SciScore rigor report
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Not required = Field is not applicable to this study
Not detected = Field is applicable to this study, but not included.
Ethics
IRB: Samples and data collections were conducted according to the guidelines of the Declaration of Helsinki , and approved by the Ethics Committee Sciences et Santé Animale n°115 ( protocol code COVIFEL approved on 1 September 2020 , registered under SSA_2020_010 ) .
Euthanasia Agents: Cells were then incubated for 72 h at 37 °C with 5 % of CO2 .
Field Sample Permit: These experiments were approved by the Anses/ENVA/UPEC ethic committee and the French Ministry of Research ( Apafis n°24818-2020032710416319 ) .
Consent: All sera from the first cohort , and whole blood samples from the second cohort , were obtained from the Toulouse hospital , where all patients give , by default , their consent for any biological material left over to be used for research purposes after all the clinical tests requested by doctors have been duly completed.
Inclusion and Exclusion Criteria
not detected.
Attrition
One additional conclusion that can be drawn from the comparison of the results of the RBD-ELISA with those of the Jurkat-S&R-flow test is that, whilst the two methods show similar sensitivities, the ELISA signals tend to saturate very rapidly, and are thus much less dynamic that those obtained by flow cytometry.
Sex as a biological variable
Of note, we did not notice an increased frequency of allo-reactivity in samples from women compared to men, which suggests that allo-reactivity after pregnancy is not a major cause in the origin of those allo-reactions.
Subject Demographics
Age: Experiments on virally-infected hamsters Eight week-old female Syrian golden hamsters ( Mesocricetus auratus , strain RjHan:AURA ) from Janviers’s breeding Center ( Le Genest , St Isle , France ) were housed in an animal-biosafety level 3 ( A-BSL3) , with ad libidum access to water and food.
Randomization
The results of the second cohort, which comprised a few Covid patients, but also a large proportion of blood samples randomly picked among those from patients hospitalized for conditions unrelated to Covid-19, yielded a much less clear picture than the first one.
Blinding
On the other hand, the situation was much less clear-cut for the cohort comprising blood samples picked more or less randomly and blindly among those available as left-overs from the hematology department and was, therefore, more akin to a ‘real’ population.
Power Analysis
not detected.
Replication
not required.
Cell Line Authentication
Contamination: The Jurkat-S and Jurkat-R cell lines were both checked for the absence of mycoplasma contamination using the HEK blue hTLR2 kit ( Invivogen , Toulouse , France ).
Authentication: For the same reason , the blood samples for the experiment shown on Figure 3A were collected by one of the authors by simple finger-pricking.