44 Matching Annotations
  1. Jan 2024
    1. a kanamycin resistance gene known to be expressed in both Gram-negative and Gram-positive bacteria

      aphA-3 gene from Campylobacter coli. Ref 21, 25 ~ quoted

      A 1427-bp DNA fragment containing the kanamycin resistance gene, aphA-3, of plasmid pIP1433 from Campylobacter coli was inserted into a shuttle vector. Full expression of aphA-3 was obtained in Bacillus subtilis and in Escherichia coli.

  2. May 2023
  3. Nov 2022
    1. I also think being able to self-host and export parts of your data to share with others would be great.

      This might be achievable through Holochain application framework. One promising project built on Holochain is Neighbourhoods. Their "Social-Sensemaker Architecture" across "neighbourhoods" is intriguing

  4. Oct 2022
    1. I realized that SEO was the best growth strategy for the tool. I created content that targeted specific keywords and published it. It took Google at least 6 months for them to start ranking the content which is when we started experiencing organic sign ups.
  5. Aug 2022
  6. Apr 2022
    1. Caroline Orr Bueno, Ph.D. (2021, September 14). Right-wing radio host Bob Enyart—A staunch anti-vaccine, anti-mask, anti-abortion, anti-gay “firebrand” who used to mock the deaths of people with AIDS — just died of COVID-19. He is the 5th right-wing radio host to die of COVID in the past 6 weeks. Https://t.co/NlKodFEKNB [Tweet]. @RVAwonk. https://twitter.com/RVAwonk/status/1437634022469996550

  7. Dec 2021
    1. what companies want is different than what individuals want companies want an invoice companies want something tax deductible 00:24:34 companies want someone that is um keeping the lights on and that is responsive via email so you really have those obligations and one platform that helps with that is open collective 00:24:47 on open collective you can maybe put that burden of being a fiscal host to the maintainers of open collective they have a 501c6 program for 00:25:00 open source projects that acts as a fiscal host which means they will hand do all the invoicing and they will officially be the maintainers and if you as just an open source 00:25:14 maintainer often or a contributor of that project you want to get money from your project you have to send an invoice to open collective and i think that's the best of both worlds again 00:25:26 because it's very transparent progress a process companies are in the loop and you don't have to deal with all the financial stuff at least not yeah 00:25:39 with with companies

      Open Collective offers a valuable service to those organizations which normally fall between the cracks because, for a plurality of reasons, have not formed a normal legal structure. It provides them with the finanicial instruments normally only available to legal entities to accept funding that they normally would not otherwise have access to.

  8. Jul 2021
  9. Jun 2021
  10. Apr 2021
  11. Feb 2021
  12. Jan 2021
    1. RSF1010 is maintained at a copy-number of about 10-12 per chromosome in E coli, P. aeruginosa and S. enterica sv.Typhimurium (Frey and Bagdasarian, 1989)
    1. This work provides CasTn as a new method for host-independent, programmable, targeted DNA insertions to expand the genomic engineering toolbox.
  13. Dec 2020
    1. Using the Hypothesis API¶

      It'd be great if there were a guide for spinning-up a self-hosted version of the API/Backend

    1. The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica

      pBBR1

  14. Nov 2020
    1. Among the fully sequenced BHR plasmids, most are classified as the well-known incompatibility groups, such as IncP-1 (41 plasmids), IncW (5 plasmids), and IncU (4 plasmids), based on the backbone genes
    1. obviously it's too late, but it's a good practice to keep the 3rd party dependencies mirrored in your own infrastructure :) There is NO GUARANTEE that even a huge site (like launchpad for downloading DEBs) won't go down over a period of time.
  15. Oct 2020
    1. that does not mean that I am advocating the other extreme–i.e., a templating language that allows a lot of logic. I find such templating languages, especially those that allow the host programming languages to be used inside the template, to be hard to read, hard to maintain, and simply a bad choice.
  16. Sep 2020
    1. This specification defines the ::part() pseudo-element on shadow hosts, allowing shadow hosts to selectively expose chosen elements from their shadow tree to the outside page for styling purposes.
  17. Aug 2020
  18. Jul 2020
  19. Jun 2020
  20. May 2020
    1. When an instance is stopped and restarted, the Host affinity setting determines whether it's restarted on the same, or a different, host.

      host affinity setting helps for manage dedicated hosts

    1. Mozilla does not permit extensions distributed through https://addons.mozilla.org/ to load external scripts. Mozilla does allow extensions to be externally distributed, but https://addons.mozilla.org/ is how most people discover extensions. The are still concerns: Google and Microsoft do not grant permission for others to distribute their "widget" scripts. Google's and Microsoft's "widget" scripts are minified. This prevents Mozilla's reviewers from being able to easily evaluate the code that is being distributed. Mozilla can reject an extension for this. Even if an extension author self-distributes, Mozilla can request the source code for the extension and halt its distribution for the same reason.

      Maybe not technically a catch-22/chicken-and-egg problem, but what is a better name for this logical/dependency problem?

  21. Apr 2020
    1. The host responds to the biofilms by mobilizing macrophages and neutrophils to the biofilm area, resulting in secretions of high levels of reactive oxygen species and proteases that can cause damage to the normal and healing tissue. The resulting increase in exudate production provides a source of nutrients to the biofilms, leading to more resistance to healing.
  22. Mar 2020
    1. How do you leverage browser cache when Google’s very own Analytics.js has it’s expiry time set to 2 hours? How do you minimize DNS requests when Google advices you to copy their tracking code, linking to an externally hosted Javascript file?If that isn’t bad enough already, Google’s advice is to avoid hosting the JavaScript file locally. And why? To ensure you get access to new features and product updates.
    2. Why should I host analytics.js locally?The Complete Analytics Optimization Suite for WordPress gives you the best of both worlds. After activation it automagically downloads the latest version of analytics.js from Google’s servers, places the necessary tracking code in your WordPress theme’s header and keeps the local Javascript file up-to-date using an adjusted version of Matthew Horne’s update analytics script and wp_cron(). This way you can minimize DNS requests, leverage browser cache, track your visitors and still follow Google’s recommendation to use the latest features and product updates.
  23. Feb 2020
    1. The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

      Does the host range of transfer go wider than the population where GFP is observed?

      This is my idea of distinctive overlapping categories of host range as venn diagrams.

  24. Jan 2020
    1. Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism
  25. Oct 2019
    1. RP4 has been shown to exhibit a very broad host range and has been demonstrated to efficiently transfer in activated sludge (22, 40), chicken manure (24), and the rhizospheres of various plants (38, 39).
  26. Feb 2019
  27. Dec 2015
    1. The appearance of the cyborg has engendered a newwaveof fear and trepidation towards the invasion of the body by strange technologiesthat threaten to eliminate or overwhelm the human subject

      It sounds like we're creating our own aliens and then essentially putting them inside of a subject/form that we recognize and are quite familiar with so our initial response to the subject will be favourable.. but we're being tricked.. overpowered.. Has anyone read The Host by Stephanie Meyer? Similar concept...