233 Matching Annotations
  1. Last 7 days
  2. Aug 2025
  3. Jul 2025
    1. Reply to u/Charlea1776 at https://old.reddit.com/r/typewriters/comments/1lx86q0/1950_smith_corona_sterling/ on how to mount a typewriter to a typing desk:

      If you're a room or two away, the noise may not be too bad for sleeping children. Typing on a felted wool mat may also be helpful.

      The specially made typing desks made of wood, often had holes drilled into them that allowed one to insert bolts from below that threaded into custom holes in the frame of the typewriter. Generally these only worked with standard machines which were manufactured to be used this way and it's reasonably obvious where the two holes were (usually about midway up the bottom of the frame). When not in use, the top of the desk flips over and hides the typewriter upside down near where one's legs would be positioned. When flipped in typing position, the top of the desk on which the typewriter sits is often lower (25-26" off the floor) than the rest of the desk top (28-29" off the floor).

      I would suspect that your 1950 Sterling, being a portable machine, does not have the requisite mounting holes in the typewriter's chassis for allowing this to happen. Being a portable, these were designed to be put in the case and stowed into a closet or credenza when not in use.

      If you have a later mid-century 20 gauge steel tanker desk, some of those were made with a cabinet section which had a springloaded metal platform which allowed the typewriter to swing up and out of the desk and into position. If this is the type you have, your typewriter might work with this sort of configuration, but these typically didn't have or require mounting hardware like the wooden versions.

  4. Jun 2025
  5. May 2025
    1. To dig deep on this though, .gitignore isn't a standard. It's a well documented and familiar syntax from a specific, widely adopted, tool. Maybe we can even pretend the git implementation is a reference implementation too. There's no spec though and, importantly, it isn't considered a standard by the git maintainers themselves. That's why I kept calling it a quasi-standard in my original post.
    1. There has been an attempt to systematize exit status numbers (see /usr/include/sysexits.h), but this is intended for C and C++ programmers. A similar standard for scripting might be appropriate. The author of this document proposes restricting user-defined exit codes to the range 64 - 113 (in addition to 0, for success), to conform with the C/C++ standard.

      It sounds like he's proposing aligning with the sysexits.h standard?

      But I'm not clear why he refers to "exit codes to the range 64 - 113 (in addition to 0, for success)" as user-defined. To me, these seem the complete opposite: those are reserved for pre-defined, standard exit codes — with 0 for success being the most standard (and least user-defined) of all!

      Why to use exit codes from 1-63 for user-defined errors??

    1. BSD-derived OS's have defined an extensive set of preferred interpretations: Meanings for 15 status codes 64 through 78 are defined in sysexits.h.[15] These historically derive from sendmail and other message transfer agents, but they have since found use in many other programs.[16] It has been deprecated and its use is discouraged.

      [duplicate of https://hyp.is/12j9KjELEfCQc79IbTwQnQ/man.freebsd.org/cgi/man.cgi?query=sysexits&sektion=3 ]

      Why is this deprecated and what should be used instead?? Standardizing this stuff would be good, and this de facto standard seems as good as any!!

  6. Mar 2025
    1. If you're a more serious writer, you're sure to find a smoother and better experience with a standard typewriter, but they're slightly larger and heavier (~30 pounds vs. ~15-22 pounds) than the portables. These are usually the ones I recommend if you're writing for several hours and have a dedicated space for your writing. Standards aren't as popular with most collectors, so they also tend to be less expensive.

      I really love my Royal KMG, HH, and FP and my Remington Standard and Remington 17 which are all serious machines. Here are some of the other more common standards for serious writers: https://www.youtube.com/watch?v=3r3533cSZ38


      Reply to u/RetailThrowAway69 at https://old.reddit.com/r/typewriters/comments/1je4owq/new_typewriter_for_a_keyboard_warrior_of_20_years/

    1. Tested 100s of heavy desk typewriters, Here is the top 5 revealed. by [[Mr & Mrs Vintage Typewriters]]

      Top Five Standard Typewriters:<br /> - Imperial 55 - Imperial 70 (after this they went to cheaper plastic parts); removable carriage, swappable typefaces; removeable core - Hermes Ambassador - removable carriage - Underwood Standards, esp. 5 - Royal 10 (carriage shift); (and subsequent incarnations like the KH, KMM, KMG, HH, FP) - Olympia SG1 (the "crown jewel" of desktop typewriters)

      Also rans: - Olivetti Lexicon, Olivetti Graphica - Contintental m40 & m20 - Mercedes

  7. Dec 2024
  8. Oct 2024
  9. Aug 2024
    1. we are using set theory so a certain piece of reference text is part of my collection or it's not if it's part of my collection somewhere in my fingerprint is a corresponding dot for it yeah so there is a very clear direct link from the root data to the actual representation and the position that dot has versus all the other dots so the the topology of that space geometry if you want of that patterns that you get that contains the knowledge of the world which i'm using the language of yeah so that basically and that is super easy to compute for um for for a computer i don't even need a gpu

      for - comparison - cortical io / semantic folding vs standard AI - no GPU required

  10. Jul 2024
    1. Royal KMM FPE HH KH 10 T1 B64 Typewriter Ribbon Install Rewind Respool Replace by [[Phoenix Typewriter]]

      The spools for the standard Royal typewriters (KMM, FPE, HH, KH, etc) have a custom metal mechanism for their auto-reverse. The spools are known as the T1 (which is the same as General Ribbon part # T1-77B , T1-77BR, and Nu-Kote B64.) If winding on universal ribbon onto them, remove the eyelette which isn't needed and may interfere with the auto reverse.

      The function of the mechanism is fairly similar to that of the Remington, but the mechanism is on the spool itself rather than on the spindle.

      If necessary, Ribbons Unlimited carries these metal spools: https://www.ribbonsunlimited.com/6N064-Royal-Standard-Electric-Ribbon-64-p/6n064.htm

    1. Royal HH & FP typewriter shipping help by [[Typewriter Justice]]

      Advice for how to package a heavy standard typewriter for shipping. Two heavy rubberbands to hold the margin release buttons to protect the escapement. Then plastic wrap to keep the carriage from moving during shipment. Then protection for the knobs and carriage return levers put into a first box. Then packing peanuts in a second box, fill to completely full and tape closed.

    1. Likely the key lockout isn't resetting like it's supposed to upon carriage return - the little part you mentioned. Cleaning and light oil on the friction and pivot points of that and the margin stop assembly usually can get things moving again. That's the most common issue I see in Royal Standards.

      Advice by Nashville Typewriter at https://www.reddit.com/r/typewriters/comments/1dwqi1r/friend_found_in_junk_pile/lbwege9/

  11. May 2024
  12. Apr 2024
    1. Pearl S. Buck and the 1930s RoyalStandard (with white keys) she used towrite The Good Earth, Jack Kerouac’sroad-weary Underwood Standard S,George Orwell’s Remington No. 2,Patricia Highsmith’s Olympia, Marga-ret Mitchell’s Remington No. 3 (whichher husband bought secondhand andshe relied on to type Gone With theWind and countless pieces of corre-spondence with fans).
  13. Mar 2024
  14. Feb 2024
  15. Dec 2023
    1. 仔細聽就可以知道他們講的地地道道的「北京話」和我們所說的「國語」也不是兩回事。當「國語」剩下煮、煨、燉、煎、炒、炸這些「大方向」的動作時,他們還會使用焯、飛水、燒、打沫、擼之類的詞。顏色都還會用棗紅、糖紅、碧綠、翠綠來區分,黏不只黏,還黏糊;顫不只顫,還顫悠。「北京話」和「國語」差異絕對不只兒化音和捲舌問題。問題關鍵當然是在為了方便推行「國語」,人工的、製造的國語便少了很多生活或細節的部份。寫作文章差別愈顯明。平平寫「中文」,「國語」和「北京話」的豐富程度天差地,欲寫贏中國人,真僫。這時台語優勢就出來矣。和北京話仝款,閣保留誠濟用詞幼路的所在,親像炕、燖、煏、𠞭。最近台語文書寫的作家增加也是按呢,使用生活語言寫作,才有可能寫愈好。

      語彙的豐富性,例如煮食

      北京話 >> 普通話, 臺語 >> 國語

  16. Nov 2023
  17. Oct 2023
    1. The giant reserves were then exploited by the behemoths we now know as Getty Oil, ConocoPhillips, Sinclair and Exxon.
      • Conooco before joining with Phillips was a Standard Oil break up and Rockefeller company.
      • Sinclair foundation was funded by Rockefellers
      • Exxon is one of the major Standard Oil off shoots.

      So while the article spends much time attacking Koch, the Rockefellers profited massively from this well before the Koch's came by to scoop up local US supply.

  18. Aug 2023
    1. Undoubtedly the first task of the statesman in such countriesis to raise the standard of living to such a point that thepeople may be freed from economic slavery and given thetime to get the education appropriate to free men.

      A bulk of America was stuck in a form of economic slavery in the 1950s. See description of rural Texans in Robert Caro's LBJ biography for additional context --- washing/scrubbing, carrying water, farming, etc. without electricity in comparison to their fellow Americans who did have it.

      In the 21st century there is a different form of economic slavery imposed by working to live and a culture of consumption and living on overextended credit.

      Consider also the comedic story of the capitalist and the rural fisherman and the ways they chose to live their lives.

  19. Jul 2023
  20. Jun 2023
  21. May 2023
    1. A flaw can become entrenched as a de facto standard. Any implementation of the protocol is required to replicate the aberrant behavior, or it is not interoperable. This is both a consequence of applying the robustness principle, and a product of a natural reluctance to avoid fatal error conditions. Ensuring interoperability in this environment is often referred to as aiming to be "bug for bug compatible".
  22. Mar 2023
    1. Because they follow this specification, most authoritative DNS servers won't allow you to include CNAME records at the root. At CloudFlare, we decided to let our users include a CNAME at the root even though we knew it violated the DNS specification. And that worked, most of the time. Unfortunately, there were a handful of edge cases that caused all sorts of problems.
    1. STANDARD INDEX CARD CO.

      Fascinating to see the 8 various types of hole punches different card index systems may have used on their index card filing cabinets.

      Advertisement from System, December 1906:

      CARD INDEX SYSTEM <br /> If you are using Card Systems, as manufacturers we are in a position to save money for you on these supplies. We make suggestions to anyone desiring to install labor-saving-money- making Card Systems.<br /> Cards supplied for all makes of cabinets.<br /> Write for prices and estimates.<br /> STANDARD INDEX CARD CO.,<br /> 707-09 Arch St., Phila., Pa.

  23. Dec 2022
  24. Nov 2022
    1. It might seem as a preference, but if we have a standardized language for describing software systems, Why do we use something else? This can lead to bad habit of overusing flowcharts. Activity diagrams are really simple. But if you decide to describe a more complicated aspect of the system or try to change the part you are describing, you might have to switch anyway. So just use UML and prevent confusion in the future.
  25. Sep 2022
  26. Jul 2022
    1. L’unità di misura di un valore dovrebbe essere indicata nell’intestazione della colonna. Se l’unità cambia da un valore all’altro, allora bisognerebbe considerare una colonna dedicata con un’opportuna intestazione e non inserire l’unità insieme al valore stesso. Per le unità dovrebbero essere utilizzati i codici (URI) derivati da un vocabolario controllato.

      La commissione economica per l'Europa (UNECE) mantiene un dataset in cui pubblica codici standard per indicare le unità di misura (Standardised codes from Recommendation 20). Il link al dataset è https://datahub.io/core/unece-units-of-measure

  27. Jun 2022
    1. Le intestazioni di colonna devono essere auto-esplicative ed essere incluse nella prima riga del file CSV. Senza le intestazioni, è difficile per gli utenti interpretare il significato dei dati.

      Proprio a integrazione del commento di @aborusso direi che le intestazioni dovrebbero seguire le etichette dei concetti definiti nelle principali ontologie italiane di OntoPiA qualora il dato fosse già modellato. Esempio: "indirizzo completo" (proprietà full address dell'ontologia CLV-AP_IT) per indicare l'indirizzo completo presente in tantissimi dataset (aperti).

    2. cronimo/ abbreviazio ne Titolo Organismo di standardizz azione URL

      In questa tabella c'è un calderone di roba diversa messa sullo stesso piano e questo crea a mio avviso solo confusione. Mi spiego: OWL, SPARQL, RDF, RDFS insieme a RNDT, NUTS e via dicendo è singolare. Inoltre, chissà perché l'assenza di XML a questo punto. Ok che la lista non è esausitva ma non si capiscono i criteri della lista. Intanto OWL, SPARQL, RDF, RDFS sono standard building block per la realizzazione di un nuovo modello di rappresentazione del dato nel web. ONTOPIA, DCAT, DCAT-AP_IT, DataCube ecc. sono tutti standard per la rappresentazione di specifici domini che poggiano (usano) sugli standard dello stack del web semantico prima citati. Suggerimento, mettere in evidenza queste differenze magari anche creando diverse tabelle, indicando i domini applicativi che coprono gli standard (e.g., DataCube per la rappresentazione di SDMX in RDF, quindi contesto dei dati statistici, OntoPiA copre tutta una serie di domini, DCAT metadatazione di dati in cataloghi, EU vocabularies coprono diversi domini ecc.)

  28. May 2022
  29. Apr 2022
  30. Mar 2022
  31. Feb 2022
    1. Hence an email address/mailbox/addr-spec is "local-part@domain"; "local-part" is composed of one or more of 'word' and periods; "word" can be an "atom" which can include anything except "specials", control characters or blank/space; and specials (the *only* printable ASCII characters [other than space, if you call space "printable"] *excluded* from being a valid "local-part") are: ()<>@,;:\".[] Therefore by the official standard for email on the internet, the plus sign is as much a legal character in the local-part of an email address as "a" or "_" or "-" or most any other symbol you see on the main part of a standard keyboard.
  32. Jan 2022
    1. When I think back to the creation of that infographic, I wonder whether we had shown the care demanded of the data. Whether we had, in creating this abstraction, re-enacted — however inadvertently — some of the objectification of the slave trade.

      This sort of objectification seems very similar to the type of erasure that Poland is doing with the Holocaust as they begin honoring Poles who helped Jews while simultaneously ignoring Poland's part in collaborating with the Nazis in creating the Holocaust.

      How can we as a society and humanity add more care to these sorts of acts so as not to continue erasing the harm and better heal past wrongs?

      Cross reference: https://www.nytimes.com/2022/01/29/opinion/holocaust-poland-europe.html and https://hyp.is/hrsb9oIOEey8sEObTYhk0A/www.nytimes.com/2022/01/29/opinion/holocaust-poland-europe.html

    1. Different people have different responses to technology, even on the same platform. Scholars call this phenomenon “differential susceptibility” to media effects among a subgroup of people, and it holds equally for the differential well-being and mental health impacts of social media on young adults.

      Differential susceptibility is a technical term used to describe the ways that different people and different groups have different responses to technology even on the same platform. Similar versions of it can be applied to other areas outside of technology, which is but one target. Other areas include differential well-being and mental health.


      It could also be applied to drug addiction as some are more susceptible to becoming addicted to nicotine than others. Which parts of this might be nature, nurture, culture, etc.

    2. Current approaches to improving digital well-being also promote tech solutionism, or the presumption that technology can fix social, cultural, and structural problems.

      Tech solutionism is the presumption that technology (usually by itself) can fix a variety of social, cultural, and structural problems.

      It fits into a category of problem that when one's tool is a hammer then every problem looks like a nail.

      Many tech solutionism problems are likely ill-defined to begin with. Many are also incredibly complex and difficult which also tends to encourage bikeshedding, which is unlikely to lead us to appropriate solutions.

  33. Dec 2021
    1. When archaeologistsundertake balanced appraisals of hunter-gatherer burials from thePalaeolithic, they find high frequencies of health-related disabilities –but also surprisingly high levels of care until the time of death (andbeyond, since some of these funerals were remarkably lavish).16
      1. Formicola, Vincenzo. 2007. ‘From the Sungir children to the Romito dwarf: aspects of the Upper Palaeolithic funerary landscape.’ Current Anthropology 48: 446–53.

      It will require some investigation, but on it's face this reference to Formicola seems to be about a small number of cases and doesn't point to or back up their claim about high frequencies of societal care and support. Where is their evidence within the archaeological record.

    2. Romito 2 is the 10,000-year-old burial of a male with a raregenetic disorder (acromesomelic dysplasia): a severe type ofdwarfism, which in life would have rendered him both anomalous inhis community and unable to participate in the kind of high-altitudehunting that was necessary for their survival. Studies of hispathology show that, despite generally poor levels of health andnutrition, that same community of hunter-gatherers still took pains tosupport this individual through infancy and into early adulthood,granting him the same share of meat as everyone else, andultimately according him a careful, sheltered burial.15
      1. Tilley, Lorna. 2015. ‘Accommodating difference in the prehistoric past: revisiting the case of Romito 2 from a bioarchaeology of care perspective.’ International Journal of Paleopathology 8: 64–74.

      In a case like this what might have been the reasons for keeping and helping such an individual?

      In an oral society, it's possible that despite his potential physical disabilities for hunting that he may have been an above-average store of knowledge from a memory perspective, thus making him potentially even more valuable to his society.

  34. Nov 2021
    1. I find some of XDG's default dirs, especially ~/.local/share/whatever, to be very annoying. (Almost as annoying as having ~/snap polluting my home dir, but for a different reason.) I shouldn't have to type such long paths or navigate three folders deep in order to access my data files. I therefore make use of the XDG_DATA_HOME environment variable for XDG-style programs, so they will put my files somewhere convenient. However, I don't think Snap can honor that variable, because AppArmor rules require fixed paths. Given 1 & 2, I think ~/.snap/data is a sensible compromise, at least until the underlying components are flexible enough to let the user choose.
  35. Oct 2021
    1. export interface QueueInterface {   count(): number;   dequeue?(): any;   enqueue(...args: any): void;   flush(): any[];   reset(): void;   setFifo(fifo: boolean): void;   setLifo(lifo: boolean): void;   truncate(length: number): void; } export class queue {   protected elements: any[];   protected fifo = true;   constructor(…args: any) {     this.elements = […args];   }   count() {     return this.elements.length;   }   dequeue?(): any {     if (this.fifo) {       return this.elements.shift();     }     return this.elements.pop();   }   enqueue(…args: any) {     return this.elements.push(…args);   }   // Like dequeue but will flush all queued elements   flush(): any[] {     let elms = [];     while (this.count()) {       elms.push(this.dequeue());     }     return elms;   }   setFifo(fifo = true) {     this.fifo = fifo;   }   setLifo(lifo = true) {     this.fifo = !lifo;   }   reset(): void {     this.truncate(0);   }   truncate(length: number) {     if (Number.isInteger(length) && length > -1) {       this.elements.length = length;     }   } } export default queue;
  36. Sep 2021
  37. Aug 2021
    1. What seems more problematic is divergence between drivers. For example, capybara-webkit and poltergeist support several of the same things. Let's take resizing the window as an example. In capybara-webkit this is page.driver.resize_window(x, y) and in poltergeist it's page.driver.resize(x, y). This means that if a user wants to switch from one to the other they have to change their code. Now I don't know if selenium does or doesn't support resizing the window, but supposing it doesn't I think there's still a lot of value in the capybara project deciding what the blessed API is, because then all the drivers that support that feature can implement it using the same API, increasing portability.
  38. Jul 2021
  39. Jun 2021
    1. KV is used in 8 of the overlay locales at the moment (CS, DE, HI, JA, PL, PT, SK, and CN). I don't agree with this and believe that Carmen should only reflect country codes that are part of the actual ISO standard.
  40. May 2021
  41. Apr 2021
    1. The main difference is in the flow of how messages are ultimately sent to devices for output. The standard library Logger logic converts the log entries to strings and then sends the string to the device to be written to a stream. Lumberjack, on the other hand, sends structured data in the form of a Lumberjack::LogEntry to the device and lets the device worry about how to format it. The reason for this flip is to better support structured data logging. Devices (even ones that write to streams) can format the entire payload including non-string objects and tags however they need to.
    1. What you want is not to detect if stdin is a pipe, but if stdin/stdout is a terminal.

      The OP wasn't wrong in exactly the way this comment implies: he didn't just ask how to detect whether stdin is a pipe. The OP actaully asked how to detect whether it is a terminal or a pipe. The only mistake he made, then, was in assuming those were the only two possible alternatives, when in fact there is (apparently) a 3rd one: that stdin is redirected from a file (not sure why the OS would need to treat that any differently from a pipe/stream but apparently it does).

      This omission is answered/corrected more clearly here:

      stdin can be a pipe or redirected from a file. Better to check if it is interactive than to check if it is not.

    1. This question does not show any research effort; it is unclear or not useful Bookmark this question. Show activity on this post. I'm trying to filter the output of the mpv media player, removing a particular line, but when I do so I am unable to control mpv with the keyboard. Here is the command: mpv FILE | grep -v 'Error while decoding frame' When I run the command, everything displays correctly, but I am unable to use the LEFT and RIGHT keys to scan through the file, or do anything else with the keyboard. How do I filter the output of the program while retaining control of it?
    1. I realized it was foolish of me to think the internet would ever pause just because I had. The internet is clever, but it’s not always smart. It’s personalized, but not personal. It lures you in with a timeline, then fucks with your concept of time. It doesn’t know or care whether you actually had a miscarriage, got married, moved out, or bought the sneakers. It takes those sneakers and runs with whatever signals you’ve given it, and good luck catching up.
  42. Mar 2021
    1. My preference here is biased by the fact that I spend everyday at work building web components, so Svelte's approach feels very familiar to slots in web components.

      first sighting: That <template>/<slot> is part of HTML standard and the reason Svelte uses similar/same syntax is probably because it was trying to make it match / based on that syntax (as they did with other areas of the syntax, some of it even JS/JSX-like, but more leaning towards HTML-like) so that it's familiar and consistent across platforms.

    1. the community is both endlessly creative and genuinely interested in solving big issues in meaningful ways. Whether it's their commitment to careful (and caring) community stewardship or their particular strain of techno-ethics, I have been consistently (and pleasantly) surprised at what I've seen during the last twelve months. I don't always see eye-to-eye with their decisions and I don't think that the community is perfect, but it's consistently (and deliberately) striving to be better, and that's a fairly rare thing, online or off.
    1. Will it also help accomplish another goal — communicating to my students that a classroom of learners is, in my mind, a sort of family?

      I like the broader idea of a classroom itself being a community.

      I do worry that without the appropriate follow up after the fact that this sort of statement, if put on as simple boilerplate, will eventually turn into the corporate message that companies put out about the office and the company being a tight knit family. It's easy to see what a lie this is when the corporation hits hard times and it's first reaction is to fire family members without any care or compassion.

  43. Feb 2021
    1. identity theft

      Saw this while scrolling through quickly. Since I can't meta highlight another hypothesis annotation

      identity theft

      I hate this term. Banks use it to blame the victims for their failure to authenticate people properly. I wish we had another term. —via > mcr314 Aug 29, 2020 (Public) on "How to Destroy ‘Surveillance C…" (onezero.medium.com)

      This is a fantastic observation and something that isn't often noticed. Victim blaming while simultaneously passing the buck is particularly harmful. Corporations should be held to a much higher standard of care. If corporations are treated as people in the legal system, then they should be held to the same standards.

    1. Programming to an interface means that when you are presented with some programming interface (be it a class library, a set of functions, a network protocol or anything else) that you keep to using only things guaranteed by the interface. You may have knowledge about the underlying implementation (you may have written it), but you should not ever use that knowledge.
  44. Jan 2021
    1. Great, I can use vw to scale text so it doesn't look puny on a desktop! Perfect... Oh. Huh, now the text is too small to read when viewed on a phone. Okay, well I can just use "max(x,y)" to make sure it doesn't get shrunk beyond a minimum size. Perfect... Oh. Hmm. Looks like "max" isn't supported properly by Chrome. Okay, well guess I'll just use "px" again.
    1. If components gain the slot attribute, then it would be possible to implement the proposed behavior of <svelte:fragment /> by creating a component that has a default slot with out any wrappers. However, I think it's still a good idea to add <svelte:fragment /> so everyone who encounters this common use case doesn't have to come up with their own slightly different solutions.
  45. Dec 2020
  46. Nov 2020
  47. Oct 2020
  48. react-spectrum.adobe.com react-spectrum.adobe.com
    1. However, this would lead to further divergence. Tooling that is built around the assumptions imposed by template literals wouldn't work. It would undermine the meaning of template literals. It would be necessary to define how JSX behaves within the rest of the ECMAScript grammar within the template literal anyway.
  49. Sep 2020
    1. and I keep up with the modern way

      Here we can clearly observe Betteredge's double standard. Just a few lines above, he ascribes a contradictory behavior to women, and now he exhibits it gloriously himself. It's very apparent throughout the story that Betteredge cares a great deal about whether someone is "a Dustman or a Duke." He never fails to talk highly about people of rank, and scarcely about people of no particular hereditary rank. It even manifests itself in the way he values himself (albeit with some insecurity), as opposed to his servants. But here, he so blatantly declares himself free of any prejudicial behavior... This is one of various instances in which his hypocrisy is showing face.

  50. Aug 2020
  51. Jul 2020
  52. Jun 2020
    1. the lowest copy number sample points impacted faster and sronger, then you just need to stabilize by adding a neutral nucleic acid background in your standard curve. I usually use water containig 10ng/microl yeast tRNA to perform serial dilutions. This will first create a reaction background similar to your RTQPCR reaction, but also stabilize your DNA copies; I can freeze and thaw (min 20C) more than 50 times the same standard curve sample without any loss in Cts, from 10E6 to 10E2 copies. When I tested the same standard curve but diluted in water only, then the 10E2 started to be slightly affected after one freeze and thaw and then crashed further; then higher copy numbers samples were also affected after 2 to 3 freeze and thaw.
    1. Slightly on a tangent, but https://github.com/hypothesis/h/issues/777 could be a good target for https://solidproject.org/ to address. The Web Annotation Vocabulary is defined in RDF, so there should be zero overhead.

      yes SOLID would be a neat backend for w3c annotations

  53. May 2020
  54. Apr 2020
    1. a defined quantity of a nonhuman control virus (seal herpes virus [SHV], kindly provided by H. G. M. Niesters, University of Rotterdam, The Netherlands) was spiked into each sample prior to DNA and RNA extraction. Since constant DNA quantities of the control virus are coextracted even when RNA isolation kits are used (H. G. M. Niesters, personal communication; our own unpublished observations), the virus can also serve as a control in RNA virus detection assays.
  55. Mar 2020
  56. Jan 2020
    1. The Web Annotation Data Model specification describes a structured model and format to enable annotations to be shared and reused across different hardware and software platforms.

      The publication of this web standard changed everything. I look forward to true testing of interoperable open annotation. The publication of the standard nearly three years ago was a game changer, but the game is still in progress. The future potential is unlimited!

  57. Dec 2019