35 Matching Annotations
  1. Jan 2023
  2. Nov 2022
    1. We introduce the use of a specific suite of parameters and protocols that greatly improves the agreement among pipelines developed by diverse organizations.

      From reading the paper it seems like this was done on samples where the SARS-COV-2 in the sample is likely to be genetically uniform. Have you done this at all on samples such as those from wastewater or sewage or environmental swabs where there are likely to be many different variants within a single sample?

  3. Jun 2022
  4. Mar 2022
  5. May 2021
  6. Mar 2021
  7. May 2020
    1. Research on enveloped virus presence and fate in water is hindered by the lack of proven detection methods. Detecting and quantifying viruses in environmental samples requires first concentrating the viruses in the sample into a smaller volume to improve detection limits
    1. The 1MDS-method and the HA-method did not provide as high recovery yields of the virions as the Mg-method.

      The reason for this could be the beef extract used to elute the virions from both these membranes. The other two successful methods (Mg and Al) used H2SO4 for elution.

  8. Apr 2020
    1. Viruses have a direct connection to wastewater and drinking water purification when they are excreted in feces or urine

      How does this compare with spit and nasal secretions which also connect to the wastewater? Is this a bigger source of viral particles in the case of a respiratory virus?

    1. wastewater treatment disease transmission studies from that time did not usually consider respiratory pathogens.

      During the 1980s, there were few pathogens that were both known to initiate infection in the lungs and frequently occur in wastewater (U.S. Environmental Protection Agency, 1980). It was viewed as an anomaly if an enteric pathogen was “uniquely infectious by the aerosol route”, with the noted exception of the respiratory bacterium Mycobacterium tuberculosis

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  9. Mar 2020
  10. Oct 2019
  11. Sep 2019
  12. Aug 2019
  13. Feb 2019
  14. Oct 2017