RP4 plasmid (also known as RK2, RP1, and the Birmingham plasmid) stands not only as a model of bacterial conjugation studied over the past 40 years, but also as one of the most conspicuous, broad-host range conjugative plasmids described in the literature. It mediates mating and plasmid transfer between a wide variety of Gram– donors/recipients (8) and is also capable of efficiently conjugating with Gram+, (9) yeast (10,11) and mammalian cells. (12)
68 Matching Annotations
- Dec 2024
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pubs.acs.org pubs.acs.org
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www.mdpi.com www.mdpi.com
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Some conjugation systems, such as IncF, IncH, and IncI plasmids, transfer DNA efficiently in liquid media, while others, including the IncN, IncM, IncP, and IncW plasmids, achieve higher DNA transfer frequencies on solid media [28]. It is suspected that the ability to transfer DNA in different environmental conditions is related to variation in pilus formation, structure, and stability of cells during the conjugation process.
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- Nov 2024
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Local file Local file
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Desmond, Matthew. Poverty, by America. 1st ed. New York: Crown, 2023. https://amzn.to/40Aqzlp
Annotation URL: urn:x-pdf:eefd847a2a1723651d1d863de5153292
Alternate annotation link: https://jonudell.info/h/facet/?user=chrisaldrich&max=100&exactTagSearch=true&expanded=true&url=urn%3Ax-pdf%3Aeefd847a2a1723651d1d863de5153292
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- Poverty, by America
- Matthew Desmond
- Earned Income Tax Credit (EITC)
- poverty
- zoning laws
- Dan Allosso Book Club
- housing market
- landlords
- sociology
- work
- universal basic income (UBI)
- Mollie Orshansky
- taxes
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- Dan Allosso Book Club 2024-11-09
- policy
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- capitalism
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- Apr 2024
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www.vox.com www.vox.com
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“The crux of the issue is we’re thinking about the focus on encampment closure without access to housing,” said Charley Willison, a Cornell professor who has studied the influence of police on cities’ homelessness policies.
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theconversation.com theconversation.com
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Children are struggling to master the most basic reading skills in their home language in the foundation phase (grades 1-3).
My app addresses this I hope - or assists in some way to address this.
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en.wikipedia.org en.wikipedia.org
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Institute in Basic Life Principles (IBLP)<br /> https://en.wikipedia.org/wiki/Institute_in_Basic_Life_Principles
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- Mar 2024
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archive.org archive.org
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https://archive.org/details/run-de-1986-10/page/120/mode/2up
"RUN – Unabhängiges Commodore Computermagazin", Ausgabe 10/Oktober 1986, which has a hexdump code listing of a C64 Zettelkasten
ᔥ[Michael Gisiger[]] in mastodon: (@gisiger@nerdculture.de)
Lust auf #Retrocomputing und #PKM mit einem #Zettelkasten? Bitte schön, in der Oktober-Ausgabe 1986 des #Commodore Magazins RUN findet sich ein Listing für den #C64 dazu. Viel Spass beim Abtippen 😅
https://archive.org/details/run-de-1986-10/page/120/mode/2up
See additional conversation at: https://www.reddit.com/r/c64/comments/1bg0ja1/does_anyone_have_the_zettelkasten_program_from/?utm_source=share&utm_medium=web2x&context=3
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- Jan 2024
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bcbasicincomepanel.ca bcbasicincomepanel.ca
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- Sep 2023
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www.euronews.com www.euronews.com
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“In a few months’ time, this government will not be accountable for the severe consequences that may follow from the Schiphol decision, particularly with respect to relations with the Netherlands’ trading partners, and lost jobs and prosperity at home,”
- for: KLM cap, air travel cap, flight cap, degrowth
- comment
- “In a few months’ time, this government will not be accountable for the severe consequences that may follow from the Schiphol decision, particularly with respect to relations with the Netherlands’ trading partners, and lost jobs and prosperity at home,”
- This comment ONLY refers to things economic, and NOTHING to climate boiling, which air travel is a significant contributor to.'
- If they saw it coming from years ago, why did they not adapt? It is their failure to adapt itself that places themselves in a self-created position of vulnerability
- During a transition as unprecedented as this, the governments of the world must invoke policy that gives protection to workers in industries such as the airline industry and all industries downstream of it so that they can survive the transition as such jobs vanish or morph.
- Indeed, this is one of the major tenets of degrowth advocates. A Universal Basic Income and job retraining to sustainable jobs is the responsible thing to do to protect from job losses.
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- Aug 2023
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danallosso.substack.com danallosso.substack.com
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Remember ChatGPT? It is going to do to the white collar world what robotics and offshoring did to blue collar America. So maybe this isn't the best time to be abandoning the Humanities to focus on vocational training?
This is one of the things that doesn't seem to be being explored enough presently, or at least I'm not seeing it outside of the SAG and WGA strikes where it seems to be a side issue rather than a primary issue.
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- Jul 2023
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davidkorten.org davidkorten.org
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Labor in a fully func-tioning Ecological Civilization will include three essentialelements.
- for:UBI, universal basic income
- for: UBI, universal basic income, futures
- The physical labor required to maintain life’s essential conditions against the forces of entropy.
- The intellectual labor required to constantly test and advance the individual and collective maps of our ever-evolving territory.
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The spiritual labor required to continuously renew our sense of individual and collective connection to all that is.
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comment
- two of these are articulating the entanglement of the individual and collective.
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- Dec 2022
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forum.zettelkasten.de forum.zettelkasten.de
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I'll make the libertarian argument here: Everybody has the right to mess up his or her own life.
The libertarian credo: Everyone has the right to mess up their own life.
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- Jul 2022
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bafybeiac2nvojjb56tfpqsi44jhpartgxychh5djt4g4l4m4yo263plqau.ipfs.dweb.link bafybeiac2nvojjb56tfpqsi44jhpartgxychh5djt4g4l4m4yo263plqau.ipfs.dweb.link
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Let us briefly discuss three specific examples of concepts that seem particularly promising for theprospect of ‘good enough world’ and could become synergistically interrelated: (a) the social policy ofunconditional basic income, (b) the development of blockchains and (c) the idea of the offer networks
!- claim : examples of a good enough world * Universal Basic Income (UBI) * Blockchain * Offer network
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he innovation must modulate the behaviour of the decision-produced socialorganization such that this will result in the realisation of the ‘good enough’ relationship betweenhumans and social systems, that is, it will secure the organic and psychological continuity of thehuman being unconditionally and specifically, irrespective to the continuity of their personware.
!- in other words : enoughness * Universal Basic Income (UBI)
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bbcmic.ro bbcmic.ro
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- Jun 2022
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davidepstein.com davidepstein.com
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WHY GENERALISTS TRIUMPH IN A SPECIALIZED WORLD “The most important business — and parenting — book of the year.” — Forbes “The most important business — and parenting — book of the year.” — Forbes “The most important business — and parenting — book of the year.” — Forbes “The most important business — and parenting — book of the year.” — Forbes “The most important business — and parenting — book of the year.” — Forbes ‹›
Many university presidents site the value of basic research to fuel the more specialized research spaces.
Example: we didn't have any application for x-rays when their basic science was researched, but now they're integral to a number of areas of engineering, physics, and health care.
What causes this effect? Is it the increased number of potential building blocks that provide increased flexibility and complexity to accelerate the later specializations?
Link this to: https://hyp.is/-oEI3OF5EeybM_POWlI9WQ/www.maggiedelano.com/garden/helpful-books
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- May 2022
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www.bbcbasic.co.uk www.bbcbasic.co.uk
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- Dec 2021
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www.theatlantic.com www.theatlantic.com
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what they see as our three basic freedoms: the freedom to disobey, the freedom to go somewhere else, and the freedom to create new social arrangements?
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- Nov 2021
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www.latimes.com www.latimes.com
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Skimmed opening paragraphs in physical newspaper. Want to revisit. This sounds like the sort of "pulling oneself up by one's bootstraps" that actually works.
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- Feb 2021
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www.coursera.org www.coursera.org
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Attribution requires knowledge of two facts: who holds the asset, and who has created it and is party to the contract.
Basic functions of blockchain: Attribution
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psyarxiv.com psyarxiv.com
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Prime, H., Wade, M., May, S., Jenkins, J., & Browne, D. (2021). The COVID-19 Family Stressor Scale: Validation and Measurement Invariance in Female and Male Caregivers. PsyArXiv. https://doi.org/10.31234/osf.io/7328w
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- responsibility
- stress
- disruption
- mental health
- social
- global
- survey
- need
- USA
- caregiver
- family
- parent
- social disruption
- wellbeing
- COVID-19
- parental mental health
- Canada
- career
- UK
- welfare
- family stress
- child mental health
- lang:en
- finance
- is:preprint
- education
- child
- Australia
- basic need
- pandemic
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psychclassics.yorku.ca psychclassics.yorku.ca
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he infants clung to these pads and engaged in violet temper tantrums when the pads were removed and replaced for sanitary reasons.
By determining the subject's need for outside comfort can attest to the use of more that just need of basics like food and vitamins to survive. Using other senses like scent and touch as a connection to something outside of themselves is an important find.
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- Oct 2020
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medium.com medium.com
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But the vast majority of things that our apps are doing are just conditional and list rendering.
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- Jul 2020
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www.theregister.com www.theregister.com
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"AOO is not, and isn't designed to be, the 'super coolest open source office suite with all the latest bells and whistles,'" Jagielski continued. "Our research shows that a 'basic,' functional office suite, which is streamlined with a 'simple' and uncluttered, uncomplicated UI, serves an incredible under-represented community.
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www.nature.com www.nature.com
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Arnold, Carrie. ‘Pandemic Speeds Largest Test yet of Universal Basic Income’. Nature, 10 July 2020. https://doi.org/10.1038/d41586-020-01993-3.
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- Jun 2020
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medium.com medium.com
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Morey, R. D. (2020, June 12). Power and precision. Medium. https://medium.com/@richarddmorey/power-and-precision-47f644ddea5e
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www.tutlane.com www.tutlane.com
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Visual Basic (VB) is an object-oriented programming language and that enables the developers to build a variety of secure and robust applications that run on the .NET Framework.
Visual Basic (VB) is an object-oriented programming language and that enables the developers to build a variety of secure and robust applications that run on the .NET Framework.
To learn more about visual basic refer Visual Basic (VB.NET) Tutorial
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psyarxiv.com psyarxiv.com
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Nettle, D., Johnson, E., Johnson, M., & Saxe, R. (2020). Why has the COVID-19 pandemic increased support for Universal Basic Income? [Preprint]. PsyArXiv. https://doi.org/10.31234/osf.io/csr3u
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- Apr 2020
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psyarxiv.com psyarxiv.com
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Cantarero, K., van Tilburg, W. A., & Smoktunowicz, E. (2020, April 16). Affirming Basic Psychological Needs Promotes Mental Well-Being During the COVID-19 Outbreak. https://doi.org/10.31234/osf.io/pyhce
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www.thelancet.com www.thelancet.com
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Nay, O. (2020). Can a virus undermine human rights? The Lancet Public Health, 0(0). https://doi.org/10.1016/S2468-2667(20)30092-X
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sciencebusiness.net sciencebusiness.net
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Interview: We should tackle COVID-19 head on, but now is not the time to cut basic research. (n.d.). Science|Business. Retrieved April 22, 2020, from https://sciencebusiness.net/news/interview-we-should-tackle-covid-19-head-now-not-time-cut-basic-research
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This principle equally applies to the laws of our country; we should never violate basic rights even if the consequences aren’t immediately evident.
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- Jun 2019
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docs.ethhub.io docs.ethhub.io
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EthHub
Resource for basic, accurate, distilled information about Ethereum.
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- Apr 2019
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Mahatma Gandhi, the father of our nation was a very benevolent and expert political master and observer that he had a great vision for the country. He was a man who fought for the upliftment of the poorer classes of society and thought of reforms in their favour.
True, but still we are not in right track since 1947 !
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- Feb 2019
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Local file Local file
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Is the freedom of the individual served by neoliberalism? Centrality of the state for this freedom, which NL denies. “neoliberal thinkers deliberately sustain the fiction that ‘the market economy’ is a natural and spontaneous order that must be placed beyond politics … The question of how authority can be something other than domination and private power shaped the ideas and action of those who built the tradition of constitutional democracy in western societies from the 16th to the 20th centuries … basic needs were those that had to be met before the individual could practically enact the status of a free subject or person. It was such needs provision that made it possible for individuals to be both personally secure and to enjoy an equality of opportunity to develop as individuals free to discover their talents and gifts … the representation of market society as a spontaneous order is pitched to the punters while, within the tent of the doctrine’s initiates, it is fully understood that the state has to be both a strong state, and to be re-engineered in order to impose neoliberal institutional design.” YeatmanFreedom.pdf
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- Oct 2018
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www.runoob.com www.runoob.com
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可以使用内建函数 make 也可以使用 map 关键字来定义 Map:
// 声明变量，默认 map 是 nil var map_variable map[key_data_type]value_data_type // 使用make 函数 map_variable := make(map[key_data_type]value_data_type)
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www.runoob.com www.runoob.com
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/* 打印子切片从索引 0(包含) 到索引 2(不包含) */ number2 := numbers[:2] printSlice(number2) /* 打印子切片从索引 2(包含) 到索引 5(不包含) */ number3 := numbers[2:5] printSlice(number3)
numbers := []int{0,1,2,3,4,5,6,7,8}len=2 cap=9 slice=[0 1]
len=3 cap=7 slice=[2 3 4]
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通过内置函数make()初始化切片s,[]int 标识为其元素类型为int的切片
s := make([]int, len, cap) -
初始化切片s,是数组arr的引用
s := arr[:]
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www.runoob.com www.runoob.com
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/* 未定义长度的数组只能传给不限制数组长度的函数 */ setArray(array) /* 定义了长度的数组只能传给限制了相同数组长度的函数 */ var array2 = [5]int{1, 2, 3, 4, 5}
func setArray(params []int) { fmt.Println("params array length of setArray is : ", len(params)) }func setArray2(params [5]int) { fmt.Println("params array length of setArray2 is : ", len(params)) }
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www.runoob.com www.runoob.com
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注意：以上代码中倒数第二行的 } 必须要有逗号，因为最后一行的 } 不能单独一行，也可以写成这样：
a = [3][4]int { {0, 1, 2, 3} , {4, 5, 6, 7} , {8, 9, 10, 11} , // 此处的逗号是必须要有的 }上面代码也可以等价于
a = [3][4]int { {0, 1, 2, 3} , {4, 5, 6, 7} , {8, 9, 10, 11}}
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www.runoob.com www.runoob.com
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func getSequence() func() int { i:=0 return func() int { i+=1 return i } }
闭包，A函数 返回一个函数，假设返回的函数为B，那么函数B，可以使用A函数中的变量
nextNumber := getSequence()
nextNumber 是 返回函数B类型，func() int，i是函数A中的变量，初始值 i=0
执行nextNumber()，i+=1, reuturn i ==> 1
再执行nextNumber()，i+=1，return i ==> 2
再执行nextNumber()，i+=1，return i ==> 3
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www.runoob.com www.runoob.com
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for 循环的 range 格式可以对 slice、map、数组、字符串等进行迭代循环。
for key, value := range oldMap { newMap[key] = value }
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www.runoob.com www.runoob.com
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以下描述了 select 语句的语法
- 每个case都必须是一个通信
- 所有channel表达式都会被求值
- 所有被发送的表达式都会被求值
- 如果任意某个通信可以进行，它就执行；其他被忽略。
- 如果有多个case都可以运行，Select会随机公平地选出一个执行。其他不会执行。
否则：
1. 如果有default子句，则执行该语句。 2. 如果没有default字句，**select将阻塞，直到某个通信可以运行**；Go不会重新对channel或值进行求值。
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www.runoob.com www.runoob.com
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iota 表示从 0 开始自动加 1，所以 i=1<<0, j=3<<1（<< 表示左移的意思），即：i=1, j=6，这没问题，关键在 k 和 l，从输出结果看 k=3<<2，l=3<<3。
package main import "fmt" const ( i=1<<iota j=3<<iota k l ) func main() { fmt.Println("i=", i) fmt.Println("j=", j) fmt.Println("k=", k) fmt.Println("l=", l) }以上实例运行结果为：
i=1 j=6 k=12 l=24
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www.runoob.com www.runoob.com
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这种因式分解关键字的写法一般用于声明全局变量
var ( vname1 v_type1 vname2 v_type2 )
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www.runoob.com www.runoob.com
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以一个大写字母开头，如：Group1，那么使用这种形式的标识符的对象就可以被外部包的代码所使用（客户端程序需要先导入这个包），这被称为导出（像面向对象语言中的 public）
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main 函数是每一个可执行程序所必须包含的，一般来说都是在启动后第一个执行的函数（如果有 init() 函数则会先执行该函数）
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- Sep 2018
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www.theatlantic.com www.theatlantic.com
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In deciding when to eat, to work, to sleep, to rise, we stopped listening to our senses and started obeying the clock.
This statement suggests that our society has taken away personal individuality and now expects a certain regimen and pattern in everyones lives.
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- Jul 2018
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Therefore, well controlled basic science studies are important in resolving outstanding fundamental questions regarding means of treatment in a consistent and well established model
Yes, but on the other hand, basic science is expensive and does not provide return on investment. That is not good for the economy and businesspeople.
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- Sep 2017
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www.catholicregister.org www.catholicregister.org
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“We know that poverty is a major driver of ill-health. We also know that poor people trust doctors. It’s a free service. Many other services they won’t access because they worry about the cost,” said Prosper Canada CEO Liz Mulholland.
If only this was an issue in the USA, where going to a doctor is not a free service for the patient, in most cases. The same level of trust is not there; this is unacceptable/
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- Aug 2017
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foramoreperfectamerica.blogspot.com foramoreperfectamerica.blogspot.com
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However, we are willing to work with you on what's really bothering you if you stop behaving like Subhuman sacks of dog shit. Let's fight the influence of big business and Electoral Corruption together. Let's get Universal Basic Income done so not just you, but every American is always secure. Let's end the pointless wars. Let's revitalize and stimulate our inner cities
Voters have been known throughout history, most recently in 2016, to vote against their own interests for reasons that are, frankly, stupid.
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www.basicincomecanada.org www.basicincomecanada.org
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At the core of human rights are the ideals and goals of the “four freedoms” articulated by US President Franklin Delano Roosevelt—freedom of speech, freedom of worship, freedom from fear, and freedom from want—and also the primary goal of self-determination. To achieve these conditions, it is also understood that while all rights are “interrelated, interdependent and indivisible,” the absolute basics of life (e.g., water, food, clothing, and shelter) must first be met.
Canada gets it. The US does not. This must change and quickly.
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- Jun 2017
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documents-dds-ny.un.org documents-dds-ny.un.org
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Characteristics of a full basic income9.In its comprehensive and ideal form, a basic income is explicitly designed to challenge most of the key assumptions underpinning existing social security systems. Rather than a system where there are partial payments, basic income guarantees a floor; instead of being episodic, payments are regular; rather than being needs-based, they arepaid as a flat rate to all; they come in cash, rather than as messy in-kind support; they accrue to every individual, rather than only to needy households; rather than requiring that various conditions be met, they are unconditional; rather than excludingthe well off, they are universal; and instead of being based on lifetime contributions, they are funded primarily from taxation. And simplicity of design promises minimal bureaucracy and low administrative costs.
This is from a group that has thought this concept through, something US politicians do not seem to be capable of.
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- May 2017
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Today, it would be hard to imagine the world without Wikipedia or Linux, and, yet, society has not recognized those as economic contributions.
As a person who loves Microsoft, but uses open-source software including the ones mentioned, the ability to be rewarded for doing the right thing is an universal appeal.
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- Mar 2017
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static1.squarespace.com static1.squarespace.com
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inventing the narratives that are our lives.
Whole intro seems very poetic to me since he is stating that life itself is merely a narrative being told throughout our lives. Kind of feels like the base of every basic tattoo I've seen that say, "I'm the author of my own story" and I've seen about four of those.
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scalar.usc.edu scalar.usc.edu
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Acknowledging prior learning
"Students come to the classroom with preconceptions about how the world works. If their initial understanding is not engaged, they may fail to grasp the new concepts and information that are taught, or they may learn them for purposes of a test but revert to their preconceptions outside the classroom". How People Learn
Examples: Harvard graduates talk about: seasons electricity mass of trees 3:50
"A critical feature of effective teaching is that it elicits from students their preexisting understanding of the subject matter to be taught and provides opportunities to build on—or challenge—the initial understanding."
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Metacognition activities in support of metacognitive practices
This is a basic principle from the researh presented in How People Learn
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Formative Assessment
Another Basic Principle from learning research.
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Community Centered
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- Feb 2017
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static1.squarespace.com static1.squarespace.com
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CHAPTER Vll
Chapter VIII: Just Take What Chapter VII Said About General Audience Awareness and Use Your Discretion to Make It Specific to More Particular Audiences.
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- Jan 2017
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www.dailymail.co.uk www.dailymail.co.uk
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He explained millennials grew up in an environment where 'every child wins a prize' only to find the 'real world' after school is much different.
Do the first several sentences cover all the basic facts of the story: Who, what, when, where, why, how. How are the first several sentences attempting to manipulate the reader?
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- Nov 2016
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essepuntato.github.io essepuntato.github.io
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The language of the article is OK, but I'm afraid it needs some work several places to improve clarity, e.g. by rephrasing or simplification.
Some of the data (the CSV files) has been shared on Figshare and cited as such, but I am missing the raw data of the extracted RDF annotations as well as the scripts used for extraction.
The HTML file of the article in RASH format has for some reason not been submitted as an additional file (only cited by URL) -- perhaps this was not supported by PeerJ's submission system?
The RASH framework and associated software is referenced by GitHub URLs, but without using versioning. For archival purposes and future availability I would appreciate a Zenodo or Figshare archive of a tagged/version of the software, cited using DOI.
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hypothes.is hypothes.is
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Finding tutors can be a really tiring job if not taken in a proper direction. Also, it is equally important to find a competent and knowledgeable tutor, who can provide a proper direction and guidance in your studies rather than just basic teaching. As a student, you should always keep in mind that before hiring a tutor you should have a complete idea of his knowledge about that particular subject.
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- Jun 2016
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Nowhere are there as many bullshit jobs, however, as in Silicon Valley. A survey of 5,000 software developers and engineers last year found that, in the words of The Economist, “many of them feel alienated, trapped, underappreciated and otherwise discombobulated.” Only 19% of tech employees say they are satisfied with their jobs. A mere 17% feel valued. Or, as a former math whiz working at Facebook lamented a few years ago: “The best minds of my generation are thinking about how to make people click ads.”
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- Apr 2016
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The Finnish government is currently drawing up plans to introduce a national basic income. A final proposal won’t be presented until November 2016, but if all goes to schedule, Finland will scrap all existing benefits and instead hand out €800 ($870) per month—to everyone.
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- Nov 2014
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blog.whatfettle.com blog.whatfettle.com
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There’s a certain amount of snobbery about PHP, but I’ve no truck for such snark. PHP is the Web’s BASIC. It’s everywhere, beginner-friendly, and I like it.
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