32 Matching Annotations
  1. Feb 2023
    1. DNA fragmentation by restriction digestion prior to dropletgeneration enables optimal accuracy by separating tandemgene copies, reducing sample viscosity, and improving templateaccessibility for input samples >66 ng per well

      NEB says

      Digestion is recommended whenever DNA input is greater than 75 ng Source

      NEB says that biorad recommends these enzymes: AluI, CviQI, HaeIII, HindIII-HF, MseI

      More guidelines

      • Assemble ddPCR reactions at room temperature, this allows the restriction enzyme to digest the gDNA during the reaction set-up period
      • Prepare reaction mixes as you would for a standard ddPCR reaction. Add 0.5–1 μL of each restriction enzyme (5–20 units, depending on enzyme concentration) to the reaction mixture
      • After set-up, simply continue droplet generation as normal
      • Restriction enzyme will be inactivated during first PCR denaturation step
  2. Nov 2022
    1. For those shorter than 25 bases, a standard (dual labeled) BHQ™ Probe may be perfectly satisfactory.

      Nova might be better to have for 1-step ddPCR applications where the BHQ quenchers are unstable due to reduction of the N=N azo bond by the DTT additive

  3. Jan 2022
    1. Angel, D. M., Gao, D., DeLay, K., Lin, E. Z., Eldred, J., Arnold, W., Santiago, R., Redlich, C., Martinello, R. A., Sherman, J. D., Peccia, J., & Godri Pollitt, K. J. (2022). Development and Application of a Polydimethylsiloxane-Based Passive Air Sampler to Assess Personal Exposure to SARS-CoV-2. Environmental Science & Technology Letters. https://doi.org/10.1021/acs.estlett.1c00877

  4. Nov 2021
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  8. Oct 2020
    1. Landi, F., Marzetti, E., Sanguinetti, M., Ciciarello, F., Tritto, M., Benvenuto, F., Bramato, G., Brandi, V., Carfì, A., D’Angelo, E., Fusco, D., Lo Monaco, M. R., Martone, A. M., Pagano, F., Rocchi, S., Rota, E., Russo, A., Salerno, A., Cattani, P., … Bernabei, on behalf of the G. A. C.-19 G. T. (n.d.). Should face masks be worn to contain the spread of COVID-19 in the postlockdown phase? Transactions of The Royal Society of Tropical Medicine and Hygiene. https://doi.org/10.1093/trstmh/traa085

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  14. Apr 2020