatascience.com/named-entity-recognition-and-classification-with-scikit-learn-f05372f07ba2

      The IOB (short for inside, outside, beginning) is a common tagging format for tagging tokens. I- prefix before a tag indicates that the tag is inside a chunk. B- prefix before a tag indicates that the tag is the beginning of a chunk. An O tag indicates that a token belongs to no chunk (outside).

    Annotators

    1. mouse anti‐DDDDK‐tag

      DOI: 10.1111/acel.13251

      Resource: (MBL International Cat# M185-3, RRID:AB_10950447)

      Curator: @Naa003

      SciCrunch record: RRID:AB_10950447

      Curator comments: anti-DDDDK-tag antibody MBL International Cat# M185-3


      What is this?

  3. akademie-oeffentliches-gesundheitswesen.github.io akademie-oeffentliches-gesundheitswesen.github.io
    1. nach der ein Schwangerschaftsabbruch (SSA) bis zur 12. Woche nach dem 1. Tag der

      Sicher? Ich dachte 14 Wochen ab menstruation und 12 ab Empfängnis?

      https://www.profamilia.de/themen/schwangerschaftsabbruch.html

    1. Doing so also means adding empty import statements to guarantee correct order of evaluation of modules (in ES modules, evaluation order is determined statically by the order of import declarations, whereas in CommonJS – and environments that simulate CommonJS by shipping a module loader, i.e. Browserify and Webpack – evaluation order is determined at runtime by the order in which require statements are encountered).

      Here: dynamic loading (libraries/functions) meaning: at run time

    1. We then made our way to the scanner. After removing all metal objects —including a belt and a stray dry-cleaning tag with a staple

      Everythingmetal need yo be removed?

    Annotators

    1. Someadolescents also create personal sites because their friends have

      Some create their online presence as a communication tool because their friends have it and its the easiest way to reach them, texting can go as far as gifs , voice notes and more but the online precence brings you closer to their online precense as you can tag your friends in funny memes, videos or the coolest trip to plan next , it builds togetherness and interaction without seeing that person face to face but as a means of interaction

    1. In some cases, I could also create a component without any <script> tag at all. So in that way, I could actually bulk up the logic in one place if I could get some help from the #with block.
    1. create catalog manifest and CASE files

      In this stage we create + upload the ibm-appconnect-operator.tar.gz archive.

      1. unstashes 'repo', 'operatormanifestinfo' 'bundlemanifestinfo' 'csv' So that we have access to operator, operator-init, and bundle fat manifest digests. Also access to csv and operator.yaml file.

      2. run the scrpt create-operator-archive.sh which in turn runs the create-catalog-manifest.sh script, which in turn does:

      3. Downland cp4i-operator-bundle-tools.tar.gz from Artifactory and extract it jenkins-build-scripts/cp4i-operator-bundle-tools, that's so to use the push-images-to-er.sh script later on.

      4. call the create-manifest-image-from-platform-images.sh script, which in turn creates the appconnect-operator-catalog fat manifest with the tag ${VERSION}-${BUILD_TIMESTAMP} and pushes it up to appconnect artifactory. it also creates the OperatorCatalogDigest file.

      5. returning back to create-catalog-manifest.sh, update the deploy/catalogsource.yaml file with the new appconnect-operator-catalog fat manifest's digest value

      6. calls the script push-images-to-er.sh in order to copy appconnect-operator-catalog fat manifest to staging Entitled Registry. copied across using tag->digests, then copied across digest->tags. then copied across arch-specific-tags->arch-digests (to enable va scanning).

      7. back in create-catalog-manifest.sh script, in staging ER, assign "latest" to the newly uploaded catalog fat manifest, ${VERSION}-${BUILD_TIMESTAMP}->latest

      8. Returning back to create-operator-archive.sh,

      9. feed the stashed goodimages.json to create-resources-yaml.sh script. This in turn generates the resource.yaml file under the "case" folder.

      10. Curl the cp4i-operator-bundle-tools.tar.gz from artifactory and put it in the jenkins-build-scripts folder. This folder will get ignrore a bit later.

      11. Download cp4i-deploy-operator.tar.gz from artifactory and extract it in the stable/ibm-appconnect-bundle/tests folder

      12. copy the airgap.sh and put it in the stable/ibm-appconnect-bundle/operators/ibm-appconnect/scripts/ folder

      1. copy all "PROD" goodimages.json images into staging ER.

      2. update "latest" tag to now point to the new uploaded gooimages.json images in staging ER.

      3. Also copy some "dev" goodimages.json images into staging ER. That's so that developers can requests images get pull from dockerhub, but actually get pulled from staging ER thanks to openshift registry redirect.

      4. copy the dev images above the /appc bit. so dockerhub urls don't have to specify /appc. Also retag to latest.

      5. Create ibm-appconnect-operator.tar.gz archive. but exclude:

      --exclude "${APP_NAME}/jenkins-build-scripts"

      --exclude "${APP_NAME}/.travis.yml"

      --exclude "${APP_NAME}/.git"

      1. back in jenkinsfile, upload this archive to artifactory in the "latest" folder, this will overwrite what is already there.

      2. Update jenkins job description with info about what has been built.

      3. triggers the job - test-and-promote-cp4i-demo-system. but it doesn't wait for it to succeed.

      resulting files:

      • resources.yaml
    2. create operator manifest image

      runs the create-operator-manifests.sh script:

      1. Downloads cp4i-operator-bundle-tools.tar.gz from artifactory and extracts it content puts it into the jenkins-build-scripts folder, in it's own folder called "cp4i-operator-bundle-tools". Path to this folder is $BUNDLE_TOOLS_DIR. We'll use this archive's push-images-to-er.sh script a bit later on.
      2. Runs the script create-manifest-image-from-platform-images.sh using appconnect-operator as script parameter

      2.1 pulls down each arch image using ${VERSION}-${BUILD_TIMESTAMP}-${arch} tags

      2.2 create docker manifest with tag - ${VERSION}-${BUILD_TIMESTAMP}. And add both entries.

      2.3 push up operator manifest to appconnect artifactory

      2.4 Create the OperatorImageDigest file

      1. do the same for the init image. Which results in the creation of the OperatorInitImageDigest file.
      2. run the script - push-images-to-er.sh to copy both fat manifests to staging ER. It copies using fat manifest digests, followed by tags. It also copies across arch tags for individual images.
      3. Update the operator.yaml file with the fat manifest digests of both appconnect-operator and init image.

      4. create new stash called operatormanifestinfo. This specifically specifies the 2 new top level digest files and the changes made to operator.yaml (with the same digests)

    1. Tagging

      I have found that tagging is really helpful. It might be interesting to see what are common themes.

    2. appreciate your help

      I think that a major part of improving the issue of abuse and providing consent is building in notifications so that website owners will at least be aware that their site is being marked up, highlighted, annotated, and commented on in other locations or by other platforms. Then the site owner at least has the knowledge of what's happening and can then be potentially provided with information and tools to allow/disallow such interactions, particularly if they can block individual bad actors, but still support positive additions, thought, and communication. Ideally this blocking wouldn't occur site wide, which many may be tempted to do now as a knee-jerk reaction to recent events, but would be fine grained enough to filter out the worst offenders.

      Toward the end of notifications to site owners, it would be great if any annotating activity would trigger trackbacks, pingbacks, or the relatively newer and better webmention protocol of the WW3C out of the http://IndieWebCamp.com movement. Then site owners would at least have notifications about what is happening on their site that might otherwise be invisible to them.

      Perhaps there's a way to further implement filters or tools (a la Akismet on platforms like WordPress) that allow site users to mark materials as spam, abusive, or other so that they are then potentially moved from "public" facing to "private" so that the original highlighter can still see their notes, but that the platform isn't allowing the person's own website to act as a platform to give reach to bad actors.

      Further some site owners might appreciate graded filters (G, PG, PG-13, R, X) so that users or even parents can filter what they're willing to see. Consider also annotations on narrative forms that might be posted as spoilers--how can these be guarded against? (Possibly with CSS and a spoiler tag?) Options can be built into the platform itself as well as allowing server-side options for truly hard cases.

      My coding skills are rustier than I wish they were, but I'm available to help/consult if needed.

    1. It is important to note here that the flow does not need to begin with a user interaction. With the rise of asynchronous middleware like redux-saga and redux-observable, the ability to trigger any code on a component anywhere is very useful.

      This tag doesn't quite fit: can be used independently (fine-grained/decoupled)

    1. Resulting articles met inclusion criteria for review if they addressed psychiatric side effects of isotretinoin treatment or the neurobehavioral teratology of isotretinoin.

      Could I do a search like this where I look up mass articles and make sure that they have the same tag words

    1. acknowledges that high priced textbooks are a barrier to learning because many students do not purchase expensive textbooks

      I suspect students also make value judgments--this book is too expensive because that number on the price tag is too high, rather than my financial aid doesn't cover it.

    1. hyperscript is more concise because it's just a function call and doesn't require a closing tag. Using it will greatly simplify your tooling chain.

      I suppose this is also an argument that Python tries to make? That other languages have this con:

      • cons: closing tags make it more verbose / increase duplication and that Python is simpler / more concise because it uses indentation instead of closing delimiters like end or } ?
    1. The primary motivation behind virtual-dom is to allow us to write code independent of previous state. So when our application state changes we will generate a new VTree. The diff function creates a set of DOM patches that, based on the difference between the previous VTree and the current VTree, will update the previous DOM tree to match the new VTree.

      annotation meta: may need new tag: for: "code independent of previous state."

      annotation meta: may need new tag: for: diffs other than source/text code diffs (in this case diffs between virtual DOM trees)

    1. Tag your bestie in the comments!

      I wrote this caption so that it tempts people to tag other people. I posted a funny quote about best friends and asked people to “tag their besties” so they can see the post as well and engage with it further. Here’s an example: https://www.instagram.com/p/CGGacUoHq2p/

    2. @quoteoftheday

      I also tagged a successful account that wrote in their bio, “Tag @quoteoftheday to be featured on our page!” If they feature my post on their account, it could bring a lot of engagement to my own page. Here’s the account: https://www.instagram.com/quoteoftheday/.

    3. Tag your bestie in the comments!

      I wrote this caption so that it tempts people to tag other people. I posted a funny quote about best friends and asked people to “tag their besties” so they can see the post as well and engage with it further. Here’s an example: https://www.instagram.com/p/CGGacUoHq2p/

    4. @quoteoftheday

      I also tagged a successful account that wrote in their bio, “Tag @quoteoftheday to be featured on our page!” If they feature my post on their account, it could bring a lot of engagement to my own page. Here’s the account: https://www.instagram.com/quoteoftheday/.

    1. Curso_HDCICAFCUNAM

      Curso Habilidades digitales y competencias informacionales para ciencias 2020

      FACULTAD DE CIENCIAS, UNAM

      Layla Michán

    1. But first, what would motivate any young person today to pull the plug? Well maybe they should consider this for a moment. Who most wants you to stay on the grid? The advertisers. Your boss. Human Resources. The advertisers. Your parents (irony of ironies – once they distrusted it, now they need to tag you electronically, share your Facebook photos and message you to death). The advertisers. The government. Your local authority. Your school. Advertisers.

      Going of the grid hurts "The man" in 70's parlance.

    1. How do you manage information flows? If anyone is using a personal wiki-style long term information tool I’d love to hear from you!

      I've got a handful of interesting things bookmarked here: https://boffosocko.com/tag/wikis/ which includes a rabbit hole of a request similar to your own.

    1. In short to add wiki-style functionality to my blog, the only functionality that is really needed is that 1) I myself have a edit button on static items, 2) the ability to categorise and tag those items, and 3) keep those items outside of the blog posting stream on the front page, and outside of the RSS feed. WordPress pages fit that description, when I’m logged in, and after adding a plugin to allow categories and tags on pages. So a page based section it is, or rather, will be over time.

      I like the idea of this and the overall structure. It reminds me a bit of Wikity which may provide this functionality plus a bit more. I really need to spin up a version and play around with it to see if it will give me what I'm looking for in terms of a blog linked with wiki-like functionality.

    1. It isn't rocket science, but as Jon indicates, it's incredibly powerful.

      I use my personal website with several levels of taxonomy for tagging and categorizing a variety of things for later search and research.

      Much like the example of the Public Radio International producer, I've created what I call a "faux-cast" because I tag everything I listen to online and save it to my website including the appropriate <audio> link to the.mp3 file so that anyone who wants to follow the feed of my listens can have a playlist of all the podcast and internet-related audio I'm listening to.

      A visual version of my "listened to" tags can be found at https://boffosocko.com/kind/listen/ with the RSS feed at https://boffosocko.com/kind/listen/feed/

    1. When I received Chris’s comment, my first response was that I should delete my post or at least the incorrect part of it. It’s embarrassing to have your incorrect understandings available for public view. But I decided to leave the post as is but put in a disclaimer so that others would not be misled by my misunderstandings. This experience reminded me that learning makes us vulnerable. Admitting that you don’t know something is hard and being corrected is even harder. Chris was incredibly gentle in his correction. It makes me think about how I respond to my students’ work. Am I as gentle with their work as Chris was to mine? Could I be more gentle? How often have I graded my students’ work and only focused on what they did wrong? Or forgotten that feeling of vulnerability when you don’t know something, when you put your work out for others to judge? This experience has also reminded me that it’s important that we as teachers regularly put ourselves into situations in which we authentically grapple with not knowing something. We should regularly share our less than fully formed understandings with others for feedback. It helps us remember that even confident learners can struggle with being vulnerable. And we need to keep in mind that many of our students are not confident learners.

      I'm reminded here of the broad idea that many bloggers write about sooner or later of their website being a "thought space" or place to contemplate out in the open. More often than not, even if they don't have an audience to interact with, their writings become a way of thinking out loud, clarifying things for themselves, self-evolving, or putting themselves out there for potential public reactions (good, bad, or indifferent).

      While writing things out loud to no audience can be helpful and useful on an individual level, it's often even more helpful to have some sort of productive and constructive feedback. While a handful of likes or positive seeming responses can be useful, I always prefer the ones that make me think more broadly, deeply, or force me to consider other pieces I hadn't envisioned before. To me this is the real value of these open and often very public thought spaces.

      For those interested in the general idea, I've been bookmarking/tagging things around the idea of thought spaces I've read on my own website. Hopefully this collection helps others better understand the spectrum of these ideas for themselves.

      With respect to the vulnerability piece, I'm reminded of an episode of <cite>The Human Current</cite> I listened to a few weeks back. There was an excellent section that touched on building up trust with students or even a class when it comes to providing feedback and criticism. Having a bank of trust makes it easier to give feedback as well as to receive it. Here's a link to the audio portion and a copy of the relevant text.

    1. The Task Annotation Project in Science (TAPS) provides K-12 educators with annotated assessment tasks, aligned to the Next Generation Science Standards, that help guide teachers in more equitably monitoring their students’ learning.37 Osmosis is a repository of open educational resources (OER) created to crowdsource the future of medical education.38 Undergraduate and graduate medical students have access to thousands of digital resources, and they have also used annotation - through comments, feedback forms, and ratings - to improve the quality of these learning materials.39 The National Science Digital Library (NSDL), created in 2000, is an archive of open access teaching and learning resources for learners of all ages across science, technology, engineering, and mathematics disciplines.40 Annotation has been used to tag the NSDL’s resources and improve information accessibility, support student interaction with multimedia content through a digital notebook, and educators have annotated NSDL resources to design online learning activities for their students.41 And research about the digital annotation tool Perusall.d-undefined, .lh-undefined { background-color: rgba(0, 0, 0, 0.2) !important; }.d-undefined, .lh-undefined { background-color: rgba(0, 0, 0, 0.5) !important; }3Troy Hicks, Nate Angell, Jeremy Dean, often used in conjunction with science textbooks, has shown that college students’ pre-reading and annotation practices can subsequently improve exam performance.
  4. link-springer-com.uaccess.univie.ac.at link-springer-com.uaccess.univie.ac.at
    1. ites of heightened, future-oriented public debate aboutpossible futures

      tag

    2. ites of heightened, future-oriented public debate aboutpossible futures

      tag

    1. Author Response

      Reviewer #1:

      Hutchings et al. report an updated cryo-electron tomography study of the yeast COP-II coat assembled around model membranes. The improved overall resolution and additional compositional states enabled the authors to identify new domains and interfaces--including what the authors hypothesize is a previously overlooked structural role for the SEC31 C-Terminal Domain (CTD). By perturbing a subset of these new features with mutants, the authors uncover some functional consequences pertaining to the flexibility or stability of COP-II assemblies.

      Overall, the structural and functional work appears reliable, but certain questions and comments should be addressed prior to publication. However, this reviewer failed to appreciate the conceptual advance that warrants publication in a general biology journal like eLIFE. Rather, this study provides a valuable refinement of our understanding of COP-II that I believe is better suited to a more specialized, structure-focused journal.

      We agree that in our original submission our description of the experimental setup, indeed similar to previous work, did not fully capture the novel findings of this paper. Rather than being simply a higher resolution structure of the COPII coat, in fact we have discovered new interactions in the COPII assembly network, and we have probed their functional roles, significantly changing our understanding of the mechanisms of COPII-mediated membrane curvature. In the revised submission we have included additional genetic data that further illuminate this mechanism, and have rewritten the text to better communicate the novel aspects of our work.

      Our combination of structural, functional and genetic analyses goes beyond refining our textbook understanding of the COPII coat as a simple ‘adaptor and cage’, but rather it provides a completely new picture of how dynamic regulation of assembly and disassembly of a complex network leads to membrane remodelling.

      These new insights have important implications for how coat assembly provides structural force to bend a membrane but is still able to adapt to distinct morphologies. These questions are at the forefront of protein secretion, where there is debate about how different types of carriers might be generated that can accommodate cargoes of different size.

      Major Comments: 1) The authors belabor what this reviewer thinks is an unimportant comparison between the yeast reconstruction of the outer coat vertex with prior work on the human outer coat vertex. Considering the modest resolution of both the yeast and human reconstructions, the transformative changes in cryo-EM camera technology since the publication of the human complex, and the differences in sample preparation (inclusion of the membrane, cylindrical versus spherical assemblies, presence of inner coat components), I did not find this comparison informative. The speculations about a changing interface over evolutionary time are unwarranted and would require a detailed comparison of co-evolutionary changes at this interface. The simpler explanation is that this is a flexible vertex, observed at low resolution in both studies, plus the samples are very different.

      We do agree that our proposal that the vertex interface changes over evolutionary time is speculative and we have removed this discussion. We agree that a co-evolutionary analysis will be enlightening here, but is beyond the scope of the current work.

      We respectfully disagree with the reviewer’s interpretation that the difference between the two vertices is due to low resolution. The interfaces are clearly different, and the resolutions of the reconstructions are sufficient to state this. The reviewer’s suggestion that the difference in vertex orientation might be simply attributable to differences in sample, such as inclusion of the membrane, cylindrical versus spherical morphology, or presence of inner coat components were ruled out in our original submission: we resolved yeast vertices on spherical vesicles (in addition to those on tubes) and on membrane-less cages. These analyses clearly showed that neither the presence of a membrane, nor the change in geometry (tubular vs. spherical) affect vertex interactions. These experiments are presented in Supplementary Fig 4 (Supplementary Fig. 3 in the original version). Similarly, we discount that differences might be due to the presence or absence of inner coat components, since membrane-less cages were previously solved in both conditions and are no different in terms of their vertex structure (Stagg et al. Nature 2006 and Cell 2008).

      We believe it is important to report on the differences between the two vertex structures. Nevertheless, we have shifted our emphasis on the functional aspects of vertex formation and moved the comparison between the two vertices to the supplement.

      2) As one of the major take home messages of the paper, the presentation and discussion of the modeling and assignment of the SEC31-CTD could be clarified. First, it isn't clear from the figures or the movies if the connectivity makes sense. Where is the C-terminal end of the alpha-solenoid compared to this new domain? Can the authors plausibly account for the connectivity in terms of primary sequence? Please also include a side-by-side comparison of the SRA1 structure and the CTD homology model, along with some explanation of the quality of the model as measured by Modeller. Finally, even if the new density is the CTD, it isn't clear from the structure how this sub-stoichiometric and apparently flexible interaction enhances stability. Hence, when the authors wrote "when the [CTD] truncated form was the sole copy of Sec31 in yeast, cells were not viable, indicating that the novel interaction we detect is essential for COPII coat function." Maybe, but could this statement be a leap to far? Is it the putative interaction essential, or is the CTD itself essential for reasons that remain to be fully determined?

      The CTD is separated from the C-terminus of the alpha solenoid domain by an extended domain (~350 amino acids) that is predicted to be disordered, and contains the PPP motifs and catalytic fragment that contact the inner coat. This is depicted in cartoon form in Figures 3A and 7, and discussed at length in the text. This arrangement explains why no connectivity is seen, or expected. We could highlight the C-terminus of the alpha-solenoid domain to emphasize where the disordered region should emerge from the rod, but connectivity of the disordered domain to the CTD could arise from multiple positions, including from an adjacent rod.

      The reviewer’s point about the essentiality of the CTD being independent of its interaction with the Sec31 rod, is an important one. The basis for our model that the CTD enhances stability or rigidity of the coat is the yeast phenotype of Sec31-deltaCTD, which resembles that of a sec13 null. Both mutants are lethal, but rescued by deletion of emp24, which leads to more easily deformable membranes (Čopič et al. Science 2012). We agree that even if this model is true, the interaction of the CTD with Sec31 that our new structure reveals is not proven to drive rigidity or essentiality. We have tempered this hypothesis and added alternative possibilities to the discussion.

      We have included the SRA1 structure in Supplementary Fig 5, as requested, and the model z-score in the Methods. The Z-score, as calculated by the proSA-web server is -6.07 (see figure below, black dot), and falls in line with experimentally determined structures including that of the template (PDB 2mgx, z-score = -5.38).

      img

      3) Are extra rods discussed in Fig. 4 are a curiosity of unclear functional significance? This reviewer is concerned that these extra rods could be an in vitro stoichiometry problem, rather than a functional property of COP-II.

      This is an important point, that, as we state in the paper, cannot be answered at the moment: the resolution is too low to identify the residues involved in the interaction. Therefore we are hampered in our ability to assess the physiological importance of this interaction. We still believe the ‘extra’ rods are an important observation, as they clearly show that another mode of outer coat interaction, different from what was reported before, is possible.

      The concern that interactions visualised in vitro might not be physiologically relevant is broadly applicable to structural biology approaches. However, our experimental approach uses samples that result from active membrane remodelling under near-physiological conditions, and we therefore expect these to be less prone to artefacts than most in vitro reconstitution approaches, where proteins are used at high concentrations and in high salt buffer conditions.

      4) The clashsccore for the PDB is quite high--and I am dubious about the reliability of refining sidechain positions with maps at this resolution. In addition to the Ramchandran stats, I would like to see the Ramachandran plot as well as, for any residue-level claims, the density surrounding the modeled side chain (e.g. S742).

      The clashscore is 13.2, which, according to molprobity, is in the 57th percentile for all structures and in the 97th for structures of similar resolutions. We would argue therefore that the clashscore is rather low. In fact, the model was refined from crystal structures previously obtained by other groups, which had worse clashscore (17), despite being at higher resolution. Our refinement has therefore improved the clashscore. During refinement we have chosen restraint levels appropriate to the resolution of our map (Afonine et al., Acta Cryst D 2018)

      The Ramachandran plot is copied here and could be included in a supplemental figure if required. We make only one residue-level claim (S742), the density for which is indeed not visible at our resolution. We claim that S742 is close to the Sec23-23 interface, and do not propose any specific interactions. Nevertheless we have removed reference to S742 from the manuscript. We included this specific information because of the potential importance of this residue as a site of phosphorylation, thereby putting this interface in broader context for the general eLife reader.

      img

      Minor Comments:

      1) The authors wrote "To assess the relative positioning of the two coat layers, we analysed the localisation of inner coat subunits with respect to each outer coat vertex: for each aligned vertex particle, we superimposed the positions of all inner coat particles at close range, obtaining the average distribution of neighbouring inner coat subunits. From this 'neighbour plot' we did not detect any pattern, indicating random relative positions. This is consistent with a flexible linkage between the two layers that allows adaptation of the two lattices to different curvatures (Supplementary Fig 1E)." I do not understand this claim, since the pattern both looks far from random and the interactions depend on molecular interactions that are not random. Please clarify.

      We apologize for the confusion: the pattern of each of the two coats are not random. Our sentence refers to the positions of inner and outer coats relative to each other. The two lattices have different parameters and the two layers are linked by flexible linkers (the 350 amino acids referred to above). We have now clarified the sentence.

      2) Related to major point #1, the author wrote "We manually picked vertices and performed carefully controlled alignments." I do now know what it means to carefully control alignments, and fear this suggests human model bias.

      We used different starting references for the alignments, with the precise aim to avoid model bias. For both vesicle and cage vertex datasets, we have aligned the subtomograms against either the vertex obtained from tubules, or the vertex from previously published membrane-less cages. In all cases, we retrieved a structure that resembles the one on tubules, suggesting that the vertex arrangement we observe isn’t simply the result of reference bias. This procedure is depicted in Supplementary Fig 4 (Supplementary Fig. 3 in the original manuscript), but we have now clarified it also in the methods section.

      3) Why do some experiments use EDTA? I may be confused, but I was surprised to see the budding reaction employed 1mM GMPPNP, and 2.5mM EDTA (but no Magnesium?). Also, for the budding reaction, please replace or expand upon the "the 10% GUV (v/v)" with a mass or molar lipid-to-protein ratio.

      We regret the confusion. As stated in the methods, all our budding reactions are performed in the presence of EDTA and Magnesium, which is present in the buffer (at 1.2 mM). The reason is to facilitate nucleotide exchange, as reported and validated in Bacia et al., Scientific Reports 2011.

      Lipids in GUV preparations are difficult to quantify. We report the stock concentrations used, but in each preparation the amount of dry lipid that forms GUVs might be different, as is the concentration of GUVs after hydration. However since we analyse reactions where COPII proteins have bound and remodelled individual GUVs, we do not believe the protein/lipid ratio influences our structures.

      4) Please cite the AnchorMap procedure.

      We cite the SerialEM software, and are not aware of other citations specifically for the anchor map procedure.

      5) Please edit for typos (focussing, functionl, others)

      Done

      Reviewer #2:

      The manuscript describes new cryo-EM, biochemistry, and genetic data on the structure and function of the COPII coat. Several new discoveries are reported including the discovery of an extra density near the dimerization region of Sec13/31, and "extra rods" of Sec13/31 that also bind near the dimerization region. Additionally, they showed new interactions between the Sec31 C-terminal unstructured region and Sec23 that appear to bridge multiple Sec23 molecules. Finally, they increased the resolution of the Sec23/24 region of their structure compared to their previous studies and were able to resolve a previously unresolved L-loop in Sec23 that makes contact with Sar1. Most of their structural observations were nicely backed up with biochemical and genetic experiments which give confidence in their structural observations. Overall the paper is well-written and the conclusions justified.

      However, this is the third iteration of structure determination of the COPII coat on membrane with essentially the same preparation and methods. Each time, there has been an incremental increase in resolution and new discoveries, but the impact of the present study is deemed to be modest. The science is good, but it may be more appropriate for a more specialized journal. Areas of specific concern are described below.

      As described above, we respectfully disagree with this interpretation of the advance made by the current work. This work improves on previous work in many aspects. The resolution of the outer coat increases from over 40A to 10-12A, allowing visualisation of features that were not previously resolved, including a novel vertex arrangement, the Sec31 CTD, and the outer coat ‘extra rods’. An improved map of the inner coat also allows us to resolve the Sec23 ‘L-loop’. We would argue that these are not just extra details, but correspond to a suite of novel interactions that expand our understanding of the complex COPII assembly network. Moreover, we include biochemical and genetic experiments that not only back up our structural observations but bring new insights into COPII function. As pointed out in response to reviewer 1, we believe our work contributes a significant conceptual advance, and have modified the manuscript to convey this more effectively.

      1) The abstract is vague and should be re-written with a better description of the work.

      We have modified the abstract to specifically outline what we have done and the major new discoveries of this paper.

      2) Line 166 - "Surprisingly, this mutant was capable of tubulating GUVs". This experiment gets to one of the fundamental unknown questions in COPII vesiculation. It is not clear what components are driving the membrane remodeling and at what stages during vesicle formation. Isn't it possible that the tubulation activity the authors observe in vitro is not being driven at all by Sec13/31 but rather Sec23/24-Sar1? Their Sec31ΔCTD data supports this idea because it lacks a clear ordered outer coat despite making tubules. An interesting experiment would be to see if tubules form in the absence of all of Sec13/31 except the disordered domain of Sec31 that the authors suggest crosslinks adjacent Sec23/24s.

      This is an astute observation, and we agree with the reviewer that the source of membrane deformation is not fully understood. We favour the model that budding is driven significantly by the Sec23-24 array. To further support this, we have performed a new experiment, where we expressed Sec31ΔN in yeast cells lacking Emp24, which have more deformable membranes and are tolerant to the otherwise lethal deletion of Sec13. While Sec31ΔN in a wild type background did not support cell viability, this was rescued in a Δemp24 yeast strain, strongly supporting the hypothesis that a major contributor to membrane remodelling is the inner coat, with the outer coat becoming necessary to overcome membrane bending resistance that ensues from the presence of cargo. We now include these results in Figure 1.

      However, we must also take into account the results presented in Fig. 6, where we show that weakening the Sec23-24 interface still leads to budding, but only if Sec13-31 is fully functional, and that in this case budding leads to connected pseudo-spherical vesicles rather than tubes. When Sec13-31 assembly is also impaired, tubes appear unstructured. We believe this strongly supports our conclusions that both inner and outer coat interactions are fundamental for membrane remodelling, and it is the interplay between the two that determines membrane morphology (i.e. tubes vs. spheres).

      To dissect the roles of inner and outer coats even further, we have done the experiment that the reviewer suggests: we expressed Sec31768-1114, but the protein was not well-behaved and co-purified with chaperones. We believe the disordered domain aggregates when not scaffolded by the structured elements of the rod. Nonetheless, we used this fragment in a budding reaction, and could not see any budding. We did not include this experiment as it was inconclusive: the lack of functionality of the purified Sec31 fragment could be attributed to the inability of the disordered region to bind its inner coat partner in the absence of the scaffolding Sec13-31 rod. As an alternative approach, we have used a version of Sec31 that lacks the CTD, and harbours a His tag at the N-terminus (known from previous studies to partially disrupt vertex assembly). We think this construct is more likely to be near native, since both modifications on their own lead to functional protein. We could detect no tubulation with this construct by negative stain, while both control constructs (Sec31ΔCTD and Nhis-Sec31) gave tubulation. This suggests that the cross-linking function of Sec31 is not sufficient to tubulate GUV membranes, but some degree of functional outer coat organisation (either mediated by N- or C-terminal interactions) is needed. It is also possible that the lack of outer coat organisation might lead to less efficient recruitment to the inner coat and cross-linking activity. We have added this new observation to the manuscript.

      3) Line 191 - "Inspecting cryo-tomograms of these tubules revealed no lozenge pattern for the outer 192 coat" - this phrasing is vague. The reviewer thinks that what they mean is that there is a lack of order for the Sec13/31 layer. Please clarify.

      The reviewer is correct, we have changed the sentence.

      4) Line 198 - "unambiguously confirming this density corresponds to 199 the CTD." This only confirms that it is the CTD if that were the only change and the Sec13/31 lattice still formed. Another possibility is that it is density from other Sec13/31 that only appears when the lattice is formed such as the "extra rods". One possibility is that the density is from the extra rods. The reviewer agrees that their interpretation is indeed the most likely, but it is not unambiguous. The authors should consider cross-linking mass spectrometry.

      We have removed the word ‘unambiguously’, and changed to ‘confirming that this density most likely corresponds to the CTD’. Nonetheless, we believe that our interpretation is correct: the extra rods bind to a different position, and themselves also show the CTD appendage. In this experiment, the lack of the CTD was the only biochemical change.

      5) In the Sec31ΔCTD section, the authors should comment on why ΔCTD is so deleterious to oligomer organization in yeast when cages form so abundantly in preparations of human Sec13/31 ΔC (Paraan et al 2018).

      We have added a comment to address this. “Interestingly, human Sec31 proteins lacking the CTD assemble in cages, indicating that either the vertex is more stable for human proteins and sufficient for assembly, or that the CTD is important in the context of membrane budding but not for cage formation in high salt conditions.”

      6) The data is good for the existence of the "extra rods", but significance and importance of them is not clear. How can these extra densities be distinguished from packing artifacts due to imperfections in the helical symmetry.

      Please also see our response to point 3 from reviewer 1. Regarding the specific concern that artefacts might be a consequence of imperfection in the helical symmetry, we would argue such imperfections are indeed expected in physiological conditions, and to a much higher extent. For this reason interactions seen in the context of helical imperfections are likely to be relevant. In fact, in normal GTP hydrolysis conditions, we expect long tubes would not be able to form, and the outer coat to be present on a wide range of continuously changing membrane curvatures. We think that the ability of the coat to form many interactions when the symmetry is imperfect might be exactly what confers the coat its flexibility and adaptability.

      7) Figure 5 is very hard to interpret and should be redone. Panels B and C are particularly hard to interpret.

      We have made a new figure where we think clarity is improved.

      8) The features present in Sec23/24 structure do not reflect the reported resolution of 4.7 Å. It seems that the resolution is overestimated.

      We report an average resolution of 4.6 Å. In most of our map we can clearly distinguish beta strands, follow the twist of alpha helices and see bulky side chains. These features typically become visible at 4.5-5A resolution. We agree that some areas are worse than 4.6 Å, as typically expected for such a flexible assembly, but we believe that the average resolution value reported is accurate. We obtained the same resolution estimate using different software including relion, phenix and dynamo, so that is really the best value we can provide. To further convince ourselves that we have the resolution we claim, we sampled EM maps from the EMDB with the same stated resolution (we just took the 7 most recent ones which had an associated atomic model), and visualised their features at arbitrary positions. For both beta strands and alpha helices, we do not feel our map looks any worse than the others we have examined. We include a figure here.

      img

      9) Lines 315/316 - "We have combined cryo-tomography with biochemical and genetic assays to obtain a complete picture of the assembled COPII coat at unprecedented resolution (Fig. 7)"

      10) Figure 7. is a schematic model/picture the authors should reference a different figure or rephrase the sentence.

      We now refer to Fig 7 in a more appropriate place.

      Reviewer #3:

      The manuscript by Hutchings et al. describes several previously uncharacterised molecular interactions in the coats of COP-II vesicles by using a reconstituted coats of yeast COPI-II. They have improved the resolution of the inner coat to 4.7A by tomography and subtomogram averaging, revealing detailed interactions, including those made by the so-called L-loop not observed before. Analysis of the outer layer also led to new interesting discoveries. The sec 31 CTD was assigned in the map by comparing the WT and deletion mutant STA-generated density maps. It seems to stabilise the COP-II coats and further evidence from yeast deletion mutants and microsome budding reconstitution experiments suggests that this stabilisation is required in vitro. Furthermore, COP-II rods that cover the membrane tubules in right-handed manner revealed sometimes an extra rod, which is not part of the canonical lattice, bound to them. The binding mode of these extra rods (which I refer to here a Y-shape) is different from the canonical two-fold symmetric vertex (X-shape). When the same binding mode is utilized on both sides of the extra rod (Y-Y) the rod seems to simply insert in the canonical lattice. However, when the Y-binding mode is utilized on one side of the rod and the X-binding mode on the other side, this leads to bridging different lattices together. This potentially contributes to increased flexibility in the outer coat, which maybe be required to adopt different membrane curvatures and shapes with different cargos. These observations build a picture where stabilising elements in both COP-II layers contribute to functional cargo transport. The paper makes significant novel findings that are described well. Technically the paper is excellent and the figures nicely support the text. I have only minor suggestions that I think would improve the text and figure.

      We thank the reviewer for helpful suggestions which we agree improve the manuscript.

      Minor Comments:

      L 108: "We collected .... tomograms". While the meaning is clear to a specialist, this may sound somewhat odd to a generic reader. Perhaps you could say "We acquired cryo-EM data of COP-II induced tubules as tilt series that were subsequently used to reconstruct 3D tomograms of the tubules."

      We have changed this as suggested

      L 114: "we developed an unbiased, localisation-based approach". What is the part that was developed here? It seems that the inner layer particle coordinates where simply shifted to get starting points in the outer layer. Developing an approach sounds more substantial than this. Also, it's unclear what is unbiased about this approach. The whole point is that it's biased to certain regions (which is a good thing as it incorporates prior knowledge on the location of the structures).

      We have modified the sentence to “To target the sparser outer coat lattice for STA, we used the refined coordinates of the inner coat to locate the outer coat tetrameric vertices”, and explain the approach in detail in the methods.

      L 124: "The outer coat vertex was refined to a resolution of approximately ~12 A, revealing unprecedented detail of the molecular interactions between Sec31 molecules (Supplementary Fig 2A)". The map alone does not reveal molecular interactions; the main understanding comes from fitting of X-ray structures to the low-resolution map. Also "unprecedented detail" itself is somewhat problematic as the map of Noble et al (2013) of the Sec31 vertex is also at nominal resolution of 12 A. Furthermore, Supplementary Fig 2A does not reveal this "unprecedented detail", it shows the resolution estimation by FSC. To clarify, these points you could say: "Fitting of the Sec31 atomic model to our reconstruction vertex at 12-A resolution (Supplementary Fig 2A) revealed the molecular interactions between different copies of Sec31 in the membrane-assembled coat.

      We have changed the sentence as suggested.

      L 150: Can the authors exclude the possibility that the difference is due to differences in data processing? E.g. how the maps amplitudes have been adjusted?

      Yes, we can exclude this scenario by measuring distances between vertices in the right and left handed direction. These measurements are only compatible with our vertex arrangement, and cannot be explained by the big deviation from 4-fold symmetry seen in the membrane-less cage vertices.

      L 172: "that wrap tubules either in a left- or right-handed manner". Don't they do always both on each tubule? Now this sentence could be interpreted to mean that some tubules have a left-handed coat and some a right-handed coat.

      We have changed this sentence to clarify. “Outer coat vertices are connected by Sec13-31 rods that wrap tubules both in a left- and right-handed manner.”

      L276: "The difference map" hasn't been introduced earlier but is referred to here as if it has been.

      We now introduce the difference map.

      L299: Can "Secondary structure predictions" denote a protein region "highly prone to protein binding"?

      Yes, this is done through DISOPRED3, a feature include in the PSIPRED server we used for our predictions. The reference is: Jones D.T., Cozzetto D. DISOPRED3: precise disordered region predictions with annotated protein-binding activity Bioinformatics. 2015; 31:857–863. We have now added this reference to the manuscript.

      L316: It's true that the detail in the map of the inner coat is unprecedented and the model presented in Figure 7 is partially based on that. But here "unprecedented resolution" sounds strange as this sentence refers to a schematic model and not a map.

      We have changed this by moving the reference to Fig 7 to a more appropriate place

      L325: "have 'compacted' during evolution" -> remove. It's enough to say it's more compact in humans and less compact in yeast as there could have been different adaptations in different organisms at this interface.

      We have changed as requested. See also our response to reviewer 1, point 1.

      L327: What's exactly meant by "sequence diversity or variability at this density".

      We have now clarified: “Since multiple charge clusters in yeast Sec31 may contribute to this interaction interface (Stancheva et al., 2020), the low resolution could be explained by the fact that the density is an average of different sequences.”

      L606-607: The description of this custom data processing approach is difficult to follow. Why is in-plane flip needed and how is it used here?

      Initially particles are picked ignoring tube directionality (as this cannot be assessed easily from the tomograms due to the pseudo-twofold symmetry of the Sec23/24/Sar1 trimer). So the in plane rotation of inner coat subunit could be near 0 or 180°. For each tube, both angles are sampled (in-plane flip). Most tubes result in the majority of particles being assigned one of the two orientations (which is then assumed as the tube directionality). Particles that do not conform are removed, and rare tubes where directionality cannot be determined are also removed. We have re-written the description to clarify these points: “Initial alignments were conducted on a tube-by-tube basis using the Dynamo in-plane flip setting to search in-plane rotation angles 180° apart. This allowed to assign directionality to each tube, and particles that were not conforming to it were discarded by using the Dynamo dtgrep_direction command in custom MATLAB scripts”

      L627: "Z" here refers to the coordinate system of aligned particles not that of the original tomogram. Perhaps just say "shifted 8 pixels further away from the membrane".

      Changed as requested.

      L642-643: How can the "left-handed" and "right-handed" rods be separated here? These terms refer to the long-range organisation of the rods in the lattice it's not clear how they were separated in the early alignments.

      They are separated by picking only one subset using the dynamo sub-boxing feature. This extracts boxes from the tomogram which are in set positions and orientation relative to the average of previously aligned subtomograms. From the average vertex structure, we sub-box rods at 4 different positions that correspond to the centre of the rods, and the 2-fold symmetric pairs are combined into the same dataset. We have clarified this in the text: “The refined positions of vertices were used to extract two distinct datasets of left and right-handed rods respectively using the dynamo sub-boxing feature.”

      Figure 2B. It's difficult to see the difference between dark and light pink colours.

      We have changed colours to enhance the difference.

      Figure 3C. These panels report the relative frequency of neighbouring vertices at each position; "intensity" does not seem to be the right measure for this. You could say that the colour bar indicates the "relative frequency of neighbouring vertices at each position" and add detail how the values were scaled between 0 and 1. The same applies to SFigure 1E.

      Changed as requested.

      Figure 4. The COP-II rods themselves are relatively straight, and they are not left-handed or right-handed. Here, more accurate would be "architecture of COPII rods organised in a left-handed manner". (In the text the authors may of course define and then use this shorter expression if they so wish.) Panel 4B top panel could have the title "left-handed" and the lower panel should have the title "right-handed" (for consistency and clarity).

      We have now defined left- and right-handed rods in the text, and have changed the figure and panel titles as requested.

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      Reply to the reviewers

      Response to Reviewers and Revision Plan

      We thank all three reviewers for their time and their comments on our manuscript.

      Reviewer #1 (Evidence, reproducibility and clarity (Required)):

      Here Ryan et al. have used localization analysis following induced rapid relocalization of endogenous proteins to investigate the composition and recruitment hierarchy of a clathrin-TACC3-based spindle complex that is important for microtubule organization and stability.

      The authors generate different HeLa cell lines, each with one of four complex members (TACC3, CLTA, chTOG and GTSE1) endogenously tagged with FKBP-GFP via Cas9-mediated editing. This tag allows rapid recruitment to the mitochondria upon rapamycin addition ("knocksideways"). They ultimately quantify each of the 4 components' localization to the spindle following knocksideways of each component using fluorescently-tagged transfected constructs. The authors' interpretation of the results of this analysis are summarized in the last model figure, in which a core MT-binding complex of clathrin and TACC3 recruit the ancillary components GTSE1 and chTOG. In addition, the authors investigate the contribution of individual clathrin-binding LIDL motifs in GTSE1 to the recruitment of clathrin and GTSE1 to spindles. Their findings here largely agree with and confirm a recent report regarding the contribution of these motifs to GTSE1 recruitment to the spindle. They further analyzed GTSE1 fragments for interphase and mitotic microtubule localization, and identified a second region of GTSE1 required (but not sufficient) for spindle localization. Finally, the authors report that PIK3C2A is not part of this complex, contradicting (correcting) a previously published study.

      **Major comments:**

      1.The chTOG-FKBP-GFP cell line the authors generate has only a small fraction of chTOG tagged, and thus should not be used for any conclusions about protein localization dependency on chTOG. Because they were unable to construct a HeLa cell line with all copies tagged, the authors expect that the homozygous knock-in of chTOG-FKBP-GFP is lethal, and thus their experience is appropriate to report. However, the authors should not use this cell line alone to make statements about chTOG dependency. They would have to use similar localization analysis, but after another method to disrupt chTOG (as a second-best approach), such as RNAi. In fact, they have reported this in a previous publication (Booth et al 2011). However, the result was different. There, loss of chTOG resulted in reduced clathrin on spindles, suggesting it may stabilize or help recruit the complex. Alternatively, they could remove their chTOG data, but this would compromise the "comprehensive" nature of the work.

      The referee is correct. The point here is to show the results we had using this approach for all four proteins under study. For this reason, we do not want to remove this data and prefer to show our results “warts-and-all”. We feel that the shortcomings of our approach are honestly presented and discussed in the manuscript. While only a fraction of chTOG was tagged, we should expect some co-removal after its induced mislocalization. Since we saw no change, we concluded that chTOG is auxiliary.

      The “second best” approach suggested (RNAi of chTOG) is problematic for two reasons. First, chTOG RNAi results in gross changes to spindle structure (multipolar spindles) and it is difficult to pick apart differences in protein partner localization that result from loss of chTOG from those resulting from changes in spindle structure. Second, the paper is about induced mislocalization as a method for determining protein complexes once a normal spindle has formed. So, removing chTOG prior to mitosis is not comparable. If we get the same or different result, does it confirm or conflict with the data we have? Nonetheless, given the discrepancy with our earlier work, we should investigate this further.

      To address this concern, we will stain endogenous clathrin, TACC3 and GTSE1 following chTOG RNAi and measure their relative levels at the spindle.

      Making the chTOG-FKBP-GFP cell line was difficult. As described in the paper, we only recovered heterozygous clones despite repeated attempts. Since submission, we have been made aware of a HCT116 chTOG-FKBP-GFP cell line that is reported to be homozygously tagged (Cherry et al. 2019 doi: 10.1002/glia.23628).

      A note about this cell line has been added to the paper (Results section, final sentence of 1st paragraph).

      2.The authors initially analyze complex member localization after knocksideways experiments by antibody staining, which has the advantage of analyzing endogenous proteins (versus the later transfected fluorescent constructs). Setting aside potential artefacts from fixation, this would seem to be a better method for controlled analysis to take advantage of their setup (short of generating stable cell lines with second proteins endogenously tagged in a second color - a huge undertaking). The authors conclude that antibody specificity problems confounded their analysis and explained unusual results. However, I think is worth investing a little more effort to sort this out, rather than bringing doubt to the whole data set. Verifying and then using another antibody for chTOG localization would be informative. Of course, the negative control should not be their chTOG-FKBP-GFP line, as it does not relocalize most of chTOG.

      In the case of GTSE1, an alternative explanation to antibody specificity issues would be that the GTSE1-FKBP-GFP cell line is not in fact homozygously tagged. Given the low expression levels on the western provided, and the detection of GTSE1 on the spindle in the induced GTSE1-FKBP-GFP cell line (but not TACC3-FKBP-GFP), it seems plausible that an untagged copy remains. If there are multiple copies of GTSE1 in Hela cells, one untagged copy could represent a small fraction of total GTSE1. This should thus be ruled out. GTSE1 clones should be analyzed with more protein extracts loaded - dilutions of the extracts can determine the sensitivity of the blot to lower protein levels. In addition, sequencing of genomic DNA can reveal a small percentage with different reads.

      We used a two-pronged approach for assessing relocalization of protein partners (staining vs transfected constructs). The staining approach is superior since endogenous proteins are examined, but it is limited by antibody specificity. The transfection approach overcomes this limitation but is in turn limited by effects of overexpression and tagging. Together the two approaches allow us, and anyone employing this method, to get a picture of protein complexes. We didn’t want to create the impression that one or other approach is confounded, but the referee is correct that this analysis would benefit from further work.

      Specifically, to address these concerns:

      • We will verify and use alternative chTOG antibodies to try to improve this dataset.
      • We will test the possibility that an untagged allele of GTSE1 remains. We will use western blotting and a summary of our genomic analysis will be added to the paper.

        3.There is a lot of data contained in the small graphs summarizing quantification of localization in Figs 3 and 4. They would be more accessible to the reader if they were larger and/or an "example" of the chart with labels was present explaining it (essentially what is in the figure legends). Furthermore, there is no statistical test applied to this data that I see. This is needed. How do authors determine whether there is an "effect"?

      Our aim was to compress a lot of information into a small space, while still showing some example primary data. All reviewers raised the same concern which tells us that we went too far towards “data visualization”.

      To address this point, we will rework these figures.

      **Minor issues:**

      1.The GTSE1 constructs used for mutation and localization analysis are 720 amino acids long. A recent study analyzing similar mutations uses a 739 amino acid construct (Rondelet et al 2020). The latter is the predominant transcript in NCBI and Ensembl databases. It appears the construct used by the authors omits the first 19 a.a.. I do not think using the truncated transcript affects conclusions of the manuscript, but it could generate confusion when identifying residues based on a.a.#s of mutant constructs (Fig 6). This should be somehow clarified.

      We were aware of the longer transcript but were using the 720 residue form since it is the canonical sequence in Uniprot (https://www.uniprot.org/uniprot/Q9NYZ3). We did not know that the 739 form is the predominant transcript. We agree this is unlikely to affect our work but that the numbering may cause confusion.

      We have added a note to the Methods (Molecular Biology section) to accurately describe what we and Rondelet et al. have used.

      2.The labeling of constructs in Fig 6C/D is confusing, and appears shifted by eye at places. Please relabel this more clearly.

      Apologies for the error.

      We have relabeled Figure 6C,D and also made a similar alteration to Figure 5C.

      The recommended new experimental data (Analysis complex member levels on spindles after full perturbation of spindle chTOG; new chTOG antibody stainings in the FKBP lines; reanalysis of GTSE1 DNA/protein in GTSE1-FKBP line) should only require a new antibody/siRNA, plus a few weeks time to repeat the analyses already in the paper with new reagents.

      Reviewer #1 (Significance (Required)):

      While multiple individual components of this complex have been previously characterized, the structure and nature of the complex formation and its recruitment to microtubules/spindles remains a complex problem that has yet to be solved.

      Overall this study represents a comprehensive localization-dependency analysis of the Clathrin-TACC3 based spindle complex using a consistent methodology. Although several of the conclusions of the findings echo previous reports, some of the previous literature is contradictory within itself as well as with the conclusions here. Analyzing all components with a single, rapid-perturbation technique thus has great value to present a clear data set, given that the experimental setup conditions and analysis are solid (a goal to which the majority of comments refer).

      Beyond the complex localization/recruitment analysis, two novel findings of this study that emerge are:

      a)GTSE1 contains a second, separate protein region, distinct from the clathrin-binding motifs that is required for its localization to the spindle, and most likely a microtubule-interaction site. This suggests that GTSE1 recruitment to the spindle is more complex than previously reported.

      b)PI3KC2A, which has been reported previously to be a stabilizing member of this complex, is in fact not a member, nor localizes to spindles, nor displays a mitotic defect after loss. This is important conclusion to be made as it would correct the literature, and avoid future confusion.

      --

      Reviewer #2 (Evidence, reproducibility and clarity (Required)):

      In this paper, the authors investigate the nature of interactions between members of the TACC3-chTOG-clathrin-GTSE1 complex on the mitotic spindle. By using a series of HeLa cell lines that they have created by CRISPR/Cas9 editing to enable spatial manipulation (knocksideways) of either TACC3, chTOG, clathrin and GTSE1, they show that on spindle microtubules TACC3 and clathrin represent core complex members whereas chTOG and GTSE1 bind to them respectively but not to each other. Additionally, the authors find that the protein PIK3C2A, which has been implicated in this complex previously is in fact not a component of this complex in mitotic cells. The main advance of the paper in my opinion is the endogenous tagging of the proteins for knocksideways experiments since former experiments depended on RNAi silencing and expression of tagged proteins from plasmids, which introduced issues of protein silencing efficiency and plasmid overexpression problems. This approach seems to alleviate these problems, except in the case of chTOG which seems to be lethal in its homozygous variant.

      **Major comments:**

      I find the key conclusions regarding the localization of the components of the complex convincing. There are some issues regarding the specificity of antibodies in immunostaining experiments (Fig 3.) and the influence of mCherry-TACC3 expression on distorted localization of the complex prior to knocksideways. However, I think the general conclusion about which complex components (clathrin and TACC3) influence the localization of the other proteins in the complex (chTOG and GTSE1) stands. One thing that I miss from the paper is the data on the consequences on the spindle shape and morphology after knocksideways. I have noticed on images in both Figure 3 and Figure 4 that in some cases distribution of the signal seems to influence quite a bit the spindle morphology. Also, In Figure 3 I have noticed what seems to me a quite big variation in spindle size in tubulin signal in both untreated and rapamycin cells. Since authors have many of these images already, I believe it would be realistic, not costly and of additional value for the paper to provide more data on the consequences of the knocksideways experiments. Change of spindle size, tubulin intensity and DNA/kinetochore misalignment upon knocksideways would be helpful to appreciate more the findings of the paper. More so since the authors on more than one occasion find their motivation in the field of cancer research and spindle stability relation to it. Some data connection to this motivation would be of value. Experiments seem reproducible.

      The focus of the paper is on using the knocksideways methodology to understand a protein complex during mitosis, rather than looking at its function. We are not keen to do new experiments that are not part of the central message of the paper. However, the Reviewer is correct that we do already have a dataset that can be mined in the manner described.

      To address this point, we will analyze spindle size parameters and also the intensity of tubulin. Our analysis will be limited to the short timeframe of our experiments, but it should reveal or refute any changes in spindle structure that may result from loss of complex members.

      **Minor comments:**

      I have some problems with the clarity of Figure 3 and 4. For Figure 3. In Figure 3 plots on the right are a bit small and not easy to read. Some reorganization of the figure might be beneficial. In Figure 4 plots to the right are also too small to be clear. Also, I miss the number of cells (n) I can't see the number of individual arrows because of the size of graphs.

      Our aim was to compress a lot of information into a small space, while still showing some example primary data. All reviewers raised the same concern which tells us that we went too far towards “data visualization”.

      To address this point, we will rework these figures.

      Reviewer #2 (Significance (Required)):

      I find that the biggest significance of the paper is in the creation of new tools (cell lines) to study the localization of proteins TACC3, chTOG, clathrin and GTSE1. Cell lines where endogenous proteins can be delocalized rapidly will be of value for scientist working not only in mitosis but such as in the case of clathrin research, vesicle formation and trafficking or p53-dependent apoptosis in the case of GTSE1. In the field of mitosis it will surely help and speed up the research concerning the role of these proteins in spindle assembly and stability.

      Field of expertise: mitotic spindle

      --

      Reviewer #3 (Evidence, reproducibility and clarity (Required)):

      **Summary:**

      This papers analyses the chTog/TACC3/clathrin/GTSE1 complex that crosslinks and stabilises microtubule bundles in the mitotic spindle. The authors have developed an elegant knock sideways approach to specifically analyse the effects of removing individual components of the complex from the spindle and study the effect this has on the other interactors. They report, based on these assays that the core of the complex is formed by TACC3 and Clathrin while GTSE1 and chTog are auxiliary interactors. They also refute previous evidence that this complex also incorporates PIK3C2A. Overall, this is an interesting study that distinguishes itself predominantly by its methodology. However, some of the reported results need more thorough analysis to allow convincing conclusions.

      **Major comments:**

      1)The knockside way method is the main highlight if this paper. Unlike previous studies by the PI, this time endogenous genes are tagged which is a key advance and allows much better interpretation of the results. I am not sure why the authors have chosen HeLa cells as their model here, given the messed up genome of these cells. A non-transformed cell line would have been preferable, but as a proof of principle study, I think HeLa are acceptable, and I wouldn't expect the authors to repeat all the experiment in another system.

      Figure 1,2 and S1 are describing and validating this approach in some detail, but this will require some more work.

      The authors state that gene targeting was validated using a combination of PCR, sequencing, Western blotting, but show only the results for westerns. PCR analysis that demonstrates homozygous or heterozygous gene targeting should be shown here.

      Another issue is the penetrance of the phenotypes induced by Rapamycin. The authors show nice data of the system working in individual cells but do not give us an idea if this happens in all cells. The localisation of the individual tagged genes should be quantified (ideally with line plots) in 50 randomly chosen mitotic cells with 3 repeats before and after rapamycin treatment. Moreover, the analysis of mitotic duration (Figure S1D) should be extended to include a plus Rapamycin cohort and this should be moved in the main Figure.

      If the system works only in a small proportion of cells, this should be clearly stated. I don't think this would prevent publication, but it is an important piece of information that is missing.

      The Reviewer raises two issues here.

      • PCR analysis should be shown. This issue was also partly raised by Reviewer 1. A summary of our PCR analysis was actually included in Table 1, since the analysis we did is pretty unwieldy. We agree though that presenting our evidence for homozygosity of the cell lines would be useful. To address this point, we will add more detail of the PCR and sequencing work done to validate these cell lines.
      • Does knocksideways happen in all cells? The answer to this depends on the transient expression of MitoTrap and sufficient application of rapamycin. We agree that this will be a useful piece of information to add to the manuscript. A related issue is whether knocksideways of complex members affects mitotic progression. We have established through other experiments that rapamycin application to wild-type cells alters mitotic progression, although application of Rapalog does not have this effect. Our plan to address these points is 1) to analyze the efficacy of knocksideways that readers can expect to achieve using these, or similar cells, and 2) analyze mitotic duration in rapalog-treated cells expressing a rapalog sensitive MitoTrap.

        2)Apart from a simple quantification of mitotic duration, I believe a more detailed mitotic phenotype analysis for each knock-side way gene, especially the homozygous targeted clones, should be included. This can involve more high-resolution live cell imaging of mitotic progression with SiR-DNA and GFP-tubulin, using the dark mitotrap.

      We don’t agree that such an analysis should be included. The focus of this paper is on using the knocksideways methodology to understand a protein complex during mitosis, and not looking at its function. There are several papers on the mitotic phenotypes of these genes probed using RNAi in different cellular systems (examples for chTOG: 10.1101/gad.245603; TACC3/clathrin: 10.1038/emboj.2011.15, 10.1242/jcs.075911, 10.1083/jcb.200911091, 10.1083/jcb.200911120; GTSE1: 10.1083/jcb.201606081). Moreover, our 2013 paper used knocksideways (with RNAi and overexpression) and has a detailed analysis of mitotic progression, microtubule stability, checkpoint activity and kinetochore motions (Cheeseman et al., 2013 doi: 10.1242/jcs.124834).

      New experiments that are not part of the central message of the paper and are unlikely to give new insight are not the best use of our revision efforts for this paper (especially during the pandemic). Having said this, Reviewer 2’s suggestion to use our existing dataset to investigate mitotic phenotypes, will largely answer Reviewer 3’s request.

      We will analyze spindle size parameters and also the intensity of tubulin. Our analysis will be limited to the short timeframe of our experiments, but it should reveal or refute any changes in spindle structure that result from the loss of complex members.

      3)Overall, the quantitative analysis in Figure 3 ,4 and 7 is not good enough and sometimes doesn't fully support the conclusions. In Figure 3,4 a convoluted way of demonstrating the change in localisation is shown and this panel is so small that is almost impossible to read. Also, there is no statistical analysis, and the sample size seems very small . At least 25 cells should be analysed here in 3 repeats. I would suggest to unify the quantification in the MS and use the line plots shown in Figure 5 and 6 and compare each protein before and after rapamycin addition. This is much easier to read and more convincing. The images of the cells panels can be moved to a supplement as they contain very little information. This would generate space to expand the size and depth of the quantitative analysis. Instead of Anova tests, I would recommend using a simple t-test comparing each condition to its relevant control since this is the only relevant comparison in the experiment. Statistical significance should be calculated for each experiment with sufficient sample size. It would also be better to show the individual data points from the three repeats in different colours so that the reproducibility between repeat can be judged.

      This type of statistical analysis should be uniformly done throughout the MS and also extended to Figure 7.

      The referee raises several issues here with our data presentation and statistical analysis.

      • Our aim in Figures 3 and 4 was to compress a lot of information into a small space, while still showing some example primary data. All reviewers raised the same concern about these figures which tells us that we went too far towards “data visualization”. To address this point, we will rework Figures 3 and 4 to provide more clear data presentation.
      • The Reviewer’s comments about statistical analysis however are not sound. First, it is incorrect to state that simple t-tests can be applied (this is a form of p-hacking). Correction for multiple testing must be done on these datasets. Second, the reviewer arbitrarily states numbers for cells and experimental repeats without considering the effect size or it seems, understanding the structure of the data that we have collected. Sample sizes are small but they are taken from many independent replicates. Third, and related to the previous point, the fixed and live cell data are structured differently which means that a uniform data presentation is not possible. The live data has a paired design and each cell is an independent replicate (with replicates done over several trials). The fixed data is unpaired and we have taken measures from several experiments (independent replicates). The point about applying statistical tests to the data is also made by Reviewer 1 and we will use appropriate tests (NHST or estimation statistics) as we re-work the figures.

        Reviewer #3 (Significance (Required)):

      In my opinion, the most interesting aspect of the MS is the methodology. Based on this, publication is justified and will be of interest to a wider audience. That is why a more detailed analysis of the penetrance of this manipulation across the cell population will be critical.

      The application of this method to analyse the composition of the TACC3/Clathrin complex on the spindle is the main biological advance, and the novel information is rather limited but not unimportant.

      Overall, if these results can be properly quantified I would recommend publication.

    2. Note: This preprint has been reviewed by subject experts for Review Commons. Content has not been altered except for formatting.

      Learn more at Review Commons


      Referee #1

      Evidence, reproducibility and clarity

      Here Ryan et al. have used localization analysis following induced rapid relocalization of endogenous proteins to investigate the composition and recruitment hierarchy of a clathrin-TACC3-based spindle complex that is important for microtubule organization and stability. The authors generate different HeLa cell lines, each with one of four complex members (TACC3, CLTA, chTOG and GTSE1) endogenously tagged with FKBP-GFP via Cas9-mediated editing. This tag allows rapid recruitment to the mitochondria upon rapamycin addition ("knocksideways"). They ultimately quantify each of the 4 components' localization to the spindle following knocksideways of each component using fluorescently-tagged transfected constructs. The authors' interpretation of the results of this analysis are summarized in the last model figure, in which a core MT-binding complex of clathrin and TACC3 recruit the ancillary components GTSE1 and chTOG. In addition, the authors investigate the contribution of individual clathrin-binding LIDL motifs in GTSE1 to the recruitment of clathrin and GTSE1 to spindles. Their findings here largely agree with and confirm a recent report regarding the contribution of these motifs to GTSE1 recruitment to the spindle. They further analyzed GTSE1 fragments for interphase and mitotic microtubule localization, and identified a second region of GTSE1 required (but not sufficient) for spindle localization. Finally, the authors report that PIK3C2A is not part of this complex, contradicting (correcting) a previously published study.

      Major comments:

      1.The chTOG-FKBP-GFP cell line the authors generate has only a small fraction of chTOG tagged, and thus should not be used for any conclusions about protein localization dependency on chTOG. Because they were unable to construct a HeLa cell line with all copies tagged, the authors expect that the homozygous knock-in of chTOG-FKBP-GFP is lethal, and thus their experience is appropriate to report. However, the authors should not use this cell line alone to make statements about chTOG dependency. They would have to use similar localization analysis, but after another method to disrupt chTOG (as a second-best approach), such as RNAi. In fact, they have reported this in a previous publication (Booth et al 2011). However, the result was different. There, loss of chTOG resulted in reduced clathrin on spindles, suggesting it may stabilize or help recruit the complex. Alternatively, they could remove their chTOG data, but this would compromise the "comprehensive" nature of the work.

      2.The authors initially analyze complex member localization after knocksideways experiments by antibody staining, which has the advantage of analyzing endogenous proteins (versus the later transfected fluorescent constructs). Setting aside potential artefacts from fixation, this would seem to be a better method for controlled analysis to take advantage of their setup (short of generating stable cell lines with second proteins endogenously tagged in a second color - a huge undertaking). The authors conclude that antibody specificity problems confounded their analysis and explained unusual results. However, I think is worth investing a little more effort to sort this out, rather than bringing doubt to the whole data set. Verifying and then using another antibody for chTOG localization would be informative. Of course, the negative control should not be their chTOG-FKBP-GFP line, as it does not relocalize most of chTOG.

      In the case of GTSE1, an alternative explanation to antibody specificity issues would be that the GTSE1-FKBP-GFP cell line is not in fact homozygously tagged. Given the low expression levels on the western provided, and the detection of GTSE1 on the spindle in the induced GTSE1-FKBP-GFP cell line (but not TACC3-FKBP-GFP), it seems plausible that an untagged copy remains. If there are multiple copies of GTSE1 in Hela cells, one untagged copy could represent a small fraction of total GTSE1. This should thus be ruled out. GTSE1 clones should be analyzed with more protein extracts loaded - dilutions of the extracts can determine the sensitivity of the blot to lower protein levels. In addition, sequencing of genomic DNA can reveal a small percentage with different reads.

      3.There is a lot of data contained in the small graphs summarizing quantification of localization in Figs 3 and 4. They would be more accessible to the reader if they were larger and/or an "example" of the chart with labels was present explaining it (essentially what is in the figure legends). Furthermore, there is no statistical test applied to this data that I see. This is needed. How do authors determine whether there is an "effect"?

      Minor issues:

      1.The GTSE1 constructs used for mutation and localization analysis are 720 amino acids long. A recent study analyzing similar mutations uses a 739 amino acid construct (Rondelet et al 2020). The latter is the predominant transcript in NCBI and Ensembl databases. It appears the construct used by the authors omits the first 19 a.a.. I do not think using the truncated transcript affects conclusions of the manuscript, but it could generate confusion when identifying residues based on a.a.#s of mutant constructs (Fig 6). This should be somehow clarified.

      2.The labeling of constructs in Fig 6C/D is confusing, and appears shifted by eye at places. Please relabel this more clearly.

      The recommended new experimental data (Analysis complex member levels on spindles after full perturbation of spindle chTOG; new chTOG antibody stainings in the FKBP lines; reanalysis of GTSE1 DNA/protein in GTSE1-FKBP line) should only require a new antibody/siRNA, plus a few weeks time to repeat the analyses already in the paper with new reagents.

      Significance

      While multiple individual components of this complex have been previously characterized, the structure and nature of the complex formation and its recruitment to microtubules/spindles remains a complex problem that has yet to be solved.

      Overall this study represents a comprehensive localization-dependency analysis of the Clathrin-TACC3 based spindle complex using a consistent methodology. Although several of the conclusions of the findings echo previous reports, some of the previous literature is contradictory within itself as well as with the conclusions here. Analyzing all components with a single, rapid-perturbation technique thus has great value to present a clear data set, given that the experimental setup conditions and analysis are solid (a goal to which the majority of comments refer).

      Beyond the complex localization/recruitment analysis, two novel findings of this study that emerge are:

      a)GTSE1 contains a second, separate protein region, distinct from the clathrin-binding motifs that is required for its localization to the spindle, and most likely a microtubule-interaction site. This suggests that GTSE1 recruitment to the spindle is more complex than previously reported.

      b)PI3KC2A, which has been reported previously to be a stabilizing member of this complex, is in fact not a member, nor localizes to spindles, nor displays a mitotic defect after loss. This is important conclusion to be made as it would correct the literature, and avoid future confusion.

    1. The general justification for appropriating the tag has been that, in addition to killing Black people, White supremacy also continues to kill and harm a lot of non-Black people of color as well.

      This year we have really highlighted the tragic deaths of many black people. We call people out for their racist behaviors and views. We are getting closer to uncovering the corruption some people have and cancelling them for having them. In this case I feel cancel culture would be legitimate because of how these people are looking down upon groups of people.

    1. Congress appears to have missed a key point in its questioning last week. It’s clear that fake news and outright lies are, in fact, a small portion of the total content on any of the big tech platforms. But what matters are the routes that these companies provide to unreliable sources of information. You don’t have to silence Julian Assange or some random Twitter account that’s set up to look like a real news outfit, but you also don’t have to inject them into a legitimate news discussion.

      For something to pop up on the top "news" section, I think these developers should have to fact check and read through the information. They could also add a tag such as potentially untruthful which would help stop spread the misinformation..

    1. Why Are Finland’s Schools Successful? The country’s achievements in education have other nations, especially the United States, doing their homework <img src="https://thumbs-prod.si-cdn.com/thzZYTv2Evhq3x8iHdcaakihfVE=/800x600/filters:no_upscale()/https://public-media.si-cdn.com/filer/cd/ee/cdee1c82-f8e3-4de4-983e-8599d4485745/finland-smiles-wr.jpg" alt="Kirkkojarvi School" itemprop="image"> "This is what we do every day," says Kirkkojarvi Comprehensive School principal Kari Louhivuori, "prepare kids for life." (Stuart Conway) By LynNell Hancock Smithsonian Magazine | Subscribe September 2011 AddThis Sharing ButtonsShare to FacebookFacebookShare to TwitterTwitterShare to RedditReddit78Share to PinterestPinterest997Share to LinkedInLinkedInShare to FlipboardFlipboardShare to EmailEmailShare to PrintPrintShare to MoreAddThis934 It was the end of term at Kirkkojarvi Comprehensive School in Espoo, a sprawling suburb west of Helsinki, when Kari Louhivuori, a veteran teacher and the school’s principal, decided to try something extreme—by Finnish standards. One of his sixth-grade students, a Kosovo-Albanian boy, had drifted far off the learning grid, resisting his teacher’s best efforts. The school’s team of special educators—including a social worker, a nurse and a psychologist—convinced Louhivuori that laziness was not to blame. So he decided to hold the boy back a year, a measure so rare in Finland it’s practically obsolete. function dispatchComscoreLoadedEvent(){ let event = new Event('MPlayerComscoreLoaded'); window.dispatchEvent(event); } !function(e){var t={};function n(r){if(t[r])return t[r].exports;var i=t[r]={i:r,l:!1,exports:{}};return e[r].call(i.exports,i,i.exports,n),i.l=!0,i.exports}n.m=e,n.c=t,n.d=function(e,t,r){n.o(e,t)||Object.defineProperty(e,t,{enumerable:!0,get:r})},n.r=function(e){"undefined"!==typeof Symbol&&Symbol.toStringTag&&Object.defineProperty(e,Symbol.toStringTag,{value:"Module"}),Object.defineProperty(e,"__esModule",{value:!0})},n.t=function(e,t){if(1&t&&(e=n(e)),8&t)return e;if(4&t&&"object"===typeof e&&e&&e.__esModule)return e;var r=Object.create(null);if(n.r(r),Object.defineProperty(r,"default",{enumerable:!0,value:e}),2&t&&"string"!=typeof e)for(var i in e)n.d(r,i,function(t){return e[t]}.bind(null,i));return r},n.n=function(e){var t=e&&e.__esModule?function(){return 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t=a.store,n=t.getState,r=t.dispatch,i=gn.volume(n());Bn()||gn.muted(n())?(e.setVolume(0),Qi(!0)(r)):(e.setVolume(gn.volume(n())),eo(i)(r),Qi(!1)(r))}),f()(this,"createIMAAdManager",function(t){a.IMAAdManager=t.getAdsManager(a.adVideoElement,e.getAdsRenderingSettings()),a.setAdVolume(a.IMAAdManager)}),f()(this,"registerToAdManagerEvents",function(){a.IMAAdManager.addEventListener(google.ima.AdErrorEvent.Type.AD_ERROR,a.onAdError),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.CONTENT_PAUSE_REQUESTED,a.onContentPauseRequested),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.CONTENT_RESUME_REQUESTED,a.onContentResumeRequested),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.STARTED,a.onAdStarted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.IMPRESSION,a.onAdImpression),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.SKIPPED,a.onAdSkipped),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.COMPLETE,a.onAdCompleted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.PAUSED,a.onAdPaused),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.RESUMED,a.onAdStarted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.AD_PROGRESS,a.onAdProgressChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.VOLUME_CHANGED,a.onVolumeChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.VOLUME_MUTED,a.onAdVolumeMutedChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.ALL_ADS_COMPLETED,a.onAdCompleted)}),f()(this,"onIMAAdsManagerLoaded",function(e){var t=a.store.dispatch;a.createIMAAdManager(e),a.registerToAdManagerEvents(),Zi("loaded")(t)}),f()(this,"onAdError",function(e){var t=a.store.dispatch;!function(e){return function(t){t({type:"[MONETIZATION] change ad error",payload:e})}}(e.getError().getMessage())(t),Ji(!1),a.continuePlayingContent()}),f()(this,"onAdImpression",function(e){var t=a.store.dispatch,n=!e.getAd().g.vpaid;a.setPodInfo(e),function(e){e({type:"[MONETIZATION] increase ad impression counter"})}(t),function(e){return function(t){t({type:"[MONETIZATION] update is vast ad",payload:e})}}(n)(t)}),f()(this,"onVolumeChanged",function(e){var t=a.store.dispatch;eo(e.target.getVolume())(t)}),f()(this,"onAdVolumeMutedChanged",function(e){var t=a.store.dispatch;0===e.target.getVolume()?Qi(!0)(t):Qi(!1)(t)}),f()(this,"continuePlayingContent",function(){var e=a.store,t=e.getState,n=e.dispatch,r=hn.videoTagStatus(t());Xi("idle"===r?"play":"resume")(n)}),f()(this,"stopPlayingContent",function(){var e=a.store.dispatch;Xi("pause")(e)}),f()(this,"onContentPauseRequested",function(){a.stopPlayingContent()}),f()(this,"onContentResumeRequested",function(){a.continuePlayingContent()}),f()(this,"onAdPaused",function(){var e=a.store.dispatch;Zi("paused")(e)}),f()(this,"setPodInfo",function(e){var t=e&&e.getAd()&&e.getAd().getAdPodInfo();if(!Un(t)){var n=a.store.dispatch;!function(e,t){return function(n){n({type:"[MONETIZATION] change pod info",payload:{slotNumber:e,podNumber:t}})}}(t.getAdPosition(),a.totalAdRequestMadeAmount)(n)}}),f()(this,"onAdStarted",function(){var e=a.store,t=e.dispatch,n=e.getState,r=gn.volume(n());Zi("playing")(t),0===a.IMAAdManager.getVolume()?a.IMAAdManager.setVolume(0):window.shouldPlayAdRule||a.IMAAdManager.setVolume(r),a.onResize()}),f()(this,"onAdCompleted",function(){var e=a.store.dispatch;Zi("completed")(e)}),f()(this,"onAdSkipped",function(){var e=a.store.dispatch;Zi("skipped")(e)}),f()(this,"onResize",function(){Un(a.IMAAdManager)||(a.IMAAdManager.resize(a.videoPlayerElement.clientWidth,a.videoPlayerElement.clientHeight,google.ima.ViewMode.NORMAL),a.adContainerElement.style.height="".concat(a.videoPlayerElement.clientHeight,"px"))}),f()(this,"onAdProgressChanged",function(e){var t,n,r=a.store,i=r.dispatch,o=r.getState,s=e.getAdData().currentTime,u=e.getAdData().duration,c=_i.adDuration(o());(t=s,function(e){e({type:"[MONETIZATION] change ad current time",payload:t})})(i),c!==u&&(n=u,function(e){e({type:"[MONETIZATION] change ad duration",payload:n})})(i)}),f()(this,"onAnchorStatusChanged",function(){var e=a.store.getState;"processing"!==Pr(e())&&a.onResize()}),f()(this,"changeAdVolume",function(e){Un(a.IMAAdManager)||a.IMAAdManager.setVolume(e)}),f()(this,"changeAdMuted",function(e,t){Un(a.IMAAdManager)||(t?a.IMAAdManager.setVolume(0):a.IMAAdManager.setVolume(e))}),f()(this,"changeAdStatus",function(e){Un(a.IMAAdManager)||("playing"===e&&a.IMAAdManager.resume(),"paused"===e&&a.IMAAdManager.pause())});var s=t.getState;this.store=t,this.adVideoElement=r,this.videoPlayerElement=i,this.adContainerElement=n,this.adDisplayContainer=new google.ima.AdDisplayContainer(n,r),this.createAdLoader(s(),this.adDisplayContainer),this.adDisplayContainer.initialize(),this.anchorStatusStoreSubscriber=new ji(t,e.getAnchorDependencies,this.onAnchorStatusChanged.bind(this)),this.registerForWindowResize(),this.initMutationObserver(o)};f()(to,"getAdsRenderingSettings",function(){var e=new google.ima.AdsRenderingSettings;return e.restoreCustomPlaybackStateOnAdBreakComplete=!0,e.enablePreloading=!1,e.uiElements=[],e.loadVideoTimeout=15e3,e}),f()(to,"getAnchorDependencies",function(e){return[Pr(e)]});var no=function e(t,n,r,i,o,a){var s=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"playerId",void 0),f()(this,"adScheduler",void 0),f()(this,"adHandler",void 0),f()(this,"imaLoadingStatusSubscriber",void 0),f()(this,"adStatusSubscriber",void 0),f()(this,"videoTagStatusSubscriber",void 0),f()(this,"adContainer",void 0),f()(this,"adVideoElement",void 0),f()(this,"videoPlayerElement",void 0),f()(this,"playerContainer",void 0),f()(this,"pendingMidrollAdPlay",!1),f()(this,"pendingPrerollAdPlay",!1),f()(this,"pendingPrerollAdTag",null),f()(this,"pendingMidrollNumber",null),f()(this,"pendingAdStatusStoreSubscriber",void 0),f()(this,"adMutedStoreSubscriber",void 0),f()(this,"adVolumeStoreSubscriber",void 0),f()(this,"onMidrollAdOpportunity",function(){var e=s.store,t=e.dispatch,n=e.getState,r=_i.adStatus(n()),i=bi.continuePlayingWhileWaitingForAd(n());"loaded"===r?s.playAd(!0):"requested"===r&&(s.pendingMidrollAdPlay=!0,i||(Xi("pause")(t),Ji(!0)(t))),function(e){e({type:"[MONETIZATION] increase ad Opportunity counter"})}(t)}),f()(this,"onPrerollAdOpportunity",function(e){var t=s.store,n=t.getState,r=t.dispatch,i=Fi.loadingImaStatus(n());Un(s.adHandler)?"loading"!==i&&""!==i||(Ji(!0)(r),s.pendingPrerollAdPlay=!0,s.pendingPrerollAdTag=e):(s.pendingPrerollAdPlay=!0,Ji(!0)(r),s.adHandler.loadNewAd(e,"preroll"))}),f()(this,"onPreMidrollAdOpportunity",function(e,t){Un(s.adHandler)||(e.currentTime>=e.midrollTime&&(s.pendingMidrollAdPlay=!0),s.pendingMidrollNumber=e.midrollNumber,s.adHandler.loadNewAd(t,"midroll"))}),f()(this,"hasPendingAd",function(){return s.hasPendingMidrollAdPlay()||s.hasPendingPrerollAdPlay()}),f()(this,"onAdStatusChanged",function(e){var t=s.store.dispatch,n=_i.adStatus(e);"completed"===n&&Ji(!1)(t);var r=bi.continuePlayingWhileWaitingForAd(e),i=_i.loadingAd(e);"playing"!==n&&"error"!==n||r||!i||Ji(!1)(t),s.hasPendingAd()&&"loaded"===n?s.playAd(s.hasPendingMidrollAdPlay()):s.hasPendingAd()&&"error"===n?(Ji(!1),s.clearPendingMidroll(),s.clearPendingPreroll()):Hi(n)||(Ji(!1),function(e){e({type:"[MONETIZATION] clear ad data"})}(t))}),f()(this,"clearPendingMidroll",function(){s.pendingMidrollNumber=null,s.pendingMidrollAdPlay=!1}),f()(this,"clearPendingPreroll",function(){s.pendingPrerollAdPlay=!1,s.pendingPrerollAdTag=null}),f()(this,"onVideoTagStatusChanged",function(e){"complete"===hn.videoTagStatus(e)&&function(e){e({type:"[MONETIZATION] clear played midrolls"})}(s.store.dispatch)}),f()(this,"hasPendingMidrollAdPlay",function(){return s.pendingMidrollAdPlay}),f()(this,"hasPendingPrerollAdPlay",function(){return s.pendingPrerollAdPlay}),f()(this,"playAd",function(e){var t,n=s.store.dispatch,r=s.adHandler.playAd();e?((t=s.pendingMidrollNumber,function(e){e({type:"[MONETIZATION] add 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Ki(t,this),this.adStatusSubscriber=new ji(t,e.getAdStatusDependencies,this.onAdStatusChanged.bind(this)),this.videoTagStatusSubscriber=new ji(t,e.getVideoTagStatusDependencies,this.onVideoTagStatusChanged.bind(this)),e.canUseIMA(u())?this.adHandler=new to(t,r,i,o,a):this.imaLoadingStatusSubscriber=new ji(t,e.getIMALoadingStatusDependencies,this.onIMALoadingStatusChanged.bind(this)),this.pendingAdStatusStoreSubscriber=new ji(t,e.getPendingAdStatusDependencies,this.onPendingAdStatusChanged.bind(this)),this.adMutedStoreSubscriber=new ji(t,e.getAdMutedDependencies,this.onAdMutedChanged.bind(this)),this.adVolumeStoreSubscriber=new ji(t,e.getAdVolumeDependencies,this.onAdVolumeChanged.bind(this))};f()(no,"getAdStatusDependencies",function(e){return[_i.adStatus(e)]}),f()(no,"getVideoTagStatusDependencies",function(e){return[hn.videoTagStatus(e)]}),f()(no,"getIMALoadingStatusDependencies",function(e){return[Fi.loadingImaStatus(e)]}),f()(no,"canUseIMA",function(e){return"success"===Fi.loadingImaStatus(e)}),f()(no,"getPendingAdStatusDependencies",function(e){return[_i.pendingAdStatus(e)]}),f()(no,"getAdMutedDependencies",function(e){return[_i.adMuted(e)]}),f()(no,"getAdVolumeDependencies",function(e){return[_i.adVolume(e)]});var ro=function(e,t){!function(e,t){var n=document.getElementById(vn(t));B(b(Li,{store:e,playerId:t}),n)}(e,t);var n=function(e){var t=Ri(e);return document.getElementById(t)}(t),r=function(e){var t=Bn()?Di(e):En(e);return document.getElementById(t)}(t),i=function(e){var t=En(e);return document.getElementById(t)}(t),o=function(e){var t=bn(e);return document.getElementById(t)}(t);return new no(e,t,n,r,i,o)},io=n(4),oo=n.n(io),ao=n(7),so=n.n(ao),uo=function(){function e(){Ai()(this,e),f()(this,"duration",void 0),f()(this,"position",void 0),f()(this,"previousPosition",void 0),f()(this,"loadTime",void 0),f()(this,"adOrder",void 0),f()(this,"adType",void 0),f()(this,"adDuration",void 0),f()(this,"errorMessage",void 0),f()(this,"adPodNumber",void 0),f()(this,"adSlotNumber",void 0)}return Vi()(e,[{key:"setDuration",value:function(e){return this.duration=e,this}},{key:"setPosition",value:function(e){return this.position=e,this}},{key:"setPreviousPosition",value:function(e){return this.previousPosition=e,this}},{key:"setLoadTime",value:function(e){return this.loadTime=e,this}},{key:"setAdOrder",value:function(e){return this.adOrder=e,this}},{key:"setAdType",value:function(e){return this.adType=e,this}},{key:"setAdDuration",value:function(e){return this.adDuration=e,this}},{key:"setErrorMessage",value:function(e){return this.errorMessage=e,this}},{key:"setAdPodNumber",value:function(e){return this.adPodNumber=e,this}},{key:"setAdSlotNumber",value:function(e){return this.adSlotNumber=e,this}},{key:"build",value:function(){var e=[];return jn(this.position)||e.push("video current position=".concat(Hn(this.position),"sec")),jn(this.duration)||e.push("video duration time=".concat(Hn(this.duration),"sec")),jn(this.loadTime)||e.push("video load time=".concat(this.loadTime,"milliseconds")),jn(this.previousPosition)||e.push("previous position=".concat(Hn(this.previousPosition),"sec")),jn(this.adOrder)||e.push("ad order=".concat(this.adOrder)),jn(this.adType)||e.push("ad type=".concat(this.adType)),jn(this.adDuration)||e.push("ad duration=".concat(Hn(Number(this.adDuration)),"sec")),jn(this.adPodNumber)||e.push("pod number=".concat(this.adPodNumber)),jn(this.adSlotNumber)||e.push("slot number=".concat(this.adSlotNumber)),jn(this.errorMessage)||e.push("error message=".concat(this.errorMessage)),e.join(";")}}]),e}(),co="mmPlus GTM data ready to GA",lo="mmPlus GTM event to GA",po={EMBED:"vplayer video player embed",FIRST_PLAY:"vplayer video first play",COMPLETION_25_PERCENTAGE:"vplayer video 25% complete",COMPLETION_50_PERCENTAGE:"vplayer video 50% complete",COMPLETION_75_PERCENTAGE:"vplayer video 75% complete",COMPLETION_90_PERCENTAGE:"vplayer video 90% complete",AD_BLOCK:"vplayer video ad block",AD_REQUEST:"vplayer video ad request",AD_IMPRESSION:"vplayer video ad impression",AD_ERROR:"vplayer video ad error",AD_VIEWABLE_IMPRESSION:"vplayer video ad viewable impression",AD_COMPLETE:"vplayer video ad complete",AD_SKIP:"vplayer video ad skip",AD_PAUSE:"vplayer video ad pause",VIDEO_COMPLETE:"vplayer video complete",FULLSCREEN_ON:"vplayer video fullscreen on",FULLSCREEN_OFF:"vplayer video fullscreen off",SEEK:"vplayer video position seeked",VIDEO_MUTE:"vplayer video mute",VIDEO_UNMUTE:"vplayer video unmute",CONTROLS_MUTE_OR_UNMUTE:"controls 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t=gn.fullscreen(e),n=hn.currentVideoTimeFragment(e),i=(new uo).setPosition(n).build(),o=t?po.FULLSCREEN_ON:po.FULLSCREEN_OFF;r.analyticsEventsCallbacks.onEvent(o,i)}),this.store=t,this.analyticsEventsCallbacks=n,this.videoMuteSubscriber=new ji(t,e.getVideoMuteDependencies,this.onMuteStateChanged.bind(this)),this.videoFullscreenSubscriber=new ji(t,e.getVideoFullscreenDependencies,this.onFullsScreenStateChanged.bind(this))};f()(yo,"getVideoMuteDependencies",function(e){return[gn.muted(e)]}),f()(yo,"getVideoFullscreenDependencies",function(e){return[gn.fullscreen(e)]});var go=n(3),vo=n.n(go),mo=n(8),bo=n.n(mo),Oo=n(9),_o=n.n(Oo),So=n(5),Eo=n.n(So);n(20);var wo={root:null,threshold:.5,rootMargin:"0px"},Po=function(){function e(t,n,r){Ai()(this,e),f()(this,"store",void 0),f()(this,"observableElement",void 0),f()(this,"callback",void 0),f()(this,"isViewableTimeoutHandler",null),f()(this,"observer",void 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e=this.store.getState;this.didReport=!0,this.callback(e()),this.unobserve()}}]),e}();function To(e){var t=function(){if("undefined"===typeof Reflect||!Reflect.construct)return!1;if(Reflect.construct.sham)return!1;if("function"===typeof Proxy)return!0;try{return Date.prototype.toString.call(Reflect.construct(Date,[],function(){})),!0}catch(e){return!1}}();return function(){var n,r=Eo()(e);if(t){var i=Eo()(this).constructor;n=Reflect.construct(r,arguments,i)}else n=r.apply(this,arguments);return _o()(this,n)}}var Ao=function(e){bo()(n,e);var t=To(n);function n(e,r,i){var o;return Ai()(this,n),o=t.call(this,e,r,i),f()(vo()(o),"videoTagStatusSubscriber",void 0),f()(vo()(o),"onVideoTagStatusChanged",function(e){var t=hn.videoTagStatus(e);"playing"===t?o.onPlay():"paused"===t||"seeking"===t?o.onPause():"complete"!==t&&"error"!==t||o.onComplete()}),o.videoTagStatusSubscriber=new ji(e,n.getVideoTagStatusDependencies,o.onVideoTagStatusChanged.bind(vo()(o))),o}return 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t=hn.videoTagStatus(e),n=Cn.activeVideoIndex(e);if(!(n===o.firstPlayReportedIndex)&&"playing"===t){o.firstPlayReportedIndex=n;var r=o.getFirstPlayLabel(e);o.analyticsEventsCallbacks.onEvent(po.FIRST_PLAY,r)}}),f()(this,"reportVideoViewableImpression",function(e){var t=hn.currentVideoTimeFragment(e),n=(new uo).setPosition(t).build();o.analyticsEventsCallbacks.onEvent(po.CONTENT_VIEWABLE_IMPRESSION,n)}),this.store=t,this.analyticsEventsCallbacks=n,this.videoViewableImpressionObserver=new Ao(t,r,this.reportVideoViewableImpression.bind(this)),this.videoTagStatusSubscriber=new ji(t,e.getVideoTagDependencies,this.onVideoTagStatusChanged.bind(this)),this.registerVideoCallbacksIdNeeded(t.getState(),i)};f()(Co,"getVideoTagDependencies",function(e){return[hn.videoTagStatus(e)]});var Ro=[25,50,75,90],Do=function(){function e(){Ai()(this,e),f()(this,"lastReportedPercentage",void 0),this.lastReportedPercentage=0}return 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fa(fa({},e),{},{currentVideoBufferedTime:t.payload});case"[CORE] update video current duration":return fa(fa({},e),{},{currentVideoDuration:t.payload});case"[CORE] change video tag status":return fa(fa({},e),{},{videoTagStatus:t.payload});case"[CORE] update player visibility":return fa(fa({},e),{},{playerVisibility:t.payload});case"[CORE] update placeholder visibility":return fa(fa({},e),{},{playerPlaceholderVisibility:t.payload});case"[CORE] change loading player status":return fa(fa({},e),{},{loadingPlayer:t.payload});case"[COMMON] show black screen with loader":return fa(fa({},e),{},{loader:fa(fa({},e.loader),{},{showBlackScreen:t.payload})});case"[CORE] set player size":return fa(fa({},e),{},{playerSize:t.payload});case"[COMMON] set error message":return fa(fa({},e),{},{errorMessage:t.payload});default:return e}},brandingData:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:va,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return ga({},function(e,t){var n=t.powered_by_strip,r=t.brand_logo,i=t.brand_logo_click_url,o=t.brand_color;return ga(ga({},e),{},{showVoltaxLogo:Un(n)?e.showVoltaxLogo:n,brandingLogoSrc:Un(r)?e.brandingLogoSrc:r,brandingLogoUrl:Un(i)?e.brandingLogoUrl:i,brandingColor:Un(o)?e.brandingColor:o})}(e,t.payload.initiateParams));default:return e}},anchorOptions:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Oa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return ba({},function(e,t){var n=t.anchor_options;if(!Un(n)){var r=n.anchoring_appearance,i=n.can_close,o=n.closable_ad,a=n.close_after,s=n.continue_streaming,u=n.orientation,c=n.margins,l=n.sticky_below_class_name,d=n.width,p=Un(c)?e.margins:{top:Number.isInteger(c.top)?c.top:e.margins.top,bottom:Number.isInteger(c.bottom)?c.bottom:e.margins.bottom,left:Number.isInteger(c.left)?c.left:e.margins.left,right:Number.isInteger(c.right)?c.right:e.margins.right};return ba(ba({},e),{},{anchoringAppearance:r||e.anchoringAppearance,canClose:Un(i)?e.canClose:i,orientation:Un(u)?e.orientation:u,closableAd:Un(o)?e.closableAd:o,closeAfter:Un(a)?e.closeAfter:a,continueStreaming:Un(s)?e.continueStreaming:s,stickyBelowClassName:Un(l)?e.stickyBelowClassName:l,width:Un(d)?e.width:d,margins:p,anchorData:ba(ba({},e.anchorData),{},{anchorEnabled:!0})})}return e}(e,t.payload.initiateParams));case"[COMMON] set anchor enable":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorEnabled:t.payload})});case"[ANCHOR] update is anchor status":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorStatus:t.payload})});case"[COMMON] set anchor disabled by user":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorDisabledByUser:t.payload})});default:return e}},monetization:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:wa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Sa({},function(e,t){var n=t.monetization;if(Un(n))return e;var r=n.ad_tag,i=n.ad_type,o=n.vpaid_mode,a=n.ad_request_timeout,s=n.continue_content_play_while_waiting_for_ad,u=n.midrolls,c=u&&u.on&&u.on.sort(Wn),l=Un(s)?e.continuePlayingWhileWaitingForAd:s,d=c?c.indexOf(0):-1,p=-1!==d&&!l;return p&&(c=c.splice(d,1)),Sa(Sa({},e),{},{midrolls:Sa(Sa({},e.midrolls),{},{every:u&&u.every,on:c}),prerollEnabled:p,adRequestTimeout:Un(a)?e.adRequestTimeout:parseInt(a,10),vpaidMode:Un(o)?e.vpaidMode:o,continuePlayingWhileWaitingForAd:l,adsData:Sa(Sa({},e.adsData),{},{adType:Un(i)?e.adsData.adType:i,adTagUrlTemplate:Un(r)?e.adsData.adTagUrlTemplate:r})})}(e,t.payload.initiateParams));case"[COMMON] set new ad tag url template":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adTagUrlTemplate:t.payload})});case"[MONETIZATION] change ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adStatus:t.payload,adErrorMessage:null})});case"[MONETIZATION] change ad tag":var n=t.payload,r=n.adUnit,i=n.adTag;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{currentAdTag:i,adUnit:r})});case"[MONETIZATION] change pending ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{pendingAdStatus:t.payload})});case"[MONETIZATION] change ad error":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adStatus:"error",adErrorMessage:t.payload})});case"[MONETIZATION] increase ad impression counter":var o=e.adsData.adImpression;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adImpression:o+1})});case"[MONETIZATION] increase ad Opportunity counter":var a=e.adsData.adOpportunity;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOpportunity:a+1})});case"[MONETIZATION] add played midroll number":var s=e.adsData.playedMidrolls,u=In()(s);return u.push(t.payload),Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOrder:t.payload,playedMidrolls:u})});case"[MONETIZATION] clear played midrolls":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{playedMidrolls:[]})});case"[MONETIZATION] clear ad data":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOrder:0,currentAdTag:null,adDuration:0,adUnit:""})});case"[MONETIZATION] change ad duration":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adDuration:t.payload})});case"[MONETIZATION] update is vast ad":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{isVastAd:t.payload})});case"[MONETIZATION] change ad current time":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adCurrentTime:t.payload})});case"[MONETIZATION] update ad muted":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adMuted:t.payload})});case"[MONETIZATION] change ad volume":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adVolume:t.payload})});case"[MONETIZATION] change pod info":var c=t.payload,l=c.podNumber,d=c.slotNumber;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{podNumber:l,slotNumber:d})});case"[MONETIZATION] change loading ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{loadingAd:t.payload})});default:return e}},mediaData:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:ja,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Va({},function(e,t){var n=t.content_type,r=t.media_id,i=t.display_title;return Va(Va({},e),{},{mediaType:Un(n)?e.mediaType:n,mediaId:Un(r)?e.mediaId:r,videoData:Va(Va({},e.videoData),{},{showTitle:!!Un(i)||i})})}(e,t.payload.initiateParams));case"[CORE] load video request":return Va(Va({},e),{},{loadingMedia:!0});case"[CORE] load video request success":return Va(Va({},e),{},{loadingMedia:!1,videoList:t.payload});case"[CORE] set current video":var n=t.payload,r=n.index,i=n.videoData;return Va(Va({},e),{},{activeVideoIndex:r,videoData:i});case"[CORE] load video request error":return Va(Va({},e),{},{loadingMedia:!1,mediaLoadingError:t.payload});case"[COMMON] media request":var o=t.payload.mediaRequestObject;return Va(Va({},e),{},{mediaRequest:Va({},o)});default:return e}},semanticOptions:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Ba,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Fa({},function(e,t){var n=t.semantic_options;if(Un(n))return e;var r=n.minimum_date_factor,i=n.promoted_videos,o=n.scan_images_on_page,a=n.scanned_element,s=n.scanned_element_type,u=n.scoped_keywords,c=n.tags;return Fa(Fa({},e),{},{minimumDateFactor:Un(r)?e.minimumDateFactor:r,promotedVideos:Un(i)?e.promotedVideos:i,scanImagesOnPage:Un(o)?e.scanImagesOnPage:o,scannedElement:Un(a)?e.scannedElement:a,scannedElementType:Un(s)?e.scannedElementType:s,scopedKeywords:Un(u)?e.scopedKeywords:u,tags:Un(c)?e.tags:c})}(e,t.payload.initiateParams));default:return e}},userInteraction:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Wa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[USER INTERACTION] change user interaction":return qa(qa({},e),{},{userInteractionType:t.payload});default:return e}},splitView:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:$a,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Ga({},function(e,t){var n=t.anchor_options;if(!Un(n)){var r=n.split_view,i=n.split_view_ratio;return Ga(Ga({},e),{},{splitViewRatio:Un(r)||!r||Un(i)?e.splitViewRatio:i})}return e}(e,t.payload.initiateParams));default:return e}},discovery:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Za,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Ya({},function(e,t){var n=t.next_video;return Un(n)?e:Ya(Ya({},e),{},{nextVideo:Xa(n)})}(e,t.payload.initiateParams));case"[DISCOVERY] show up next":return Ya(Ya({},e),{},{showUpNext:t.payload});case"[DISCOVERY] show skippable content":return Ya(Ya({},e),{},{showSkippableContent:t.payload});default:return e}}}),Qa=[],es=!1,ts=function e(){return function(t){return function(n){if(es)return Qa.push(n),null;es=!0;var r=t(n);return es=!1,Qa.length>0&&e()(t)(Qa.shift()),r}}},ns=function(e){var t=[];if(function(e){return!Un(e)&&!Un(e.enable_redux_debugging)&&e.enable_redux_debugging}(e)){var n=window&&window.__REDUX_DEVTOOLS_EXTENSION__&&window.__REDUX_DEVTOOLS_EXTENSION__();"function"===typeof n&&t.push(n)}var r=Et.apply(void 0,[wt(ua,ts)].concat(t));return vt(Ja,r)},rs=function(){function e(t){Ai()(this,e),f()(this,"playerVisibilitySubscriber",void 0),f()(this,"videoTagStatusSubscriber",void 0),f()(this,"shouldPlayIfLazyplay",!0),f()(this,"shouldPlayIfAutoplayWhenViewable",!0),f()(this,"videoPausedByObserver",!1),this.store=t,this.playerVisibilitySubscriber=null,this.videoTagStatusSubscriber=null,this.playAccordingToPlaybackMethod()}return Vi()(e,[{key:"lazyplayHandler",value:function(e){hn.playerVisibility(e)>=.5&&(this.playVideo(),this.shouldPlayIfLazyplay=!1)}},{key:"autoplayWhenViewableHandler",value:function(e){hn.playerVisibility(e)>=.5?this.playVideo():this.pauseVideo()}},{key:"onPlayerVisibilityChanged",value:function(e){var t=hn.playbackMethod(e);"lazyplay"===t&&this.shouldPlayIfLazyplay&&this.lazyplayHandler(e),"autoplay_when_viewable"===t&&this.shouldPlayIfAutoplayWhenViewable&&this.autoplayWhenViewableHandler(e)}},{key:"onVideoTagStatusChanged",value:function(e){var t=hn.videoTagStatus(e);"paused"!==t||this.videoPausedByObserver||(this.shouldPlayIfAutoplayWhenViewable=!1),"playing"===t&&(this.shouldPlayIfAutoplayWhenViewable=!0,this.videoPausedByObserver=!1)}},{key:"initiatePlayerVisibilitySubscriber",value:function(){this.playerVisibilitySubscriber=new ji(this.store,e.getPlayerVisibilityDependencies,this.onPlayerVisibilityChanged.bind(this))}},{key:"initiateVideoTagStatusSubscriber",value:function(){this.videoTagStatusSubscriber=new ji(this.store,e.getVideoTagStatusDependencies,this.onVideoTagStatusChanged.bind(this))}},{key:"playVideo",value:function(){var e=this.store,t=e.dispatch,n=e.getState;"idle"===hn.videoTagStatus(n())?on("play")(t):on("resume")(t)}},{key:"pauseVideo",value:function(){var e=this.store,t=e.dispatch,n=e.getState;"paused"!==hn.videoTagStatus(n())&&(this.videoPausedByObserver=!0,on("pause")(t))}},{key:"playAccordingToPlaybackMethod",value:function(){var e=this.store,t=e.dispatch,n=(0,e.getState)();switch(hn.playbackMethod(n)){case"autoplay":this.playVideo();break;case"lazyplay":this.initiatePlayerVisibilitySubscriber();break;case"autoplay_when_viewable":this.initiatePlayerVisibilitySubscriber(),this.initiateVideoTagStatusSubscriber();break;case"none":an(!1)(t)}}}],[{key:"getPlayerVisibilityDependencies",value:function(e){return[hn.playerVisibility(e)]}},{key:"getVideoTagStatusDependencies",value:function(e){return[hn.videoTagStatus(e)]}}]),e}(),is=function(){function e(t,n,r,i){var o=this;Ai()(this,e),f()(this,"videoStatusSubscriber",void 0),f()(this,"videoListSubscriber",void 0),f()(this,"mediaRequestSubscriber",void 0),f()(this,"playerVisibilitySubscriber",void 0),f()(this,"playbackMethodManager",void 0),f()(this,"store",void 0),f()(this,"loadContent",function(e,t,n,r){o.loadMedia(t,n,r).then(function(){o.playbackMethodManager=new rs(e)})}),f()(this,"loadMedia",function(e,t,n){var r=o.store,i=r.dispatch,a=r.getState,s=Dn.showTitle(a());if("semantic"===e){var u=pn.semanticOptions(a());return Na(u,s,n)(i)}return ka(t,s,n)(i)}),this.store=t,this.videoStatusSubscriber=new ji(t,e.getVideoStatusDependencies,this.onVideoStatusChanged.bind(this)),this.videoListSubscriber=new ji(t,e.getVideoListDependencies,this.onVideoListChanged.bind(this)),this.mediaRequestSubscriber=new ji(t,e.getMediaRequestDependencies,this.onMediaRequestChanged.bind(this)),this.playerVisibilitySubscriber=null,this.loadContent(t,r,n,i)}return Vi()(e,null,[{key:"createInstance",value:function(t,n,r,i){return new e(t,n,r,i)}}]),Vi()(e,[{key:"playNextVideo",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e),r=Cn.activeVideoIndex(e)+1;n.length>1&&r>=n.length&&(r=0),r<n.length&&(!function(e){e({type:"[CORE] reset player data time params"})}(t),La(r,n[r])(t),on("play")(t))}},{key:"playPreviousVideo",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e),r=Cn.activeVideoIndex(e);if(r>0){var i=r-1;La(i,n[i])(t),on("play")(t)}}},{key:"onVideoStatusChanged",value:function(e){"complete"===hn.videoTagStatus(e)&&this.playNextVideo(e)}},{key:"onVideoListChanged",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e);!jn(n)&&n.length>0&&La(0,n[0])(t)}},{key:"onMediaRequestChanged",value:function(e){var t=Cn.mediaRequest(e);switch(t.type){case"playNewVideo":this.loadMedia("specific",t.value);break;case"playNextVideo":this.playNextVideo(e);break;case"playPreviousVideo":this.playPreviousVideo(e)}}}],[{key:"getVideoStatusDependencies",value:function(e){return[hn.videoTagStatus(e)]}},{key:"getVideoListDependencies",value:function(e){return[Cn.videoList(e)]}},{key:"getMediaRequestDependencies",value:function(e){return[Cn.mediaRequest(e)]}}]),e}(),os=function e(t){var n=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"onDependencyFailure",function(e,t){console.log("onDependencyFailure",e,t);var r=n.store,i=r.dispatch,o=r.getState;switch(e){case"ima":"blocked"!==Fi.loadingImaStatus(o())&&Qn("error")(i);break;case"hls":er("error")(i)}}),f()(this,"onDependencyReady",function(e){var t=n.store.dispatch;switch(e){case"ima":Qn("success")(t);break;case"hls":er("success")(t)}}),this.store=t},as=function(e){return function(t){t({type:"[COMMON] set fullscreen",payload:e})}},ss=function(){function e(t,n){var r=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"videoTag",void 0),f()(this,"pendingFullscreenSubscriber",void 0),f()(this,"adStatusSubscriber",void 0),f()(this,"playerUniqId",void 0),f()(this,"onAdStatusChanged",function(e){var t=_i.adStatus(e),n=r.videoTag.webkitDisplayingFullscreen;"playing"===t&&Bn()&&n&&r.exitFullscreen(r.videoTag)}),f()(this,"isPlayerInFullscreen",function(){var e=document,t=Bn()?En(r.playerUniqId):bn(r.playerUniqId);return Un(e.fullscreenElement)?!Un(e.webkitFullscreenElement)&&0===e.webkitFullscreenElement.id.localeCompare(t):0===e.fullscreenElement.id.localeCompare(t)}),f()(this,"changePlayerWidth",function(e){r.videoTag.style.width=e?"100%":"auto"}),f()(this,"onFullscreenChanged",function(){var e=r.store.dispatch,t=r.isPlayerInFullscreen();r.changePlayerWidth(t),as(t)(e)}),f()(this,"onFullscreenChangedIos",function(){var e=r.store.dispatch,t=r.videoTag.webkitDisplayingFullscreen;t||on("resume")(e),r.changePlayerWidth(t),as(t)(e)}),f()(this,"onPendingFullscreenRequestChanged",function(e){var t=gn.pendingFullscreenRequest(e);"enter"===t?r.enterFullscreen(r.videoTag):"exit"===t&&r.exitFullscreen(r.videoTag)}),f()(this,"getFullScreenElement",function(e,t){var n=document.getElementById(bn(r.playerUniqId));return Bn()?t:e?document:n}),f()(this,"enterFullscreen",function(e){var t=r.getFullScreenElement(!1,e);Bn()?t.webkitEnterFullscreen():document.webkitExitFullscreen?t.webkitRequestFullscreen():document.webkitCancelFullScreen?t.webkitRequestFullScreen():document.mozCancelFullScreen?t.mozRequestFullScreen():document.msExitFullscreen&&t.msRequestFullscreen()}),f()(this,"exitFullscreen",function(e){var t=r.getFullScreenElement(!0,e);document.webkitExitFullscreen||Bn()?t.webkitExitFullscreen():document.webkitCancelFullScreen?t.webkitCancelFullScreen():document.mozCancelFullScreen?t.mozCancelFullScreen():document.msExitFullscreen&&t.msExitFullscreen()}),this.store=t,this.videoTag=document.getElementById(En(n)),this.playerUniqId=n,document.addEventListener("fullscreenchange",this.onFullscreenChanged.bind(this)),document.addEventListener("webkitfullscreenchange",this.onFullscreenChanged.bind(this)),Bn()&&(this.videoTag.addEventListener("webkitendfullscreen",this.onFullscreenChangedIos.bind(this)),this.videoTag.addEventListener("webkitbeginfullscreen",this.onFullscreenChangedIos.bind(this))),this.pendingFullscreenSubscriber=new ji(t,e.getPendingFullscreenDependencies,this.onPendingFullscreenRequestChanged.bind(this)),this.adStatusSubscriber=new ji(t,e.getAdStatusDependencies,this.onAdStatusChanged.bind(this))}return Vi()(e,null,[{key:"createInstance",value:function(t,n){return new e(t,n)}}]),Vi()(e,null,[{key:"getPendingFullscreenDependencies",value:function(e){return[gn.pendingFullscreenRequest(e)]}},{key:"getAdStatusDependencies",value:function(e){return[_i.adStatus(e)]}}]),e}();function us(e,t){var n=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter(function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable})),n.push.apply(n,r)}return n}function cs(e){for(var t=1;t<arguments.length;t++){var n=null!=arguments[t]?arguments[t]:{};t%2?us(Object(n),!0).forEach(function(t){f()(e,t,n[t])}):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(n)):us(Object(n)).forEach(function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(n,t))})}return e}var ls,ds=function(e){return function(e){return e&&window.monti.playerConfigs&&window.monti.playerConfigs[e]}(e)?function(e){return window.monti.playerConfigs[e]}(e):window.monti.playerConfigs?window.monti.playerConfigs&&window.monti.playerConfigs[Object.keys(window.monti.playerConfigs)[0]]:null},ps=function e(t){var n=this;Ai()(this,e),f()(this,"videoTag",void 0),f()(this,"isBufferError",void 0),f()(this,"hls",void 0),f()(this,"hlsSetup",function(e,t,r,i){n.initiateHls(e),n.loadHlsSource(e,t,r,i)}),f()(this,"detachMedia",function(){Un(n.hls)||(n.hls.detachMedia(),n.hls.destroy(),n.hls=null)}),f()(this,"initiateHls",function(e){n.hls=new e,n.hls.attachMedia(n.videoTag)}),f()(this,"loadHlsSource",function(e,t,r,i){n.hls.on(e.Events.MEDIA_ATTACHED,function(){n.hls.loadSource(t)}),n.hls.on(e.Events.ERROR,function(t,o){n.mapHlsToErrors(e,o,i),t.details===e.ErrorDetails.BUFFER_STALLED_ERROR&&(r(!0),n.isBufferError=!0)}),n.hls.on(e.Events.FRAG_BUFFERED,function(){n.isBufferError&&(r(!1),n.isBufferError=!1)})}),f()(this,"mapHlsToErrors",function(e,t,r){if(t.fatal)switch(t.type){case e.ErrorTypes.NETWORK_ERROR:r(Xn.GENERAL_ERROR),n.hls.startLoad();break;case e.ErrorTypes.MEDIA_ERROR:r(Xn.GENERAL_ERROR),n.hls.recoverMediaError();break;default:r(Xn.GENERAL_ERROR),n.hls.destroy()}}),this.hls=void 0,this.videoTag=t,this.isBufferError=!1},fs=function e(){var t=this;Ai()(this,e),f()(this,"videoStreaming",void 0),f()(this,"hlsLibrarySetup",function(e,n,r,i){Un(t.videoStreaming)||t.videoStreaming.detachMedia(),t.videoStreaming=new ps(e),t.videoStreaming.hlsSetup(ls,n,r,i)})};f()(fs,"shouldLoadVideoStreamingSrcDirectly",function(e,t,n){return"no-need"===n&&!(""===e.canPlayType("application/vnd.apple.mpegurl"))}),f()(fs,"shouldUseHlsLibrary",function(e,t){return"success"===t&&(ls=void 0!==window.Hls?Hls:mmHls).isSupported()}),f()(fs,"isValidHlsUrl",function(e){return!Un(e)&&!e.includes(".mp4")}),f()(fs,"suitableVideoSource",function(e,t,n){return fs.isValidHlsUrl(t)?fs.shouldUseHlsLibrary(t,n)?"m3u8 with hls":fs.shouldLoadVideoStreamingSrcDirectly(e,t,n)?"m3u8 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{"is_conflicting_with_other_jw_players":false,"programmatic_play_with_sound_on_desktop":false,"referrer_id":"af93e181-b289-0560-a2bf-808e93bb05bc","width":"100","comscore_publisher_id":"18120612","monetization":{"ad_type":"static_tag","continue_content_play_while_waiting_for_ad":false,"strategy":"on_player_load","ad_request_timeout":"10000","midrolls":{"on":[0]},"vpaid_mode":"ENABLED","ad_tag":"https://pubads.g.doubleclick.net/gampad/ads?sz=400x300|640x480|480x270|640x360&iu=/175840252/MMPlus/smithsonianmag/Video&impl=s&gdfp_req=1&env=vp&output=vast&unviewed_position_start=1&url=##REFERRER_URL_UNESC##&description_url=##DESCRIPTION_URL_UNESC##&correlator=##CACHEBUSTER##&cust_params=mm_midroll%3D##MIDROLL_ORDER##%26video_ID%3D##VIDEO_ID##"},"sponsorship":false,"player_identifier":"mplayer","recommendation_id":null,"brand_color":"#FF9900","powered_by_strip":true,"platform":"buffy","type":"video","config_name":"MM+ | Smithsonianmag | Podding","player_id":"3v9g2u2f","playlist_id":"fSkmeWKF","playback_method":"autoplay","anchor_viewability_method":"none","player_version":"v4","playlist_type":"semantic","semantic_options":{"scan_images_on_page":true,"scanned_element":"","tags":"geogrophy,nature,animals,habitat,outdoors,science,history","minimum_date_factor":30,"scanned_element_type":"tag","scoped_keywords":"mentalfloss","promoted_videos":[]},"script_destination":"mm","publisher_contribution":"floor8","general_script_description":"","brand_logo":"","brand_logo_click_url":"","next_video":"none","uniq_key":"af93e181-b289-0560-a2bf-808e93bb05bc","content_id":"fSkmeWKF","content_type":"semantic"})); Finland has vastly improved in reading, math and science literacy over the past decade in large part because its teachers are trusted to do whatever it takes to turn young lives around. This 13-year-old, Besart Kabashi, received something akin to royal tutoring. “I took Besart on that year as my private student,” Louhivuori told me in his office, which boasted a Beatles “Yellow Submarine” poster on the wall and an electric guitar in the closet. When Besart was not studying science, geography and math, he was parked next to Louhivuori’s desk at the front of his class of 9- and 10-year- olds, cracking open books from a tall stack, slowly reading one, then another, then devouring them by the dozens. By the end of the year, the son of Kosovo war refugees had conquered his adopted country’s vowel-rich language and arrived at the realization that he could, in fact, learn. Years later, a 20-year-old Besart showed up at Kirkkojarvi’s Christmas party with a bottle of Cognac and a big grin. “You helped me,” he told his former teacher. Besart had opened his own car repair firm and a cleaning company. “No big fuss,” Louhivuori told me. “This is what we do every day, prepare kids for life.” This tale of a single rescued child hints at some of the reasons for the tiny Nordic nation’s staggering record of education success, a phenomenon that has inspired, baffled and even irked many of America’s parents and educators. Finnish schooling became an unlikely hot topic after the 2010 documentary film Waiting for “Superman” contrasted it with America’s troubled public schools. “Whatever it takes” is an attitude that drives not just Kirkkojarvi’s 30 teachers, but most of Finland’s 62,000 educators in 3,500 schools from Lapland to Turku—professionals selected from the top 10 percent of the nation’s graduates to earn a required master’s degree in education. Many schools are small enough so that teachers know every student. If one method fails, teachers consult with colleagues to try something else. They seem to relish the challenges. Nearly 30 percent of Finland’s children receive some kind of special help during their first nine years of school. The school where Louhivuori teaches served 240 first through ninth graders last year; and in contrast with Finland’s reputation for ethnic homogeneity, more than half of its 150 elementary-level students are immigrants—from Somalia, Iraq, Russia, Bangladesh, Estonia and Ethiopia, among other nations. “Children from wealthy families with lots of education can be taught by stupid teachers,” Louhivuori said, smiling. “We try to catch the weak students. It’s deep in our thinking.” Advertisement scroll for more The transformation of the Finns’ education system began some 40 years ago as the key propellent of the country’s economic recovery plan. Educators had little idea it was so successful until 2000, when the first results from the Programme for International Student Assessment (PISA), a standardized test given to 15-year-olds in more than 40 global venues, revealed Finnish youth to be the best young readers in the world. Three years later, they led in math. By 2006, Finland was first out of 57 countries (and a few cities) in science. In the 2009 PISA scores released last year, the nation came in second in science, third in reading and sixth in math among nearly half a million students worldwide. “I’m still surprised,” said Arjariita Heikkinen, principal of a Helsinki comprehensive school. “I didn’t realize we were that good.” In the United States, which has muddled along in the middle for the past decade, government officials have attempted to introduce marketplace competition into public schools. In recent years, a group of Wall Street financiers and philanthropists such as Bill Gates have put money behind private-sector ideas, such as vouchers, data-driven curriculum and charter schools, which have doubled in number in the past decade. President Obama, too, has apparently bet on compe­tition. His Race to the Top initiative invites states to compete for federal dollars using tests and other methods to measure teachers, a philosophy that would not fly in Finland. “I think, in fact, teachers would tear off their shirts,” said Timo Heikkinen, a Helsinki principal with 24 years of teaching experience. “If you only measure the statistics, you miss the human aspect.”

      The facts show that America has it all wrong in putting to much emphasis on national "data-driven" competition. These approaches take away from the unique aspects of each child.

    2. t was the end of term at Kirkkojarvi Comprehensive School in Espoo, a sprawling suburb west of Helsinki, when Kari Louhivuori, a veteran teacher and the school’s principal, decided to try something extreme—by Finnish standards. One of his sixth-grade students, a Kosovo-Albanian boy, had drifted far off the learning grid, resisting his teacher’s best efforts. The school’s team of special educators—including a social worker, a nurse and a psychologist—convinced Louhivuori that laziness was not to blame. 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t="midroll".concat(e.midrollNumber);return r.adTagGenerator.generate(t,e.mediaId)}),f()(this,"generatePrerollTag",function(e,t){var n="preroll".concat(t);return r.adTagGenerator.generate(n,e)}),f()(this,"onAdTimeReached",function(){r.monetization.onMidrollAdOpportunity()}),f()(this,"onPreAdTimeReached",function(e){r.onPreMidrollAdOpportunity(e)}),f()(this,"onSeekToAdOpportunity",function(e){r.onPreMidrollAdOpportunity(e)}),f()(this,"isMidrollAlreadyRequested",function(e){return e.midrollNumber===r.lastRequestedMidroll.midrollNumber&&e.mediaId===r.lastRequestedMidroll.mediaId&&e.midrollTime===r.lastRequestedMidroll.midrollTime}),f()(this,"onPreMidrollAdOpportunity",function(e){if(Un(r.lastRequestedMidroll)||!r.isMidrollAlreadyRequested(e)){r.lastRequestedMidroll=e;var t=r.generateMidrollTag(e);r.monetization.onPreMidrollAdOpportunity(e,t)}}),f()(this,"onPrerollReached",function(e,t){var n=r.generatePrerollTag(e,t);r.monetization.onPrerollAdOpportunity(n)}),f()(this,"onSeekedWhileAdInProgress",function(){r.monetization.onMidrollAdOpportunity()});var i=t.getState;this.monetization=n,this.videoTimeSubscriber=new qi(t,this),this.videoSeekSubscriber=new zi(t,this),this.prerollScheduler=new Gi(t,this);var o=_i.adTagUrlTemplate(i());this.adTagGenerator=new Wi(o)},Yi=function(){function e(){Ai()(this,e)}return Vi()(e,null,[{key:"generateAdRequest",value:function(e,t,n){var r=new google.ima.AdsRequest;return r.adTagUrl=e,Fn()||r.setAdWillPlayMuted(t),r.vastLoadTimeout=n,r}}]),e}(),Zi=function(e){return function(t){t({type:"[MONETIZATION] change ad status",payload:e})}},Xi=function(e){return function(t){t({type:"[COMMON] set pending video status",payload:{pendingStatusObject:{type:e,value:""}}})}},Ji=function(e){return function(t){t({type:"[MONETIZATION] change loading ad status",payload:e})}},Qi=function(e){return function(t){t({type:"[MONETIZATION] update ad muted",payload:e})}},eo=function(e){return function(t){t({type:"[MONETIZATION] change ad volume",payload:e})}},to=function e(t,n,r,i,o){var a=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"IMAAdManager",void 0),f()(this,"adsLoader",void 0),f()(this,"adDisplayContainer",void 0),f()(this,"adVideoElement",void 0),f()(this,"videoPlayerElement",void 0),f()(this,"adContainerElement",void 0),f()(this,"anchorStatusStoreSubscriber",void 0),f()(this,"totalAdRequestMadeAmount",0),f()(this,"registerForWindowResize",function(){var e=zn(a.onResize.bind(a),80);window.addEventListener("resize",e)}),f()(this,"initMutationObserver",function(e){new MutationObserver(a.onResize).observe(e,{attributes:!0,childList:!1,subtree:!1})}),f()(this,"loadNewAd",function(e,t){var n=a.store.dispatch;a.clearOldAdManagerIfExist();var r=a.createAdRequest(e);try{a.validateAdRequestCorrectness(r),a.adsLoader.requestAds(r),function(e,t){return function(n){n({type:"[MONETIZATION] change ad 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google.ima.AdsLoader(t),a.adsLoader.getSettings().setDisableCustomPlaybackForIOS10Plus(!0),a.adsLoader.getSettings().setVpaidMode(google.ima.ImaSdkSettings.VpaidMode[n]),a.adsLoader.addEventListener(google.ima.AdsManagerLoadedEvent.Type.ADS_MANAGER_LOADED,a.onIMAAdsManagerLoaded.bind(a),!1,a),a.adsLoader.addEventListener(google.ima.AdErrorEvent.Type.AD_ERROR,a.onAdError.bind(a),!1,a)}),f()(this,"createAdRequest",function(e){var t=a.store.getState,n=gn.muted(t()),r=bi.adRequestTimeout(t());return Yi.generateAdRequest(e,n,r)}),f()(this,"validateAdRequestCorrectness",function(e){e&&e.adTagUrl&&decodeURIComponent(e.adTagUrl.replace(/\+/g," "))}),f()(this,"getLoadingError",function(e){var t=function(){return"bad ad request ".concat(JSON.stringify(e))};return{getError:function(){return{getMessage:t}}}}),f()(this,"getPlayAdError",function(e){var t=function(){return"play ad error: 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t=a.store,n=t.getState,r=t.dispatch,i=gn.volume(n());Bn()||gn.muted(n())?(e.setVolume(0),Qi(!0)(r)):(e.setVolume(gn.volume(n())),eo(i)(r),Qi(!1)(r))}),f()(this,"createIMAAdManager",function(t){a.IMAAdManager=t.getAdsManager(a.adVideoElement,e.getAdsRenderingSettings()),a.setAdVolume(a.IMAAdManager)}),f()(this,"registerToAdManagerEvents",function(){a.IMAAdManager.addEventListener(google.ima.AdErrorEvent.Type.AD_ERROR,a.onAdError),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.CONTENT_PAUSE_REQUESTED,a.onContentPauseRequested),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.CONTENT_RESUME_REQUESTED,a.onContentResumeRequested),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.STARTED,a.onAdStarted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.IMPRESSION,a.onAdImpression),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.SKIPPED,a.onAdSkipped),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.COMPLETE,a.onAdCompleted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.PAUSED,a.onAdPaused),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.RESUMED,a.onAdStarted),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.AD_PROGRESS,a.onAdProgressChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.VOLUME_CHANGED,a.onVolumeChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.VOLUME_MUTED,a.onAdVolumeMutedChanged),a.IMAAdManager.addEventListener(google.ima.AdEvent.Type.ALL_ADS_COMPLETED,a.onAdCompleted)}),f()(this,"onIMAAdsManagerLoaded",function(e){var t=a.store.dispatch;a.createIMAAdManager(e),a.registerToAdManagerEvents(),Zi("loaded")(t)}),f()(this,"onAdError",function(e){var t=a.store.dispatch;!function(e){return function(t){t({type:"[MONETIZATION] change ad error",payload:e})}}(e.getError().getMessage())(t),Ji(!1),a.continuePlayingContent()}),f()(this,"onAdImpression",function(e){var t=a.store.dispatch,n=!e.getAd().g.vpaid;a.setPodInfo(e),function(e){e({type:"[MONETIZATION] increase ad impression counter"})}(t),function(e){return function(t){t({type:"[MONETIZATION] update is vast ad",payload:e})}}(n)(t)}),f()(this,"onVolumeChanged",function(e){var t=a.store.dispatch;eo(e.target.getVolume())(t)}),f()(this,"onAdVolumeMutedChanged",function(e){var t=a.store.dispatch;0===e.target.getVolume()?Qi(!0)(t):Qi(!1)(t)}),f()(this,"continuePlayingContent",function(){var e=a.store,t=e.getState,n=e.dispatch,r=hn.videoTagStatus(t());Xi("idle"===r?"play":"resume")(n)}),f()(this,"stopPlayingContent",function(){var e=a.store.dispatch;Xi("pause")(e)}),f()(this,"onContentPauseRequested",function(){a.stopPlayingContent()}),f()(this,"onContentResumeRequested",function(){a.continuePlayingContent()}),f()(this,"onAdPaused",function(){var e=a.store.dispatch;Zi("paused")(e)}),f()(this,"setPodInfo",function(e){var t=e&&e.getAd()&&e.getAd().getAdPodInfo();if(!Un(t)){var n=a.store.dispatch;!function(e,t){return function(n){n({type:"[MONETIZATION] change pod info",payload:{slotNumber:e,podNumber:t}})}}(t.getAdPosition(),a.totalAdRequestMadeAmount)(n)}}),f()(this,"onAdStarted",function(){var e=a.store,t=e.dispatch,n=e.getState,r=gn.volume(n());Zi("playing")(t),0===a.IMAAdManager.getVolume()?a.IMAAdManager.setVolume(0):window.shouldPlayAdRule||a.IMAAdManager.setVolume(r),a.onResize()}),f()(this,"onAdCompleted",function(){var e=a.store.dispatch;Zi("completed")(e)}),f()(this,"onAdSkipped",function(){var e=a.store.dispatch;Zi("skipped")(e)}),f()(this,"onResize",function(){Un(a.IMAAdManager)||(a.IMAAdManager.resize(a.videoPlayerElement.clientWidth,a.videoPlayerElement.clientHeight,google.ima.ViewMode.NORMAL),a.adContainerElement.style.height="".concat(a.videoPlayerElement.clientHeight,"px"))}),f()(this,"onAdProgressChanged",function(e){var t,n,r=a.store,i=r.dispatch,o=r.getState,s=e.getAdData().currentTime,u=e.getAdData().duration,c=_i.adDuration(o());(t=s,function(e){e({type:"[MONETIZATION] change ad current time",payload:t})})(i),c!==u&&(n=u,function(e){e({type:"[MONETIZATION] change ad duration",payload:n})})(i)}),f()(this,"onAnchorStatusChanged",function(){var e=a.store.getState;"processing"!==Pr(e())&&a.onResize()}),f()(this,"changeAdVolume",function(e){Un(a.IMAAdManager)||a.IMAAdManager.setVolume(e)}),f()(this,"changeAdMuted",function(e,t){Un(a.IMAAdManager)||(t?a.IMAAdManager.setVolume(0):a.IMAAdManager.setVolume(e))}),f()(this,"changeAdStatus",function(e){Un(a.IMAAdManager)||("playing"===e&&a.IMAAdManager.resume(),"paused"===e&&a.IMAAdManager.pause())});var s=t.getState;this.store=t,this.adVideoElement=r,this.videoPlayerElement=i,this.adContainerElement=n,this.adDisplayContainer=new google.ima.AdDisplayContainer(n,r),this.createAdLoader(s(),this.adDisplayContainer),this.adDisplayContainer.initialize(),this.anchorStatusStoreSubscriber=new ji(t,e.getAnchorDependencies,this.onAnchorStatusChanged.bind(this)),this.registerForWindowResize(),this.initMutationObserver(o)};f()(to,"getAdsRenderingSettings",function(){var e=new google.ima.AdsRenderingSettings;return e.restoreCustomPlaybackStateOnAdBreakComplete=!0,e.enablePreloading=!1,e.uiElements=[],e.loadVideoTimeout=15e3,e}),f()(to,"getAnchorDependencies",function(e){return[Pr(e)]});var no=function e(t,n,r,i,o,a){var s=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"playerId",void 0),f()(this,"adScheduler",void 0),f()(this,"adHandler",void 0),f()(this,"imaLoadingStatusSubscriber",void 0),f()(this,"adStatusSubscriber",void 0),f()(this,"videoTagStatusSubscriber",void 0),f()(this,"adContainer",void 0),f()(this,"adVideoElement",void 0),f()(this,"videoPlayerElement",void 0),f()(this,"playerContainer",void 0),f()(this,"pendingMidrollAdPlay",!1),f()(this,"pendingPrerollAdPlay",!1),f()(this,"pendingPrerollAdTag",null),f()(this,"pendingMidrollNumber",null),f()(this,"pendingAdStatusStoreSubscriber",void 0),f()(this,"adMutedStoreSubscriber",void 0),f()(this,"adVolumeStoreSubscriber",void 0),f()(this,"onMidrollAdOpportunity",function(){var e=s.store,t=e.dispatch,n=e.getState,r=_i.adStatus(n()),i=bi.continuePlayingWhileWaitingForAd(n());"loaded"===r?s.playAd(!0):"requested"===r&&(s.pendingMidrollAdPlay=!0,i||(Xi("pause")(t),Ji(!0)(t))),function(e){e({type:"[MONETIZATION] increase ad Opportunity counter"})}(t)}),f()(this,"onPrerollAdOpportunity",function(e){var t=s.store,n=t.getState,r=t.dispatch,i=Fi.loadingImaStatus(n());Un(s.adHandler)?"loading"!==i&&""!==i||(Ji(!0)(r),s.pendingPrerollAdPlay=!0,s.pendingPrerollAdTag=e):(s.pendingPrerollAdPlay=!0,Ji(!0)(r),s.adHandler.loadNewAd(e,"preroll"))}),f()(this,"onPreMidrollAdOpportunity",function(e,t){Un(s.adHandler)||(e.currentTime>=e.midrollTime&&(s.pendingMidrollAdPlay=!0),s.pendingMidrollNumber=e.midrollNumber,s.adHandler.loadNewAd(t,"midroll"))}),f()(this,"hasPendingAd",function(){return s.hasPendingMidrollAdPlay()||s.hasPendingPrerollAdPlay()}),f()(this,"onAdStatusChanged",function(e){var t=s.store.dispatch,n=_i.adStatus(e);"completed"===n&&Ji(!1)(t);var r=bi.continuePlayingWhileWaitingForAd(e),i=_i.loadingAd(e);"playing"!==n&&"error"!==n||r||!i||Ji(!1)(t),s.hasPendingAd()&&"loaded"===n?s.playAd(s.hasPendingMidrollAdPlay()):s.hasPendingAd()&&"error"===n?(Ji(!1),s.clearPendingMidroll(),s.clearPendingPreroll()):Hi(n)||(Ji(!1),function(e){e({type:"[MONETIZATION] clear ad data"})}(t))}),f()(this,"clearPendingMidroll",function(){s.pendingMidrollNumber=null,s.pendingMidrollAdPlay=!1}),f()(this,"clearPendingPreroll",function(){s.pendingPrerollAdPlay=!1,s.pendingPrerollAdTag=null}),f()(this,"onVideoTagStatusChanged",function(e){"complete"===hn.videoTagStatus(e)&&function(e){e({type:"[MONETIZATION] clear played midrolls"})}(s.store.dispatch)}),f()(this,"hasPendingMidrollAdPlay",function(){return s.pendingMidrollAdPlay}),f()(this,"hasPendingPrerollAdPlay",function(){return s.pendingPrerollAdPlay}),f()(this,"playAd",function(e){var t,n=s.store.dispatch,r=s.adHandler.playAd();e?((t=s.pendingMidrollNumber,function(e){e({type:"[MONETIZATION] add 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Ki(t,this),this.adStatusSubscriber=new ji(t,e.getAdStatusDependencies,this.onAdStatusChanged.bind(this)),this.videoTagStatusSubscriber=new ji(t,e.getVideoTagStatusDependencies,this.onVideoTagStatusChanged.bind(this)),e.canUseIMA(u())?this.adHandler=new to(t,r,i,o,a):this.imaLoadingStatusSubscriber=new ji(t,e.getIMALoadingStatusDependencies,this.onIMALoadingStatusChanged.bind(this)),this.pendingAdStatusStoreSubscriber=new ji(t,e.getPendingAdStatusDependencies,this.onPendingAdStatusChanged.bind(this)),this.adMutedStoreSubscriber=new ji(t,e.getAdMutedDependencies,this.onAdMutedChanged.bind(this)),this.adVolumeStoreSubscriber=new ji(t,e.getAdVolumeDependencies,this.onAdVolumeChanged.bind(this))};f()(no,"getAdStatusDependencies",function(e){return[_i.adStatus(e)]}),f()(no,"getVideoTagStatusDependencies",function(e){return[hn.videoTagStatus(e)]}),f()(no,"getIMALoadingStatusDependencies",function(e){return[Fi.loadingImaStatus(e)]}),f()(no,"canUseIMA",function(e){return"success"===Fi.loadingImaStatus(e)}),f()(no,"getPendingAdStatusDependencies",function(e){return[_i.pendingAdStatus(e)]}),f()(no,"getAdMutedDependencies",function(e){return[_i.adMuted(e)]}),f()(no,"getAdVolumeDependencies",function(e){return[_i.adVolume(e)]});var ro=function(e,t){!function(e,t){var n=document.getElementById(vn(t));B(b(Li,{store:e,playerId:t}),n)}(e,t);var n=function(e){var t=Ri(e);return document.getElementById(t)}(t),r=function(e){var t=Bn()?Di(e):En(e);return document.getElementById(t)}(t),i=function(e){var t=En(e);return document.getElementById(t)}(t),o=function(e){var t=bn(e);return document.getElementById(t)}(t);return new no(e,t,n,r,i,o)},io=n(4),oo=n.n(io),ao=n(7),so=n.n(ao),uo=function(){function e(){Ai()(this,e),f()(this,"duration",void 0),f()(this,"position",void 0),f()(this,"previousPosition",void 0),f()(this,"loadTime",void 0),f()(this,"adOrder",void 0),f()(this,"adType",void 0),f()(this,"adDuration",void 0),f()(this,"errorMessage",void 0),f()(this,"adPodNumber",void 0),f()(this,"adSlotNumber",void 0)}return Vi()(e,[{key:"setDuration",value:function(e){return this.duration=e,this}},{key:"setPosition",value:function(e){return this.position=e,this}},{key:"setPreviousPosition",value:function(e){return this.previousPosition=e,this}},{key:"setLoadTime",value:function(e){return this.loadTime=e,this}},{key:"setAdOrder",value:function(e){return this.adOrder=e,this}},{key:"setAdType",value:function(e){return this.adType=e,this}},{key:"setAdDuration",value:function(e){return this.adDuration=e,this}},{key:"setErrorMessage",value:function(e){return this.errorMessage=e,this}},{key:"setAdPodNumber",value:function(e){return this.adPodNumber=e,this}},{key:"setAdSlotNumber",value:function(e){return this.adSlotNumber=e,this}},{key:"build",value:function(){var e=[];return jn(this.position)||e.push("video current position=".concat(Hn(this.position),"sec")),jn(this.duration)||e.push("video duration time=".concat(Hn(this.duration),"sec")),jn(this.loadTime)||e.push("video load time=".concat(this.loadTime,"milliseconds")),jn(this.previousPosition)||e.push("previous position=".concat(Hn(this.previousPosition),"sec")),jn(this.adOrder)||e.push("ad order=".concat(this.adOrder)),jn(this.adType)||e.push("ad type=".concat(this.adType)),jn(this.adDuration)||e.push("ad duration=".concat(Hn(Number(this.adDuration)),"sec")),jn(this.adPodNumber)||e.push("pod number=".concat(this.adPodNumber)),jn(this.adSlotNumber)||e.push("slot number=".concat(this.adSlotNumber)),jn(this.errorMessage)||e.push("error message=".concat(this.errorMessage)),e.join(";")}}]),e}(),co="mmPlus GTM data ready to GA",lo="mmPlus GTM event to GA",po={EMBED:"vplayer video player embed",FIRST_PLAY:"vplayer video first play",COMPLETION_25_PERCENTAGE:"vplayer video 25% complete",COMPLETION_50_PERCENTAGE:"vplayer video 50% complete",COMPLETION_75_PERCENTAGE:"vplayer video 75% complete",COMPLETION_90_PERCENTAGE:"vplayer video 90% complete",AD_BLOCK:"vplayer video ad block",AD_REQUEST:"vplayer video ad request",AD_IMPRESSION:"vplayer video ad impression",AD_ERROR:"vplayer video ad error",AD_VIEWABLE_IMPRESSION:"vplayer video ad viewable impression",AD_COMPLETE:"vplayer video ad complete",AD_SKIP:"vplayer video ad skip",AD_PAUSE:"vplayer video ad pause",VIDEO_COMPLETE:"vplayer video complete",FULLSCREEN_ON:"vplayer video fullscreen on",FULLSCREEN_OFF:"vplayer video fullscreen off",SEEK:"vplayer video position seeked",VIDEO_MUTE:"vplayer video mute",VIDEO_UNMUTE:"vplayer video unmute",CONTROLS_MUTE_OR_UNMUTE:"controls 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t=gn.fullscreen(e),n=hn.currentVideoTimeFragment(e),i=(new uo).setPosition(n).build(),o=t?po.FULLSCREEN_ON:po.FULLSCREEN_OFF;r.analyticsEventsCallbacks.onEvent(o,i)}),this.store=t,this.analyticsEventsCallbacks=n,this.videoMuteSubscriber=new ji(t,e.getVideoMuteDependencies,this.onMuteStateChanged.bind(this)),this.videoFullscreenSubscriber=new ji(t,e.getVideoFullscreenDependencies,this.onFullsScreenStateChanged.bind(this))};f()(yo,"getVideoMuteDependencies",function(e){return[gn.muted(e)]}),f()(yo,"getVideoFullscreenDependencies",function(e){return[gn.fullscreen(e)]});var go=n(3),vo=n.n(go),mo=n(8),bo=n.n(mo),Oo=n(9),_o=n.n(Oo),So=n(5),Eo=n.n(So);n(20);var wo={root:null,threshold:.5,rootMargin:"0px"},Po=function(){function e(t,n,r){Ai()(this,e),f()(this,"store",void 0),f()(this,"observableElement",void 0),f()(this,"callback",void 0),f()(this,"isViewableTimeoutHandler",null),f()(this,"observer",void 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The initial state may not be undefined, but can be null.')})}(n)}catch(s){o=s}return function(e,t){if(void 0===e&&(e={}),o)throw o;for(var r=!1,i={},s=0;s<a.length;s++){var u=a[s],c=n[u],l=e[u],d=c(l,t);if("undefined"===typeof d){var p=mt(u,t);throw new Error(p)}i[u]=d,r=r||d!==l}return(r=r||a.length!==Object.keys(e).length)?i:e}}({dependenciesLoadingStatus:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:da,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] update hls status":return la(la({},e),{},{loadingHLSStatus:t.payload});case"[CORE] update ima status":return la(la({},e),{},{loadingImaStatus:t.payload});default:return e}},playerData:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:ha,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":var n=t.payload;return fa({},function(e,t,n){var r=t.playback_method,i=t.player_id;return fa(fa({},e),{},{playbackMethod:Un(r)?e.playbackMethod:r,playerId:Un(i)?e.playerId:i,playerInstanceUniqId:n,playerMode:Fn()?"mobile":"desktop"})}(e,n.initiateParams,n.playerInstanceUniqId));case"[CORE] reset player data time params":return fa(fa({},e),{},{currentVideoTimeFragment:0,currentVideoBufferedTime:0,currentVideoDuration:0,currentVideoTime:0});case"[COMMON] set mute video":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{muted:t.payload})});case"[COMMON] set volume":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{volume:t.payload})});case"[COMMON] change selected settings category":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{selectedSettingsCategory:t.payload})});case"[COMMON] change settings speed":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{speed:t.payload})});case"[COMMON] change settings quality":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{quality:t.payload})});case"[COMMON] set fullscreen":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{fullscreen:fa(fa({},e.playerSettings.fullscreen),{},{isFullscreenOn:t.payload,pendingFullscreenRequest:""})})});case"[COMMON] set fullscreen request":return fa(fa({},e),{},{playerSettings:fa(fa({},e.playerSettings),{},{fullscreen:fa(fa({},e.playerSettings.fullscreen),{},{pendingFullscreenRequest:t.payload})})});case"[COMMON] set pending video status":var r=t.payload.pendingStatusObject;return fa(fa({},e),{},{pendingVideoTagStatus:fa({},r)});case"[COMMON] set player mode":return fa(fa({},e),{},{playerMode:t.payload});case"[CORE] update video current fragment position":return fa(fa({},e),{},{currentVideoTimeFragment:t.payload});case"[CORE] update video current position":return fa(fa({},e),{},{currentVideoTime:t.payload});case"[CORE] update video current buffered time":return fa(fa({},e),{},{currentVideoBufferedTime:t.payload});case"[CORE] update video current duration":return fa(fa({},e),{},{currentVideoDuration:t.payload});case"[CORE] change video tag status":return fa(fa({},e),{},{videoTagStatus:t.payload});case"[CORE] update player visibility":return fa(fa({},e),{},{playerVisibility:t.payload});case"[CORE] update placeholder visibility":return fa(fa({},e),{},{playerPlaceholderVisibility:t.payload});case"[CORE] change loading player status":return fa(fa({},e),{},{loadingPlayer:t.payload});case"[COMMON] show black screen with loader":return fa(fa({},e),{},{loader:fa(fa({},e.loader),{},{showBlackScreen:t.payload})});case"[CORE] set player size":return fa(fa({},e),{},{playerSize:t.payload});case"[COMMON] set error message":return fa(fa({},e),{},{errorMessage:t.payload});default:return e}},brandingData:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:va,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return ga({},function(e,t){var n=t.powered_by_strip,r=t.brand_logo,i=t.brand_logo_click_url,o=t.brand_color;return ga(ga({},e),{},{showVoltaxLogo:Un(n)?e.showVoltaxLogo:n,brandingLogoSrc:Un(r)?e.brandingLogoSrc:r,brandingLogoUrl:Un(i)?e.brandingLogoUrl:i,brandingColor:Un(o)?e.brandingColor:o})}(e,t.payload.initiateParams));default:return e}},anchorOptions:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Oa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return ba({},function(e,t){var n=t.anchor_options;if(!Un(n)){var r=n.anchoring_appearance,i=n.can_close,o=n.closable_ad,a=n.close_after,s=n.continue_streaming,u=n.orientation,c=n.margins,l=n.sticky_below_class_name,d=n.width,p=Un(c)?e.margins:{top:Number.isInteger(c.top)?c.top:e.margins.top,bottom:Number.isInteger(c.bottom)?c.bottom:e.margins.bottom,left:Number.isInteger(c.left)?c.left:e.margins.left,right:Number.isInteger(c.right)?c.right:e.margins.right};return ba(ba({},e),{},{anchoringAppearance:r||e.anchoringAppearance,canClose:Un(i)?e.canClose:i,orientation:Un(u)?e.orientation:u,closableAd:Un(o)?e.closableAd:o,closeAfter:Un(a)?e.closeAfter:a,continueStreaming:Un(s)?e.continueStreaming:s,stickyBelowClassName:Un(l)?e.stickyBelowClassName:l,width:Un(d)?e.width:d,margins:p,anchorData:ba(ba({},e.anchorData),{},{anchorEnabled:!0})})}return e}(e,t.payload.initiateParams));case"[COMMON] set anchor enable":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorEnabled:t.payload})});case"[ANCHOR] update is anchor status":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorStatus:t.payload})});case"[COMMON] set anchor disabled by user":return ba(ba({},e),{},{anchorData:ba(ba({},e.anchorData),{},{anchorDisabledByUser:t.payload})});default:return e}},monetization:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:wa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Sa({},function(e,t){var n=t.monetization;if(Un(n))return e;var r=n.ad_tag,i=n.ad_type,o=n.vpaid_mode,a=n.ad_request_timeout,s=n.continue_content_play_while_waiting_for_ad,u=n.midrolls,c=u&&u.on&&u.on.sort(Wn),l=Un(s)?e.continuePlayingWhileWaitingForAd:s,d=c?c.indexOf(0):-1,p=-1!==d&&!l;return p&&(c=c.splice(d,1)),Sa(Sa({},e),{},{midrolls:Sa(Sa({},e.midrolls),{},{every:u&&u.every,on:c}),prerollEnabled:p,adRequestTimeout:Un(a)?e.adRequestTimeout:parseInt(a,10),vpaidMode:Un(o)?e.vpaidMode:o,continuePlayingWhileWaitingForAd:l,adsData:Sa(Sa({},e.adsData),{},{adType:Un(i)?e.adsData.adType:i,adTagUrlTemplate:Un(r)?e.adsData.adTagUrlTemplate:r})})}(e,t.payload.initiateParams));case"[COMMON] set new ad tag url template":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adTagUrlTemplate:t.payload})});case"[MONETIZATION] change ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adStatus:t.payload,adErrorMessage:null})});case"[MONETIZATION] change ad tag":var n=t.payload,r=n.adUnit,i=n.adTag;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{currentAdTag:i,adUnit:r})});case"[MONETIZATION] change pending ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{pendingAdStatus:t.payload})});case"[MONETIZATION] change ad error":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adStatus:"error",adErrorMessage:t.payload})});case"[MONETIZATION] increase ad impression counter":var o=e.adsData.adImpression;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adImpression:o+1})});case"[MONETIZATION] increase ad Opportunity counter":var a=e.adsData.adOpportunity;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOpportunity:a+1})});case"[MONETIZATION] add played midroll number":var s=e.adsData.playedMidrolls,u=In()(s);return u.push(t.payload),Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOrder:t.payload,playedMidrolls:u})});case"[MONETIZATION] clear played midrolls":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{playedMidrolls:[]})});case"[MONETIZATION] clear ad data":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adOrder:0,currentAdTag:null,adDuration:0,adUnit:""})});case"[MONETIZATION] change ad duration":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adDuration:t.payload})});case"[MONETIZATION] update is vast ad":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{isVastAd:t.payload})});case"[MONETIZATION] change ad current time":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adCurrentTime:t.payload})});case"[MONETIZATION] update ad muted":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adMuted:t.payload})});case"[MONETIZATION] change ad volume":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{adVolume:t.payload})});case"[MONETIZATION] change pod info":var c=t.payload,l=c.podNumber,d=c.slotNumber;return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{podNumber:l,slotNumber:d})});case"[MONETIZATION] change loading ad status":return Sa(Sa({},e),{},{adsData:Sa(Sa({},e.adsData),{},{loadingAd:t.payload})});default:return e}},mediaData:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:ja,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Va({},function(e,t){var n=t.content_type,r=t.media_id,i=t.display_title;return Va(Va({},e),{},{mediaType:Un(n)?e.mediaType:n,mediaId:Un(r)?e.mediaId:r,videoData:Va(Va({},e.videoData),{},{showTitle:!!Un(i)||i})})}(e,t.payload.initiateParams));case"[CORE] load video request":return Va(Va({},e),{},{loadingMedia:!0});case"[CORE] load video request success":return Va(Va({},e),{},{loadingMedia:!1,videoList:t.payload});case"[CORE] set current video":var n=t.payload,r=n.index,i=n.videoData;return Va(Va({},e),{},{activeVideoIndex:r,videoData:i});case"[CORE] load video request error":return Va(Va({},e),{},{loadingMedia:!1,mediaLoadingError:t.payload});case"[COMMON] media request":var o=t.payload.mediaRequestObject;return Va(Va({},e),{},{mediaRequest:Va({},o)});default:return e}},semanticOptions:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Ba,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Fa({},function(e,t){var n=t.semantic_options;if(Un(n))return e;var r=n.minimum_date_factor,i=n.promoted_videos,o=n.scan_images_on_page,a=n.scanned_element,s=n.scanned_element_type,u=n.scoped_keywords,c=n.tags;return Fa(Fa({},e),{},{minimumDateFactor:Un(r)?e.minimumDateFactor:r,promotedVideos:Un(i)?e.promotedVideos:i,scanImagesOnPage:Un(o)?e.scanImagesOnPage:o,scannedElement:Un(a)?e.scannedElement:a,scannedElementType:Un(s)?e.scannedElementType:s,scopedKeywords:Un(u)?e.scopedKeywords:u,tags:Un(c)?e.tags:c})}(e,t.payload.initiateParams));default:return e}},userInteraction:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Wa,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[USER INTERACTION] change user interaction":return qa(qa({},e),{},{userInteractionType:t.payload});default:return e}},splitView:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:$a,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Ga({},function(e,t){var n=t.anchor_options;if(!Un(n)){var r=n.split_view,i=n.split_view_ratio;return Ga(Ga({},e),{},{splitViewRatio:Un(r)||!r||Un(i)?e.splitViewRatio:i})}return e}(e,t.payload.initiateParams));default:return e}},discovery:function(){var e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:Za,t=arguments.length>1?arguments[1]:void 0;switch(t.type){case"[CORE] initiate store":return Ya({},function(e,t){var n=t.next_video;return Un(n)?e:Ya(Ya({},e),{},{nextVideo:Xa(n)})}(e,t.payload.initiateParams));case"[DISCOVERY] show up next":return Ya(Ya({},e),{},{showUpNext:t.payload});case"[DISCOVERY] show skippable content":return Ya(Ya({},e),{},{showSkippableContent:t.payload});default:return e}}}),Qa=[],es=!1,ts=function e(){return function(t){return function(n){if(es)return Qa.push(n),null;es=!0;var r=t(n);return es=!1,Qa.length>0&&e()(t)(Qa.shift()),r}}},ns=function(e){var t=[];if(function(e){return!Un(e)&&!Un(e.enable_redux_debugging)&&e.enable_redux_debugging}(e)){var n=window&&window.__REDUX_DEVTOOLS_EXTENSION__&&window.__REDUX_DEVTOOLS_EXTENSION__();"function"===typeof n&&t.push(n)}var r=Et.apply(void 0,[wt(ua,ts)].concat(t));return vt(Ja,r)},rs=function(){function e(t){Ai()(this,e),f()(this,"playerVisibilitySubscriber",void 0),f()(this,"videoTagStatusSubscriber",void 0),f()(this,"shouldPlayIfLazyplay",!0),f()(this,"shouldPlayIfAutoplayWhenViewable",!0),f()(this,"videoPausedByObserver",!1),this.store=t,this.playerVisibilitySubscriber=null,this.videoTagStatusSubscriber=null,this.playAccordingToPlaybackMethod()}return Vi()(e,[{key:"lazyplayHandler",value:function(e){hn.playerVisibility(e)>=.5&&(this.playVideo(),this.shouldPlayIfLazyplay=!1)}},{key:"autoplayWhenViewableHandler",value:function(e){hn.playerVisibility(e)>=.5?this.playVideo():this.pauseVideo()}},{key:"onPlayerVisibilityChanged",value:function(e){var t=hn.playbackMethod(e);"lazyplay"===t&&this.shouldPlayIfLazyplay&&this.lazyplayHandler(e),"autoplay_when_viewable"===t&&this.shouldPlayIfAutoplayWhenViewable&&this.autoplayWhenViewableHandler(e)}},{key:"onVideoTagStatusChanged",value:function(e){var t=hn.videoTagStatus(e);"paused"!==t||this.videoPausedByObserver||(this.shouldPlayIfAutoplayWhenViewable=!1),"playing"===t&&(this.shouldPlayIfAutoplayWhenViewable=!0,this.videoPausedByObserver=!1)}},{key:"initiatePlayerVisibilitySubscriber",value:function(){this.playerVisibilitySubscriber=new ji(this.store,e.getPlayerVisibilityDependencies,this.onPlayerVisibilityChanged.bind(this))}},{key:"initiateVideoTagStatusSubscriber",value:function(){this.videoTagStatusSubscriber=new ji(this.store,e.getVideoTagStatusDependencies,this.onVideoTagStatusChanged.bind(this))}},{key:"playVideo",value:function(){var e=this.store,t=e.dispatch,n=e.getState;"idle"===hn.videoTagStatus(n())?on("play")(t):on("resume")(t)}},{key:"pauseVideo",value:function(){var e=this.store,t=e.dispatch,n=e.getState;"paused"!==hn.videoTagStatus(n())&&(this.videoPausedByObserver=!0,on("pause")(t))}},{key:"playAccordingToPlaybackMethod",value:function(){var e=this.store,t=e.dispatch,n=(0,e.getState)();switch(hn.playbackMethod(n)){case"autoplay":this.playVideo();break;case"lazyplay":this.initiatePlayerVisibilitySubscriber();break;case"autoplay_when_viewable":this.initiatePlayerVisibilitySubscriber(),this.initiateVideoTagStatusSubscriber();break;case"none":an(!1)(t)}}}],[{key:"getPlayerVisibilityDependencies",value:function(e){return[hn.playerVisibility(e)]}},{key:"getVideoTagStatusDependencies",value:function(e){return[hn.videoTagStatus(e)]}}]),e}(),is=function(){function e(t,n,r,i){var o=this;Ai()(this,e),f()(this,"videoStatusSubscriber",void 0),f()(this,"videoListSubscriber",void 0),f()(this,"mediaRequestSubscriber",void 0),f()(this,"playerVisibilitySubscriber",void 0),f()(this,"playbackMethodManager",void 0),f()(this,"store",void 0),f()(this,"loadContent",function(e,t,n,r){o.loadMedia(t,n,r).then(function(){o.playbackMethodManager=new rs(e)})}),f()(this,"loadMedia",function(e,t,n){var r=o.store,i=r.dispatch,a=r.getState,s=Dn.showTitle(a());if("semantic"===e){var u=pn.semanticOptions(a());return Na(u,s,n)(i)}return ka(t,s,n)(i)}),this.store=t,this.videoStatusSubscriber=new ji(t,e.getVideoStatusDependencies,this.onVideoStatusChanged.bind(this)),this.videoListSubscriber=new ji(t,e.getVideoListDependencies,this.onVideoListChanged.bind(this)),this.mediaRequestSubscriber=new ji(t,e.getMediaRequestDependencies,this.onMediaRequestChanged.bind(this)),this.playerVisibilitySubscriber=null,this.loadContent(t,r,n,i)}return Vi()(e,null,[{key:"createInstance",value:function(t,n,r,i){return new e(t,n,r,i)}}]),Vi()(e,[{key:"playNextVideo",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e),r=Cn.activeVideoIndex(e)+1;n.length>1&&r>=n.length&&(r=0),r<n.length&&(!function(e){e({type:"[CORE] reset player data time params"})}(t),La(r,n[r])(t),on("play")(t))}},{key:"playPreviousVideo",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e),r=Cn.activeVideoIndex(e);if(r>0){var i=r-1;La(i,n[i])(t),on("play")(t)}}},{key:"onVideoStatusChanged",value:function(e){"complete"===hn.videoTagStatus(e)&&this.playNextVideo(e)}},{key:"onVideoListChanged",value:function(e){var t=this.store.dispatch,n=Cn.videoList(e);!jn(n)&&n.length>0&&La(0,n[0])(t)}},{key:"onMediaRequestChanged",value:function(e){var t=Cn.mediaRequest(e);switch(t.type){case"playNewVideo":this.loadMedia("specific",t.value);break;case"playNextVideo":this.playNextVideo(e);break;case"playPreviousVideo":this.playPreviousVideo(e)}}}],[{key:"getVideoStatusDependencies",value:function(e){return[hn.videoTagStatus(e)]}},{key:"getVideoListDependencies",value:function(e){return[Cn.videoList(e)]}},{key:"getMediaRequestDependencies",value:function(e){return[Cn.mediaRequest(e)]}}]),e}(),os=function e(t){var n=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"onDependencyFailure",function(e,t){console.log("onDependencyFailure",e,t);var r=n.store,i=r.dispatch,o=r.getState;switch(e){case"ima":"blocked"!==Fi.loadingImaStatus(o())&&Qn("error")(i);break;case"hls":er("error")(i)}}),f()(this,"onDependencyReady",function(e){var t=n.store.dispatch;switch(e){case"ima":Qn("success")(t);break;case"hls":er("success")(t)}}),this.store=t},as=function(e){return function(t){t({type:"[COMMON] set fullscreen",payload:e})}},ss=function(){function e(t,n){var r=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"videoTag",void 0),f()(this,"pendingFullscreenSubscriber",void 0),f()(this,"adStatusSubscriber",void 0),f()(this,"playerUniqId",void 0),f()(this,"onAdStatusChanged",function(e){var t=_i.adStatus(e),n=r.videoTag.webkitDisplayingFullscreen;"playing"===t&&Bn()&&n&&r.exitFullscreen(r.videoTag)}),f()(this,"isPlayerInFullscreen",function(){var e=document,t=Bn()?En(r.playerUniqId):bn(r.playerUniqId);return Un(e.fullscreenElement)?!Un(e.webkitFullscreenElement)&&0===e.webkitFullscreenElement.id.localeCompare(t):0===e.fullscreenElement.id.localeCompare(t)}),f()(this,"changePlayerWidth",function(e){r.videoTag.style.width=e?"100%":"auto"}),f()(this,"onFullscreenChanged",function(){var e=r.store.dispatch,t=r.isPlayerInFullscreen();r.changePlayerWidth(t),as(t)(e)}),f()(this,"onFullscreenChangedIos",function(){var e=r.store.dispatch,t=r.videoTag.webkitDisplayingFullscreen;t||on("resume")(e),r.changePlayerWidth(t),as(t)(e)}),f()(this,"onPendingFullscreenRequestChanged",function(e){var t=gn.pendingFullscreenRequest(e);"enter"===t?r.enterFullscreen(r.videoTag):"exit"===t&&r.exitFullscreen(r.videoTag)}),f()(this,"getFullScreenElement",function(e,t){var n=document.getElementById(bn(r.playerUniqId));return Bn()?t:e?document:n}),f()(this,"enterFullscreen",function(e){var t=r.getFullScreenElement(!1,e);Bn()?t.webkitEnterFullscreen():document.webkitExitFullscreen?t.webkitRequestFullscreen():document.webkitCancelFullScreen?t.webkitRequestFullScreen():document.mozCancelFullScreen?t.mozRequestFullScreen():document.msExitFullscreen&&t.msRequestFullscreen()}),f()(this,"exitFullscreen",function(e){var t=r.getFullScreenElement(!0,e);document.webkitExitFullscreen||Bn()?t.webkitExitFullscreen():document.webkitCancelFullScreen?t.webkitCancelFullScreen():document.mozCancelFullScreen?t.mozCancelFullScreen():document.msExitFullscreen&&t.msExitFullscreen()}),this.store=t,this.videoTag=document.getElementById(En(n)),this.playerUniqId=n,document.addEventListener("fullscreenchange",this.onFullscreenChanged.bind(this)),document.addEventListener("webkitfullscreenchange",this.onFullscreenChanged.bind(this)),Bn()&&(this.videoTag.addEventListener("webkitendfullscreen",this.onFullscreenChangedIos.bind(this)),this.videoTag.addEventListener("webkitbeginfullscreen",this.onFullscreenChangedIos.bind(this))),this.pendingFullscreenSubscriber=new ji(t,e.getPendingFullscreenDependencies,this.onPendingFullscreenRequestChanged.bind(this)),this.adStatusSubscriber=new ji(t,e.getAdStatusDependencies,this.onAdStatusChanged.bind(this))}return Vi()(e,null,[{key:"createInstance",value:function(t,n){return new e(t,n)}}]),Vi()(e,null,[{key:"getPendingFullscreenDependencies",value:function(e){return[gn.pendingFullscreenRequest(e)]}},{key:"getAdStatusDependencies",value:function(e){return[_i.adStatus(e)]}}]),e}();function us(e,t){var n=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter(function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable})),n.push.apply(n,r)}return n}function cs(e){for(var t=1;t<arguments.length;t++){var n=null!=arguments[t]?arguments[t]:{};t%2?us(Object(n),!0).forEach(function(t){f()(e,t,n[t])}):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(n)):us(Object(n)).forEach(function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(n,t))})}return e}var ls,ds=function(e){return function(e){return e&&window.monti.playerConfigs&&window.monti.playerConfigs[e]}(e)?function(e){return window.monti.playerConfigs[e]}(e):window.monti.playerConfigs?window.monti.playerConfigs&&window.monti.playerConfigs[Object.keys(window.monti.playerConfigs)[0]]:null},ps=function e(t){var n=this;Ai()(this,e),f()(this,"videoTag",void 0),f()(this,"isBufferError",void 0),f()(this,"hls",void 0),f()(this,"hlsSetup",function(e,t,r,i){n.initiateHls(e),n.loadHlsSource(e,t,r,i)}),f()(this,"detachMedia",function(){Un(n.hls)||(n.hls.detachMedia(),n.hls.destroy(),n.hls=null)}),f()(this,"initiateHls",function(e){n.hls=new e,n.hls.attachMedia(n.videoTag)}),f()(this,"loadHlsSource",function(e,t,r,i){n.hls.on(e.Events.MEDIA_ATTACHED,function(){n.hls.loadSource(t)}),n.hls.on(e.Events.ERROR,function(t,o){n.mapHlsToErrors(e,o,i),t.details===e.ErrorDetails.BUFFER_STALLED_ERROR&&(r(!0),n.isBufferError=!0)}),n.hls.on(e.Events.FRAG_BUFFERED,function(){n.isBufferError&&(r(!1),n.isBufferError=!1)})}),f()(this,"mapHlsToErrors",function(e,t,r){if(t.fatal)switch(t.type){case 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t=hn.pendingVideoTagStatus(e),n=Dn.sources(e),i=Fi.loadingHLSStatus(e),o="blocked"===Fi.loadingImaStatus(e);r.handlePendingVideoStatus(t,n,i,o)}),f()(this,"onVideoDataChanged",function(){r.newVideoDataLoaded=!0}),f()(this,"sendPrerollPlayRequest",function(){var e=r.store.dispatch;hs("playPreroll")(e)}),f()(this,"handlePlayRequest",function(e,t,n){var i=r.store.dispatch;if(e&&e.length>0){if(r.newVideoDataLoaded&&(r.loadVideoSource(r.videoTag,e,t),r.newVideoDataLoaded=!1,r.prerollEnabled&&!n))return void r.sendPrerollPlayRequest();r.videoTag.play().catch(function(e){return console.error("Error playing the video: ",e)})}else dn(Xn.VIDEO_ERROR)(i)}),f()(this,"handlePendingVideoStatus",function(e,t,n,i){switch(e.type){case"play":r.handlePlayRequest(t,n,i);break;case"resume":r.videoTag.play().catch(function(e){return console.error("Error resuming the video: ",e)});break;case"pause":r.videoTag.pause();break;case"replay":r.videoTag.currentTime=0,r.videoTag.play().catch(function(e){return console.error("Error replaying the video: ",e)});break;case"seekTo":r.videoTag.pause(),r.videoTag.currentTime=e.value}}),f()(this,"loadMp4Source",function(e,t,n){var r=Ra(t,ys);n.setAttribute("src",r),n.load()}),f()(this,"loadVideoSource",function(e,t,n){var i=r.store.dispatch,o=Ra(t,gs);switch(fs.suitableVideoSource(e,o,n)){case"mp4":r.loadMp4Source(n,t,e);break;case"m3u8 with hls":r.videoStreamingManager.hlsLibrarySetup(e,o,function(e){return un(e)(i)},function(e){return dn(e)(i)});break;case"m3u8 directly":fs.loadHlsVideoDirectly(e,o)}}),this.store=t;var i=t.getState;this.videoStreamingManager=new fs,this.videoTag=document.getElementById(En(n)),this.prerollEnabled=bi.prerollEnabled(i()),this.pendingVideoStatusSubscriber=new ji(t,e.getPendingVideoStatusDependencies,this.onPendingVideoStatusChanged.bind(this)),this.videoDataSubscriber=new ji(t,e.getVideoDataDependencies,this.onVideoDataChanged.bind(this)),this.hlsLoadingStatusSubscriber=new ji(t,e.getHLSLoadingStatusDependencies,this.onHlsLoadingStatusChanged.bind(this))}return Vi()(e,null,[{key:"createInstance",value:function(t,n){return new e(t,n)}}]),Vi()(e,null,[{key:"getHLSLoadingStatusDependencies",value:function(e){return[Fi.loadingHLSStatus(e)]}},{key:"getPendingVideoStatusDependencies",value:function(e){return[hn.pendingVideoTagStatus(e)]}},{key:"getVideoDataDependencies",value:function(e){return[Cn.videoData(e)]}}]),e}();function ms(e,t){var n=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter(function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable})),n.push.apply(n,r)}return n}function bs(e){for(var t=1;t<arguments.length;t++){var n=null!=arguments[t]?arguments[t]:{};t%2?ms(Object(n),!0).forEach(function(t){f()(e,t,n[t])}):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(n)):ms(Object(n)).forEach(function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(n,t))})}return e}var Os={READY_EVENT:"ready",PLAY_EVENT:"play",PAUSE_EVENT:"pause",TIME_EVENT:"time",SEEK_EVENT:"seek",COMPLETE_EVENT:"complete",VOLUME_EVENT:"volume",MUTE_EVENT:"mute"},_s=Object.values(Os),Ss={FULLSCREEN_EVENT:"fullscreen",ANCHOR_STATUS_EVENT:"anchorStatusChanged",ANCHOR_CLOSED_EVENT:"anchorClosed"},Es={AD_PLAY_EVENT:"adPlay",AD_PAUSE_EVENT:"adPause",AD_RESUME_EVENT:"adResume",AD_COMPLETE_EVENT:"adComplete",AD_TIME_EVENT:"adTime",AD_MUTE_EVENT:"adMute",AD_SKIPPED_EVENT:"adSkipped",AD_ERROR_EVENT:"adError",AD_BLOCK_EVENT:"adBlock",AD_REQUEST_EVENT:"adRequest",AD_OPPORTUNITY_EVENT:"adOpportunity",AD_IMPRESSION_EVENT:"adImpression"},ws=Object.values(Es),Ps=Object.values(bs(bs(bs({},Os),Es),Ss)),Ts=function(){function e(t,n){var r=this;Ai()(this,e),f()(this,"eventsCallbacksHandler",void 0),f()(this,"store",void 0),f()(this,"videoStatusSubscriber",void 0),f()(this,"videoMuteSubscriber",void 0),f()(this,"videoVolumeSubscriber",void 0),f()(this,"videoTimeFragmentSubscriber",void 0),f()(this,"videoListStoreSubscriber",void 0),f()(this,"previousVideoTagStatus",void 0),f()(this,"startSeekTime",0),f()(this,"canHandleReady",function(e,t,n){if(t===Os.READY_EVENT){var r=Cn.videoList(e);if(Array.isArray(r)&&r.length>0)return n(),!0}return!1}),f()(this,"canBeHandled",function(e,t){var n=r.store.getState;return r.canHandleReady(n(),e,t)}),f()(this,"reportSeekEnd",function(e){var t={position:hn.currentVideoTimeFragment(e),offset:r.startSeekTime};r.eventsCallbacksHandler.onEvent(Os.SEEK_EVENT,t)}),f()(this,"onMuteStateChanged",function(e){var t=gn.muted(e);r.eventsCallbacksHandler.onEvent(Os.MUTE_EVENT,{state:t})}),f()(this,"onVolumeChanged",function(e){var t=gn.muted(e),n=gn.volume(e);r.eventsCallbacksHandler.onEvent(Os.VOLUME_EVENT,{level:t?0:n})}),f()(this,"onVideoTimeFragmentChanged",function(e){var t=hn.currentVideoTimeFragment(e),n=hn.currentVideoDuration(e);r.eventsCallbacksHandler.onEvent(Os.TIME_EVENT,{duration:n,position:t})}),f()(this,"onVideoListChanged",function(){r.eventsCallbacksHandler.onEvent(Os.READY_EVENT)}),this.store=t,this.eventsCallbacksHandler=n,this.videoStatusSubscriber=new ji(t,e.getVideoStatusDependencies,this.onVideoStatusChanged.bind(this)),this.videoMuteSubscriber=new ji(t,e.getVideoMuteDependencies,this.onMuteStateChanged.bind(this)),this.videoVolumeSubscriber=new ji(t,e.getVolumeDependencies,this.onVolumeChanged.bind(this)),this.videoTimeFragmentSubscriber=new ji(t,e.getVideoTimeDependencies,this.onVideoTimeFragmentChanged.bind(this)),this.videoListStoreSubscriber=new ji(t,e.getVideoListDependencies,this.onVideoListChanged.bind(this)),this.previousVideoTagStatus=hn.videoTagStatus(t.getState())}return Vi()(e,[{key:"onVideoStatusChanged",value:function(e){var t=hn.videoTagStatus(e);switch("seeking"===this.previousVideoTagStatus&&this.reportSeekEnd(e),t){case"paused":this.eventsCallbacksHandler.onEvent(Os.PAUSE_EVENT);break;case"seeking":this.startSeekTime=hn.currentVideoTimeFragment(e);break;case"complete":this.eventsCallbacksHandler.onEvent(Os.COMPLETE_EVENT);break;case"playing":this.eventsCallbacksHandler.onEvent(Os.PLAY_EVENT)}this.previousVideoTagStatus=t}}],[{key:"getVideoStatusDependencies",value:function(e){return[hn.videoTagStatus(e)]}}]),e}();f()(Ts,"getVideoMuteDependencies",function(e){return[gn.muted(e)]}),f()(Ts,"getVolumeDependencies",function(e){return[gn.volume(e)]}),f()(Ts,"getVideoTimeDependencies",function(e){return[hn.currentVideoTimeFragment(e)]}),f()(Ts,"getVideoListDependencies",function(e){return[Cn.videoList(e)]}),f()(Ts,"isContentEvent",function(e){return _s.some(function(t){return t===e})});var As=function e(t,n){var r=this;Ai()(this,e),f()(this,"eventsCallbacksHandler",void 0),f()(this,"store",void 0),f()(this,"fullscreenSubscriber",void 0),f()(this,"anchorStatusSubscriber",void 0),f()(this,"anchorDisabledByUserSubscriber",void 0),f()(this,"onFullscreenChanged",function(e){var t=gn.isFullscreenOn(e);r.eventsCallbacksHandler.onEvent(Ss.FULLSCREEN_EVENT,{state:t})}),f()(this,"onAnchorStatusChanged",function(e){var t="active"===Pr(e)?"activated":"deactivated";r.eventsCallbacksHandler.onEvent(Ss.ANCHOR_STATUS_EVENT,{state:t})}),f()(this,"onAnchorDisabledByUser",function(e){if(wr(e)){var t=hn.currentVideoTimeFragment(e);r.eventsCallbacksHandler.onEvent(Ss.ANCHOR_CLOSED_EVENT,{position:t})}}),this.store=t,this.eventsCallbacksHandler=n,this.fullscreenSubscriber=new ji(t,e.getFullscreenDependencies,this.onFullscreenChanged.bind(this)),this.anchorStatusSubscriber=new ji(t,e.getAnchorStatusDependencies,this.onAnchorStatusChanged.bind(this)),this.anchorDisabledByUserSubscriber=new ji(t,e.getAnchorDisabledByUserDependencies,this.onAnchorDisabledByUser.bind(this))};f()(As,"getFullscreenDependencies",function(e){return[gn.isFullscreenOn(e)]}),f()(As,"getAnchorStatusDependencies",function(e){return[Pr(e)]}),f()(As,"getAnchorDisabledByUserDependencies",function(e){return[wr(e)]});var Cs=function(){function e(t,n){var r=this;Ai()(this,e),f()(this,"store",void 0),f()(this,"eventsCallbacksHandler",void 0),f()(this,"adStatusSubscriber",void 0),f()(this,"adImpressionSubscriber",void 0),f()(this,"adOpportunitySubscriber",void 0),f()(this,"adTimeSubscriber",void 0),f()(this,"adMuteSubscriber",void 0),f()(this,"adProviderLoadingStatusSubscriber",void 0),f()(this,"previousAdStatus",void 0),f()(this,"canBeHandled",function(e,t){var n=r.store.getState;switch(Fi.loadingImaStatus(n())){case"loading":return!1;case"success":case"error":return!0;case"blocked":return t(),!0;case"":default:return!1}}),f()(this,"onAdStatusChanged",function(e){var t=_i.adStatus(e),n=_i.currentAdTag(e);switch(t){case"requested":r.eventsCallbacksHandler.onEvent(Es.AD_REQUEST_EVENT,{tag:n});break;case"paused":r.eventsCallbacksHandler.onEvent(Es.AD_PAUSE_EVENT,{tag:n});break;case"completed":r.eventsCallbacksHandler.onEvent(Es.AD_COMPLETE_EVENT,{tag:n});break;case"skipped":r.eventsCallbacksHandler.onEvent(Es.AD_SKIPPED_EVENT,{tag:n});break;case"playing":"paused"===r.previousAdStatus?r.eventsCallbacksHandler.onEvent(Es.AD_RESUME_EVENT,{tag:n}):r.eventsCallbacksHandler.onEvent(Es.AD_PLAY_EVENT,{tag:n});break;case"error":var i=_i.adErrorMessage(e);r.eventsCallbacksHandler.onEvent(Es.AD_ERROR_EVENT,{tag:n,message:i})}r.previousAdStatus=t}),f()(this,"onAtTimeChanged",function(e){var t=_i.adCurrentTime(e),n=_i.currentAdTag(e),i=_i.adDuration(e);r.eventsCallbacksHandler.onEvent(Es.AD_TIME_EVENT,{position:t,tag:n,duration:i})}),f()(this,"onAdMuteChanged",function(e){var t=_i.adMuted(e);r.eventsCallbacksHandler.onEvent(Es.AD_MUTE_EVENT,{state:t})}),f()(this,"onAdProviderLoadingChanged",function(e){"blocked"===Fi.loadingImaStatus(e)&&r.eventsCallbacksHandler.onEvent(Es.AD_BLOCK_EVENT)}),f()(this,"onAdImpressionChanged",function(e){var t=_i.currentAdTag(e);r.eventsCallbacksHandler.onEvent(Es.AD_IMPRESSION_EVENT,{tag:t})}),f()(this,"onAdOpportunityChanged",function(e){var t=_i.currentAdTag(e);r.eventsCallbacksHandler.onEvent(Es.AD_OPPORTUNITY_EVENT,{tag:t})}),this.store=t,this.eventsCallbacksHandler=n,this.previousAdStatus=_i.adStatus(t.getState()),this.adStatusSubscriber=new ji(t,e.getAdStatusDependencies,this.onAdStatusChanged.bind(this)),this.adTimeSubscriber=new ji(t,e.getAdTimeDependencies,this.onAtTimeChanged.bind(this)),this.adMuteSubscriber=new ji(t,e.getAdMuteDependencies,this.onAdMuteChanged.bind(this)),this.adImpressionSubscriber=new ji(t,e.getAdImpressionDependencies,this.onAdImpressionChanged.bind(this)),this.adOpportunitySubscriber=new ji(t,e.getAdOpportunitySubscriberDependencies,this.onAdOpportunityChanged.bind(this)),this.adProviderLoadingStatusSubscriber=new ji(t,e.getAdProviderLoadingDependencies,this.onAdProviderLoadingChanged.bind(this))}return Vi()(e,null,[{key:"getAdStatusDependencies",value:function(e){return[_i.adStatus(e)]}},{key:"getAdTimeDependencies",value:function(e){return[_i.adCurrentTime(e)]}},{key:"getAdMuteDependencies",value:function(e){return[_i.adMuted(e)]}},{key:"getAdProviderLoadingDependencies",value:function(e){return[Fi.loadingImaStatus(e)]}}]),e}();f()(Cs,"isAdEvent",function(e){return ws.some(function(t){return t===e})}),f()(Cs,"getAdImpressionDependencies",function(e){return[_i.adImpression(e)]}),f()(Cs,"getAdOpportunitySubscriberDependencies",function(e){return[_i.adOpportunity(e)]});var Rs=function e(t){var n=this;Ai()(this,e),f()(this,"contentEvents",void 0),f()(this,"generalEvents",void 0),f()(this,"adEvents",void 0),f()(this,"subscribers",{}),f()(this,"onRegisterToEvent",function(e,t,r){n.isValidEvent(e)&&(Ts.isContentEvent(e)&&n.contentEvents.canBeHandled(e,t)||Cs.isAdEvent(e)&&n.adEvents.canBeHandled(e,t())||n.getEventSubscribersList(e).push({callback:t,once:r}))}),f()(this,"isValidEvent",function(e){return Ps.some(function(t){return t===e})}),f()(this,"getEventSubscribersList",function(e){return Array.isArray(n.subscribers[e])||(n.subscribers[e]=[]),n.subscribers[e]}),f()(this,"filterOutOnceCallbacks",function(e,t){n.subscribers[e]=t.filter(function(e){return!e.once})}),f()(this,"onEvent",function(e,t){var r=n.getEventSubscribersList(e);r.forEach(function(e){(0,e.callback)(t)}),n.filterOutOnceCallbacks(e,r)}),this.contentEvents=new Ts(t,this),this.generalEvents=new As(t,this),this.adEvents=new Cs(t,this)},Ds=function(){function e(t){Ai()(this,e),f()(this,"eventsHandler",void 0),this.eventsHandler=new Rs(t)}return Vi()(e,[{key:"on",value:function(e,t){this.eventsHandler.onRegisterToEvent(e,t,!1)}},{key:"once",value:function(e,t){this.eventsHandler.onRegisterToEvent(e,t,!0)}}]),e}();function Ms(e,t){var n=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter(function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable})),n.push.apply(n,r)}return n}function Is(e){for(var t=1;t<arguments.length;t++){var n=null!=arguments[t]?arguments[t]:{};t%2?Ms(Object(n),!0).forEach(function(t){f()(e,t,n[t])}):Object.getOwnPropertyDescriptors?Object.defineProperties(e,Object.getOwnPropertyDescriptors(n)):Ms(Object(n)).forEach(function(t){Object.defineProperty(e,t,Object.getOwnPropertyDescriptor(n,t))})}return e}var ks=function(){var e=window.monti.dataset;return jn(e)?(e={players:{},preact:Is(Is({},r),i),store:{},plugins:{}},window.monti.dataset=e,e):e},Ns=function(e){var t=function(){var e=(new Date).getTime(),t=performance&&performance.now&&1e3*performance.now()||0;return"xxxxxxxx-xxxx-4xxx-yxxx-xxxxxxxxxxxx".replace(/[xy]/g,function(n){var r=16*Math.random();return e>0?(r=(e+r)%16|0,e=Math.floor(e/16)):(r=(t+r)%16|0,t=Math.floor(t/16)),("x"===n?r:3&r|8).toString(16)})}(),n=function(e,t){var n=ns(e.dev_config),r=ks(),i=n.dispatch;return r.store[t]=n,function(e,t){return function(n){n({type:"[CORE] initiate store",payload:{initiateParams:e,playerInstanceUniqId:t}})}}(e,t)(i),n}(e,t);return function(e,t,n){B(b(Pi,{playerId:t,store:n,playerPosition:e}),e)}(e.player_pos,t,n),oa.getInstance().loadInternalPlugins(n,t,e),ss.createInstance(n,t),vs.createInstance(n,t),is.createInstance(n,e.media_id,e.content_type,e.dev_config),function(e){var t=e.dispatch;if(er(Ci.getInstance().getHLSLoadingStatus())(t),Qn(Ci.getInstance().getIMALoadingStatus())(t),!Ci.getInstance().isDependenciesReady()){var n=new os(e);Ci.getInstance().addDependenciesCallback(n)}}(n),function(e,t){ks().players[t]=new Ds(e)}(n,t),t},Ls=function(e){return console.log("player initiation start",e),new Promise(function(t,n){try{var r=function(e){var t=e.player_pos||document.currentScript.parentElement,n=e.media_id||e.content_id;return cs(cs({},e),{},{player_pos:t,media_id:n})}(function(e){var t=e.player_id,n=ds(t);return null===n?e:cs(cs({},e),n)}(e)),i=Ns(r);!function(e){var t=new CustomEvent("montiConfigLoaded",{detail:{playerKey:e}});window.dispatchEvent(t)}(i),t(i)}catch(o){console.error("Player initiation error",o),n(o)}})},xs=function(){return{initiate:Ls}};window.monti=xs,Ci.getInstance().loadExternalDependencies()}]); window.monti().initiate(Object.assign({player_pos: document.currentScript.parentElement}, {"is_conflicting_with_other_jw_players":false,"programmatic_play_with_sound_on_desktop":false,"referrer_id":"af93e181-b289-0560-a2bf-808e93bb05bc","width":"100","comscore_publisher_id":"18120612","monetization":{"ad_type":"static_tag","continue_content_play_while_waiting_for_ad":false,"strategy":"on_player_load","ad_request_timeout":"10000","midrolls":{"on":[0]},"vpaid_mode":"ENABLED","ad_tag":"https://pubads.g.doubleclick.net/gampad/ads?sz=400x300|640x480|480x270|640x360&iu=/175840252/MMPlus/smithsonianmag/Video&impl=s&gdfp_req=1&env=vp&output=vast&unviewed_position_start=1&url=##REFERRER_URL_UNESC##&description_url=##DESCRIPTION_URL_UNESC##&correlator=##CACHEBUSTER##&cust_params=mm_midroll%3D##MIDROLL_ORDER##%26video_ID%3D##VIDEO_ID##"},"sponsorship":false,"player_identifier":"mplayer","recommendation_id":null,"brand_color":"#FF9900","powered_by_strip":true,"platform":"buffy","type":"video","config_name":"MM+ | Smithsonianmag | Podding","player_id":"3v9g2u2f","playlist_id":"fSkmeWKF","playback_method":"autoplay","anchor_viewability_method":"none","player_version":"v4","playlist_type":"semantic","semantic_options":{"scan_images_on_page":true,"scanned_element":"","tags":"geogrophy,nature,animals,habitat,outdoors,science,history","minimum_date_factor":30,"scanned_element_type":"tag","scoped_keywords":"mentalfloss","promoted_videos":[]},"script_destination":"mm","publisher_contribution":"floor8","general_script_description":"","brand_logo":"","brand_logo_click_url":"","next_video":"none","uniq_key":"af93e181-b289-0560-a2bf-808e93bb05bc","content_id":"fSkmeWKF","content_type":"semantic"})); Finland has vastly improved in reading, math and science literacy over the past decade in large part because its teachers are trusted to do whatever it takes to turn young lives around. This 13-year-old, Besart Kabashi, received something akin to royal tutoring.

      Kari Louhiuori, a principal at a Finnish school made a mostly unheaard of and uncanny decision to hold back an immigrant student from 6th grader named Besart because he hadnʻt felt that this young man was falling behind due to laziness but to a lack of comprehension. After a year of "royal tutoring," by allowing the boy to read at his own pace, it worked!

    1. Reviewer #3

      The work by Barros et al. looks at the role of the Ribosome Quality Control pathway (RQC) in regulating the expression of endogenous messages containing polybasic sequences. Using ribosome profiling and western blotting, the authors show that proteins containing various types of polybasic sequences are not targeted by the RQC. The authors argue that one of the few endogenous RQC substrate, RQC1, is not regulated via the canonical RQC pathway, but by a Ltn1p-dependent post transcriptional mechanism.

      The question of whether there are endogenous RQC substrates has previously been explored. With the exception of the few identified substrates, such as RQC1 (Brandman et al, 2012) and SDD1 (Matsuo et al., 2020), these studies largely concluded the RQC has a minimal regulatory role for endogenous messages, and is most likely protecting cells from damage and environmental stressors. This idea is further supported by the observation that the RQC is non-essential under standard growth condition, but becomes synthetic lethal with translation inhibitors (Kostova et al, 2017, Choe et al, 2016). The work by Barros et al. comes to the same conclusions, and therefore it is unclear how this work contributes to the already established role of the RQC.

      The authors also explore the regulation of RQC1 by the RQC and argue that this gene is regulated by Ltn1p in an RQC-independent way. However, mechanistic understanding of the proposed regulation is lacking, and the data are largely inconsistent with the previously published observations by Brandman et al, 2012.

      Major points:

      1) The authors use the dataset published by Pop et al., 2014 for their 27-29 nt no drug ribosome profiling analysis. However, these no-drug samples have been reported to exhibit surprising heterogeneity, and similarities with CHX-pretreated samples (see Hussmann et al., 2015 for detailed analysis). It is unclear how this heterogeneity can affect the analysis in the current manuscript, and whether the authors were aware of these caveats. Have the authors used independent datasets to confirm their observations? Have they excluded replicas that show CHX-like characteristics, such as A-site occupancy bias similar to CHX pretreated samples?

      2) It is not clear what the purpose of the analysis presented in Fig 2 is, and how it is different from the modeling in the Park and Subramaniam 2019 paper? Are the authors using these parameters (TE, Kozak score, etc.) to show adaptations that minimize ribosome collisions?

      3) Fig 3 - some of the selected examples (Dbp3, Yro2, Nop58) lack sufficient coverage in the region of interested highlighted in the right column for the short and/or long footprints. Since the data are insufficient to make conclusions about ribosome stalling and queuing, these examples should be excluded from the analysis.

      4) Fig 4:

      -Does ASC1 deletion cause frameshifting? Since the TAP-tag is C-terminal, it is possible that it is now out of frame, and therefore undetectable. Is it possible for the authors to introduce the tag on the N-terminus, and follow simultaneously the stalled nascent polypeptide (upon LTN1 deletion), and the full length protein?

      -Is the putative stalling site of Dbp3 too close to the stat codon to cause collisions?

      -Can the authors include a positive control, such as TAP-tagged Sdd1 to make sure their assay works and their strains and KOs behave as expected?

      5) Fig 5:

      -What is causing the inconsistency with the Brandman et al., 2012 data about RQC-dependent regulation of RQC1? In the original paper, Rqc1p has an N-terminal FLAG tag, so the authors primarily follow the stalled nascent polypeptide, whereas the current study focuses on the full length protein. Can the authors compare the same construct (FLAG-tagged Rqc1p) in their strains, so it is an "apples to apples" comparison?

      -Fig 5c bottom panel - the read coverage is too sparse to make a conclusion. This analysis should be removed.

      -5 d, e. The comparison between the GFP-12R-RFP stalling reporter and RQC2-TAP is not fair. The GFP construct reports on the fate of the stalled nascent polypeptide, whereas the RQC1-TAP looks at the full-length protein, and remains blind to the putative stalling product. Can the authors change the location of the tag, and repeat the experiment now looking at the stalled nascent polypeptide for RQC1? In addition, the signal in Fig. 5e look saturated. Is it possible that no effect is observed simply because the TAP signal is out of the dynamic range for the assay?

      Minor Comments:

      1) The introduction presents an overly simplistic view of ribosome stalling, arguing that stalling can be caused by polybasic stretches. We now know that stalling is much more complex, and there are many other factors, including the presence of non-optimal codon pairs, that cause ribosome collisions. Although the authors discuss these factors in their discussion, they should also be emphasized in the introductory paragraph.

    1. At

      Sentence structure. Most everything carries that long sentence structure. At least in the beginning parts. If it changes I will tag in the bottom when I get there!

  5. Sep 2020
    1. The FBI said it has stopped using the "Black Identity Extremist" tag and acknowledged that white supremacist violence is the biggest terrorist threat this country faces.

      When using the "Always Check" Approach, this headline generated many relevant Google searches, with multiple other media outlets covering this. Hence, The Root appears to be credible. I'm very surprised that it took a long time for the FBI to make this decision.

    1. The <output> tag represents the result of a calculation. Typically this element defines a region that will be used to display text output from some calculation.

      How <output> tag can be used in HTML5

    1. D&D is the superior mental health resource

      Useful tag with stories and experiences

    1. globals are assumed to have their field value on the window object and can be referenced inside the bundle by their field name globals: { name: 'Value', }, assumes that some other script tag or whatever establishes window.Value and the emitted umd bundle for example, calls the factory like factory(global.Value). So globals is just stuff to bring into the factory on the globals object. It doesn't even make it "global" inside the bundle. Basically, the resolver does not check the globals object during the loading process. The resolver needs to be told how to link these globals and that's what the external option is for. external: ['name'], Then you can reference it like import myName from 'name'; myName();
    1. Reviewer #1:

      Previous work has shown that the nuclear import of TyrRS is stimulated under stress and that nucleus-localized TyrRS functions through the transcriptional machinery to promote the expression of DNA damage response genes for cell protection. In this work, evidence is presented that nuclear TyrRS also inhibits bulk translation in a manner correlated with its association with several AARS-encoding genes and that for elongation factor eEF1A, and recruitment to these genes of HDACs. Mutation of the TyrRS NLS, whose function in nuclear localization provides for coupling between low tRNATyr binding and nuclear localization, was found to derepress bulk translation after prolonged oxidative stress by H2O2, without altering eIF2 phosphorylation levels or mTOR activation, and overexpression (o/e) of TyrRS can reduce protein synthesis, in a manner enhanced by the E196K mutation associated with Charcot-Marie-Tooth disease (CMT), shown previously to enhance TyrRS association with transcriptional co-repressors. ChIP-Seq of overexpressed V5-tagged TyrRS showed binding to only 17 sites, of which 15 are within gene coding sequences, among which four encode TyrRS, TrpRS, SerRS and GlyRS, and a fifth encodes elongation factor eEF1A. These results were confirmed by ChIP analysis of endogenous TyrRS, using the HisRS gene as negative control; and the occupancies were shown to increase on H2O2 treatment. The expression of these AARS/eEF1A gene transcripts was shown to be reduced by o/e of TyrRS, in a manner enhanced for at least some of them by the E196K CMT mutation; and the repression was shown to be eliminated by the NLS_mut for YARS expressed at native levels. Reductions in AARS/eEF1A protein expression were also observed on WT TyrRS o/e. Sequence analysis of the genes showing TyrRS binding by ChIP-seq led to identification of a motif that was shown to be required for binding to TyrRS in vitro in EMSA assays with either purified TyrRS or in extracts from cells overexpressing it, in a manner requiring the full-length TyrRS and not only the catalytic core of the enzyme. It was not shown however that eliminating this motif from any of the target genes attenuated their repression by nuclear-localized TyrRS. Mass spec analysis of affinity-purified, overexpressed TyrRS identified interacting proteins, and several of which were shown to be coimmunoprecipitated with endogenous TyrRS in non-stressed cells, including the transcription cofactors Trim28, HDAC1, and subunits of the NURD co-repressor/histone deacetylase complex. ChIP assays showed that overexpression of TyrRS lead to decreased levels of H3K27Ac, a histone mark of active transcription, and elevated occupancies HDAC1, TRIM28, or NURD subunit CHD4 in non-stressed cells at the AARS/eEF1A genes, with either TRIM28/HDAC1 or CHD4 being observed for all of the genes except the TyrRS gene that shows all three cofactors present. Based on these results, the authors conclude that increased nuclear localization of TyrRS on oxidative stress leads to increased binding of TyrRS to the AARS/eEF1A genes with attendant direct recruitment of either TRM28/HDAC1 or NURD, leading to transcriptional repression of these genes, which is responsible for the reduction in bulk protein synthesis observed after prolonged H2O2 treatment. They go on to provide evidence that cell survival in H2O2 is enhanced by nuclear association of TyrRS (dependent on the NLS), and that in its absence, conferred by the NLS_mut, apoptosis is increased. They also show that ROS increases by preventing TyrRS nuclear localization by the NLS_mut, and that this effect as well as decreased cell survival for this mutant in H2O2 can be rescued by the translation elongation inhibitor harringtonine.

      The results presented in this report provide some support for the main conclusions of the paper and the overall model presented in Fig. 4F. However, as detailed below, many of the main conclusions of the paper are based on correlations and lack direct experimental support, and a number of the experiments are not comprehensive enough with sufficient conditions and controls to establish that the effects observed can be attributed to enhanced nuclear localization of TyrRS in response to H2O2. Considering the statements in the abstract, the evidence is reasonably strong that nuclear localization of TyrRS leads to inhibition of global translation at a stage later than that of eIF2α/ATF4 and mTOR responses, and that excluding TyrRS from the nucleus increases apoptosis under prolonged oxidative stress (although even this last point requires better documentation). However, the evidence is inadequate in several respects to claim that TyrRS directly represses the transcription of translation-related genes by recruiting TRIM28 or NURD complex, and as claimed on p. 13 of the Discussion, that the repression of the four AARS genes and the gene for eEF1A accounts for the reduction in bulk protein synthesis on H2O2 treatment.

      Major issues:

      -Evidence is lacking that the binding of TyrRS to the AARS/eEF1A genes is functionally important for the repression of any of the 6 putative target genes upon increased nuclear localization of TyrRS conferred by the NLS_mut or in response to H2O2. This would require ChIP analysis of TyrRS binding to the target genes for WT vs. NLS_mut TyrRS in H2O2-treated cells; and CRISPR mutagenesis of the putative TryRS binding site in the genome and analysis of transcription in the presence and absence of H2O2 for at least one of the putative TyrRS target genes.

      -Evidence from ChIP analysis is lacking that TRIM28, HDAC1, or the NURD complex are recruited to the AARS/eEF1A genes at native levels of TyrRS in a manner dependent on the NLS and stimulated by H2O2, as the ChIP experiments involved only overexpressed WT TyrRS in non-stressed cells. It is also unclear whether H3K27Ac levels at the putative target genes decline at endogenous levels of TyrRS on treatment with H2O2. Similarly, evidence is lacking that the physical association of TyrRS with these co-repressors is dependent on the NLS and stimulated by H2O2, as the co-IP analysis was limited to endogenous WT TyrRS in non-stressed cells.

      -Evidence is lacking that the cofactors TRIM28, HDAC1, or CHD4 are required for the down-regulation of target gene transcription on H2O2 treatment, which would require knock-down or elimination of these factors by CRISPR accompanied by analysis of target gene transcription +/- H2O2.

      -Direct evidence is lacking from ChIP analysis of RNA Pol II that the transcription of the AARS/eEF1A genes is reduced on H2O2.

      -Evidence is lacking that the repression of bulk protein synthesis is actually mediated by the reduced expression of the 4 AARSs and eEF1A. The fact that the TyrRS-E196K mutation enhances repression of bulk translation and also repression of 3 of the 5 target genes does support the idea that the repression of the target genes is instrumental in reducing protein synthesis, but again, this is still a correlation. There is no evidence that the reduced expression of the AARSs is sufficient to reduce charging of the cognate tRNAs, or that the reduced expression of eEF1A decreases the rate of translation elongation in cells or cell extracts.

      -There is an important lack of information provided needed to evaluate the quality and significance of the ChIP-seq analysis of TyrRS binding to DNA. No details are provided concerning the ChIP-seq analysis of V5-tagged TyrRS to indicate how the TyrRS occupancy peaks were identified and distinguished above background signal from the cells expressing V5 tag alone, whether replicates were examined to provide statistical significance for the identified occupancy peaks, and the sequencing library depths. No genome browser views were provided to show the signals from the cells expressing V5-TyrRS vs V5 alone to demonstrate the quality and reproducibility of data from replicates. The supplementary table S1 describing these data was even omitted from the submission, and it's unclear whether these data are being deposited in GEO.

      -There is an important lack of information provided needed to evaluate the quality and significance of the mass-spec analysis of TyrRS interacting proteins. No details are provided about the statistical significance of the protein interactions identified by mass-spec analysis of the affinity-purified TyrRS; and a negative control for non-specific association seems not to have been included in the analysis. The supplementary table describing these data was even omitted from the submission.

      -It's unclear whether the motif described in Fig. 3A was found under the peaks of TyrRS occupancy in the various genes showing TyrRS binding in the ChIP-seq experiments, nor whether its occurrence is statistically significant. It was not indicated that the motif coincides with the peak ChIP-seq occupancies for TyrRS, and if not, how this could be explained.

      -Evidence is lacking that harringtonine treatment reduced bulk protein synthesis under the conditions where it suppressed the effects of the TryRS NLS mutation in elevating ROS and decreasing cell survival.

      -In general, the figure legends are poorly written in lacking important details about the nature of the TyrRS being examined in the experiment (tagged vs endogenous; overexpressed vs. native levels), and also whether oxidative stress was imposed in the experiment, and if so, the exact conditions for the treatment. Figure legends should contain all of the critical details needed to understand and evaluate the significance of the experimental results without having to search elsewhere in the paper for them.

      -It needs to be clarified whether the mini-TyrRS construct lacks the NLS, and the significance of its behavior as a negative control for the effects of overexpressing WT TyrRS.

      -For the experiment in Fig. 5B, quantification of the fraction of caspase-3 or PARP cleaved from biological replicates is required.

      -The experiment in Supp. Fig. S4 lacks the results from cells untreated with H2O2 to ensure that these proteins were being induced by H2O2 in their hands.

    1. including computer vision, machine vision, speech recognition, natural language processing, audio recognition, social network filtering, machine translation, bioinformatics, drug design, medical image analysis, material inspection and board game programs, where they have produced results comparable to and in some cases surpassing human expert performance<br> yes ma

    1. Your tooltip component will have to wrap your image with a span tag or something, it can’t just add events to its children. And if you are adding multiple actions to it you will have to wrap it multiple times.
      <Concern1> <Concern2> </Concern2> </Concern1>

      vs.

      <img use:concern1 use:concern2>

    1. “Who are you then?" "I am part of that power which eternally wills evil and eternally works good.”

    1. Anti‐Flag monoclonal

      DOI: 10.1096/fj.202001340R

      Resource: (Wako Cat# 018-22381, RRID:AB_10659453)

      Curator: @Naa003

      SciCrunch record: RRID:AB_10659453

      Curator comments: Anti-DYKDDDDK tag Monoclonal Antibody, Unconjugated, Clone 1E6 Wako Cat# 018-22381


      What is this?

    2. anti‐Myc‐tag

      DOI: 10.1096/fj.202001340R

      Resource: (MBL International Cat# 562, RRID:AB_591105)

      Curator: @Naa003

      SciCrunch record: RRID:AB_591105

      Curator comments: Rabbit Anti-Myc Tag Antibody, Unconjugated MBL International Cat# 562


      What is this?

    1. Ich würde das, was du hier diagnostizierst, als Epistemic Crisis bezeichnen. Ich nehme sie genauso wahr wie du, und ich bin auch darüber entsetzt. Das erste Warnsignal, das ich ernst genommen habe, war der Brexit, das zweite die Wahl von Trump. Beide habe ich vorher nicht erwartet, weil sie jenseits des Horizonts waren, in dem ich Entwicklungen erwartet habe. ich muss also auch an der Art und Weise zweifeln, in der ich politische Entwicklungen verstanden habe.—Später kam dann für mich der Aufstieg der Freiheitlichen hier in Österreich, bis hin zur Regierungsbeteiligung, und die rechtspopulistische Welle (wenn man es so nennen will) in Frankreich und Italien.

    1. Your solution is a very basic. The case above is more complex because using your solution you can't manipulate with fetched data outside of template and even outside {#await / } tag. So, if you need a read-only solution it's good but otherwise, it won't help you.
    1. I save the things I read online, too, in a digital research library. I’ve long used Evernote to clip the full text of articles I find and gather them in various digital notebooks, separated into categories for easy reference. I can full-text search everything that I've saved over the past decade, to find the citation really quickly. The combination of my physical library and my note-taking softwares act as a kind of external brain—in other words, my memory gets me to the original source of what I’ve read by searching my notebooks, Evernote, or Pinboard.Recently I’ve been migrating this clip-taking to Pinboard, inspired by a Superorganizers post. Pinboard is much like Evernote, but allows you to tag clipped articles into multiple categories. Pinboard automatically saves a full-text version of each page you clip, so you can search and reference the text even if the website is removed or the page is no longer available. 

      Pinboard, huh? I should take a look at this.

    1. The RFID tag - short for Radio-Frequency Identification - is ubiquitous in the modern economy.

      Bugs