for - nonduality - not acting against violence in such a case (as Ukraine war) is an egoic response - acting out of cowardice - Rupert Spira

comment - One can act egoically both to take action AND to not take action.

for - question - Is music worse because entry level is lower? - Musicians response - Bernth's response

for - AI - Security - Open AI statement in response to this essay

for - progress traps - Nora Bateson - response to interviewer's comment on everyday example of complexity - parent encouraging children to go to school - example of mitigating progress traps - complexity is hard!

for - Nora Bateson - response to interview question - Is English language more separating? - Gedanken - Entangled Worlds podcast

response - Nora Bateson - Entangled Worlds podcast question - Is English more separating than other languages? - yes - Gedanken - Nora responds by posing a Gedaken that shows how culturally relative our worldviews are - Our enculturation plays a major role in shaping our worldviews - Ronald Wright's famous quotation about how the human brain has not substantially changed in the past 50,000 years implies that - between the present and anytime less than 50,000 years ago, - if we were transported back in time, we would simply adapt the same culturally norms at that time

epiphany - time travel and a clue to the deepest part of nature within human nature - This Gedanken suggests something important, namely that - if the seemingly immovable worldviews we adopt are a consequence of enculturation - then perhaps that which is the most fundamental aspect of our nature is not dependent on culture? - In other words, if we remove our enculturation, what is left is the most profound set of qualities of being human, - one that transcends all relative cultural perspectives

reference - Ronald Wright computer metaphor on progress traps - Ronald Wright's computer metaphor helps us see how fluid the enculturation of a neonate is - https://hyp.is/6Lb6Uv5NEe2ZerOrftOHfA/www.goodreads.com/work/quotes/321797-a-short-history-of-progress

for - Nora Bateson - response to interview question - Is English language more separating? - loosen up!

for - comparison - complexity - SDG logo vs baby - response - Nora Bateson - to Entangled World podcast interviewer's comment - unintended consequences can be paralyzing

comparison - complexity - Nora Bateson response - SDG logo vs baby - In response to the podcasters's question about how do we act for social change when - it appears that every action can have an unintended consequence? - Nora compares - UN SDG logo with 17 different areas of change - an image of a mother and baby - and she talks about how the image of the mother and baby is so intertwingled that it includes all 17 areas (and probably more)

Students do not expect teachers to be "on call 24/ 7"

response time

research on timing of feedback

research on how 24 period of response shows faculty involvement

Oh awesome can I CITE this? It an online Blog okay because this is great.

key finding - body responses to music are universal

for - BEing journey

The Green Knight shows his excitement to take the hit from Sir Gawain's hand in response to his wanting to take on the challenge. This implies a feeling of loyalty and willingness to carry out their end of the bargain. But then the Green Knight secretly changes the terms of the first agreement struck with King Arthur by adding a new condition to their agreement. The Green Knight adds a sense of mystery as well as potential risk for Gawain when he asks him to vow on his honor to find him later and offer a prize equal to the one he received today.

for - dorsal light response - flying insects attracted to light - light pollution - progress trap - light pollution

for - dorsal light response - flying insects attracted to light - explanation

I see this quote as a direct declaration of war against technology as a whole. Going back to analog roots of brushes, well-worn boots, and a magazine. All things needed in order to set up the grid behind art, a guideline of human creativity.

I feel this rule of the manifesto of futurism still holds up to this day. News, ads, and tabloids come at us faster than ever. There is always something to turn our eyes to and whenever the 15 minutes of fame and attention are up, we move on to the next thing. It is a constant, overstimulating cycle of content.

Each era has their own version of what the world should follow and believe. It is an constant evolving movement.

Pro-violence, very problematic

progressive way of thinking

https://en.wikipedia.org/wiki/Josef_Müller-Brockmann

Josef Müller-Brockmann father of the International Typographic Style and

https://en.wikipedia.org/wiki/Paul_Rand

Paul Rand, who made big scary computers warm and fuzzy

https://www.ibm.com/design/language/ibm-logos/rebus/

https://www.artsy.net/artwork/paul-rand-ibm-think-magazine-eye-bee-m-rebus-cover-1

https://www.moma.org/artists/3569#works

https://helenarmstrong.info

She was "emerging" in 2006 when this was written, nearly 20 years ago. She's still a working professor with interesting projects.

Beyond this question lies another to me. Who is art for? Do artists/graphic designers have a greater responsibility to the people that engage with their art?

It is essential to question what we see as authority just because it looks official. The internet has opened information and platform access to many more people. What that looks like in the future is literally up to us. We can have a great effect as visual artists and strategic thinkers on how culture grows. What will "inclusive" mean 20 years from now. Will we still be fighting the same fights?

Ghana ist weltweit das Land mit der schnellsten Entwaldungsrate. Die meisten Abholzungen sind illegal und widersprechen der neuen EU-Verordnung zu entwaldungsfreien Lieferketten. Die Bestimmungen werden in großen Stil umgegangen, dabei spielt Korruption eine große Rolle, aber auch mangelnde Kontrolle bei den Importeuren. Reportage der taz. https://taz.de/Abholzung-in-Ghana/!5985427/

Share an example of a technology you frequently use and share some of its advantages and disadvantages.

How to beat procrastination?

07.00 Clear goals — goals that focus on the action, not the outcome. (Very specific)

See GTD on next-actions that make a distinction between outcomes (projects) and clear goals (Next-Actions)

"This keeps your brain from wondering, what is the first step?"

10.00 Challenge-skill balance. Find sweet spot where challenge is slightly more than your skill level. Too much challenge is anxiety, too little is boredom. How to tune it? (1) Lower the hurdle. (2) Compress time for a given task. (3) Define scope (What needs to be done? Why? How long?)

14.00 Bypassing/response inhibition. Engaging in a task as soon as you are committed. Don't waver. Sleep to flow is an example.

17.30 Flow payoff — have long blocks of focus, where the struggle to get into flow is actually worth it.

• annotate
• for: response to COP28 president's statement

Die englische Regierung hat in der letzten Oktoberwoche 27 Lizenzen zur Öl- und Gasförderung in der Nordsee vergeben. George Monbiot konfrontiert diese Entscheidung mit aktuellen Erkenntnissen zum sechsten Massenaussterben und dem drohenden Zusammenbruch lebensunterstützender Systeme des Planeten https://www.theguardian.com/commentisfree/2023/oct/31/flickering-earth-systems-warning-act-now-rishi-sunak-north-sea

a large German dirigible airship of the early 20th century, long and cylindrical in shape and with a rigid framework. Zeppelins were used during World War I for reconnaissance and bombing, and after the war as passenger transports until the 1930s.

a violent or sudden change or disruption to something

without embarrassment or shame.

Durch die Entwaldung des Amazonas-Regenwalds steht das südamerikanische Monsunsystem kurz vor einem Kipppunkt, nach dessen Überschreiten die Niederschläge im Amazonasgebiet um 30% sinken und der Regenwald langsam verschwinden würde. Eine neue Studie zeigt, dass dieser Kipppunkt unmittelbar bevorstehen könnte. https://www.theguardian.com/environment/2023/oct/04/south-american-monsoon-heading-towards-tipping-point-likely-to-cause-amazon-dieback

Study: https://www.science.org/doi/10.1126/sciadv.add9973

Under this scenario, a grid operator would pay a datacentre to be prepared to switch off local physical load.

The load would still be served through, albeit on a different grid or grid region where you didn't have the same strains on the electricity network.

What is a teleoperator?

The manifestos focus on technology, efficiency and collective purpose over individualism have echoes today.

The Futurist manifesto is all about collective action and getting aggressive instead of just sitting around thinking. Marinetti aimed to hype up crowds and shake things up.

The rise of communism and the soviet revolution influenced Rodchenko embrace to constructivism.

This is a tool offered by Unicorn.com specifically for energy reporting

Where would I look to find public evidence of this?

• for: polycrisis, polycrisis - response, incrementalism, greenwashing,
• paraphrase
  • our preoccupation with
    • one-upping the Tweedledums, and with
      • social justice’ platitudes,
      • incrementalism,
      • technotopian thinking,
      • niceties of speech, and
      • mistaking
        • virtue signalling,
        • policy platforms, and
        • wishful out-loud thinking
      • for actual progress toward real goals,
      • constitute a
        • grossly negligent and
        • staggeringly ignorant response
      • to the existential crises of the day.

Once a movement becomes mainstream it's no longer sortout by the masses.

This is an example of underground turned mainstream.

The rawness of the avant-garde's talent connects to the public better than a structured sortout slogan. It speaks the language of the public.

Basically the underground talent is too raw to be publicized on its own so it needs to be watered down to be acceptable to become mainstream.

In a musical setting, however, when spoken words and sentences are replaced with motifs and melodic phrases, the structure of the call and response might not be as obvious. To be a good communicator, we have to know how to listen, pay close attention to what the other musicians are playing, and try to be receptive to their ideas. In certain scenarios, however, the use of call and response technique might create less than desirable effects. For instance, when the call and response takes the form of exact and immediate repetition, it might be impressive but not necessarily in keeping with the surrounding musical context. A much more subtle way of thinking about the call and response technique involves musical interaction at the level of the entire performance in which non-adjacent sections relate to one another, and where the flow of the performance is regulated by logically introduced musical ideas. In creating a musical narrative, then, we can also respond to each other’s playing, but these responses are not as obvious as simple repetitions tend to be. We can demonstrate our listening skills, for instance, by incorporating an idea that we have previously heard (i.e. a rhythmic motive from the drummer, or a melodic gesture from the guitarist) and develop it in such a way that leads to a more satisfying musical discourse. The call and response aspect of improvisation means that musicians understand each other’s intentions, have an unspoken agreement, so to speak, and project them with a high level of personal expression and musical commitmen

https://docdrop.org/pdf/Kernfeld---2002---Call-and-Response-lrg5i.pdf/

Call and response Kernfeld, B. 2003

Polyrhythm, call-and-response, and syncopation

[19] AI - Legal Response

springbok tour 1981

This emphasizes the importance of the designer understanding how their work ties into how people determine their level as designers.

The values that this style presented were impactful enough to reach the U.S thus foreshadowing it's importance.

This can relate to their approaches to design and how they may relate to our approaches.

This will be important to keep in mind for later in the reading.

• many experience a = freeze response (fear and inability to act), or indeed a = flight response
• avoiding the problem altogether.
• While it can feel counter-intuitive, meeting climate dismissives with = compassion can go a long way and help them break free from denial.

This reminds me of, during the height of the iPod era, when someone I know was gifted* an non-Apple music player and some iTunes gift cards—their first device for their first music purchases not delivered on physical media. They created an iTunes account, bought a bunch of music on the Music Store, and then set about trying to get it onto their non-Apple device, coming to me when it wasn't going well trying to get it to work themselves. I explained how Apple had (at the time) made iTunes Music Store purchases incompatible with non-Apple devices. Their response was baffling to me:

Rather than rightly getting pissed at Apple for this state of affairs, they did the opposite—they expressed their disdain about the non-Apple MP3 player they were given** and resolved to get it exchanged for credit so they could buy a (pricier, of course) Apple device that would "work". That is, they felt the direct, non-hypothetical effects of Apple's incompatibility ploy, and then still took exactly the wrong approach by caving despite how transparently nefarious it all was.

Returning to this piece: imagine if all that stuff hadn't been locked up in the social media silo. Imagine if all those "comments, posts, and photos from the past 14 years" hadn't been unwisely handed over for someone else to keep out of reach unless you assimilated. Imagine just having it delivered directly to your own inbox.

* NB: not by me

* NB: not as a consequence for mimetic desire for the trendiest device; they were perfectly happy with the generic player before they understood the playback problem

Author response

To: Reviewer: Heikki Vapaatalo, MD, PhD, Emeritus professor of Pharmacolog

Dear reviewer Thank you very much for the insightful suggestions, the manuscript improved a lot with the changes performed. Please find the point-by-point answer to the raised questions. In the main text, all changes are highlighted in yellow. I hope that with the changes made the new version is suitable for publication.

Best regards Valquiria Bueno

General assessment

The study is interesting and the title promises for me more than the MS finally contains.

Answer: The manuscript is part of a project aiming to study ACE1 and ACE2 expression in cells from the immune system of aging and young adults. These initial results suggest that ACE1 (and probably ACE2) plays somehow a role in the process of aging.

The background, question and the aim are relevant as explained in the introduction.

Answer: We included a piece of information in the “Introduction” trying to link ACE1 expression in tissue cells and age-related diseases, as it follows:

ACE1 has been suggested to influence age-related diseases (i.e. Alzheimer’s, sarcopenia, cancer) but the associated mechanisms are still under investigation. ACE1 polymorphisms were correlated with susceptibility to Alzheimer’s disease (AD). [15, 16] In addition, it was shown recently that in normal aging ACE1 expression is increased in brain homogenates and this expression is unchanged in early stages of AD. [17] Regarding sarcopenia, Yoshihara et al. [18] found a weak correlation between ACE polymorphism and physical function. In cancer (gastric or colorectal), patients presented higher expression of ECA1 in tumor when compared with healthy tissues. [19, 20]

The major criticism concerns the small size of the material (subjects, n=6), the small age difference (64-67 years) and the lack of younger controls.

Answer: We agree that the small number of studied subjects is a limitation of this study. In spite of the interesting results suggesting that ACE1 expression could be linked to the health status, it was not possible to perform correlation analysis due to the small sample size. Even though there is a small chronological difference between the subjects, the biological aging is very different among them and reflects the genetics, lifestyle, nutrition and comorbidities. Another limitation is the lack of younger controls to compare with the subjects studied. Our next steps are to include younger controls, to increase the number of studied subjects, and if possible to get samples from older subjects (i.e. 70-80, 80 and more years old)

Minor notes:

1)Title: Angiotensin converting enzyme (ACE) expression in leukocytes of older adults

Answer: We evaluated only ACE1 expression, and thus, title, abstract, and main text were changed to ACE1 instead of ACE. We decided to change to title for: Angiotensin converting enzyme (ACE) 1 expression in leukocytes of adults from 64 to 67 years old

2)Introduction: The last chapter, the Author should explain in more detail, how references 11-14 suggest that “ACE play an important role in the aging process”. Does this mean, that ACE is somehow regulating the aging process or in increasing age ACE -levels are changed?

Answer: References 11-14 shows that age-related diseases occurring in older adults are associated with changes in the immune system. To complete the text we added:

ACE1 has been suggested to influence age-related diseases (i.e. Alzheimer’s, sarcopenia, cancer) but the associated mechanisms are still under investigation. ACE1 polymorphisms were correlated with susceptibility to Alzheimer’s disease (AD). [15, 16] In addition, it was shown recently that in normal aging ACE1 expression is increased in brain homogenates and this expression is unchanged in early stages of AD. [17] Regarding sarcopenia, Yoshihara et al. [18] found a weak correlation between ACE polymorphism and physical function. In cancer (gastric or colorectal), patients presented higher expression of ECA1 in tumor when compared with healthy tissues. [19, 20]

Material and Methods:

The N-value of the subjects should be mentioned here, as well the relation of females/males.

Answer: Text was correct as suggested Blood was collected from adults (n=6, four females and two males) aged 64-67 years old in 2015.

Do the Authors really regard 64-67 “older age” nowadays?

Answer: Nowadays the most common term used for individuals older than 65 years is “older adults”.

Why first many years later the assays have been done in comparison to the collection of the blood? Are the samples still useable, not destroyed?

Answer: Samples are part of UNIFESP Biobank and have been kept in adequate conditions. We wanted to test cells from a period anterior to COVID-19 and those samples were the only ones that attended our purpose. We compared samples used in this study with fresh blood samples (cell viability and percentage of CD4+, CD8+ and CD19+) and the results showed good preservation of the cells.

Did the subjects have some diseases and/or drugs because the possibly were from hospital sample bank?

Answer: Samples are part of UNIFESP Biobank, but unfortunately we do not have information about diseases and medicaments.

Express the company details similarly than Amersham, cities and countries.

Answer: Changes were done as required ACE CD143 FITC (R&D Systems, Inc, Minneapolis, USA)

Results:

“Table 1 shows that older adults…..” The comparison between the present data and historical studies belongs to the Discussion.

Answer: Changes were done as required

Give also individual ages and gender of the subjects in the table 1.

Answer: The manuscript version sent to medrxiv@medrxiv.org had age and gender on tables, but due to their request, any possible variable that could identify the studied individual had to be removed. That is why in the present version these variables are not shown.

What means p-values here? Compared with which or interindividual differences in the particular variable? Should be explained

Answer: We used p-value for interindividual differences in each variable since individuals age differently (biological aging) and thus, physiological parameters could be affected by genetics, lifestyle, nutrition, and comorbidities. It is now explained in materials and methods

The numbering of tables and the text seems to me confusing. Only three tables, but in the text mentioned four. Number 4 does not exist.

Answer: For some reason table 2 is missing in the main text, please find the new version with Table 2 included

It would be good to have a list of abbreviations used in the description of the cell types for an unfamiliar reader.

Answer: In each figure and table we are now providing a description of cells evaluated.

Discussion:

A major part of the discussion deals with previous publications and not meaning or clinical significance of the present findings and comparison between the present and earlier studies.

Answer: The discussion was changed as suggested:

Our results show that for the studied population, chronological aging and biological aging don´t go at the same pace. Even individuals having a small chronological difference (64 to 67 years old), they are heterogeneous for physiological parameters such as glucose, urea, glycated hemoglobin (Hbglic), and C-reactive protein (CRP). Changes in the same functional parameters have been reported by Carlsson et al. [22] and Helmerson-Karlqvist [23] in healthy older adults. Carlsson’s study [22] found that CRP value was 2.6 with a coefficient variation of 1.4% whereas in our study, it was observed higher values of CRP in 5 out of 6 individuals. Increased CRP levels has been associated with inflammaging and our findings show that the studied population has changes in functional parameters which are likely associated with an inflammatory profile. [24] The link between RAS and inflammation has been suggested but its role is not completely clear under physiological and pathological conditions. [25, 26] In addition, the association between ACE1 altered expression in tissues (brain, muscle, heart and vessels) and the development and progression of age-related conditions such as Alzheimer’s, sarcopenia, and cardiovascular disease has been suggested but results are controversial. [17, 27, 28, 29, 30] There are few studies showing the association between ACE1 expression in cells from the immune system (monocytes, T cells) and the progression of kidney and cardiovascular disease. [9, 8, 31, 32]. Therefore, considering the lack of information on this issue, we questioned whether ACE1 (CD143) was highly expressed in cells from the immune system during the aging process. We found that ACE1 was expressed in almost 100% of T (CD4+, CD8+) and B lymphocytes and in all phenotypes of these cells. In non-lymphoid cells, ACE1 mean expression was 56,9%. In agreement with our findings, independent studies showed that T cells from healthy donors and monocytes from patients with congestive heart failure expressed ACE1, but there was no investigation on cell phenotype. [25, 26]. Our study is the first to show that either inexperienced (naive) or fully activated (memory) cells expresses ACE1. Our findings suggest a that the expression of ACE1 in lymphoid and non-lymphoid cells reflects the health status since our studied population presented changes in physiological parameters and high levels of ACE1 expression on immune cells. Previous independent studies showed that patients with unstable angina [32] or acute myocardial infarction [33] presented higher expression of ACE1 in T cells and dendritic cells than controls subjects. In addition, markers of the cell (lymphoid and non-lymphoid) functional status such as inflammatory or growth factors production could be modulated by ACE inhibitors (ACEi). Accordingly, mononuclear leukocytes from healthy subjects incubated with endotoxin exhibited high levels of tissue factor activity which was reduced in the presence of captopril in a dose-dependent pattern. This result could be related to the antithrombotic effect of ACEi. [34]. In patients with congestive heart failure, immune cells cultured with LPS secreted high levels of the pro-inflammatory TNF-alpha and these levels were significantly reduced in the presence of captopril. [35]

In those previous studies, also ACE2 has been reported, why not studied here?

Answer: Our next studies will be focused on ACE1 and ACE2 expression in cells from the immune system in both younger and older adults.

In the limitations, the Authors fairly mention the real problem: The small sample size, and I would like to say lack of younger subjects.

Answer: we agree with the limitations pointed and the text was changed as required:

This study have limitations such as the small sample size and the lack of young adults for comparison. As an example, the subject with the highest CRP and albumin also exhibited a high percentage of ACE1 expression on T (CD4+, CD8+), B and non-lymphoid cells in addition to the lowest percentage of CD4+ naive cells, and the highest percentage of CD8+ terminally differentiated (EMRA) and DN B cells. However, due to the small sample size it was not possible to associate the high expression of ACE1 on immune cells with inflammaging and immunosenescence. It would bring important information to correlate physiological parameters/health status with ACE1 expression and to find out whether age and associated chronic diseases could lead to increased ACE1 expression.

The COVID-19 point even tempting today, is too far from this study and unnecessary. Answer: Our point was to emphasize the negative impact of chronic diseases for the outcome of aging population during a viral infection and how ACE1/ACE2 expression could bring information to diagnosis and treatment. Therefore, we would like to maintain this piece of information.

Linguistic checking would improve the MS. Answer: We checked for possible linguistic mistakes

Reviewer, Heikki Vapaatalo:

I read with pleasure the very detailed answers to my comments.

I very warmly recommend acceptance of this MS for publications without any further notes.

Decision changed:

Verified manuscript: The content is scientifically sound, only minor amendments (if any) are suggested.

To: Reviewer: Calogero Caruso

Dear Prof.Caruso Thank you very much for the revision of this manuscript. It is a privilege to have a manuscript reviewed by a research with high expertise on the field of ageing. Please find the answers to your questions and in the main text the changes in bold.

Sincerely yours,

Valquiria Bueno

The paper is essentially anecdotal because it studies the cells of 6 subjects without any comparison with other age groups. There is also a serious limitation because beyond the age and sex there is no information on the donors (how and why they were recruited, what drugs they took, etc.).

It is really a limitation to have only 6 individuals for the study, but they were the only ones fitting in the proposal of the manuscript. The samples were from a central bank of cells at UNIFESP and participants were considered “healthy” but there was not further information in addition to what we displayed on the tables of the manuscript. They were not living on homecares or hospitalized.

Our aim was to evaluate samples from individuals aged 60-69 years previously to COVID-19 and/or vaccination. In addition, there were no samples in the same conditions (PBMCs, -80oC) of young individuals and using fresh blood could bring a result that could not be compared mainly regarding to myeloid cells and B cells as is follows in the below reference. Braudeau C, Salabert-Le Guen N, Chevreuil J, Rimbert M, Martin JC, Josien R. An easy and reliable whole blood freezing method for flow cytometry immuno-phenotyping and functional analyses. Cytometry B Clin Cytom 2021;100(6):652-665. doi: 10.1002/cyto.b.21994.

Our goal from now on is to expand this study with young and old adults samples since it is important to understand whether ageing is associated with an increase in ACE expression on immune cells.

-To infer that chronological and biological ages do not match is inappropriate in the absence of the above information.

This piece of information regarding chronological and biological age was required by another reviewer. I agree that the concept does not match without more information on the donors. However, the information is now referenced and should be considered when older adults are studied. Vasto S, Scapagnini G, Bulati M, Candore G, Castiglia L, Colonna-Romano G, Lio D, Nuzzo D, Pellicano M, Rizzo C, Ferrara N, Caruso C. Biomarkes of aging. Front Biosci (Schol Ed) 2010;2(2):392-402. doi: 10.2741/s72. PMID: 20036955.

-However, the paper is of some interest because there are few studies on the topic.

Thanks for this positive comment. Few studies on the topic was the reason why we decided to send the manuscript for publication even though there were some important information on the donors missing and limited number of individuals.

Essential revisions that are required to verify the manuscript

1) Although we do not have data on donors, placing an age and gender column in all tables adds a minimum of useful information for the reader.

The first table submitted with age, but for requirement of MedRxiv, gender and age could no be linked to the metabolic results to preserve the anonymity of the donors.

2) Inflamm-ageing means low grade of inflammation. The value of CRP 23.1 suggests acute inflammation (also because albumin has high values, while in chronic inflammation its values decrease). Therefore the Ly averages do not have to take this subject into account.

Thank you for this comment. In a review of literature it was found an article (below) with CRP variation from 0.1 to 19.8 (Heumann Z, Youssim I, Kizony R, Friedlander Y, Shochat T, Weiss R, Hochner H, Agmon M. The Relationships of Fibrinogen and C-Reactive Protein With Gait Performance: A 20-Year Longitudinal Study. Front Aging Neurosci 2022;14:761948. doi: 10.3389/fnagi.2022.761948). There is also an article from your group showing CRPs <5g/dL and >5g/dL (Cancemi P, Aiello A, Accardi G, Caldarella R, Candore G, Caruso C, Ciaccio M, Cristaldi L, Di Gaudio F, Siino V, Vasto S. The Role of Matrix Metalloproteinases (MMP-2 and MMP-9) in Ageing and Longevity: Focus on Sicilian Long-Living Individuals (LLIs). Mediators Inflamm 2020;2020:8635158. doi: 10.1155/2020/8635158) that will be used to discuss how ageing impacts CRP levels. Considering the already small number of donors, data were maintained and statistics (mean + SD) with and without 23.1 mg/dL are now shown.

This will be the new version (discussion) about CRP Carlsson’s study [22] found that CRP value was 2.6 with a coefficient variation of 1.4% whereas in our study, it was observed higher values of CRP in 5 out of 6 individuals. In addition, it was shown by Cancemi et al. in an evaluation of individuals from 40 years to older than 95 years (long-living) that CRP increases in an age-dependent manner. Increased CRP levels has been associated with low grade of chronic inflammation (inflammaging) and our findings show that the studied population has changes in functional parameters which are likely associated with an inflammatory profile. [24] However, an individual presented CPR 23.1 mg/dL suggesting acute inflammation instead, but as all donors were not hospitalized or living on homecares, this sample was considered as part of the study. Another study evaluating gait speed found CRPs varying from 0.1 to 19.8mg/dL (Front Aging Neurosci 2022;14:761948.). Our study has an important limitation that is the lack of data on donors such as the use of continuous medicaments or sarcopenia, hypertension, cognition, among others, and thus it was not possible to correlate CRP with age-related conditions.

Table 1. Updated

Other suggestions to improve the manuscript The authors write that their findings suggest that ACE1 could play a role in several processes linked to aging including the generation and activation of autoimmune cells, due to the experimental evidence that inhibitors of ACE suppress the autoimmune process in a number of autoimmune diseases such as EAE, arthritis, autoimmune myocarditis. [49] They do not appear to have these findings in their paper. So, it needs to change the sentence.

Sentence changed to: According to experimental evidence, ACE inhibitors suppress the autoimmune process in a number of autoimmune diseases such as EAE, arthritis, autoimmune myocarditis. [49] Extrapolating these findings to our results, it is possible to suggest that ACE1 play a role in several processes linked to aging including the generation and activation of autoimmune cells.

Rviewer: Calogero Caruso

Decision changed:

Verified manuscript: The content is scientifically sound, only minor amendments (if any) are suggested.

The mainstream is seen as the standard while the underground is seen as a copy or replica.

Sometimes art and ideas can be considered as less than what already is accepted, as a result of things becoming more simplified and easier, resulting these neglected or irrelevant ideas one day becoming crucial to the future.

This was a great statement because it sparks curiosity in how the world around us is greatly influenced by the past and this which can result in what can be considered "Theft" and copyright infringement when considering how simple it has become to succeed commercially.

Experimentation was something that became increasingly important even though it wasn't supported much at times.

not complient with Problem Details, which requires details to be a string

This relates to the increasing difficulty of being originial with the rapid advances in productivity today.

This relates to sticking to past foundations that are somewhat indispensable.

Today, this is foundational since there are constantly advances in works of typography making documentation and research important to giving future credit.

Clarity becomes less important due the focus on the composition being centralized as it relates to improving the visual appeal of the artistic, "arrangement."

The central axis in art relates to a somewhat balanced distribution of space on an art piece.

“ARTTALK Chapter 10 Balance - Ppt Google Img.” SlidePlayer, https://slideplayer.com/slide/10709291/.

This emphasizes that having clear communication is made crucially important due to the difficulty of communicating to those who are surrounded by countless other "prints."

Lucas, C., Vogels, C. B. F., Yildirim, I., Rothman, J. E., Lu, P., Monteiro, V., Gelhausen, J. R., Campbell, M., Silva, J., Tabachikova, A., Peña-Hernandez, M. A., Muenker, M. C., Breban, M. I., Fauver, J. R., Mohanty, S., Huang, J., Shaw, A. C., Ko, A. I., Omer, S. B., … Iwasaki, A. (2021). Impact of circulating SARS-CoV-2 variants on mRNA vaccine-induced immunity. Nature, 1–9. https://doi.org/10.1038/s41586-021-04085-y

Warner, E. L., Barbati, J. L., Duncan, K. L., Yan, K., & Rains, S. A. (2022). Vaccine misinformation types and properties in Russian troll tweets. Vaccine. https://doi.org/10.1016/j.vaccine.2021.12.040

Munro, A. P. S., Janani, L., Cornelius, V., Aley, P. K., Babbage, G., Baxter, D., Bula, M., Cathie, K., Chatterjee, K., Dodd, K., Enever, Y., Gokani, K., Goodman, A. L., Green, C. A., Harndahl, L., Haughney, J., Hicks, A., van der Klaauw, A. A., Kwok, J., … Appleby, K. (2021). Safety and immunogenicity of seven COVID-19 vaccines as a third dose (booster) following two doses of ChAdOx1 nCov-19 or BNT162b2 in the UK (COV-BOOST): A blinded, multicentre, randomised, controlled, phase 2 trial. The Lancet, S0140673621027173. https://doi.org/10.1016/S0140-6736(21)02717-3 s

Beaumont, P. (2021, December 17). T-cells in Pfizer Covid jab recipients stay robust against severe illness. The Guardian. https://www.theguardian.com/world/2021/dec/17/t-cells-pfizer-covid-jab-robust-against-severe-illness-south-africa-research

Sadoff, J., Gars, M. L., Cardenas, V., Shukarev, G., Vaissiere, N., Heerwegh, D., Truyers, C., Groot, A. M. de, Scheper, G., Hendriks, J., Ruiz-Guinazu, J., Struyf, F., Hoof, J. V., Douoguih, M., & Schuitemaker, H. (2021). Durability of antibody responses elicited by a single dose of Ad26.COV2.S and substantial increase following late boosting (p. 2021.08.25.21262569). https://doi.org/10.1101/2021.08.25.21262569

Thuluva, S., Paradkar, V., Turaga, K., Gunneri, S., Yerroju, V., Mogulla, R., Kyasani, M., Manoharan, S. K., Medigeshi, G., Singh, J., Shaman, H., Singh, C., & Rao, A. V. (2022). Selection of optimum formulation of RBD-based protein sub-unit covid19 vaccine (Corbevax) based on safety and immunogenicity in an open-label, randomized Phase-1 and 2 clinical studies (p. 2022.03.08.22271822). medRxiv. https://doi.org/10.1101/2022.03.08.22271822

Gebre, M. S., Rauch, S., Roth, N., Yu, J., Chandrashekar, A., Mercado, N. B., He, X., Liu, J., McMahan, K., Martinot, A., Martinez, D. R., Giffin, T., Hope, D., Patel, S., Sellers, D., Sanborn, O., Barrett, J., Liu, X., Cole, A. C., … Barouch, D. H. (2021). Optimization of Non-Coding Regions for a Non-Modified mRNA COVID-19 Vaccine. Nature, 1–8. https://doi.org/10.1038/s41586-021-04231-6

Henley, J., & correspondent, J. H. E. (2021, November 19). Majority of public in Europe support Covid vaccine passports – survey. The Guardian. https://www.theguardian.com/world/2021/nov/19/majority-of-public-in-europe-support-covid-vaccine-passports-survey

Feldman, J. (2021, September 25). All the Ways That “1 in 5,000 per Day” Breakthrough Infection Stat Is Nonsense. Slate. https://slate.com/technology/2021/09/breakthrough-infections-one-in-five-thousand-nonsense.html

Zimmer, C. (2021, June 6). We’ll Probably Need Booster Shots for Covid-19. But When? And Which Ones? The New York Times. https://www.nytimes.com/2021/06/06/science/covid-vaccine-boosters.html

Maier, B. F., Wiedermann, M., Burdinski, A., Klamser, P., Jenny, M. A., Betsch, C., & Brockmann, D. (2021). Germany’s current COVID-19 crisis is mainly driven by the unvaccinated (p. 2021.11.24.21266831). https://doi.org/10.1101/2021.11.24.21266831

Davis, N. (2021, October 18). Valneva Covid vaccine could be as effective as Oxford jab, study suggests. The Guardian. https://www.theguardian.com/society/2021/oct/18/valneva-covid-vaccine-could-be-as-effective-as-oxford-jab-study-suggests

Krause, P. R., Fleming, T. R., Peto, R., Longini, I. M., Figueroa, J. P., Sterne, J. A. C., Cravioto, A., Rees, H., Higgins, J. P. T., Boutron, I., Pan, H., Gruber, M. F., Arora, N., Kazi, F., Gaspar, R., Swaminathan, S., Ryan, M. J., & Henao-Restrepo, A.-M. (2021). Considerations in boosting COVID-19 vaccine immune responses. The Lancet, 4. https://doi.org/10.1016/S0140-6736(21)02046-8

Collier, A. Y., Brown, C. M., Mcmahan, K., Yu, J., Liu, J., Jacob-Dolan, C., Chandrashekar, A., Tierney, D., Ansel, J. L., Rowe, M., Sellers, D., Ahmad, K., Aguayo, R., Anioke, T., Gardner, S., Siamatu, M., Rivera, L. B., Hacker, M. R., Madoff, L. C., & Barouch, D. H. (2021). Immune Responses in Fully Vaccinated Individuals Following Breakthrough Infection with the SARS-CoV-2 Delta Variant in Provincetown, Massachusetts (p. 2021.10.18.21265113). https://doi.org/10.1101/2021.10.18.21265113

John Bye [@_johnbye]. (2021, October 6). The new covid sceptic All Party Parliamentary Group on Pandemic Response and Recovery is backed by Gupta and Heneghan’s Collateral Global to the tune of over £30,000. £5,000 in financial benefits plus £25,501—£27,000 benefits in kind (CG is acting as their secretariat). Https://t.co/qll20Sg9aA [Tweet]. Twitter. https://twitter.com/_johnbye/status/1445867760819396608

Sridhar, D. (2021, September 23). ‘I Know Who Caused Covid-19’ review – the global blame game. The Guardian. https://www.theguardian.com/books/2021/sep/23/i-know-who-caused-covid-19-review-the-global-blame-game

Karan, A. (2022). We cannot afford to repeat these four pandemic mistakes. BMJ, 376, o631. https://doi.org/10.1136/bmj.o631

Prof. Shane Crotty. (2021, October 19). 1/ The regular vaccines give great booster responses against Delta and other variants This was first shown by Moderna, with “original recipe” vaccine booster, compare to Beta booster. Https://nature.com/articles/s41591-021-01527-y https://t.co/g7YDwGIURP [Tweet]. @profshanecrotty. https://twitter.com/profshanecrotty/status/1450489709050744833

Tom Lawton. (2022, January 10). 🔹99.995% of people who drank from that pump didn’t die. 🔹We need to learn to live with the dirty water. 🔹Dirty water is fine to drink if you don’t have underlying health conditions. 🔹It’s not necessary to clean the water unless it’s wholly waterborne. (/S!) [Tweet]. @LawtonTri. https://twitter.com/LawtonTri/status/1480502910459039749

Petersen, M. B., Rasmussen, M. S., Lindholt, M. F., & Jørgensen, F. J. (2021). Pandemic Fatigue and Populism: The Development of Pandemic Fatigue during the COVID-19 Pandemic and How It Fuels Political Discontent across Eight Western Democracies. PsyArXiv. https://doi.org/10.31234/osf.io/y6wm4

What are the atomic building blocks that would allow stimulus-response psychology to show complex behaviors?

How Maker resaponded to the mempool manipulation

Well...OK, this is the gut punch. From my own personal experience on my farm, I feel the same. This morning I saw a a pair of juvenile greater blue herons flying across the creek and then gone. We have at least three nesting pairs of herons on our farm down by the same creek. I feel a wildly inappropriate sense of having helped this brand new mated pair of herons come into being. And then I feel that nothing else in my academic life compares to that. I, too, have wasted my life. It is not a reasonable line of argument. It is a gut feelilng as Wright lays the earlier observational truth upon us. Who can stand in the wake of nature's creative force? Pan always wins.

And the other side of that line is one that says, "It is my life to 'waste'. What you call waste is all of the glorioius connection. A culture that does not value this simple idleness, that condemns it, that is the waste.

This is a salient division to create a series of collective actions: make it visible so that we can respond to the threat in a timely and effective manner.

Mathew, D., Giles, J. R., Baxter, A. E., Oldridge, D. A., Greenplate, A. R., Wu, J. E., Alanio, C., Kuri-Cervantes, L., Pampena, M. B., D’Andrea, K., Manne, S., Chen, Z., Huang, Y. J., Reilly, J. P., Weisman, A. R., Ittner, C. A. G., Kuthuru, O., Dougherty, J., Nzingha, K., … Wherry, E. J. (2020). Deep immune profiling of COVID-19 patients reveals distinct immunotypes with therapeutic implications. Science, 369(6508), eabc8511. https://doi.org/10.1126/science.abc8511

ReconfigBehSci. (2021, November 2). @jeremy_hume personally, I’d consider it a natural, considerate, and moral response to avoid contact with others when carrying an infection that can be dangerous to others. So what seems dystopian to me, is not doing so, and not creating the social/financial conditions that allow it. [Tweet]. @SciBeh. https://twitter.com/SciBeh/status/1455499076850655235

Melton, J., & Sinclair, R. (2021). COVID-19 Infection Rates Are Related to Population Rates of Vaccination: A Response to Subramanian and Kumar.

John Drury [@ProfJohnDrury]. (2022, January 27). What psychology can contribute to pandemic response—Free symposium 15th March https://us06web.zoom.us/webinar/register/WN_24BAGla3R4Kqaqe5YN1qEA New with endorsement by the @BPSOfficial Crisis, disaster and trauma psych section Please share. Https://t.co/FhmBcMBpU2 [Tweet]. Twitter. https://twitter.com/ProfJohnDrury/status/1486719856116256776

Pan-Hammarström lab. (2022, January 7). Our new study: Heterogeneous vaccination strategy with inactivated vaccine + mRNA booster elicits strong B and T cell response against the WT and VOC including Omicron @VirusesImmunity https://t.co/aBiN9anMBL [Tweet]. @panhammarstrom. https://twitter.com/panhammarstrom/status/1479449892410044419

3 big questions about the Biden administration’s COVID response in 2022. (2021, December 30). PBS NewsHour. https://www.pbs.org/newshour/health/3-big-questions-about-the-biden-administrations-covid-response-in-2022

Caulfield, T., Bubela, T., Kimmelman, J., & Ravitsky, V. (2021). Let’s do better: Public representations of COVID-19 science. FACETS, 6, 403–423. https://doi.org/10.1139/facets-2021-0018

Mulot, M., Segalas, C., Leyrat, C., & Besançon, L. (2021). Re: Subramanian and Kumar. Vaccination rates and COVID-19 cases. European Journal of Epidemiology, 36(12), 1243–1244. https://doi.org/10.1007/s10654-021-00817-6

Lee, A., Cheng, K. K., Cruickshank, S., & Greenhalgh, T. (n.d.). “We suppressed our scientific imagination”: Four experts examine the big successes and failures of the COVID response so far. The Conversation. Retrieved March 31, 2022, from http://theconversation.com/we-suppressed-our-scientific-imagination-four-experts-examine-the-big-successes-and-failures-of-the-covid-response-so-far-178705

Trevor Bedford on Twitter. (n.d.). Twitter. Retrieved 29 March 2022, from https://twitter.com/trvrb/status/1466076761427304453

Bago, Bence, David Rand, and Gordon Pennycook. ‘Does Deliberation Decrease Belief in Conspiracies?’ PsyArXiv, 8 March 2022. https://doi.org/10.31234/osf.io/86jhw.

ReconfigBehSci on Twitter: ‘RT @psychmag: This looks very interesting, and several of the speakers have contributed to our Covid coverage https://t.co/pOE34Vy94e and /…’ / Twitter. (n.d.). Retrieved 22 March 2022, from https://twitter.com/SciBeh/status/1486007937818583055

ReconfigBehSci on Twitter: ‘@STWorg @ProfColinDavis @rpancost @chrisdc77 @syrpis this is the most in depth treatment of the impact of equalities law on pandemic policy that I’ve been able to find- it would seem to underscore that there is a legal need for impact assessments that ask (some) of these questions https://t.co/auiApVC0TW’ / Twitter. (n.d.). Retrieved 22 March 2022, from https://twitter.com/SciBeh/status/1485927221449613314

Corbevax: All About India’s First Protein Sub-Unit Covid Vaccine to be Given to 12-14 Age Group From Today. (2022, March 16). News18. https://www.news18.com/news/india/corbevax-all-about-indias-first-protein-sub-unit-covid-vaccine-to-be-given-to-12-14-age-group-from-today-4874345.html